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Q9R1W5 (CALRL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcitonin gene-related peptide type 1 receptor

Short name=CGRP type 1 receptor
Alternative name(s):
Calcitonin receptor-like receptor
Gene names
Name:Calcrl
Synonyms:Crlr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP2 or RAMP3. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase By similarity.

Subunit structure

Heterodimer of CALCRL and RAMP1, RAMP2 or RAMP3.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed predominantly in the lung, thymus, heart and brain. Ref.1 Ref.3

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 10854696. Source: MGI

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 8582325. Source: MGI

angiogenesis

Inferred from electronic annotation. Source: Ensembl

cAMP biosynthetic process

Inferred from electronic annotation. Source: Ensembl

calcium ion transport

Inferred from electronic annotation. Source: Ensembl

cellular response to sucrose stimulus

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from mutant phenotype PubMed 16537897. Source: MGI

negative regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

positive regulation of cAMP biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 16537897. Source: MGI

positive regulation of smooth muscle cell proliferation

Inferred from mutant phenotype PubMed 16537897. Source: MGI

receptor internalization

Inferred from electronic annotation. Source: Ensembl

regulation of muscle contraction

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentendoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

endosome

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosome

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionadrenomedullin receptor activity

Inferred from electronic annotation. Source: Ensembl

calcitonin gene-related polypeptide receptor activity

Inferred from genetic interaction PubMed 8582325. Source: MGI

calcitonin receptor activity

Inferred from electronic annotation. Source: Ensembl

protein transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 463441Calcitonin gene-related peptide type 1 receptor
PRO_0000012812

Regions

Topological domain23 – 145123Extracellular Potential
Transmembrane146 – 16520Helical; Name=1; Potential
Topological domain166 – 1727Cytoplasmic Potential
Transmembrane173 – 19220Helical; Name=2; Potential
Topological domain193 – 21220Extracellular Potential
Transmembrane213 – 23523Helical; Name=3; Potential
Topological domain236 – 25217Cytoplasmic Potential
Transmembrane253 – 27220Helical; Name=4; Potential
Topological domain273 – 28816Extracellular Potential
Transmembrane289 – 31224Helical; Name=5; Potential
Topological domain313 – 33523Cytoplasmic Potential
Transmembrane336 – 35318Helical; Name=6; Potential
Topological domain354 – 36512Extracellular Potential
Transmembrane366 – 38722Helical; Name=7; Potential
Topological domain388 – 46376Cytoplasmic Potential

Amino acid modifications

Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation651N-linked (GlcNAc...) Potential
Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation1281N-linked (GlcNAc...) Potential
Disulfide bond47 ↔ 73 By similarity
Disulfide bond64 ↔ 104 By similarity
Disulfide bond87 ↔ 126 By similarity

Experimental info

Sequence conflict2351Y → C in BAA76492. Ref.1
Sequence conflict4581M → T in AAD35021. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9R1W5 [UniParc].

Last modified September 26, 2003. Version 2.
Checksum: 630EC8956A4FB47E

FASTA46353,265
        10         20         30         40         50         60 
MDKKHILCFL VLLPLNMALI SAESEEGVNQ TDLGVTRNKI MTAQYECYQK IMQDPIQQAE 

        70         80         90        100        110        120 
GLYCNRTWDG WLCWNDVAAG TESMQYCPDY FQDFDPSEKV TKICDQDGHW FRHPDSNRTW 

       130        140        150        160        170        180 
TNYTLCNNST HEKVKTALNL FYLTIIGHGL SIASLIISLI IFFYFKSLSC QRITLHKNLF 

       190        200        210        220        230        240 
FSFICNSIVT IIHLTAVANN QALVATNPVS CKVSQFIHLY LMGCNYFWML CEGVYLHTLI 

       250        260        270        280        290        300 
VVAVFAEKQH LMWYYFLGWG FPLLPACIHA IARSLYYNDN CWISSDTHLL YIIHGPICAA 

       310        320        330        340        350        360 
LLVNLFFLLN IVRVLITKLK VTHQVESNLY MKAVRATLIL VPLLGIEFVL FPWRPEGKVA 

       370        380        390        400        410        420 
EEVYDYVMHI LMHFQGLLVA TIFCFFNGEV QAILRRNWNQ YKIQFGNGFS HSDALRSASY 

       430        440        450        460 
TVSTISDMQG YSHDCPTEHL NGKSIQDIEN VALKSENMYD LVM 

« Hide

References

[1]"Molecular cloning and characterization of mouse calcitonin gene-related peptide receptor."
Miyauchi K., Tadotsu N., Hayashi T., Ono Y., Tokoyoda K., Tsujikawa K., Yamamoto H.
Neuropeptides 36:22-33(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Spleen.
[2]"Cloning and sequencing of mouse CGRP/adrenomedullin receptor subunits."
Derst C., Preisig-Mueller R., Gerhardus J., Daut J.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Decreased gene expression of adrenomedullin receptor in mouse lungs during sepsis."
Ono Y., Okano I., Kojima M., Okada K., Kangawa K.
Biochem. Biophys. Res. Commun. 271:197-202(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015595 mRNA. Translation: BAA76492.1.
AF146525 mRNA. Translation: AAD35021.1.
AF209905 mRNA. Translation: AAF21037.1.
RefSeqNP_061252.2. NM_018782.2.
XP_006499981.1. XM_006499918.1.
UniGeneMm.75467.

3D structure databases

ProteinModelPortalQ9R1W5.
SMRQ9R1W5. Positions 33-127, 143-387.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2034811.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9R1W5.

Proteomic databases

PaxDbQ9R1W5.
PRIDEQ9R1W5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000074262; ENSMUSP00000073875; ENSMUSG00000059588.
ENSMUST00000099944; ENSMUSP00000097527; ENSMUSG00000059588.
GeneID54598.
KEGGmmu:54598.
UCSCuc008kii.3. mouse.

Organism-specific databases

CTD10203.
MGIMGI:1926944. Calcrl.

Phylogenomic databases

eggNOGNOG255868.
GeneTreeENSGT00750000117654.
HOGENOMHOG000230695.
HOVERGENHBG102129.
InParanoidQ9R1W5.
KOK04577.
OMALCWNDVA.
OrthoDBEOG712TW2.
PhylomeDBQ9R1W5.
TreeFamTF315710.

Gene expression databases

ArrayExpressQ9R1W5.
BgeeQ9R1W5.
CleanExMM_CALCRL.
GenevestigatorQ9R1W5.

Family and domain databases

InterProIPR003287. GCPR_2_calcitonin_rcpt_fam.
IPR017981. GPCR_2-like.
IPR003289. GPCR_2_CGRP1_rcpt.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR01351. CGRPRECEPTOR.
PR01350. CTRFAMILY.
PR00249. GPCRSECRETIN.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311388.
PROQ9R1W5.
SOURCESearch...

Entry information

Entry nameCALRL_MOUSE
AccessionPrimary (citable) accession number: Q9R1W5
Secondary accession number(s): Q9QXH8, Q9WUP2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries