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Q9R1T9 (KCNH4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily H member 4
Alternative name(s):
Brain-specific eag-like channel 2
Short name=BEC2
Ether-a-go-go-like potassium channel 1
Short name=ELK channel 1
Short name=rElk1
Voltage-gated potassium channel subunit Kv12.3
Gene names
Name:Kcnh4
Synonyms:Elk1, Elk3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1017 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits an outward current, but shows no inactivation. Channel properties may be modulated by cAMP and subunit assembly.

Subunit structure

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Highly expressed in adult testis, and in adult and embryonic brain. In adult brain found in piriform cortex, olfactory tubercle, cerebral cortex, hippocampus pyramidial cells and dentate gyrus and basal ganglia of caudate/putamen and accumbens nucleus. Detected at intermediate levels in lung, spinal cord, and pituitary. Ref.1 Ref.3

Developmental stage

Expressed at day E18 in embryonic brain.

Domain

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similarities

Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv12.3/KCNH4 sub-subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10171017Potassium voltage-gated channel subfamily H member 4
PRO_0000054009

Regions

Topological domain1 – 232232Cytoplasmic Potential
Transmembrane233 – 25321Helical; Name=Segment S1; Potential
Topological domain254 – 2629Extracellular Potential
Transmembrane263 – 28321Helical; Name=Segment S2; Potential
Topological domain284 – 30522Cytoplasmic Potential
Transmembrane306 – 32621Helical; Name=Segment S3; Potential
Topological domain327 – 3348Extracellular Potential
Transmembrane335 – 35521Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain356 – 3649Cytoplasmic Potential
Transmembrane365 – 38521Helical; Name=Segment S5; Potential
Topological domain386 – 42742Extracellular Potential
Intramembrane428 – 44821Pore-forming; Name=Segment H5; Potential
Topological domain449 – 4546Extracellular Potential
Transmembrane455 – 47521Helical; Name=Segment S6; Potential
Topological domain476 – 1017542Cytoplasmic Potential
Domain14 – 9077PAS
Domain93 – 14553PAC
Nucleotide binding557 – 672116cNMP
Motif440 – 4456Selectivity filter By similarity

Amino acid modifications

Glycosylation4151N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict8251R → W in CAA07587. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9R1T9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: BFDB0F1B35437C9F

FASTA1,017111,404
        10         20         30         40         50         60 
MPVMKGLLAP QNTFLDTIAT RFDGTHSNFL LANAQGPRGF PIVYCSDGFC ELTGYGRTEV 

        70         80         90        100        110        120 
MQKTCSCRFL YGPETSEPAL QRLQKALEGH QEHRAEICFY RKDGSAFWCL LDMMPIKNEL 

       130        140        150        160        170        180 
GEVVLFLFSF KDISQSGGPG LGSPGIHGDN NNHENSLGRR GASSRLRSTR RQNRTVLHRL 

       190        200        210        220        230        240 
TGHFGRRDQG SVKANSNVFE PKPSVPEYKV ASVGGSRCLL LHYSIPKAVW DGLILLATFY 

       250        260        270        280        290        300 
VAVTVPYNVC FAGDDDTPIT SRHTLVSDIA VEMLFILDII LNFRTTYVSQ SGQVVSAPRS 

       310        320        330        340        350        360 
IGLHYLATWF FVDLIAALPF DLLYVFNITV TSLVHLLKTV RLLRLLRLLQ KLERYSQCSA 

       370        380        390        400        410        420 
VVLTLLMSVF ALLAHWMACV WYVIGRREME ANDPLLWDIG WLHELGKRLE EPYVNGSAGG 

       430        440        450        460        470        480 
PSRRSAYIAA LYFTLSSLTS VGFGNVCANT DAEKIFSICT MLIGALMHAV VFGNVTAIIQ 

       490        500        510        520        530        540 
RMYSRRSLYH SRMKDLKDFI RVHRLPRPLK QRMLEYFQTT WAVNSGIDAN ELLRDFPDEL 

       550        560        570        580        590        600 
RADIAMHLNR EILQLPLFGA ASRGCLRALS LHIKTSFCAP GEFLLRRGDA LQAHYYVCSG 

       610        620        630        640        650        660 
SLEVLRDNTV LAILGKGDLI GADIPELGQE PGAGAGCVLK TSADVKALTY CGLQQLSSRG 

       670        680        690        700        710        720 
LAEVLRLYPE YVAAFRAGLP RDLTFNLRQG SENNGLGRFS RSPRLSQARS DTLGSSSDKT 

       730        740        750        760        770        780 
LPSITETEGG MEPGAGSKPR RPLLLPNLSP ARPRGSLVSL LGEELPPFSA LVSSPSLSPT 

       790        800        810        820        830        840 
PSPALAGRGS SPSLHGPPRG SAAWKPPQLL TPPLGTFGPP DLSPRIVDGI EDSSNTAEAP 

       850        860        870        880        890        900 
TFRFSKRPEP TRTRSQAPLS GPRLSRELAT EAAEEVKEKV CRLNQEISRL NQEVSQLSRE 

       910        920        930        940        950        960 
LRQVMGLLQA RLGPPSHPPD STWLPDLPCP HQRPPCISPH MSGPPPGLQN TTLAVVHCPA 

       970        980        990       1000       1010 
SVGTVEIGAT PSELRSSMVP PFPSEPDPLG PSPVPEASPL TPSLLKHSFQ SGSDTFH 

« Hide

References

[1]"Cloning and functional expression of rat ether-a-go-go-like K+ channel genes."
Engeland B., Neu A., Ludwig J., Roeper J., Pongs O.
J. Physiol. (Lond.) 513:647-654(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Brain cortex.
[2]"New ether-a-go-go K+ channel family members localized in human telencephalon."
Miyake A., Mochizuki S., Yokoi H., Kohda M., Furuichi K.
J. Biol. Chem. 274:25018-25025(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain."
Saganich M.J., Machado E., Rudy B.
J. Neurosci. 21:4609-4624(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ007628 mRNA. Translation: CAA07587.1.
AB022699 mRNA. Translation: BAA83593.1.
PIRT31354.
RefSeqNP_446082.1. NM_053630.1.
UniGeneRn.30035.

3D structure databases

ProteinModelPortalQ9R1T9.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9R1T9.
PRIDEQ9R1T9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID114032.
KEGGrno:114032.
UCSCRGD:621415. rat.

Organism-specific databases

CTD23415.
RGD621415. Kcnh4.

Phylogenomic databases

eggNOGCOG2202.
HOGENOMHOG000230794.
HOVERGENHBG052232.
InParanoidQ9R1T9.
KOK04907.
PhylomeDBQ9R1T9.

Gene expression databases

GenevestigatorQ9R1T9.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003950. K_chnl_volt-dep_ELK.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSPR01463. EAGCHANLFMLY.
PR01465. ELKCHANNEL.
SMARTSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio618205.
PROQ9R1T9.

Entry information

Entry nameKCNH4_RAT
AccessionPrimary (citable) accession number: Q9R1T9
Secondary accession number(s): O89048
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families