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Q9R1M7

- NMD3A_RAT

UniProt

Q9R1M7 - NMD3A_RAT

Protein

Glutamate receptor ionotropic, NMDA 3A

Gene

Grin3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. May play a role in the development of dendritic spines. May also play a role in PPP2CB-NMDAR mediated signaling mechanism.

    GO - Molecular functioni

    1. calcium channel activity Source: Ensembl
    2. extracellular-glutamate-gated ion channel activity Source: RefGenome
    3. glycine binding Source: UniProtKB
    4. ionotropic glutamate receptor activity Source: RGD
    5. neurotransmitter binding Source: RGD
    6. N-methyl-D-aspartate selective glutamate receptor activity Source: RGD
    7. protein binding Source: UniProtKB
    8. protein phosphatase 2A binding Source: UniProtKB

    GO - Biological processi

    1. calcium ion transport Source: UniProtKB
    2. dendrite development Source: Ensembl
    3. ionotropic glutamate receptor signaling pathway Source: RGD
    4. ion transmembrane transport Source: RefGenome
    5. prepulse inhibition Source: Ensembl
    6. response to ethanol Source: Ensembl
    7. rhythmic process Source: RGD
    8. synaptic transmission, glutamatergic Source: RefGenome

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Calcium, Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate receptor ionotropic, NMDA 3A
    Short name:
    GluN3A
    Alternative name(s):
    Glutamate receptor chi-1
    N-methyl-D-aspartate receptor
    N-methyl-D-aspartate receptor subtype 3A
    Short name:
    NMDAR3A
    Short name:
    NR3A
    NMDAR-L
    NMDAR-L1
    Gene namesi
    Name:Grin3a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621704. Grin3a.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density
    Note: Enriched in postsynaptic plasma membrane and postsynaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membrane.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. dendrite Source: RefGenome
    3. neuronal cell body Source: Ensembl
    4. N-methyl-D-aspartate selective glutamate receptor complex Source: UniProtKB
    5. postsynaptic density Source: UniProtKB-SubCell
    6. postsynaptic membrane Source: RGD

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 11351112Glutamate receptor ionotropic, NMDA 3APRO_0000011569Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi264 – 2641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi275 – 2751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi549 – 5491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi565 – 5651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi886 – 8861N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9R1M7.
    PRIDEiQ9R1M7.

    Expressioni

    Tissue specificityi

    Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord.3 Publications

    Developmental stagei

    Isoform 1 and isoform 2 are expressed in spinal cord, medulla, pons, tegmentum, thalamus and hypothalamus at 15 dpc onwards.3 Publications

    Gene expression databases

    GenevestigatoriQ9R1M7.

    Interactioni

    Subunit structurei

    Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1, GRIN2A or GRIN2B and PPP2CB. Probably interacts with PPP2CB. No complex with PPP2CB is detected when NMDARs are stimulated by NMDA.4 Publications

    Protein-protein interaction databases

    BioGridi251320. 1 interaction.
    IntActiQ9R1M7. 1 interaction.

    Structurei

    Secondary structure

    1
    1135
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi513 – 5197
    Turni523 – 5253
    Beta strandi526 – 5294
    Beta strandi540 – 5445
    Helixi551 – 56212
    Beta strandi563 – 5653
    Helixi570 – 5723
    Beta strandi573 – 5786
    Helixi579 – 59113
    Beta strandi593 – 5997
    Helixi615 – 6217
    Beta strandi626 – 6283
    Helixi636 – 6394
    Beta strandi642 – 6443
    Beta strandi648 – 65811
    Helixi783 – 7864
    Helixi801 – 8099
    Helixi811 – 8177
    Helixi818 – 8203
    Beta strandi822 – 8243
    Helixi825 – 8339
    Beta strandi834 – 8363
    Beta strandi840 – 8456
    Helixi846 – 8549
    Beta strandi857 – 8593
    Beta strandi861 – 8633
    Beta strandi868 – 8736
    Beta strandi876 – 8783
    Helixi883 – 89614
    Helixi899 – 9079

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2RC7X-ray1.58A/B511-660[»]
    A/B776-915[»]
    2RC8X-ray1.45A/B511-660[»]
    A/B776-915[»]
    2RC9X-ray1.96A/B511-660[»]
    A/B776-915[»]
    4KCDX-ray1.68A/B511-660[»]
    A/B776-915[»]
    ProteinModelPortaliQ9R1M7.
    SMRiQ9R1M7. Positions 512-911.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9R1M7.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 674651ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini696 – 74853CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini770 – 930161ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini952 – 1135184CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei675 – 69521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei749 – 76921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei931 – 95121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni951 – 98737PPP2CB binding siteAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1080 – 112950Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG320645.
    HOGENOMiHOG000231528.
    HOVERGENiHBG052634.
    InParanoidiQ9R1M7.
    KOiK05213.
    PhylomeDBiQ9R1M7.
    TreeFamiTF314731.

