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Protein

NACHT, LRR and PYD domains-containing protein 5

Gene

Nlrp5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi249 – 256ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • cellular protein complex assembly Source: MGI
  • cellular protein localization Source: MGI
  • embryo implantation Source: MGI
  • fertilization Source: MGI
  • in utero embryonic development Source: MGI
  • neuron death Source: MGI
  • regulation of protein stability Source: MGI
  • regulation of RNA stability Source: MGI
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NACHT, LRR and PYD domains-containing protein 5
Alternative name(s):
Maternal antigen that embryos require
Short name:
Mater protein
Ooplasm-specific protein 1
Short name:
OP1
Gene namesi
Name:Nlrp5
Synonyms:Mater, Nalp5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1345193. Nlrp5.

Subcellular locationi

GO - Cellular componenti

  • apical cortex Source: MGI
  • cell cortex Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • mitochondrion Source: UniProtKB
  • nucleolus Source: UniProtKB
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice appear phenotypically normal with no apparent defects in ovarian development, oocyte maturation or ovulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000808911 – 1163NACHT, LRR and PYD domains-containing protein 5Add BLAST1163

Proteomic databases

PaxDbiQ9R1M5.
PRIDEiQ9R1M5.

PTM databases

iPTMnetiQ9R1M5.
PhosphoSitePlusiQ9R1M5.

Expressioni

Tissue specificityi

Oocyte-specific.1 Publication

Developmental stagei

Expression is first detected in oocytes of type 3A primary follicles. Transcripts accumulate during oogenesis. During meiotic maturation, the vast majority of the transcripts are degraded and virtually none is detected by 2-cell stage embryogenesis. The protein however persists during preimplantation up to the blastocyst stage. At 2-cell stage, excluded from cell-cell contact regions. Continuous exclusion from these regions during preimplantation development leads to the absence of the protein from the inner cells of the morula and the inner cell mass of the blastocyst.2 Publications

Gene expression databases

BgeeiENSMUSG00000015721.
CleanExiMM_NLRP5.
GenevisibleiQ9R1M5. MM.

Interactioni

Subunit structurei

Component of the SCMC which also includes KHDC3, OOEP and TLE6. Within the complex, interacts with KHDC3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Khdc3Q9CWU53EBI-2905719,EBI-2905804

Protein-protein interaction databases

BioGridi204829. 1 interactor.
IntActiQ9R1M5. 3 interactors.
STRINGi10090.ENSMUSP00000015866.

Structurei

3D structure databases

ProteinModelPortaliQ9R1M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini243 – 565NACHTPROSITE-ProRule annotationAdd BLAST323
Repeati801 – 822LRR 1Add BLAST22
Repeati830 – 851LRR 2Add BLAST22
Repeati858 – 878LRR 3Add BLAST21
Repeati887 – 906LRR 4Add BLAST20
Repeati915 – 935LRR 5Add BLAST21
Repeati944 – 964LRR 6Add BLAST21
Repeati972 – 993LRR 7Add BLAST22
Repeati1001 – 1022LRR 8Add BLAST22
Repeati1029 – 1050LRR 9Add BLAST22
Repeati1058 – 1079LRR 10Add BLAST22
Repeati1086 – 1107LRR 11Add BLAST22

Sequence similaritiesi

Contains 11 LRR (leucine-rich) repeats.Curated
Contains 1 NACHT domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IIY8. Eukaryota.
ENOG4111BJJ. LUCA.
GeneTreeiENSGT00860000133669.
HOVERGENiHBG063656.
InParanoidiQ9R1M5.
OMAiMTSPEND.
OrthoDBiEOG091G01CG.
PhylomeDBiQ9R1M5.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 4 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]

Sequences (8)i

Sequence statusi: Complete.

This entry describes 8 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R1M5-1) [UniParc]FASTAAdd to basket
Also known as: D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPPEKESKA ILKARGLEEE QKSERKMTSP ENDSKSIQKD QGPEQEQTSE
60 70 80 90 100
STMGPPEKES KAILKARGLE EEQKSERKMT SPENDSKSIQ KDQGPEQEQT
110 120 130 140 150
SESTMGPPEK DSKAILKARG LEEEQKSEST MSPSENVSRA ILKDSGSEEV
160 170 180 190 200
EQASERKMTS PENDSKSIQK DQGPEQEQTS ETLQSKEEDE VTEADKDNGG
210 220 230 240 250
DLQDYKAHVI AKFDTSVDLH YDSPEMKLLS DAFKPYQKTF QPHTIILHGR
260 270 280 290 300
PGVGKSALAR SIVLGWAQGK LFQKMSFVIF FSVREIKWTE KSSLAQLIAK
310 320 330 340 350
ECPDSWDLVT KIMSQPERLL FVIDGLDDMD SVLQHDDMTL SRDWKDEQPI
360 370 380 390 400
YILMYSLLRK ALLPQSFLII TTRNTGLEKL KSMVVSPLYI LVEGLSASRR
410 420 430 440 450
SQLVLENISN ESDRIQVFHS LIENHQLFDQ CQAPSVCSLV CEALQLQKKL
460 470 480 490 500
GKRCTLPCQT LTGLYATLVF HQLTLKRPSQ SALSQEEQIT LVGLCMMAAE
510 520 530 540 550
GVWTMRSVFY DDDLKNYSLK ESEILALFHM NILLQVGHNS EQCYVFSHLS
560 570 580 590 600
LQDFFAALYY VLEGLEEWNQ HFCFIENQRS IMEVKRTDDT RLLGMKRFLF
610 620 630 640 650
GLMNKDILKT LEVLFEYPVI PTVEQKLQHW VSLIAQQVNG TSPMDTLDAF
660 670 680 690 700
YCLFESQDEE FVGGALKRFQ EVWLLINQKM DLKVSSYCLK HCQNLKAIRV
710 720 730 740 750
DIRDLLSVDN TLELCPVVTV QETQCKPLLM EWWGNFCSVL GSLRNLKELD
760 770 780 790 800
LGDSILSQRA MKILCLELRN QSCRIQKLTF KSAEVVSGLK HLWKLLFSNQ
810 820 830 840 850
NLKYLNLGNT PMKDDDMKLA CEALKHPKCS VETLRLDSCE LTIIGYEMIS
860 870 880 890 900
TLLISTTRLK CLSLAKNRVG VKSMISLGNA LSSSMCLLQK LILDNCGLTP
910 920 930 940 950
ASCHLLVSAL FSNQNLTHLC LSNNSLGTEG VQQLCQFLRN PECALQRLIL
960 970 980 990 1000
NHCNIVDDAY GFLAMRLANN TKLTHLSLTM NPVGDGAMKL LCEALKEPTC
1010 1020 1030 1040 1050
YLQELELVDC QLTQNCCEDL ACMITTTKHL KSLDLGNNAL GDKGVITLCE
1060 1070 1080 1090 1100
GLKQSSSSLR RLGLGACKLT SNCCEALSLA ISCNPHLNSL NLVKNDFSTS
1110 1120 1130 1140 1150
GMLKLCSAFQ CPVSNLGIIG LWKQEYYARV RRQLEEVEFV KPHVVIDGDW
1160
YASDEDDRNW WKN
Length:1,163
Mass (Da):131,318
Last modified:May 1, 2007 - v2
Checksum:iEF14379B454E9376
GO
Isoform 2 (identifier: Q9R1M5-2) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     25-74: RKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKESKAILKARGLEEEQK → STMSPSENVSRAILKDSGSEEVEQA

Show »
Length:1,138
Mass (Da):128,353
Checksum:i4E6F124DD7049254
GO
Isoform 3 (identifier: Q9R1M5-3) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Show »
Length:1,111
Mass (Da):125,503
Checksum:i4016A5D67A1C01F4
GO
Isoform 4 (identifier: Q9R1M5-4) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Show »
Length:1,059
Mass (Da):119,687
Checksum:i97B55C54C16EA862
GO
Isoform 5 (identifier: Q9R1M5-5) [UniParc]FASTAAdd to basket
Also known as: E

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     181-196: Missing.

