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Q9R1M7

- NMD3A_RAT

UniProt

Q9R1M7 - NMD3A_RAT

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Protein

Glutamate receptor ionotropic, NMDA 3A

Gene

Grin3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. May play a role in the development of dendritic spines. May also play a role in PPP2CB-NMDAR mediated signaling mechanism.

GO - Molecular functioni

  1. calcium channel activity Source: Ensembl
  2. extracellular-glutamate-gated ion channel activity Source: RefGenome
  3. glycine binding Source: UniProtKB
  4. ionotropic glutamate receptor activity Source: RGD
  5. neurotransmitter binding Source: RGD
  6. N-methyl-D-aspartate selective glutamate receptor activity Source: RGD
  7. protein phosphatase 2A binding Source: UniProtKB

GO - Biological processi

  1. calcium ion transport Source: UniProtKB
  2. dendrite development Source: Ensembl
  3. ionotropic glutamate receptor signaling pathway Source: RGD
  4. ion transmembrane transport Source: RefGenome
  5. prepulse inhibition Source: Ensembl
  6. response to ethanol Source: Ensembl
  7. rhythmic process Source: RGD
  8. synaptic transmission, glutamatergic Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 3A
Short name:
GluN3A
Alternative name(s):
Glutamate receptor chi-1
N-methyl-D-aspartate receptor
N-methyl-D-aspartate receptor subtype 3A
Short name:
NMDAR3A
Short name:
NR3A
NMDAR-L
NMDAR-L1
Gene namesi
Name:Grin3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621704. Grin3a.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density
Note: Enriched in postsynaptic plasma membrane and postsynaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 674651ExtracellularSequence AnalysisAdd
BLAST
Transmembranei675 – 69521HelicalSequence AnalysisAdd
BLAST
Topological domaini696 – 74853CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei749 – 76921HelicalSequence AnalysisAdd
BLAST
Topological domaini770 – 930161ExtracellularSequence AnalysisAdd
BLAST
Transmembranei931 – 95121HelicalSequence AnalysisAdd
BLAST
Topological domaini952 – 1135184CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. dendrite Source: RefGenome
  3. neuronal cell body Source: Ensembl
  4. N-methyl-D-aspartate selective glutamate receptor complex Source: UniProtKB
  5. postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 11351112Glutamate receptor ionotropic, NMDA 3APRO_0000011569Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi275 – 2751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi549 – 5491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi886 – 8861N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9R1M7.
PRIDEiQ9R1M7.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord.3 Publications

Developmental stagei

Isoform 1 and isoform 2 are expressed in spinal cord, medulla, pons, tegmentum, thalamus and hypothalamus at 15 dpc onwards.3 Publications

Gene expression databases

GenevestigatoriQ9R1M7.

Interactioni

Subunit structurei

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1, GRIN2A or GRIN2B and PPP2CB. Probably interacts with PPP2CB. No complex with PPP2CB is detected when NMDARs are stimulated by NMDA.4 Publications

Protein-protein interaction databases

BioGridi251320. 1 interaction.
IntActiQ9R1M7. 1 interaction.

Structurei

Secondary structure

1
1135
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi513 – 5197Combined sources
Turni523 – 5253Combined sources
Beta strandi526 – 5294Combined sources
Beta strandi540 – 5445Combined sources
Helixi551 – 56212Combined sources
Beta strandi563 – 5653Combined sources
Helixi570 – 5723Combined sources
Beta strandi573 – 5786Combined sources
Helixi579 – 59113Combined sources
Beta strandi593 – 5997Combined sources
Helixi615 – 6217Combined sources
Beta strandi626 – 6283Combined sources
Helixi636 – 6394Combined sources
Beta strandi642 – 6443Combined sources
Beta strandi648 – 65811Combined sources
Helixi783 – 7864Combined sources
Helixi801 – 8099Combined sources
Helixi811 – 8177Combined sources
Helixi818 – 8203Combined sources
Beta strandi822 – 8243Combined sources
Helixi825 – 8339Combined sources
Beta strandi834 – 8363Combined sources
Beta strandi840 – 8456Combined sources
Helixi846 – 8549Combined sources
Beta strandi857 – 8593Combined sources
Beta strandi861 – 8633Combined sources
Beta strandi868 – 8736Combined sources
Beta strandi876 – 8783Combined sources
Helixi883 – 89614Combined sources
Helixi899 – 9079Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RC7X-ray1.58A/B511-660[»]
A/B776-915[»]
2RC8X-ray1.45A/B511-660[»]
A/B776-915[»]
2RC9X-ray1.96A/B511-660[»]
A/B776-915[»]
4KCDX-ray1.68A/B511-660[»]
A/B776-915[»]
ProteinModelPortaliQ9R1M7.
SMRiQ9R1M7. Positions 512-911.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9R1M7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni951 – 98737PPP2CB binding siteAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1080 – 112950Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG320645.
HOGENOMiHOG000231528.
HOVERGENiHBG052634.
InParanoidiQ9R1M7.
KOiK05213.
PhylomeDBiQ9R1M7.
TreeFamiTF314731.