    Family and domain databases

    InterProiIPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    IPR001638. SBP_bac_3.
    [Graphical view]
    PfamiPF00060. Lig_chan. 1 hit.
    PF00497. SBP_bac_3. 1 hit.
    [Graphical view]
    PRINTSiPR00177. NMDARECEPTOR.
    SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9R1M7-1) [UniParc]FASTAAdd to Basket

    Also known as: NR3-long, NR3-l, NR3A-2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRLSLWWLL SRVCLLLPPP CALVLAGVPS SSSHPQPCQI LKRIGHAVRV     50
    GAVHLQPWTT APRAASRAQE GGRAGAQRDD PESGTWRPPA PSQGARWLGS 100
    ALHGRGPPGS RKLGEGAGAE TLWPRDALLF AVENLNRVEG LLPYNLSLEV 150
    VMAIEAGLGD LPLMPFSSPS SPWSSDPFSF LQSVCHTVVV QGVSALLAFP 200
    QSQGEMMELD LVSSVLHIPV LSIVRHEFPR ESQNPLHLQL SLENSLSSDA 250
    DVTVSILTMN NWYNFSLLLC QEDWNITDFL LLTENNSKFH LESVINITAN 300
    LSSTKDLLSF LQVQMDNIRN STPTMVMFGC DMDSIRQIFE MSTQFGLSPP 350
    ELHWVLGDSQ NVEELRTEGL PLGLIAHGKT TQSVFEYYVQ DAMELVARAV 400
    ATATMIQPEL ALLPSTMNCM DVKTTNLTSG QYLSRFLANT TFRGLSGSIK 450
    VKGSTIISSE NNFFIWNLQH DPMGKPMWTR LGSWQGGRIV MDSGIWPEQA 500
    QRHKTHFQHP NKLHLRVVTL IEHPFVFTRE VDDEGLCPAG QLCLDPMTND 550
    SSMLDRLFSS LHSSNDTVPI KFKKCCYGYC IDLLEQLAED MNFDFDLYIV 600
    GDGKYGAWKN GHWTGLVGDL LSGTANMAVT SFSINTARSQ VIDFTSPFFS 650
    TSLGILVRTR DTAAPIGAFM WPLHWTMWLG IFVALHITAI FLTLYEWKSP 700
    FGMTPKGRNR NKVFSFSSAL NVCYALLFGR TAAIKPPKCW TGRFLMNLWA 750
    IFCMFCLSTY TANLAAVMVG EKIYEELSGI HDPKLHHPSQ GFRFGTVRES 800
    SAEDYVRQSF PEMHEYMRRY NVPATPDGVQ YLKNDPEKLD AFIMDKALLD 850
    YEVSIDADCK LLTVGKPFAI EGYGIGLPPN SPLTSNISEL ISQYKSHGFM 900
    DVLHDKWYKV VPCGKRSFAV TETLQMGIKH FSGLFVLLCI GFGLSILTTI 950
    GEHIVHRLLL PRIKNKSKLQ YWLHTSQRFH RALNTSFVEE KQPRSKTKRV 1000
    EKSRWRRWTC KTEGDSELSL FPRSNLGPQQ LMVWNTSNLS HDNQRKYIFN 1050
    DEEGQNQLGT QAHQDIPLPQ RRRELPASLT TNGKADSLNV TRSSVIQELS 1100
    ELEKQIQVIR QELQLAVSRK TELEEYQKTN RTCES 1135
    Length:1,135
    Mass (Da):127,607
    Last modified:May 1, 2000 - v1
    Checksum:i8EC1FEEAE50AB237
    GO
    Isoform 2 (identifier: Q9R1M7-2) [UniParc]FASTAAdd to Basket

    Also known as: NR3-short, NR3-s

    The sequence of this isoform differs from the canonical sequence as follows:
         1003-1022: Missing.

    Show »
    Length:1,115
    Mass (Da):125,171
    Checksum:i975DAF3629B60CA1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1003 – 102220Missing in isoform 2. 2 PublicationsVSP_011512Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34938 mRNA. Translation: AAA99501.1.
    U29873 mRNA. Translation: AAB58957.1.
    AF073379 mRNA. Translation: AAD41650.1.
    AF061945 mRNA. Translation: AAD11811.1.
    PIRiT31068.
    RefSeqiNP_001185512.1. NM_001198583.1. [Q9R1M7-2]
    NP_612555.1. NM_138546.1. [Q9R1M7-1]
    UniGeneiRn.180226.

