Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Voltage-dependent anion-selective channel protein 3

Gene

Vdac3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi242 – 2443NADBy similarity
Nucleotide bindingi260 – 2645NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

NAD, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent anion-selective channel protein 3
Short name:
VDAC-3
Short name:
rVDAC3
Alternative name(s):
Outer mitochondrial membrane protein porin 3
Gene namesi
Name:Vdac3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621577. Vdac3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 3510Beta strandedBy similarity
Transmembranei39 – 479Beta strandedBy similarity
Transmembranei54 – 6411Beta strandedBy similarityAdd
BLAST
Transmembranei69 – 768Beta strandedBy similarity
Transmembranei80 – 8910Beta strandedBy similarity
Transmembranei95 – 10410Beta strandedBy similarity
Transmembranei111 – 12010Beta strandedBy similarity
Transmembranei123 – 1308Beta strandedBy similarity
Transmembranei137 – 1459Beta strandedBy similarity
Transmembranei150 – 1589Beta strandedBy similarity
Transmembranei163 – 17513Beta strandedBy similarityAdd
BLAST
Transmembranei178 – 1858Beta strandedBy similarity
Transmembranei189 – 19810Beta strandedBy similarity
Transmembranei202 – 21110Beta strandedBy similarity
Transmembranei218 – 22710Beta strandedBy similarity
Transmembranei231 – 2388Beta strandedBy similarity
Transmembranei242 – 25110Beta strandedBy similarity
Transmembranei254 – 26310Beta strandedBy similarity
Transmembranei273 – 28210Beta strandedBy similarity

GO - Cellular componenti

  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
  • pore complex Source: UniProtKB-KW
  • rough endoplasmic reticulum Source: RGD
  • synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 283282Voltage-dependent anion-selective channel protein 3PRO_0000050517Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylcysteineBy similarity
Modified residuei12 – 121N6-acetyllysineBy similarity
Modified residuei15 – 151N6-acetyllysineBy similarity
Modified residuei20 – 201N6-acetyllysine; alternateBy similarity
Modified residuei20 – 201N6-succinyllysine; alternateBy similarity
Modified residuei33 – 331Phosphothreonine1 Publication
Cross-linki53 – 53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki61 – 61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei67 – 671PhosphotyrosineBy similarity
Modified residuei90 – 901N6-acetyllysineBy similarity
Modified residuei109 – 1091N6-acetyllysine; alternateBy similarity
Cross-linki109 – 109Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki110 – 110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki163 – 163Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei241 – 2411Phosphoserine1 Publication
Modified residuei266 – 2661N6-acetyllysine; alternateBy similarity
Cross-linki266 – 266Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki274 – 274Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9R1Z0.
PRIDEiQ9R1Z0.

PTM databases

iPTMnetiQ9R1Z0.
PhosphoSiteiQ9R1Z0.
SwissPalmiQ9R1Z0.

Expressioni

Tissue specificityi

Isoform 1 is widely expressed with strong expression in atrium and ascitic tumor, lower levels in brain and very low levels in liver and kidney. Isoform 2 is also widely expressed with highest levels in brain but no expression in kidney. Also expressed in flagella of epididymal sperm.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi249748. 1 interaction.
STRINGi10116.ENSRNOP00000046203.

Family & Domainsi

Domaini

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ9R1Z0.
KOiK15041.
OrthoDBiEOG7P8P8G.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030271. VDAC3.
[Graphical view]
PANTHERiPTHR11743:SF28. PTHR11743:SF28. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R1Z0-1) [UniParc]FASTAAdd to basket

Also known as: RVDAC3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSTPTYCDL GKAAKDVFNK GYGFGMVKID LKTKSCSGVE FSTSGHAYTD
60 70 80 90 100
TGKASGNLET KYKVCNYGLI FTQKWNTDNT LGTEISWENK LAEGLKLTVD
110 120 130 140 150
TIFVPNTGKK SGKLKASYRR DCFSVGSKVD IDFSGPTIYG WAVLAFEGWL
160 170 180 190 200
AGYQMSFDTA KSKLCQNNFA LGYKAEDFQL HTHVNDGTEF GGSIYQRVNE
210 220 230 240 250
KIETSINLAW TAGSNNTRFG IAAKYRLDCR TSLSAKVNNA SLIGLGYTQS
260 270 280
LRPGVKLTLS ALVDGKNFNA GGHKVGLGFE LEA
Length:283
Mass (Da):30,798
Last modified:August 14, 2001 - v2
Checksum:i38002466B6557864
GO
Isoform 2 (identifier: Q9R1Z0-2) [UniParc]FASTAAdd to basket

Also known as: RVDAC3V

The sequence of this isoform differs from the canonical sequence as follows:
     39-39: V → VM

Show »
Length:284
Mass (Da):30,929
Checksum:i3CB0CD108B7002C6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281K → N in AAF80117 (Ref. 2) Curated
Sequence conflicti128 – 1281K → N in AAH61780 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei39 – 391V → VM in isoform 2. 1 PublicationVSP_005080

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039664 mRNA. Translation: BAB13475.1.
AF268469 mRNA. Translation: AAF80117.1.
BC061780 mRNA. Translation: AAH61780.1.
AF048829 mRNA. Translation: AAD22722.1.
AF048830 mRNA. Translation: AAD22723.1.
RefSeqiNP_112645.1. NM_031355.1. [Q9R1Z0-1]
UniGeneiRn.107287.

Genome annotation databases

GeneIDi83532.
KEGGirno:83532.
UCSCiRGD:621577. rat. [Q9R1Z0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039664 mRNA. Translation: BAB13475.1.
AF268469 mRNA. Translation: AAF80117.1.
BC061780 mRNA. Translation: AAH61780.1.
AF048829 mRNA. Translation: AAD22722.1.
AF048830 mRNA. Translation: AAD22723.1.
RefSeqiNP_112645.1. NM_031355.1. [Q9R1Z0-1]
UniGeneiRn.107287.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249748. 1 interaction.
STRINGi10116.ENSRNOP00000046203.

PTM databases

iPTMnetiQ9R1Z0.
PhosphoSiteiQ9R1Z0.
SwissPalmiQ9R1Z0.

Proteomic databases

PaxDbiQ9R1Z0.
PRIDEiQ9R1Z0.

Protocols and materials databases

DNASUi83532.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83532.
KEGGirno:83532.
UCSCiRGD:621577. rat. [Q9R1Z0-1]

Organism-specific databases

CTDi7419.
RGDi621577. Vdac3.

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
HOGENOMiHOG000188277.
HOVERGENiHBG054036.
InParanoidiQ9R1Z0.
KOiK15041.
OrthoDBiEOG7P8P8G.

Miscellaneous databases

PROiQ9R1Z0.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030271. VDAC3.
[Graphical view]
PANTHERiPTHR11743:SF28. PTHR11743:SF28. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of porin isoforms expressed in tumor cells."
    Shinohara Y., Ishida T., Hino M., Yamazaki N., Baba Y., Terada H.
    Eur. J. Biochem. 267:6067-6073(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Ascitic tumor.
  2. "Ion channels in the lens."
    Rae J.L.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Lens.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Prostate.
  4. "Characterization of rat porin isoforms: cloning of a cardiac type-3 variant encoding an additional methionine at its putative N-terminal region."
    Anflous K., Blondel O., Bernard A., Khrestchatisky M., Ventura-Clapier R.
    Biochim. Biophys. Acta 1399:47-50(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-283 (ISOFORMS 1 AND 2).
    Tissue: Heart.
  5. "Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry."
    Distler A.M., Kerner J., Hoppel C.L.
    Biochim. Biophys. Acta 1774:628-636(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-34 AND 236-245, PHOSPHORYLATION AT THR-33 AND SER-241, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Liver.
  6. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 54-63; 97-109 AND 257-266, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  7. "Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
    Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
    Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiVDAC3_RAT
AccessioniPrimary (citable) accession number: Q9R1Z0
Secondary accession number(s): Q6GSZ1
, Q9ESR2, Q9JI31, Q9WTU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 14, 2001
Last modified: June 8, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.