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Q9R1L5

- MAST1_MOUSE

UniProt

Q9R1L5 - MAST1_MOUSE

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Protein

Microtubule-associated serine/threonine-protein kinase 1

Gene

Mast1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.1 Publication1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei404 – 4041ATPBy similarityPROSITE-ProRule annotation
Active sitei498 – 4981Proton acceptorBy similarityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi381 – 3899ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cytoskeleton organization Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated serine/threonine-protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
Syntrophin-associated serine/threonine-protein kinase
Gene namesi
Name:Mast1Imported
Synonyms:Kiaa0973, SastImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1861901. Mast1.

Subcellular locationi

Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Cytoplasmcytoskeleton 1 Publication
Note: Colocalizes with syntrophins at the cell membrane.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-KW
  3. membrane Source: UniProtKB
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15701570Microtubule-associated serine/threonine-protein kinase 1PRO_0000086310Add
BLAST

Proteomic databases

MaxQBiQ9R1L5.
PaxDbiQ9R1L5.
PRIDEiQ9R1L5.

PTM databases

PhosphoSiteiQ9R1L5.

Expressioni

Tissue specificityi

Expressed in the vascular endothelium and in proximity to neuronal nuclei throughout the cortex and cerebellum. Also detected in ependymal cells, the choroid plexus, in developing spermatid acrosomes and in both the cell bodies and axonal processes of motor neurons. Observed as punctate clusters in the processes of interneurons and along the cell body periphery.1 Publication

Gene expression databases

BgeeiQ9R1L5.
CleanExiMM_MAST1.
ExpressionAtlasiQ9R1L5. baseline and differential.
GenevestigatoriQ9R1L5.

Interactioni

Subunit structurei

Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTENP604843EBI-491771,EBI-696162From a different organism.

Protein-protein interaction databases

BioGridi208038. 1 interaction.
IntActiQ9R1L5. 3 interactions.
MINTiMINT-204974.
STRINGi10090.ENSMUSP00000105363.

Structurei

3D structure databases

ProteinModelPortaliQ9R1L5.
SMRiQ9R1L5. Positions 187-287, 314-703, 967-1059.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini375 – 648274Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini649 – 72072AGC-kinase C-terminalAdd
BLAST
Domaini968 – 105689PDZPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000046662.
HOVERGENiHBG052414.
InParanoidiQ9R1L5.
KOiK08789.
OMAiGCTRHQS.
OrthoDBiEOG7PK8ZC.
PhylomeDBiQ9R1L5.
TreeFamiTF313149.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028777. MAST1.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24356:SF150. PTHR24356:SF150. 1 hit.
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1L5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH
60 70 80 90 100
SPLPGHLGSS PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG
110 120 130 140 150
TNTPSSTVSS SCSSQERLHQ LPYQPTVDEL HFLSKHFGST ESITDEDGGR
160 170 180 190 200
RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH VYKERFPKAT AQMEEKLRDF
210 220 230 240 250
ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI TTVYFYELQE
260 270 280 290 300
NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY
310 320 330 340 350
HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN
360 370 380 390 400
TPEQDDTSEG RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ
410 420 430 440 450
RFAMKKINKQ NLILRNQIQQ AFVERDILTF AENPFVVGMF CSFETRRHLC
460 470 480 490 500
MVMEYVEGGD CATLLKNIGA LPVEMARMYF AETVLALEYL HNYGIVHRDL
510 520 530 540 550
KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD AREFLDKQVC
560 570 580 590 600
GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ
610 620 630 640 650
VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR
660 670 680 690 700
DLDWTGLLRQ KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP
710 720 730 740 750
VEIRQFSSCS PRFSKVYSSM EQLSQHEPKT PVSASGASKR DPSAKGPEEK
760 770 780 790 800
VAGKREGLGG LTLREKTWRG GSPEIKRFSA SEASFLEGEA SPPLGARRRF
810 820 830 840 850
SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP KEAVQGEGTS
860 870 880 890 900
TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV
910 920 930 940 950
EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS
960 970 980 990 1000
RDSSPSRDYS PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH
1010 1020 1030 1040 1050
IVWHVEEGGP AQEAGLCAGD LITHVNGEPV HGMVHPEVVE LILKSGNKVA
1060 1070 1080 1090 1100
VTTTPFENTS IRIGPARRSS YKAKMARRNK RPSAKDGQES KKRSSLFRKI
1110 1120 1130 1140 1150
TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT HSYRSTPDSA
1160 1170 1180 1190 1200
YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS
1210 1220 1230 1240 1250
AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP
1260 1270 1280 1290 1300
RPKSAEPPRS PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF
1310 1320 1330 1340 1350
HGELALHSLA ESDGETPPIE GPGATRQVAV RRLGRQESPL SLGADPLLPD
1360 1370 1380 1390 1400
GVQRPMASSK EDSAGGTEAC TPPRATTPGS RTLERDSGCT RHQSVQTEDG
1410 1420 1430 1440 1450
PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE PARPGVKTQA
1460 1470 1480 1490 1500
PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA
1510 1520 1530 1540 1550
SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE
1560 1570
AVTPAGLTKT GAPSPASLGP
Length:1,570
Mass (Da):170,997
Last modified:September 11, 2007 - v3
Checksum:iA8F9C868CF241486
GO

