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Protein

V-type proton ATPase subunit S1

Gene

Atp6ap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca2+-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: InterPro
  • cellular iron ion homeostasis Source: UniProtKB
  • cellular response to increased oxygen levels Source: UniProtKB
  • establishment of organelle localization Source: UniProtKB
  • pH reduction Source: UniProtKB
  • positive regulation of bone resorption Source: UniProtKB
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of exocytosis Source: UniProtKB
  • positive regulation of osteoblast differentiation Source: UniProtKB
  • positive regulation of osteoclast development Source: UniProtKB

Keywordsi

Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-77387. Insulin receptor recycling.
R-MMU-917977. Transferrin endocytosis and recycling.
R-MMU-983712. Ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit S1
Short name:
V-ATPase subunit S1
Alternative name(s):
Protein C7-1
V-ATPase Ac45 subunit
V-ATPase S1 accessory protein
Vacuolar proton pump subunit S1
Gene namesi
Name:Atp6ap1
Synonyms:Atp6ip1, Atp6s1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:109629. Atp6ap1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 412VacuolarSequence analysisAdd BLAST380
Transmembranei413 – 433HelicalSequence analysisAdd BLAST21
Topological domaini434 – 463CytoplasmicSequence analysisAdd BLAST30

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000000254433 – 463V-type proton ATPase subunit S1Add BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi164N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi255N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi267N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi344N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi351N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi399N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9R1Q9.
MaxQBiQ9R1Q9.
PaxDbiQ9R1Q9.
PeptideAtlasiQ9R1Q9.
PRIDEiQ9R1Q9.

PTM databases

iPTMnetiQ9R1Q9.
PhosphoSitePlusiQ9R1Q9.

Expressioni

Tissue specificityi

Expressed in brain cortex (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000019087.
ExpressionAtlasiQ9R1Q9. baseline and differential.
GenevisibleiQ9R1Q9. MM.

Interactioni

Subunit structurei

Component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump.By similarity

GO - Molecular functioni

  • Rab GTPase binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000019231.

Structurei

3D structure databases

ProteinModelPortaliQ9R1Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the vacuolar ATPase subunit S1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3868. Eukaryota.
ENOG410XTHY. LUCA.
GeneTreeiENSGT00530000063584.
HOVERGENiHBG001090.
InParanoidiQ9R1Q9.
KOiK03662.
OMAiHITSDMQ.
OrthoDBiEOG091G0772.
TreeFamiTF325819.

Family and domain databases

InterProiView protein in InterPro
IPR008388. ATPase_V1-cplx_s1su.
PANTHERiPTHR12471. PTHR12471. 1 hit.
PfamiView protein in Pfam
PF05827. ATP-synt_S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R1Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAATVVSRI RTGTGRAPVM WLSLSLVAVA AAVATEQQVP LVLWSSDRNL
60 70 80 90 100
WAPVADTHEG HITSDMQLST YLDPALELGP RNVLLFLQDK LSIEDFTAYG
110 120 130 140 150
GVFGNKQDSA FSNLENALDL APSSLVLPAV DWYAISTLTT YLQEKLGASP
160 170 180 190 200
LHVDLATLKE LKLNASLPAL LLIRLPYTAS SGLMAPREVL TGNDEVIGQV
210 220 230 240 250
LSTLKSEDVP YTAALTAVRP SRVARDITMV AGGLGRQLLQ TQVASPAIHP
260 270 280 290 300
PVSYNDTAPR ILFWAQNFSV AYKDEWKDLT SLTFGVENLN LTGSFWNDSF
310 320 330 340 350
AMLSLTYEPL FGATVTFKFI LASRFYPVSA RYWFAMERLE IHSNGSVAHF
360 370 380 390 400
NVSQVTGPSI YSFHCEYVSS VSKKGNLLVT NVPSVWQMTL HNFQIQAFNV
410 420 430 440 450
TGEQFSYASD CAGFFSPGIW MGLLTTLFML FIFTYGLHMI LSLKTMDRFD
460
DHKGPTITLT QIV
Length:463
Mass (Da):51,008
Last modified:May 1, 2000 - v1
Checksum:iAE28D99718BA0AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031290 mRNA. Translation: BAA83498.1.
BC048241 mRNA. Translation: AAH48241.1.
CCDSiCCDS30226.1.
RefSeqiNP_061264.1. NM_018794.4.
UniGeneiMm.21961.

Genome annotation databases

EnsembliENSMUST00000019231; ENSMUSP00000019231; ENSMUSG00000019087.
GeneIDi54411.
KEGGimmu:54411.
UCSCiuc009tol.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiVAS1_MOUSE
AccessioniPrimary (citable) accession number: Q9R1Q9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: September 27, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families