Family and domain databases

InterProiIPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view]
PfamiPF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9R1M7-1) [UniParc]FASTAAdd to Basket

Also known as: NR3-long, NR3-l, NR3A-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRLSLWWLL SRVCLLLPPP CALVLAGVPS SSSHPQPCQI LKRIGHAVRV
60 70 80 90 100
GAVHLQPWTT APRAASRAQE GGRAGAQRDD PESGTWRPPA PSQGARWLGS
110 120 130 140 150
ALHGRGPPGS RKLGEGAGAE TLWPRDALLF AVENLNRVEG LLPYNLSLEV
160 170 180 190 200
VMAIEAGLGD LPLMPFSSPS SPWSSDPFSF LQSVCHTVVV QGVSALLAFP
210 220 230 240 250
QSQGEMMELD LVSSVLHIPV LSIVRHEFPR ESQNPLHLQL SLENSLSSDA
260 270 280 290 300
DVTVSILTMN NWYNFSLLLC QEDWNITDFL LLTENNSKFH LESVINITAN
310 320 330 340 350
LSSTKDLLSF LQVQMDNIRN STPTMVMFGC DMDSIRQIFE MSTQFGLSPP
360 370 380 390 400
ELHWVLGDSQ NVEELRTEGL PLGLIAHGKT TQSVFEYYVQ DAMELVARAV
410 420 430 440 450
ATATMIQPEL ALLPSTMNCM DVKTTNLTSG QYLSRFLANT TFRGLSGSIK
460 470 480 490 500
VKGSTIISSE NNFFIWNLQH DPMGKPMWTR LGSWQGGRIV MDSGIWPEQA
510 520 530 540 550
QRHKTHFQHP NKLHLRVVTL IEHPFVFTRE VDDEGLCPAG QLCLDPMTND
560 570 580 590 600
SSMLDRLFSS LHSSNDTVPI KFKKCCYGYC IDLLEQLAED MNFDFDLYIV
610 620 630 640 650
GDGKYGAWKN GHWTGLVGDL LSGTANMAVT SFSINTARSQ VIDFTSPFFS
660 670 680 690 700
TSLGILVRTR DTAAPIGAFM WPLHWTMWLG IFVALHITAI FLTLYEWKSP
710 720 730 740 750
FGMTPKGRNR NKVFSFSSAL NVCYALLFGR TAAIKPPKCW TGRFLMNLWA
760 770 780 790 800
IFCMFCLSTY TANLAAVMVG EKIYEELSGI HDPKLHHPSQ GFRFGTVRES
810 820 830 840 850
SAEDYVRQSF PEMHEYMRRY NVPATPDGVQ YLKNDPEKLD AFIMDKALLD
860 870 880 890 900
YEVSIDADCK LLTVGKPFAI EGYGIGLPPN SPLTSNISEL ISQYKSHGFM
910 920 930 940 950
DVLHDKWYKV VPCGKRSFAV TETLQMGIKH FSGLFVLLCI GFGLSILTTI
960 970 980 990 1000
GEHIVHRLLL PRIKNKSKLQ YWLHTSQRFH RALNTSFVEE KQPRSKTKRV
1010 1020 1030 1040 1050
EKSRWRRWTC KTEGDSELSL FPRSNLGPQQ LMVWNTSNLS HDNQRKYIFN
1060 1070 1080 1090 1100
DEEGQNQLGT QAHQDIPLPQ RRRELPASLT TNGKADSLNV TRSSVIQELS
1110 1120 1130
ELEKQIQVIR QELQLAVSRK TELEEYQKTN RTCES
Length:1,135
Mass (Da):127,607
Last modified:May 1, 2000 - v1
Checksum:i8EC1FEEAE50AB237
GO
Isoform 2 (identifier: Q9R1M7-2) [UniParc]FASTAAdd to Basket

Also known as: NR3-short, NR3-s

The sequence of this isoform differs from the canonical sequence as follows:
     1003-1022: Missing.

Show »
Length:1,115
Mass (Da):125,171
Checksum:i975DAF3629B60CA1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1003 – 102220Missing in isoform 2. 2 PublicationsVSP_011512Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34938 mRNA. Translation: AAA99501.1.
U29873 mRNA. Translation: AAB58957.1.
AF073379 mRNA. Translation: AAD41650.1.
AF061945 mRNA. Translation: AAD11811.1.
PIRiT31068.
RefSeqiNP_001185512.1. NM_001198583.2. [Q9R1M7-2]
NP_612555.1. NM_138546.2. [Q9R1M7-1]
UniGeneiRn.180226.