Sequence cautioni

The sequence AAD50548.1 differs from that shown. Reason: Frameshift at several positions. Curated
The sequence BAC65693.3 differs from that shown. Reason: The sequence differs from that shown because it seems to be derived from a pre-mRNA.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581R → P in AAD50548. (PubMed:10404183)Curated
Sequence conflicti197 – 1982LR → P in AAD50548. (PubMed:10404183)Curated
Sequence conflicti647 – 6471S → T in AAD50548. (PubMed:10404183)Curated
Sequence conflicti672 – 6721D → E in AAD50548. (PubMed:10404183)Curated
Sequence conflicti1190 – 11901H → L in AAD50548. (PubMed:10404183)Curated
Sequence conflicti1219 – 125032SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548. (PubMed:10404183)CuratedAdd
BLAST
Sequence conflicti1454 – 14541L → V in AAD50548. (PubMed:10404183)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077818 mRNA. Translation: AAD50548.1. Frameshift.
BC054524 mRNA. Translation: AAH54524.1.
AK122411 Transcribed RNA. Translation: BAC65693.3. Sequence problems.
CCDSiCCDS40415.1.
RefSeqiNP_064329.2. NM_019945.2.
UniGeneiMm.154614.

Genome annotation databases

EnsembliENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693.
GeneIDi56527.
KEGGimmu:56527.
UCSCiuc009mof.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077818 mRNA. Translation: AAD50548.1 . Frameshift.
BC054524 mRNA. Translation: AAH54524.1 .
AK122411 Transcribed RNA. Translation: BAC65693.3 . Sequence problems.
CCDSi CCDS40415.1.
RefSeqi NP_064329.2. NM_019945.2.
UniGenei Mm.154614.

3D structure databases

ProteinModelPortali Q9R1L5.
SMRi Q9R1L5. Positions 187-287, 314-703, 967-1059.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208038. 1 interaction.
IntActi Q9R1L5. 3 interactions.
MINTi MINT-204974.
STRINGi 10090.ENSMUSP00000105363.

PTM databases

PhosphoSitei Q9R1L5.

Proteomic databases

MaxQBi Q9R1L5.
PaxDbi Q9R1L5.
PRIDEi Q9R1L5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000109741 ; ENSMUSP00000105363 ; ENSMUSG00000053693 .
GeneIDi 56527.
KEGGi mmu:56527.
UCSCi uc009mof.1. mouse.

Organism-specific databases

CTDi 22983.
MGIi MGI:1861901. Mast1.
Rougei Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063286.
HOGENOMi HOG000046662.
HOVERGENi HBG052414.
InParanoidi Q9R1L5.
KOi K08789.
OMAi GCTRHQS.
OrthoDBi EOG7PK8ZC.
PhylomeDBi Q9R1L5.
TreeFami TF313149.

Miscellaneous databases

NextBioi 312870.
PROi Q9R1L5.
SOURCEi Search...

Gene expression databases

Bgeei Q9R1L5.
CleanExi MM_MAST1.
ExpressionAtlasi Q9R1L5. baseline and differential.
Genevestigatori Q9R1L5.

Family and domain databases

Gene3Di 1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028777. MAST1.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24356:SF150. PTHR24356:SF150. 1 hit.
Pfami PF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases."
    Lumeng C., Phelps S., Crawford G.E., Walden P.D., Barald K., Chamberlain J.S.
    Nat. Neurosci. 2:611-617(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SNTB2.
    Strain: C57BL/10Imported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6Imported.
    Tissue: BrainImported.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 337-1570.
    Tissue: BrainImported.
  4. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiMAST1_MOUSE
AccessioniPrimary (citable) accession number: Q9R1L5
Secondary accession number(s): Q7TQG9, Q80TN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: September 11, 2007
Last modified: November 26, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3