Show »
Length:1,095
Mass (Da):123,670
Checksum:iDDB219915C0F385C
GO
Isoform 6 (identifier: Q9R1M5-6) [UniParc]FASTAAdd to basket
Also known as: G

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     1065-1090: Missing.

Show »
Length:1,085
Mass (Da):122,857
Checksum:iFB8F4F35516DA926
GO
Isoform 7 (identifier: Q9R1M5-7) [UniParc]FASTAAdd to basket
Also known as: H

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     1037-1041: NNALG → WGHVS
     1042-1163: Missing.

Show »
Length:989
Mass (Da):111,920
Checksum:i125C9D27AB59123B
GO
Isoform 8 (identifier: Q9R1M5-8) [UniParc]FASTAAdd to basket
Also known as: F

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     780-1163: Missing.

Show »
Length:727
Mass (Da):83,001
Checksum:i0560C8AF9C2B88D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti567E → G in AAH53384 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti59E → D in strain: 129/Sv and CBA/J. 1 Publication1
Natural varianti94G → L in strain: 129/Sv; requires 2 nucleotide substitutions. 1 Publication1
Natural varianti139R → K in strain: 129/Sv and CBA/J. 1 Publication1
Natural varianti283V → G in strain: A/J. 1
Natural varianti308L → P in strain: 129/Sv and CBA/J. 1 Publication1
Natural varianti341S → T in strain: A/J. 1
Natural varianti1029H → Q in strain: 129/Sv and CBA/J. 1 Publication1
Natural varianti1055 – 1056SS → NN in strain: 129/Sv and CBA/J. 2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0247261 – 104Missing in isoform 4. 1 PublicationAdd BLAST104
Alternative sequenceiVSP_0247271 – 52Missing in isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8. 4 PublicationsAdd BLAST52
Alternative sequenceiVSP_02472825 – 74RKMTS…EEEQK → STMSPSENVSRAILKDSGSE EVEQA in isoform 2. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_024729181 – 196Missing in isoform 5. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_024730780 – 1163Missing in isoform 8. 1 PublicationAdd BLAST384
Alternative sequenceiVSP_0247311037 – 1041NNALG → WGHVS in isoform 7. 1 Publication5
Alternative sequenceiVSP_0247321042 – 1163Missing in isoform 7. 1 PublicationAdd BLAST122
Alternative sequenceiVSP_0247331065 – 1090Missing in isoform 6. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074018 mRNA. Translation: AAD51762.1.
AF143573
, AF143559, AF143560, AF143561, AF143562, AF143563, AF143564, AF143565, AF143566, AF143567, AF143568, AF143569, AF143570, AF143571, AF143572 Genomic DNA. Translation: AAF64393.1.
AY196361 mRNA. Translation: AAO52697.1.
AY196362 mRNA. Translation: AAO52698.1.
AY329484 mRNA. Translation: AAQ94607.1.
AY329485 mRNA. Translation: AAQ94608.1.
AY329486 mRNA. Translation: AAQ94609.1.
AY329487 mRNA. Translation: AAQ94610.1.
AY329488 mRNA. Translation: AAQ94611.1.
AY329489 mRNA. Translation: AAQ94612.1.
AY329490 mRNA. Translation: AAQ94613.1.
AY329491 mRNA. Translation: AAQ94614.1.
BC053384 mRNA. Translation: AAH53384.1.
AK135731 mRNA. Translation: BAE22633.1.
CCDSiCCDS20929.1. [Q9R1M5-5]
CCDS20930.1. [Q9R1M5-3]
PIRiA59000.
RefSeqiNP_001034232.1. NM_001039143.2. [Q9R1M5-5]
NP_001292786.1. NM_001305857.1. [Q9R1M5-6]
NP_035990.1. NM_011860.3. [Q9R1M5-3]
UniGeneiMm.333653.

