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Q9R1L5 (MAST1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated serine/threonine-protein kinase 1

EC=2.7.11.1
Alternative name(s):
Syntrophin-associated serine/threonine-protein kinase
Gene names
Name:Mast1
Synonyms:Kiaa0973, Sast
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1570 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9BXM7

Cofactor

Magnesium By similarity. UniProtKB Q9BXM7

Subunit structure

Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2. Ref.1

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Note: Colocalizes with syntrophins at the cell membrane. Ref.1

Tissue specificity

Expressed in the vascular endothelium and in proximity to neuronal nuclei throughout the cortex and cerebellum. Also detected in ependymal cells, the choroid plexus, in developing spermatid acrosomes and in both the cell bodies and axonal processes of motor neurons. Observed as punctate clusters in the processes of interneurons and along the cell body periphery. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PDZ (DHR) domain.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAD50548.1 differs from that shown. Reason: Frameshift at several positions.

The sequence BAC65693.3 differs from that shown. Reason: The sequence differs from that shown because it seems to be derived from a pre-mRNA.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PTENP604843EBI-491771,EBI-696162From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15701570Microtubule-associated serine/threonine-protein kinase 1
PRO_0000086310

Regions

Domain375 – 648274Protein kinase
Domain649 – 72072AGC-kinase C-terminal
Domain968 – 105689PDZ
Nucleotide binding381 – 3899ATP By similarity UniProtKB Q9BXM7

Sites

Active site4981Proton acceptor By similarity UniProtKB Q9BXM7
Binding site4041ATP By similarity UniProtKB Q9BXM7

Experimental info

Sequence conflict1581R → P in AAD50548. Ref.1
Sequence conflict197 – 1982LR → P in AAD50548. Ref.1
Sequence conflict6471S → T in AAD50548. Ref.1
Sequence conflict6721D → E in AAD50548. Ref.1
Sequence conflict11901H → L in AAD50548. Ref.1
Sequence conflict1219 – 125032SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548. Ref.1
Sequence conflict14541L → V in AAD50548. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9R1L5 [UniParc].

Last modified September 11, 2007. Version 3.
Checksum: A8F9C868CF241486

FASTA1,570170,997
        10         20         30         40         50         60 
MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH SPLPGHLGSS 

        70         80         90        100        110        120 
PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG TNTPSSTVSS SCSSQERLHQ 

       130        140        150        160        170        180 
LPYQPTVDEL HFLSKHFGST ESITDEDGGR RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH 

       190        200        210        220        230        240 
VYKERFPKAT AQMEEKLRDF ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI 

       250        260        270        280        290        300 
TTVYFYELQE NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY 

       310        320        330        340        350        360 
HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN TPEQDDTSEG 

       370        380        390        400        410        420 
RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ RFAMKKINKQ NLILRNQIQQ 

       430        440        450        460        470        480 
AFVERDILTF AENPFVVGMF CSFETRRHLC MVMEYVEGGD CATLLKNIGA LPVEMARMYF 

       490        500        510        520        530        540 
AETVLALEYL HNYGIVHRDL KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD 

       550        560        570        580        590        600 
AREFLDKQVC GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ 

       610        620        630        640        650        660 
VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR DLDWTGLLRQ 

       670        680        690        700        710        720 
KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP VEIRQFSSCS PRFSKVYSSM 

       730        740        750        760        770        780 
EQLSQHEPKT PVSASGASKR DPSAKGPEEK VAGKREGLGG LTLREKTWRG GSPEIKRFSA 

       790        800        810        820        830        840 
SEASFLEGEA SPPLGARRRF SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP 

       850        860        870        880        890        900 
KEAVQGEGTS TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV 

       910        920        930        940        950        960 
EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS RDSSPSRDYS 

       970        980        990       1000       1010       1020 
PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH IVWHVEEGGP AQEAGLCAGD 

      1030       1040       1050       1060       1070       1080 
LITHVNGEPV HGMVHPEVVE LILKSGNKVA VTTTPFENTS IRIGPARRSS YKAKMARRNK 

      1090       1100       1110       1120       1130       1140 
RPSAKDGQES KKRSSLFRKI TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT 

      1150       1160       1170       1180       1190       1200 
HSYRSTPDSA YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS 

      1210       1220       1230       1240       1250       1260 
AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP RPKSAEPPRS 

      1270       1280       1290       1300       1310       1320 
PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF HGELALHSLA ESDGETPPIE 

      1330       1340       1350       1360       1370       1380 
GPGATRQVAV RRLGRQESPL SLGADPLLPD GVQRPMASSK EDSAGGTEAC TPPRATTPGS 

      1390       1400       1410       1420       1430       1440 
RTLERDSGCT RHQSVQTEDG PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE 

      1450       1460       1470       1480       1490       1500 
PARPGVKTQA PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA 

      1510       1520       1530       1540       1550       1560 
SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE AVTPAGLTKT 

      1570 
GAPSPASLGP 

« Hide

References

« Hide 'large scale' references
[1]"Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases."
Lumeng C., Phelps S., Crawford G.E., Walden P.D., Barald K., Chamberlain J.S.
Nat. Neurosci. 2:611-617(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SNTB2.
Strain: C57BL/10.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 337-1570.
Tissue: Brain.
[4]Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[5]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF077818 mRNA. Translation: AAD50548.1. Frameshift.
BC054524 mRNA. Translation: AAH54524.1.
AK122411 Transcribed RNA. Translation: BAC65693.3. Sequence problems.
RefSeqNP_064329.2. NM_019945.2.
UniGeneMm.154614.

3D structure databases

ProteinModelPortalQ9R1L5.
SMRQ9R1L5. Positions 185-678, 967-1059.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208038. 1 interaction.
IntActQ9R1L5. 3 interactions.
MINTMINT-204974.
STRING10090.ENSMUSP00000105363.

PTM databases

PhosphoSiteQ9R1L5.

Proteomic databases

PaxDbQ9R1L5.
PRIDEQ9R1L5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693.
GeneID56527.
KEGGmmu:56527.
UCSCuc009mof.1. mouse.

Organism-specific databases

CTD22983.
MGIMGI:1861901. Mast1.
RougeSearch...

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000063286.
HOGENOMHOG000046662.
HOVERGENHBG052414.
InParanoidQ9R1L5.
KOK08789.
OMAGCTRHQS.
OrthoDBEOG7PK8ZC.
PhylomeDBQ9R1L5.
TreeFamTF313149.

Gene expression databases

BgeeQ9R1L5.
CleanExMM_MAST1.
GenevestigatorQ9R1L5.

Family and domain databases

Gene3D1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028777. MAST1.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24356:SF118. PTHR24356:SF118. 1 hit.
PfamPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio312870.
PROQ9R1L5.
SOURCESearch...

Entry information

Entry nameMAST1_MOUSE
AccessionPrimary (citable) accession number: Q9R1L5
Secondary accession number(s): Q7TQG9, Q80TN0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot