Q9R1T2 (SAE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SUMO-activating enzyme subunit 1

Alternative name(s):
Ubiquitin-like 1-activating enzyme E1A

Gene names

Name:	Sae1
Synonyms:	Aos1, Sua1, Ubl1a1, Uble1a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 By similarity.
Pathway	Protein modification; protein sumoylation.
Subunit structure	Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Broadly expressed, with highest levels in testis. Ref.4
Sequence similarities	Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein sumoylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	SUMO activating enzyme activity Inferred from electronic annotation. Source: Compara ligase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro protein C-terminus binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9R1T2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9R1T2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     321-350: ALSQRDPPHNNFFFFDGMKGSGIVECLGPQ → VCLGTMCV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	SUMO-activating enzyme subunit 1		PRO_0000194967
Natural variations
	Alternative sequence	321 – 350	30	ALSQR…CLGPQ → VCLGTMCV in isoform 2.		VSP_022004

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 333108F6E98BABAA	FASTA	350	38,620
	10 20 30 40 50 60 MVEKEEAGGG GGGGISEEEA AQYDRQIRLW GLEAQKRLRA SRVLIVGMKG LGAEIAKNLI 70 80 90 100 110 120 LAGVKGLTML DHEQVSPEDP GAQFLIQTGS VGRNRAEASL ERAQNLNPMV DVKVDTEDVE 130 140 150 160 170 180 KKPESFFTKF DAVCLTCCSR DVIIKVDQIC HRNSIKFFTG DVFGYHGYTF ANLGEHEFVE 190 200 210 220 230 240 EKTKVAKVSQ GVEDGPEAKR AKLDSSETTM VKKKVLFCPV KEALEVDWSG EKAKAALKRT 250 260 270 280 290 300 APDYFLLQVL LKFRTDKGRD PTSESYKEDA ELLLQIRNDV FDSLGISPDL LPDDFVRYCF 310 320 330 340 350 SEMAPVCAVV GGILAQEIVK ALSQRDPPHN NFFFFDGMKG SGIVECLGPQ
	Isoform 2 [UniParc]. Checksum: D979A4D5950C2E4F	FASTA	328	36,135
	10 20 30 40 50 60 MVEKEEAGGG GGGGISEEEA AQYDRQIRLW GLEAQKRLRA SRVLIVGMKG LGAEIAKNLI 70 80 90 100 110 120 LAGVKGLTML DHEQVSPEDP GAQFLIQTGS VGRNRAEASL ERAQNLNPMV DVKVDTEDVE 130 140 150 160 170 180 KKPESFFTKF DAVCLTCCSR DVIIKVDQIC HRNSIKFFTG DVFGYHGYTF ANLGEHEFVE 190 200 210 220 230 240 EKTKVAKVSQ GVEDGPEAKR AKLDSSETTM VKKKVLFCPV KEALEVDWSG EKAKAALKRT 250 260 270 280 290 300 APDYFLLQVL LKFRTDKGRD PTSESYKEDA ELLLQIRNDV FDSLGISPDL LPDDFVRYCF 310 320 SEMAPVCAVV GGILAQEIVK VCLGTMCV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mus musculus cDNA similar to human Sua1 complete cds, complete cds." Kaneta Y., Takada S., Itoh M., Takagi N. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: BALB/c, C57BL/6J and NOD. Tissue: Embryo, Embryonic stem cell, Eye and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[4]	"Expression and regulation of the mammalian SUMO-1 E1 enzyme." Azuma Y., Tan S.-H., Cavenagh M.M., Ainsztein A.M., Saitoh H., Dasso M. FASEB J. 15:1825-1827(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB024303 mRNA. Translation: BAA82876.1. AK010313 mRNA. Translation: BAB26845.1. AK011783 mRNA. Translation: BAB27838.1. AK087556 mRNA. Translation: BAC39925.1. AK090012 mRNA. Translation: BAC41044.1. AK154139 mRNA. Translation: BAE32401.1. AK159672 mRNA. Translation: BAE35276.1. AK162789 mRNA. Translation: BAE37060.1. BC068164 mRNA. Translation: AAH68164.1.
IPI	IPI00129105. IPI00816839.
RefSeq	NP_062722.1. NM_019748.2.
UniGene	Mm.258530.
3D structure databases
ProteinModelPortal	Q9R1T2.
SMR	Q9R1T2. Positions 29-349.
ModBase	Search...
PTM databases
PhosphoSite	Q9R1T2.
2D gel databases
REPRODUCTION-2DPAGE	Q9R1T2.
Proteomic databases
PaxDb	Q9R1T2.
PRIDE	Q9R1T2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000094815; ENSMUSP00000092409; ENSMUSG00000052833.
GeneID	56459.
KEGG	mmu:56459.
UCSC	uc009fhp.1. mouse. uc009fhq.1. mouse.
Organism-specific databases
CTD	10055.
MGI	MGI:1929264. Sae1.
Phylogenomic databases
eggNOG	COG0476.
GeneTree	ENSGT00550000075007.
HOGENOM	HOG000172217.
HOVERGEN	HBG080782.
InParanoid	Q9R1T2.
KO	K10684.
OMA	GSGIVEC.
OrthoDB	EOG4FTW0X.
Enzyme and pathway databases
UniPathway	UPA00886.
Gene expression databases
Bgee	Q9R1T2.
CleanEx	MM_SAE1.
Genevestigator	Q9R1T2.
GermOnline	ENSMUSG00000052833. Mus musculus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD-bd. IPR000011. UBQ/SUMO-activ_enz_E1-like. [Graphical view]
Pfam	PF00899. ThiF. 1 hit. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
SUPFAM	SSF69572. MoeB. 1 hit.
ProtoNet	Search...
Other
NextBio	312702.
SOURCE	Search...

Entry information

Entry name

SAE1_MOUSE

Accession

Primary (citable) accession number: Q9R1T2
Secondary accession number(s): Q3TRG9, Q3TWJ0, Q9CSW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program