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Protein

Midkine

Gene

Mdk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • adrenal gland development Source: UniProtKB
  • behavioral fear response Source: Ensembl
  • cell differentiation Source: UniProtKB-KW
  • cell migration Source: RGD
  • cerebellar granular layer development Source: Ensembl
  • cerebral cortex development Source: Ensembl
  • defecation Source: Ensembl
  • dentate gyrus development Source: Ensembl
  • negative regulation of neuron apoptotic process Source: Ensembl
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: Ensembl
  • regulation of behavior Source: Ensembl
  • response to drug Source: RGD
  • response to glucocorticoid Source: RGD
  • response to hormone Source: RGD
  • response to wounding Source: UniProtKB
  • short-term memory Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-RNO-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Midkine
Short name:
MK
Gene namesi
Name:Mdk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi69073. Mdk.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: RGD
  • cytoplasm Source: RGD
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000002466421 – 140MidkineAdd BLAST120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi34 ↔ 58By similarity
Disulfide bondi42 ↔ 67By similarity
Disulfide bondi49 ↔ 71By similarity
Disulfide bondi81 ↔ 113By similarity
Disulfide bondi91 ↔ 123By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9R1S9.
PRIDEiQ9R1S9.

Expressioni

Tissue specificityi

Expressed at a low level in arteries, and at higher levels in newly formed neointima. In brain, expressed in the caudate nucleus and the brain stem.2 Publications

Developmental stagei

In the cortices of the adrenal glands, expressed at 12.5 dpc, decreases considerably at 15.5 dpc and is almost undetectable in the newborn stage. In brain, expressed at low levels in early embryos, expression peaks between E12 and E14, and rapidly falls until birth when expression is barely detectable.2 Publications

Inductioni

In arteries 3 days after injury. Expression continues to increase until day 7 after injury and decreases slightly by day 14.1 Publication

Gene expression databases

BgeeiENSRNOG00000017560.
ExpressionAtlasiQ9R1S9. baseline and differential.
GenevisibleiQ9R1S9. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with ALK.By similarity

Protein-protein interaction databases

BioGridi249514. 3 interactors.
IntActiQ9R1S9. 1 interactor.
STRINGi10116.ENSRNOP00000036207.

Structurei

3D structure databases

ProteinModelPortaliQ9R1S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pleiotrophin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J0E8. Eukaryota.
ENOG4111ZWP. LUCA.
GeneTreeiENSGT00390000007640.
HOVERGENiHBG008317.
InParanoidiQ9R1S9.
KOiK06828.
OMAiYKFESWG.
OrthoDBiEOG091G0VCN.
PhylomeDBiQ9R1S9.

Family and domain databases

Gene3Di2.20.60.10. 1 hit.
2.30.90.10. 1 hit.
InterProiIPR000762. Midkine_heparin-bd_GF.
IPR020090. PTN/MK_C_dom.
IPR020091. PTN/MK_diS.
IPR020089. PTN/MK_N_dom.
IPR020092. PTN_MK_heparin-bd_GF_CS.
[Graphical view]
PANTHERiPTHR13850. PTHR13850. 1 hit.
PfamiPF01091. PTN_MK_C. 1 hit.
PF05196. PTN_MK_N. 1 hit.
[Graphical view]
PRINTSiPR00269. PTNMIDKINE.
ProDomiPD005592. PTN_MK_hepar_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00193. PTN. 1 hit.
[Graphical view]
SUPFAMiSSF57288. SSF57288. 2 hits.
PROSITEiPS00619. PTN_MK_1. 1 hit.
PS00620. PTN_MK_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R1S9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHRSFFLLA LVALLAVTTA VAKKKDKVKK GSECSEWTWG PCTPSSKDCG
60 70 80 90 100
MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR
110 120 130 140
QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD
Length:140
Mass (Da):15,406
Last modified:May 1, 2000 - v1
Checksum:i10E340C43AA619CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025023 mRNA. Translation: BAA83783.1.
AF315950 mRNA. Translation: AAG45151.1.
RefSeqiNP_110486.1. NM_030859.2.
XP_006234654.1. XM_006234592.2.
XP_006234655.1. XM_006234593.3.
UniGeneiRn.17447.

Genome annotation databases

EnsembliENSRNOT00000033978; ENSRNOP00000036207; ENSRNOG00000017560.
GeneIDi81517.
KEGGirno:81517.
UCSCiRGD:69073. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025023 mRNA. Translation: BAA83783.1.
AF315950 mRNA. Translation: AAG45151.1.
RefSeqiNP_110486.1. NM_030859.2.
XP_006234654.1. XM_006234592.2.
XP_006234655.1. XM_006234593.3.
UniGeneiRn.17447.

3D structure databases

ProteinModelPortaliQ9R1S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249514. 3 interactors.
IntActiQ9R1S9. 1 interactor.
STRINGi10116.ENSRNOP00000036207.

Proteomic databases

PaxDbiQ9R1S9.
PRIDEiQ9R1S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033978; ENSRNOP00000036207; ENSRNOG00000017560.
GeneIDi81517.
KEGGirno:81517.
UCSCiRGD:69073. rat.

Organism-specific databases

CTDi4192.
RGDi69073. Mdk.

Phylogenomic databases

eggNOGiENOG410J0E8. Eukaryota.
ENOG4111ZWP. LUCA.
GeneTreeiENSGT00390000007640.
HOVERGENiHBG008317.
InParanoidiQ9R1S9.
KOiK06828.
OMAiYKFESWG.
OrthoDBiEOG091G0VCN.
PhylomeDBiQ9R1S9.

Enzyme and pathway databases

ReactomeiR-RNO-2979096. NOTCH2 Activation and Transmission of Signal to the Nucleus.

Miscellaneous databases

PROiQ9R1S9.

Gene expression databases

BgeeiENSRNOG00000017560.
ExpressionAtlasiQ9R1S9. baseline and differential.
GenevisibleiQ9R1S9. RN.

Family and domain databases

Gene3Di2.20.60.10. 1 hit.
2.30.90.10. 1 hit.
InterProiIPR000762. Midkine_heparin-bd_GF.
IPR020090. PTN/MK_C_dom.
IPR020091. PTN/MK_diS.
IPR020089. PTN/MK_N_dom.
IPR020092. PTN_MK_heparin-bd_GF_CS.
[Graphical view]
PANTHERiPTHR13850. PTHR13850. 1 hit.
PfamiPF01091. PTN_MK_C. 1 hit.
PF05196. PTN_MK_N. 1 hit.
[Graphical view]
PRINTSiPR00269. PTNMIDKINE.
ProDomiPD005592. PTN_MK_hepar_bd. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00193. PTN. 1 hit.
[Graphical view]
SUPFAMiSSF57288. SSF57288. 2 hits.
PROSITEiPS00619. PTN_MK_1. 1 hit.
PS00620. PTN_MK_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMK_RAT
AccessioniPrimary (citable) accession number: Q9R1S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.