    Genome annotation databases

    GeneIDi191573.
    KEGGirno:191573.
    UCSCiRGD:621704. rat. [Q9R1M7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34938 mRNA. Translation: AAA99501.1 .
    U29873 mRNA. Translation: AAB58957.1 .
    AF073379 mRNA. Translation: AAD41650.1 .
    AF061945 mRNA. Translation: AAD11811.1 .
    PIRi T31068.
    RefSeqi NP_001185512.1. NM_001198583.1. [Q9R1M7-2 ]
    NP_612555.1. NM_138546.1. [Q9R1M7-1 ]
    UniGenei Rn.180226.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2RC7 X-ray 1.58 A/B 511-660 [» ]
    A/B 776-915 [» ]
    2RC8 X-ray 1.45 A/B 511-660 [» ]
    A/B 776-915 [» ]
    2RC9 X-ray 1.96 A/B 511-660 [» ]
    A/B 776-915 [» ]
    4KCD X-ray 1.68 A/B 511-660 [» ]
    A/B 776-915 [» ]
    ProteinModelPortali Q9R1M7.
    SMRi Q9R1M7. Positions 512-911.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 251320. 1 interaction.
    IntActi Q9R1M7. 1 interaction.

    Chemistry

    BindingDBi Q9R1M7.
    ChEMBLi CHEMBL1907608.
    GuidetoPHARMACOLOGYi 460.

    Proteomic databases

    PaxDbi Q9R1M7.
    PRIDEi Q9R1M7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 191573.
    KEGGi rno:191573.
    UCSCi RGD:621704. rat. [Q9R1M7-1 ]

    Organism-specific databases

    CTDi 116443.
    RGDi 621704. Grin3a.

    Phylogenomic databases

    eggNOGi NOG320645.
    HOGENOMi HOG000231528.
    HOVERGENi HBG052634.
    InParanoidi Q9R1M7.
    KOi K05213.
    PhylomeDBi Q9R1M7.
    TreeFami TF314731.

    Miscellaneous databases

    EvolutionaryTracei Q9R1M7.
    NextBioi 622654.
    PROi Q9R1M7.

    Gene expression databases

    Genevestigatori Q9R1M7.

    Family and domain databases

    InterProi IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    IPR001638. SBP_bac_3.
    [Graphical view ]
    Pfami PF00060. Lig_chan. 1 hit.
    PF00497. SBP_bac_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00177. NMDARECEPTOR.
    SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family."
      Ciabarra A.M., Sullivan J.M., Gahn L.G., Pecht G., Heinemann S., Sevarino K.A.
      J. Neurosci. 15:6498-6508(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Sprague-Dawley.
      Tissue: Brain stem and Spinal cord.
    2. "Developmental and regional expression pattern of a novel NMDA receptor-like subunit (NMDAR-L) in the rodent brain."
      Sucher N.J., Akbarian S., Chi C.L., Leclerc C.L., Awobuluyi M., Deitcher D.L., Wu M.K., Yuan J.P., Jones E.G., Lipton S.A.
      J. Neurosci. 15:6509-6520(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Forebrain.
    3. "Altered single channel properties of NMDA receptors containing a novel NMDA receptor subunit splice variant, NR3A-2."
      Perez-Otano I., Contractor A., Schulteis C.T., Gibb A., Heinemann S.F.
      Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    4. "Identification of a long variant of mRNA encoding the NR3 subunit of the NMDA receptor: its regional distribution and developmental expression in the rat brain."
      Sun L., Margolis F.L., Shipley M.T., Lidow M.S.
      FEBS Lett. 441:392-396(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 839-1122 (ISOFORMS 1/2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Brain.
    5. "An NMDA receptor signaling complex with protein phosphatase 2A."
      Chan S.F., Sucher N.J.
      J. Neurosci. 21:7985-7992(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B AND PPP2CB, INTERACTION WITH PPP2CB.
    6. "Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors."
      Perez-Otano I., Schulteis C.T., Contractor A., Lipton S.A., Trimmer J.S., Sucher N.J., Heinemann S.F.
      J. Neurosci. 21:1228-1237(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, GLYCOSYLATION, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B.
    7. "Association of NR3A with the N-methyl-D-aspartate receptor NR1 and NR2 subunits."
      Al-Hallaq R.A., Jarabek B.R., Fu Z., Vicini S., Wolfe B.B., Yasuda R.P.
      Mol. Pharmacol. 62:1119-1127(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B.
    8. "Characterization and comparison of the NR3A subunit of the NMDA receptor in recombinant systems and primary cortical neurons."
      Sasaki Y.F., Rothe T., Premkumar L.S., Das S., Cui J., Talantova M.V., Wong H.-K., Gong X., Chan S.F., Zhang D., Nakanishi N., Sucher N.J., Lipton S.A.
      J. Neurophysiol. 87:2052-2063(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), IDENTIFICATION IN A COMPLEX WITH GRIN1; GRIN2A OR GRIN2B.

    Entry informationi

    Entry nameiNMD3A_RAT
    AccessioniPrimary (citable) accession number: Q9R1M7
    Secondary accession number(s): O09098
    , O09155, Q62800, Q63268, Q9Z2H0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3