Reviewed, UniProtKB/Swiss-Prot Q9R1P4 (PSA1_MOUSE)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-1
    EC=3.4.25.1
Alternative name(s):
    Proteasome component C2
    Macropain subunit C2
    Multicatalytic endopeptidase complex subunit C2
    Proteasome nu chain

Gene names

Name:

Psma1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm. Nucleus.
Post-translational modification	C-terminal extension is partially cleaved off by limited proteolysis leading to a conversion of the proteasome from its latent into its active form.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NOTCH3	Q9UM47	1	EBI-991653,EBI-1236377	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Proteasome subunit alpha type-1		PRO_0000124062
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	14	1	Phosphoserine By similarity
	Cross-link	115		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	208		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9R1P4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CC791C56AFBA8043	FASTA	263	29,547
	10 20 30 40 50 60 MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK RAQSELAAHQ 70 80 90 100 110 120 KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD RPLPVSRLVS LIGSKTQIPT 130 140 150 160 170 180 QRYGRRPYGV GLLIAGYDDM GPHIFQTCPS ANYFDCRAMS IGARSQSART YLERHMSEFM 190 200 210 220 230 240 ECNLDELVKH GLRALRETLP AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLDGLEERP 250 260 QRKAQPSQAA EEPAEKADEP MEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete primary structure of mouse 20S proteasomes." Elenich L.A., Nandi D., Kent E.A., McCluskey T.S., Cruz M., Iyer M.N., Woodward E.C., Conn C.W., Ochoa A.L., Ginsburg D.B., Monaco J.J. Immunogenetics 49:835-842(1999) [PubMed: 10436176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: B10.A.
[2]	"The mouse Psma1 gene coding for the alpha-type C2 proteasome subunit: structural and functional analysis, mapping, and colocalization with Pde3b on mouse chromosome 7." Hopitzan A., Himmelbauer H., Spevak W., Castanon M.J. Genomics 66:313-323(2000) [PubMed: 10873386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Skeletal muscle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N-3. Tissue: Mammary gland.
[4]	Lubec G., Klug S., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 63-82 AND 97-107, MASS SPECTROMETRY. Tissue: Brain and Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF060088 mRNA. Translation: AAD50533.1. AJ272019 mRNA. Translation: CAB95966.1. AJ272272 Genomic DNA. Translation: CAB95969.1. BC005762 mRNA. Translation: AAH05762.1.
IPI	IPI00283862.
RefSeq	NP_036095.1.
UniGene	Mm.121265
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P40302.
SMR	Q9R1P4. Positions 4-241.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9R1P4. 18 interactions.
STRING	Q9R1P4.
Protein family/group databases
MEROPS	T01.976.
PTM databases
PhosphoSite	Q9R1P4.
2-D gel databases
REPRODUCTION-2DPAGE	Q9R1P4.
Proteomic databases
PRIDE	Q9R1P4.
Genome annotation databases
Ensembl	ENSMUST00000033008; ENSMUSP00000033008; ENSMUSG00000030751; Mus musculus. [Genome view]
GeneID	26440.
KEGG	mmu:26440.
NMPDR	fig|10090.3.peg.17468.
UCSC	uc009jhy.1. mouse.
Organism-specific databases
CTD	26440.
MGI	MGI:1347005. Psma1.
Phylogenomic databases
HOGENOM	Q9R1P4.
HOVERGEN	Q9R1P4.
OMA	YAVEAMK.
Enzyme and pathway databases
BRENDA	3.4.25.1. 244.
Gene expression databases
ArrayExpress	Q9R1P4.
Bgee	Q9R1P4.
Genevestigator	Q9R1P4.
GermOnline	ENSMUSG00000030751. Mus musculus.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	304517.
SOURCE	Search...

Entry information

Entry name

PSA1_MOUSE

Accession

Primary (citable) accession number: Q9R1P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)