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Protein

Glutamate receptor ionotropic, NMDA 3A

Gene

Grin3a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. May play a role in the development of dendritic spines. May also play a role in PPP2CB-NMDAR mediated signaling mechanism.

GO - Molecular functioni

  • extracellular-glutamate-gated ion channel activity Source: InterPro
  • glycine binding Source: UniProtKB
  • ionotropic glutamate receptor activity Source: RGD
  • neurotransmitter binding Source: RGD
  • NMDA glutamate receptor activity Source: RGD
  • protein phosphatase 2A binding Source: UniProtKB

GO - Biological processi

  • calcium ion transport Source: UniProtKB
  • ionotropic glutamate receptor signaling pathway Source: RGD
  • rhythmic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 3A
Short name:
GluN3A
Alternative name(s):
Glutamate receptor chi-1
N-methyl-D-aspartate receptor
N-methyl-D-aspartate receptor subtype 3A
Short name:
NMDAR3A
Short name:
NR3A
NMDAR-L
NMDAR-L1
Gene namesi
Name:Grin3a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621704. Grin3a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 674ExtracellularSequence analysisAdd BLAST651
Transmembranei675 – 695HelicalSequence analysisAdd BLAST21
Topological domaini696 – 748CytoplasmicSequence analysisAdd BLAST53
Transmembranei749 – 769HelicalSequence analysisAdd BLAST21
Topological domaini770 – 930ExtracellularSequence analysisAdd BLAST161
Transmembranei931 – 951HelicalSequence analysisAdd BLAST21
Topological domaini952 – 1135CytoplasmicSequence analysisAdd BLAST184

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • NMDA selective glutamate receptor complex Source: UniProtKB
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL342.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001156924 – 1135Glutamate receptor ionotropic, NMDA 3AAdd BLAST1112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi145N-linked (GlcNAc...)Sequence analysis1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1
Glycosylationi275N-linked (GlcNAc...)Sequence analysis1
Glycosylationi285N-linked (GlcNAc...)Sequence analysis1
Glycosylationi296N-linked (GlcNAc...)Sequence analysis1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Glycosylationi426N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Glycosylationi549N-linked (GlcNAc...)Sequence analysis1
Glycosylationi565N-linked (GlcNAc...)Sequence analysis1
Glycosylationi886N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9R1M7.
PRIDEiQ9R1M7.

PTM databases

iPTMnetiQ9R1M7.
PhosphoSitePlusiQ9R1M7.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in olfactory bulb, frontal occipital, entorhinal and pyriform cortices, hippocampus, striatum, thalamus, cerebellum and spinal cord.3 Publications

Developmental stagei

Isoform 1 and isoform 2 are expressed in spinal cord, medulla, pons, tegmentum, thalamus and hypothalamus at 15 dpc onwards.3 Publications

Gene expression databases

BgeeiENSRNOG00000005723.

Interactioni

Subunit structurei

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1, GRIN2A or GRIN2B and PPP2CB. Probably interacts with PPP2CB. No complex with PPP2CB is detected when NMDARs are stimulated by NMDA.4 Publications

GO - Molecular functioni

  • protein phosphatase 2A binding Source: UniProtKB

Protein-protein interaction databases

BioGridi251320. 1 interactor.
IntActiQ9R1M7. 1 interactor.
STRINGi10116.ENSRNOP00000007957.

Chemistry databases

BindingDBiQ9R1M7.

Structurei

Secondary structure

11135
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi513 – 519Combined sources7
Turni523 – 525Combined sources3
Beta strandi526 – 529Combined sources4
Beta strandi540 – 544Combined sources5
Helixi551 – 562Combined sources12
Beta strandi563 – 565Combined sources3
Helixi570 – 572Combined sources3
Beta strandi573 – 578Combined sources6
Helixi579 – 591Combined sources13
Beta strandi593 – 599Combined sources7
Helixi615 – 621Combined sources7
Beta strandi626 – 628Combined sources3
Helixi636 – 639Combined sources4
Beta strandi642 – 644Combined sources3
Beta strandi648 – 658Combined sources11
Helixi783 – 786Combined sources4
Helixi801 – 809Combined sources9
Helixi811 – 817Combined sources7
Helixi818 – 820Combined sources3
Beta strandi822 – 824Combined sources3
Helixi825 – 833Combined sources9
Beta strandi834 – 836Combined sources3
Beta strandi840 – 845Combined sources6
Helixi846 – 854Combined sources9
Beta strandi857 – 859Combined sources3
Beta strandi861 – 863Combined sources3
Beta strandi868 – 873Combined sources6
Beta strandi876 – 878Combined sources3
Helixi883 – 896Combined sources14
Helixi899 – 907Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RC7X-ray1.58A/B511-660[»]
A/B776-915[»]
2RC8X-ray1.45A/B511-660[»]
A/B776-915[»]
2RC9X-ray1.96A/B511-660[»]
A/B776-915[»]
4KCDX-ray1.68A/B511-660[»]
A/B776-915[»]
ProteinModelPortaliQ9R1M7.
SMRiQ9R1M7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9R1M7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni951 – 987PPP2CB binding siteAdd BLAST37

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1080 – 1129Sequence analysisAdd BLAST50

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1053. Eukaryota.
ENOG410XNUR. LUCA.
HOGENOMiHOG000231528.
HOVERGENiHBG052634.
InParanoidiQ9R1M7.
KOiK05213.
PhylomeDBiQ9R1M7.
TreeFamiTF314731.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R1M7-1) [UniParc]FASTAAdd to basket
Also known as: NR3-long, NR3-l, NR3A-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRLSLWWLL SRVCLLLPPP CALVLAGVPS SSSHPQPCQI LKRIGHAVRV
60 70 80 90 100
GAVHLQPWTT APRAASRAQE GGRAGAQRDD PESGTWRPPA PSQGARWLGS
110 120 130 140 150
ALHGRGPPGS RKLGEGAGAE TLWPRDALLF AVENLNRVEG LLPYNLSLEV
160 170 180 190 200
VMAIEAGLGD LPLMPFSSPS SPWSSDPFSF LQSVCHTVVV QGVSALLAFP
210 220 230 240 250
QSQGEMMELD LVSSVLHIPV LSIVRHEFPR ESQNPLHLQL SLENSLSSDA
260 270 280 290 300
DVTVSILTMN NWYNFSLLLC QEDWNITDFL LLTENNSKFH LESVINITAN
310 320 330 340 350
LSSTKDLLSF LQVQMDNIRN STPTMVMFGC DMDSIRQIFE MSTQFGLSPP
360 370 380 390 400
ELHWVLGDSQ NVEELRTEGL PLGLIAHGKT TQSVFEYYVQ DAMELVARAV
410 420 430 440 450
ATATMIQPEL ALLPSTMNCM DVKTTNLTSG QYLSRFLANT TFRGLSGSIK
460 470 480 490 500
VKGSTIISSE NNFFIWNLQH DPMGKPMWTR LGSWQGGRIV MDSGIWPEQA
510 520 530 540 550
QRHKTHFQHP NKLHLRVVTL IEHPFVFTRE VDDEGLCPAG QLCLDPMTND
560 570 580 590 600
SSMLDRLFSS LHSSNDTVPI KFKKCCYGYC IDLLEQLAED MNFDFDLYIV
610 620 630 640 650
GDGKYGAWKN GHWTGLVGDL LSGTANMAVT SFSINTARSQ VIDFTSPFFS
660 670 680 690 700
TSLGILVRTR DTAAPIGAFM WPLHWTMWLG IFVALHITAI FLTLYEWKSP
710 720 730 740 750
FGMTPKGRNR NKVFSFSSAL NVCYALLFGR TAAIKPPKCW TGRFLMNLWA
760 770 780 790 800
IFCMFCLSTY TANLAAVMVG EKIYEELSGI HDPKLHHPSQ GFRFGTVRES
810 820 830 840 850
SAEDYVRQSF PEMHEYMRRY NVPATPDGVQ YLKNDPEKLD AFIMDKALLD
860 870 880 890 900
YEVSIDADCK LLTVGKPFAI EGYGIGLPPN SPLTSNISEL ISQYKSHGFM
910 920 930 940 950
DVLHDKWYKV VPCGKRSFAV TETLQMGIKH FSGLFVLLCI GFGLSILTTI
960 970 980 990 1000
GEHIVHRLLL PRIKNKSKLQ YWLHTSQRFH RALNTSFVEE KQPRSKTKRV
1010 1020 1030 1040 1050
EKSRWRRWTC KTEGDSELSL FPRSNLGPQQ LMVWNTSNLS HDNQRKYIFN
1060 1070 1080 1090 1100
DEEGQNQLGT QAHQDIPLPQ RRRELPASLT TNGKADSLNV TRSSVIQELS
1110 1120 1130
ELEKQIQVIR QELQLAVSRK TELEEYQKTN RTCES
Length:1,135
Mass (Da):127,607
Last modified:May 1, 2000 - v1
Checksum:i8EC1FEEAE50AB237
GO
Isoform 2 (identifier: Q9R1M7-2) [UniParc]FASTAAdd to basket
Also known as: NR3-short, NR3-s