Genome annotation databases

GeneIDi191573.
KEGGirno:191573.
UCSCiRGD:621704. rat. [Q9R1M7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34938 mRNA. Translation: AAA99501.1 .
U29873 mRNA. Translation: AAB58957.1 .
AF073379 mRNA. Translation: AAD41650.1 .
AF061945 mRNA. Translation: AAD11811.1 .
PIRi T31068.
RefSeqi NP_001185512.1. NM_001198583.2. [Q9R1M7-2 ]
NP_612555.1. NM_138546.2. [Q9R1M7-1 ]
UniGenei Rn.180226.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2RC7 X-ray 1.58 A/B 511-660 [» ]
A/B 776-915 [» ]
2RC8 X-ray 1.45 A/B 511-660 [» ]
A/B 776-915 [» ]
2RC9 X-ray 1.96 A/B 511-660 [» ]
A/B 776-915 [» ]
4KCD X-ray 1.68 A/B 511-660 [» ]
A/B 776-915 [» ]
ProteinModelPortali Q9R1M7.
SMRi Q9R1M7. Positions 512-911.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 251320. 1 interaction.
IntActi Q9R1M7. 1 interaction.

Chemistry

BindingDBi Q9R1M7.
ChEMBLi CHEMBL1907608.
GuidetoPHARMACOLOGYi 460.

Proteomic databases

PaxDbi Q9R1M7.
PRIDEi Q9R1M7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 191573.
KEGGi rno:191573.
UCSCi RGD:621704. rat. [Q9R1M7-1 ]

Organism-specific databases

CTDi 116443.
RGDi 621704. Grin3a.

Phylogenomic databases

eggNOGi NOG320645.
HOGENOMi HOG000231528.
HOVERGENi HBG052634.
InParanoidi Q9R1M7.
KOi K05213.
PhylomeDBi Q9R1M7.
TreeFami TF314731.

Miscellaneous databases

EvolutionaryTracei Q9R1M7.
NextBioi 622654.
PROi Q9R1M7.

Gene expression databases

Genevestigatori Q9R1M7.

Family and domain databases

InterProi IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
IPR001638. SBP_bac_3.
[Graphical view ]
Pfami PF00060. Lig_chan. 1 hit.
PF00497. SBP_bac_3. 1 hit.
[Graphical view ]
PRINTSi PR00177. NMDARECEPTOR.
SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family."
    Ciabarra A.M., Sullivan J.M., Gahn L.G., Pecht G., Heinemann S., Sevarino K.A.
    J. Neurosci. 15:6498-6508(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
    Tissue: Brain stem and Spinal cord.
  2. "Developmental and regional expression pattern of a novel NMDA receptor-like subunit (NMDAR-L) in the rodent brain."
    Sucher N.J., Akbarian S., Chi C.L., Leclerc C.L., Awobuluyi M., Deitcher D.L., Wu M.K., Yuan J.P., Jones E.G., Lipton S.A.
    J. Neurosci. 15:6509-6520(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Forebrain.
  3. "Altered single channel properties of NMDA receptors containing a novel NMDA receptor subunit splice variant, NR3A-2."
    Perez-Otano I., Contractor A., Schulteis C.T., Gibb A., Heinemann S.F.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  4. "Identification of a long variant of mRNA encoding the NR3 subunit of the NMDA receptor: its regional distribution and developmental expression in the rat brain."
    Sun L., Margolis F.L., Shipley M.T., Lidow M.S.
    FEBS Lett. 441:392-396(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 839-1122 (ISOFORMS 1/2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Brain.
  5. "An NMDA receptor signaling complex with protein phosphatase 2A."
    Chan S.F., Sucher N.J.
    J. Neurosci. 21:7985-7992(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B AND PPP2CB, INTERACTION WITH PPP2CB.
  6. "Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors."
    Perez-Otano I., Schulteis C.T., Contractor A., Lipton S.A., Trimmer J.S., Sucher N.J., Heinemann S.F.
    J. Neurosci. 21:1228-1237(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, GLYCOSYLATION, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B.
  7. "Association of NR3A with the N-methyl-D-aspartate receptor NR1 and NR2 subunits."
    Al-Hallaq R.A., Jarabek B.R., Fu Z., Vicini S., Wolfe B.B., Yasuda R.P.
    Mol. Pharmacol. 62:1119-1127(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B.
  8. "Characterization and comparison of the NR3A subunit of the NMDA receptor in recombinant systems and primary cortical neurons."
    Sasaki Y.F., Rothe T., Premkumar L.S., Das S., Cui J., Talantova M.V., Wong H.-K., Gong X., Chan S.F., Zhang D., Nakanishi N., Sucher N.J., Lipton S.A.
    J. Neurophysiol. 87:2052-2063(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B.

Entry informationi

Entry nameiNMD3A_RAT
AccessioniPrimary (citable) accession number: Q9R1M7
Secondary accession number(s): O09098
, O09155, Q62800, Q63268, Q9Z2H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3