Genome annotation databases

EnsembliENSMUST00000015866; ENSMUSP00000015866; ENSMUSG00000015721. [Q9R1M5-3]
ENSMUST00000086341; ENSMUSP00000083524; ENSMUSG00000015721. [Q9R1M5-5]
ENSMUST00000108441; ENSMUSP00000104080; ENSMUSG00000015721. [Q9R1M5-6]
ENSMUST00000133237; ENSMUSP00000122007; ENSMUSG00000015721. [Q9R1M5-7]
ENSMUST00000139661; ENSMUSP00000118638; ENSMUSG00000015721. [Q9R1M5-8]
GeneIDi23968.
KEGGimmu:23968.
UCSCiuc009foe.2. mouse. [Q9R1M5-1]
uc009fof.2. mouse. [Q9R1M5-5]
uc012fev.2. mouse. [Q9R1M5-6]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074018 mRNA. Translation: AAD51762.1.
AF143573
, AF143559, AF143560, AF143561, AF143562, AF143563, AF143564, AF143565, AF143566, AF143567, AF143568, AF143569, AF143570, AF143571, AF143572 Genomic DNA. Translation: AAF64393.1.
AY196361 mRNA. Translation: AAO52697.1.
AY196362 mRNA. Translation: AAO52698.1.
AY329484 mRNA. Translation: AAQ94607.1.
AY329485 mRNA. Translation: AAQ94608.1.
AY329486 mRNA. Translation: AAQ94609.1.
AY329487 mRNA. Translation: AAQ94610.1.
AY329488 mRNA. Translation: AAQ94611.1.
AY329489 mRNA. Translation: AAQ94612.1.
AY329490 mRNA. Translation: AAQ94613.1.
AY329491 mRNA. Translation: AAQ94614.1.
BC053384 mRNA. Translation: AAH53384.1.
AK135731 mRNA. Translation: BAE22633.1.
CCDSiCCDS20929.1. [Q9R1M5-5]
CCDS20930.1. [Q9R1M5-3]
PIRiA59000.
RefSeqiNP_001034232.1. NM_001039143.2. [Q9R1M5-5]
NP_001292786.1. NM_001305857.1. [Q9R1M5-6]
NP_035990.1. NM_011860.3. [Q9R1M5-3]
UniGeneiMm.333653.

3D structure databases

ProteinModelPortaliQ9R1M5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204829. 1 interactor.
IntActiQ9R1M5. 3 interactors.
STRINGi10090.ENSMUSP00000015866.

PTM databases

iPTMnetiQ9R1M5.
PhosphoSitePlusiQ9R1M5.

Proteomic databases

PaxDbiQ9R1M5.
PRIDEiQ9R1M5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015866; ENSMUSP00000015866; ENSMUSG00000015721. [Q9R1M5-3]
ENSMUST00000086341; ENSMUSP00000083524; ENSMUSG00000015721. [Q9R1M5-5]
ENSMUST00000108441; ENSMUSP00000104080; ENSMUSG00000015721. [Q9R1M5-6]
ENSMUST00000133237; ENSMUSP00000122007; ENSMUSG00000015721. [Q9R1M5-7]
ENSMUST00000139661; ENSMUSP00000118638; ENSMUSG00000015721. [Q9R1M5-8]
GeneIDi23968.
KEGGimmu:23968.
UCSCiuc009foe.2. mouse. [Q9R1M5-1]
uc009fof.2. mouse. [Q9R1M5-5]
uc012fev.2. mouse. [Q9R1M5-6]

Organism-specific databases

CTDi126206.
MGIiMGI:1345193. Nlrp5.

Phylogenomic databases

eggNOGiENOG410IIY8. Eukaryota.
ENOG4111BJJ. LUCA.
GeneTreeiENSGT00860000133669.
HOVERGENiHBG063656.
InParanoidiQ9R1M5.
OMAiMTSPEND.
OrthoDBiEOG091G01CG.
PhylomeDBiQ9R1M5.

Miscellaneous databases

PROiQ9R1M5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015721.
CleanExiMM_NLRP5.
GenevisibleiQ9R1M5. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 4 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNALP5_MOUSE
AccessioniPrimary (citable) accession number: Q9R1M5
Secondary accession number(s): Q3UXD0
, Q53ZD5, Q6VSF9, Q6VSG0, Q6VSG1, Q6VSG2, Q6VSG3, Q6VSG4, Q6VSG5, Q6XZF2, Q7TPU9, Q9JLR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.