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Protein

Mothers against decapentaplegic homolog 5

Gene

Smad5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651ZincBy similarity
Metal bindingi110 – 1101ZincBy similarity
Metal bindingi122 – 1221ZincBy similarity
Metal bindingi127 – 1271ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-201451. Signaling by BMP.

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 5
Short name:
MAD homolog 5
Short name:
Mothers against DPP homolog 5
Alternative name(s):
SMAD family member 5
Short name:
SMAD 5
Short name:
Smad5
Gene namesi
Name:Smad5
Synonyms:Madh5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi620158. Smad5.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 465464Mothers against decapentaplegic homolog 5PRO_0000090867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei463 – 4631PhosphoserinePROSITE-ProRule annotationBy similarity
Modified residuei465 – 4651PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.By similarity
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9R1V3.

PTM databases

iPTMnetiQ9R1V3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9R1V3. baseline and differential.
GenevisibleiQ9R1V3. RN.

Interactioni

Subunit structurei

May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3. Interacts with WWP1. Interacts with TMEM119.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016704.

Structurei

3D structure databases

ProteinModelPortaliQ9R1V3.
SMRiQ9R1V3. Positions 10-133, 268-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 137125MH1PROSITE-ProRule annotationAdd
BLAST
Domaini271 – 465195MH2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi40 – 467Poly-Lys

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated
Contains 1 MH1 (MAD homology 1) domain.PROSITE-ProRule annotation
Contains 1 MH2 (MAD homology 2) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
GeneTreeiENSGT00760000119091.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiQ9R1V3.
KOiK16790.
OMAiDVQPVEY.
OrthoDBiEOG7W1540.
PhylomeDBiQ9R1V3.
TreeFamiTF314923.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 1 hit.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R1V3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME
60 70 80 90 100
ELEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS
110 120 130 140 150
HHELKPLDIC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHNEFNPQ
160 170 180 190 200
HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP NSTPFPLSPN SPYPPSPASS
210 220 230 240 250
TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMGQDNSQP MDTSNNMIPQ
260 270 280 290 300
IMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT
310 320 330 340 350
DPANNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD
360 370 380 390 400
SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF
410 420 430 440 450
EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV
460
LTQMGSPLNP ISSVS
Length:465
Mass (Da):52,215
Last modified:May 1, 2000 - v1
Checksum:i798F4BC07CC84044
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010955 mRNA. Translation: BAA83093.1.
RefSeqiNP_067724.1. NM_021692.1.
XP_006253648.1. XM_006253586.2.
XP_006253649.1. XM_006253587.2.
XP_006253650.1. XM_006253588.2.
XP_008769668.1. XM_008771446.1.
XP_008769669.1. XM_008771447.1.
XP_008769670.1. XM_008771448.1.
UniGeneiRn.146857.

Genome annotation databases

EnsembliENSRNOT00000016704; ENSRNOP00000016704; ENSRNOG00000022870.
GeneIDi59328.
KEGGirno:59328.
UCSCiRGD:620158. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010955 mRNA. Translation: BAA83093.1.
RefSeqiNP_067724.1. NM_021692.1.
XP_006253648.1. XM_006253586.2.
XP_006253649.1. XM_006253587.2.
XP_006253650.1. XM_006253588.2.
XP_008769668.1. XM_008771446.1.
XP_008769669.1. XM_008771447.1.
XP_008769670.1. XM_008771448.1.
UniGeneiRn.146857.

3D structure databases

ProteinModelPortaliQ9R1V3.
SMRiQ9R1V3. Positions 10-133, 268-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016704.

PTM databases

iPTMnetiQ9R1V3.

Proteomic databases

PaxDbiQ9R1V3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016704; ENSRNOP00000016704; ENSRNOG00000022870.
GeneIDi59328.
KEGGirno:59328.
UCSCiRGD:620158. rat.

Organism-specific databases

CTDi4090.
RGDi620158. Smad5.

Phylogenomic databases

eggNOGiKOG3701. Eukaryota.
ENOG410XQKU. LUCA.
GeneTreeiENSGT00760000119091.
HOGENOMiHOG000286018.
HOVERGENiHBG053353.
InParanoidiQ9R1V3.
KOiK16790.
OMAiDVQPVEY.
OrthoDBiEOG7W1540.
PhylomeDBiQ9R1V3.
TreeFamiTF314923.

Enzyme and pathway databases

ReactomeiR-RNO-201451. Signaling by BMP.

Miscellaneous databases

PROiQ9R1V3.

Gene expression databases

ExpressionAtlasiQ9R1V3. baseline and differential.
GenevisibleiQ9R1V3. RN.

Family and domain databases

Gene3Di2.60.200.10. 1 hit.
3.90.520.10. 1 hit.
InterProiIPR013790. Dwarfin.
IPR003619. MAD_homology1_Dwarfin-type.
IPR013019. MAD_homology_MH1.
IPR017855. SMAD_dom-like.
IPR001132. SMAD_dom_Dwarfin-type.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13703. PTHR13703. 1 hit.
PfamiPF03165. MH1. 1 hit.
PF03166. MH2. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
SM00524. DWB. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF56366. SSF56366. 1 hit.
PROSITEiPS51075. MH1. 1 hit.
PS51076. MH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of rat Smad5 gene."
    Miyakita A., Okuno S., Watanabe T.K., Oga K., Tsuji A., Hishigaki H., Suto T., Nakagawa K., Nakahara Y., Higashi K.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSMAD5_RAT
AccessioniPrimary (citable) accession number: Q9R1V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.