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Protein

Tripartite motif-containing protein 3

Gene

Trim3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Positively regulates motility of microtubule-dependent motor protein KIF21B (PubMed:24086586).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 63RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri110 – 151B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • protein ubiquitination Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 3
Alternative name(s):
RING finger protein 22
RING finger protein HAC1
Gene namesi
Name:Trim3
Synonyms:Hac1, Rnf22
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1860040. Trim3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • dendrite Source: UniProtKB-SubCell
  • early endosome Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Endosome, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000561982 – 744Tripartite motif-containing protein 3Add BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei7PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9R1R2.
PaxDbiQ9R1R2.
PRIDEiQ9R1R2.

PTM databases

iPTMnetiQ9R1R2.
PhosphoSitePlusiQ9R1R2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036989.
CleanExiMM_TRIM3.
ExpressionAtlasiQ9R1R2. baseline and differential.
GenevisibleiQ9R1R2. MM.

Interactioni

Subunit structurei

Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4) (By similarity). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3 (By similarity). Interacts with ZFYVE28/LST2 (By similarity). Interacts with KIF21B (PubMed:24086586).By similarity1 Publication

Protein-protein interaction databases

BioGridi207763. 4 interactors.
IntActiQ9R1R2. 5 interactors.
MINTiMINT-4138575.
STRINGi10090.ENSMUSP00000053384.

Structurei

3D structure databases

ProteinModelPortaliQ9R1R2.
SMRiQ9R1R2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati317 – 418FilaminAdd BLAST102
Repeati473 – 516NHL 1Add BLAST44
Repeati520 – 563NHL 2Add BLAST44
Repeati564 – 605NHL 3Add BLAST42
Repeati609 – 652NHL 4Add BLAST44
Repeati656 – 699NHL 5Add BLAST44
Repeati700 – 743NHL 6Add BLAST44

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 290Interaction with KIF21B1 PublicationAdd BLAST289

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili153 – 224Sequence analysisAdd BLAST72

Domaini

The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 filamin repeat.PROSITE-ProRule annotation
Contains 6 NHL repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 63RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri110 – 151B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITF9. Eukaryota.
ENOG410ZAM4. LUCA.
GeneTreeiENSGT00550000074377.
HOGENOMiHOG000220817.
HOVERGENiHBG054843.
InParanoidiQ9R1R2.
KOiK11997.
OMAiCNVLESA.
OrthoDBiEOG091G06IK.
PhylomeDBiQ9R1R2.
TreeFamiTF331018.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR003649. Bbox_C.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 1 hit.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R1R2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKREDSPGP EVQPMDKQFL VCSICLDRYR CPKVLPCLHT FCERCLQNYI
60 70 80 90 100
PPQSLTLSCP VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPEGAHDPE
110 120 130 140 150
DPHPLSAVAG RPLSCPNHEG KTMEFYCEAC ETAMCGECRA GEHREHGTVL
160 170 180 190 200
LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI ALVGGISQQL QERKAEALAQ
210 220 230 240 250
ISAAFEDLEQ ALQQRKQALV SDLESICGAK QKVLQTQLDT LRQGQEHIGS
260 270 280 290 300
SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL
310 320 330 340 350
EVDGLRRSVL NLGALLTTSA TAHETVATGE GLRQALVGQP ASLTVTTKDK
360 370 380 390 400
DGRLVRTGSA ELCAEITGPD GVRLAVPVVD HKNGTYELVY TARTEGDLLL
410 420 430 440 450
SVLLYGQPVR GSPFRVRALR PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV
460 470 480 490 500
RRPSSMYSTG GKRKDNPIED ELVFRVGSRG REKGEFTNLQ GVSAASSGRI
510 520 530 540 550
VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA VDTNGDIIVA
560 570 580 590 600
DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF
610 620 630 640 650
TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS
660 670 680 690 700
ADGEFLFKFG SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG
710 720 730 740
SFLSYINTSA EPLYGPQGLA LTSDGHVVVA DAGNHCFKAY RYLQ
Length:744
Mass (Da):80,774
Last modified:May 1, 2000 - v1
Checksum:iD9AEF4FA264BA168
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030912 mRNA. Translation: BAA83343.1.
AF220019 mRNA. Translation: AAG53473.1.
AK019165 mRNA. Translation: BAB31580.1.
AK144674 mRNA. Translation: BAE26003.1.
BC034263 mRNA. Translation: AAH34263.1.
CCDSiCCDS21655.1.
RefSeqiNP_001272799.1. NM_001285870.1.
NP_001272800.1. NM_001285871.1.
NP_001272802.1. NM_001285873.1.
NP_061368.1. NM_018880.3.
UniGeneiMm.29290.
Mm.326799.

Genome annotation databases

EnsembliENSMUST00000057525; ENSMUSP00000053384; ENSMUSG00000036989.
ENSMUST00000106789; ENSMUSP00000102401; ENSMUSG00000036989.
ENSMUST00000147044; ENSMUSP00000114822; ENSMUSG00000036989.
GeneIDi55992.
KEGGimmu:55992.
UCSCiuc009iym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030912 mRNA. Translation: BAA83343.1.
AF220019 mRNA. Translation: AAG53473.1.
AK019165 mRNA. Translation: BAB31580.1.
AK144674 mRNA. Translation: BAE26003.1.
BC034263 mRNA. Translation: AAH34263.1.
CCDSiCCDS21655.1.
RefSeqiNP_001272799.1. NM_001285870.1.
NP_001272800.1. NM_001285871.1.
NP_001272802.1. NM_001285873.1.
NP_061368.1. NM_018880.3.
UniGeneiMm.29290.
Mm.326799.

3D structure databases

ProteinModelPortaliQ9R1R2.
SMRiQ9R1R2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207763. 4 interactors.
IntActiQ9R1R2. 5 interactors.
MINTiMINT-4138575.
STRINGi10090.ENSMUSP00000053384.

PTM databases

iPTMnetiQ9R1R2.
PhosphoSitePlusiQ9R1R2.

Proteomic databases

MaxQBiQ9R1R2.
PaxDbiQ9R1R2.
PRIDEiQ9R1R2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057525; ENSMUSP00000053384; ENSMUSG00000036989.
ENSMUST00000106789; ENSMUSP00000102401; ENSMUSG00000036989.
ENSMUST00000147044; ENSMUSP00000114822; ENSMUSG00000036989.
GeneIDi55992.
KEGGimmu:55992.
UCSCiuc009iym.2. mouse.

Organism-specific databases

CTDi10612.
MGIiMGI:1860040. Trim3.

Phylogenomic databases

eggNOGiENOG410ITF9. Eukaryota.
ENOG410ZAM4. LUCA.
GeneTreeiENSGT00550000074377.
HOGENOMiHOG000220817.
HOVERGENiHBG054843.
InParanoidiQ9R1R2.
KOiK11997.
OMAiCNVLESA.
OrthoDBiEOG091G06IK.
PhylomeDBiQ9R1R2.
TreeFamiTF331018.

Miscellaneous databases

ChiTaRSiTrim3. mouse.
PROiQ9R1R2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036989.
CleanExiMM_TRIM3.
ExpressionAtlasiQ9R1R2. baseline and differential.
GenevisibleiQ9R1R2. MM.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR003649. Bbox_C.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 1 hit.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRIM3_MOUSE
AccessioniPrimary (citable) accession number: Q9R1R2
Secondary accession number(s): Q3UMU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.