The sequence of this isoform differs from the canonical sequence as follows:
     1003-1022: Missing.

Show »
Length:1,115
Mass (Da):125,171
Checksum:i975DAF3629B60CA1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0115121003 – 1022Missing in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34938 mRNA. Translation: AAA99501.1.
U29873 mRNA. Translation: AAB58957.1.
AF073379 mRNA. Translation: AAD41650.1.
AF061945 mRNA. Translation: AAD11811.1.
PIRiT31068.
RefSeqiNP_001185512.1. NM_001198583.2. [Q9R1M7-2]
NP_612555.1. NM_138546.2. [Q9R1M7-1]
UniGeneiRn.180226.

Genome annotation databases

GeneIDi191573.
KEGGirno:191573.
UCSCiRGD:621704. rat. [Q9R1M7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34938 mRNA. Translation: AAA99501.1.
U29873 mRNA. Translation: AAB58957.1.
AF073379 mRNA. Translation: AAD41650.1.
AF061945 mRNA. Translation: AAD11811.1.
PIRiT31068.
RefSeqiNP_001185512.1. NM_001198583.2. [Q9R1M7-2]
NP_612555.1. NM_138546.2. [Q9R1M7-1]
UniGeneiRn.180226.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RC7X-ray1.58A/B511-660[»]
A/B776-915[»]
2RC8X-ray1.45A/B511-660[»]
A/B776-915[»]
2RC9X-ray1.96A/B511-660[»]
A/B776-915[»]
4KCDX-ray1.68A/B511-660[»]
A/B776-915[»]
ProteinModelPortaliQ9R1M7.
SMRiQ9R1M7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251320. 1 interactor.
IntActiQ9R1M7. 1 interactor.
STRINGi10116.ENSRNOP00000007957.

Chemistry databases

BindingDBiQ9R1M7.
ChEMBLiCHEMBL342.

PTM databases

iPTMnetiQ9R1M7.
PhosphoSitePlusiQ9R1M7.

Proteomic databases

PaxDbiQ9R1M7.
PRIDEiQ9R1M7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi191573.
KEGGirno:191573.
UCSCiRGD:621704. rat. [Q9R1M7-1]

Organism-specific databases

CTDi116443.
RGDi621704. Grin3a.

Phylogenomic databases

eggNOGiKOG1053. Eukaryota.
ENOG410XNUR. LUCA.
HOGENOMiHOG000231528.
HOVERGENiHBG052634.
InParanoidiQ9R1M7.
KOiK05213.
PhylomeDBiQ9R1M7.
TreeFamiTF314731.

Miscellaneous databases

EvolutionaryTraceiQ9R1M7.
PROiQ9R1M7.

Gene expression databases

BgeeiENSRNOG00000005723.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNMD3A_RAT
AccessioniPrimary (citable) accession number: Q9R1M7
Secondary accession number(s): O09098
, O09155, Q62800, Q63268, Q9Z2H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.