Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Solute carrier family 22 member 8

Gene

Slc22a8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Functions as one of the detoxification systems on the choroid plexus (CP) by removing substrates from the cerebrospinal fluid (CSF). Functions as an organic anion/dicarboxylate exchanger that couples organic anion uptake indirectly to the sodium gradient. Mediates the uptake from brain of organic anions, such as p-aminohippurate (PAH), ochratoxin A, and estrone sulfate (ES) and a cationic compound, cimetidine in a sodium independent manner. Mediates the uptake of benzylpenicillin (PCG), 2,4-dichloro-phenoxyacetate (2,4-D) and PAH in CP. Mediates the efflux of PAH, pravastatin, pitavastatin and PCG from the cerebrum into the blood circulation across the blood-brain barrier (BBB). Involved in regulating the CSF concentration of H(2)-receptor antagonists at the CP. Mediates the renal uptake of indoxyl sulfate (IS), 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) pravastatin, PCG, dehydroepiandrosterone sulfate (DHEAS) and is partly involved in the renal uptake of temocaprilat and ES.10 Publications

GO - Molecular functioni

GO - Biological processi

  • glutathione transport Source: RGD
  • quaternary ammonium group transport Source: RGD
  • response to methotrexate Source: RGD
  • response to toxic substance Source: UniProtKB-KW
  • sodium-independent organic anion transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Detoxification, Ion transport, Transport

Protein family/group databases

TCDBi2.A.1.19.7. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 8
Alternative name(s):
Organic anion transporter 3
Short name:
rOat3
Gene namesi
Name:Slc22a8
Synonyms:Oat3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi632286. Slc22a8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 123ExtracellularSequence analysisAdd BLAST91
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 158CytoplasmicSequence analysisAdd BLAST14
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180ExtracellularSequence analysis1
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 212CytoplasmicSequence analysisAdd BLAST11
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 236ExtracellularSequence analysis3
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 327CytoplasmicSequence analysisAdd BLAST70
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Topological domaini349 – 354ExtracellularSequence analysis6
Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
Topological domaini376 – 383CytoplasmicSequence analysis8
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 411ExtracellularSequence analysis7
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21
Topological domaini433 – 471CytoplasmicSequence analysisAdd BLAST39
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21
Topological domaini493 – 536ExtracellularSequence analysisAdd BLAST44

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2073666.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002734451 – 536Solute carrier family 22 member 8Add BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineCombined sources1
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9R1U7.
PRIDEiQ9R1U7.

PTM databases

iPTMnetiQ9R1U7.
PhosphoSitePlusiQ9R1U7.

Expressioni

Tissue specificityi

Expressed in the liver, brain, kidney, choroid plexus and eye. Moderately expressed in the brain capillary endothelial cells (BCEC).3 Publications

Gene expression databases

BgeeiENSRNOG00000018086.

Interactioni

GO - Molecular functioni

  • protein kinase C binding Source: RGD

Protein-protein interaction databases

BioGridi249719. 1 interactor.
STRINGi10116.ENSRNOP00000024488.

Chemistry databases

BindingDBiQ9R1U7.

Structurei

3D structure databases

ProteinModelPortaliQ9R1U7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ9R1U7.
KOiK08205.
PhylomeDBiQ9R1U7.
TreeFamiTF315847.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1U7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFSEILDRV GSMGPFQYLH VTLLALPVLG IANHNLLQIF TATTPVHHCR
60 70 80 90 100
PPPNASIGPW VLPLDPNGKP EKCLRFVHLP NASLPNDTQR ATEPCLDGWI
110 120 130 140 150
YNSTRDTIVI EWDLVCSSNK LKEMAQSIFM AGILVGGPVI GELSDRFGRK
160 170 180 190 200
PILTWSYLML AASGSGAAFS PSLPVYMIFR FLCGCSISGI SLSTVILNVE
210 220 230 240 250
WVPTSMRAIS STSIGYCYTI GQFILSGLAY AIPQWRWLQL TSSAPFFIFS
260 270 280 290 300
LLSWWVPESI RWLVLSGKYS KALKTLQRVA TFNGKKEEGK KLTIEELKFN
310 320 330 340 350
LQKDITSAKV KYGLSDLFRV SILRRVTFCL SLAWFSTGFA YYSLAMGVEE
360 370 380 390 400
FGVNIYILQI IFGGVDIPAK FITILSLSYL GRRITQSFLL LLAGGAILAL
410 420 430 440 450
IFVPSEMQLL RTALAVFGKG CLSGSFSCLF LYTSELYPTV LRQTGMGISN
460 470 480 490 500
VWARVGSMIA PLVKITGELQ PFIPNVIFGT TALLGGSAAF FLLETLNRPL
510 520 530
PETIEDIQNW HKQVQKTKQE SEAEKASQII PLKTGG
Length:536
Mass (Da):59,186
Last modified:May 1, 2000 - v1
Checksum:iD821E57BFA31F8D9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti515Q → K in AAH81777 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017446 mRNA. Translation: BAA82552.1.
BC081777 mRNA. Translation: AAH81777.1.
RefSeqiNP_112622.1. NM_031332.1.
UniGeneiRn.23348.

Genome annotation databases

GeneIDi83500.
KEGGirno:83500.
UCSCiRGD:632286. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017446 mRNA. Translation: BAA82552.1.
BC081777 mRNA. Translation: AAH81777.1.
RefSeqiNP_112622.1. NM_031332.1.
UniGeneiRn.23348.

3D structure databases

ProteinModelPortaliQ9R1U7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249719. 1 interactor.
STRINGi10116.ENSRNOP00000024488.

Chemistry databases

BindingDBiQ9R1U7.
ChEMBLiCHEMBL2073666.

Protein family/group databases

TCDBi2.A.1.19.7. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ9R1U7.
PhosphoSitePlusiQ9R1U7.

Proteomic databases

PaxDbiQ9R1U7.
PRIDEiQ9R1U7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83500.
KEGGirno:83500.
UCSCiRGD:632286. rat.

Organism-specific databases

CTDi9376.
RGDi632286. Slc22a8.

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ9R1U7.
KOiK08205.
PhylomeDBiQ9R1U7.
TreeFamiTF315847.

Miscellaneous databases

PROiQ9R1U7.

Gene expression databases

BgeeiENSRNOG00000018086.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS22A8_RAT
AccessioniPrimary (citable) accession number: Q9R1U7
Secondary accession number(s): Q66HN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.