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Protein

Microtubule-associated serine/threonine-protein kinase 1

Gene

Mast1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.1 Publication1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei404ATPPROSITE-ProRule annotationBy similarity1
Active sitei498Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi381 – 389ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated serine/threonine-protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
Syntrophin-associated serine/threonine-protein kinase
Gene namesi
Name:Mast1Imported
Synonyms:Kiaa0973, SastImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1861901 Mast1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863101 – 1570Microtubule-associated serine/threonine-protein kinase 1Add BLAST1570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei167PhosphoserineCombined sources1
Modified residuei346PhosphoserineBy similarity1
Modified residuei351PhosphothreonineBy similarity1
Modified residuei689PhosphoserineBy similarity1
Modified residuei895PhosphoserineCombined sources1
Modified residuei954PhosphoserineBy similarity1
Modified residuei1414PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9R1L5
PaxDbiQ9R1L5
PeptideAtlasiQ9R1L5
PRIDEiQ9R1L5

PTM databases

iPTMnetiQ9R1L5
PhosphoSitePlusiQ9R1L5

Expressioni

Tissue specificityi

Expressed in the vascular endothelium and in proximity to neuronal nuclei throughout the cortex and cerebellum. Also detected in ependymal cells, the choroid plexus, in developing spermatid acrosomes and in both the cell bodies and axonal processes of motor neurons. Observed as punctate clusters in the processes of interneurons and along the cell body periphery.1 Publication

Gene expression databases

BgeeiENSMUSG00000053693
CleanExiMM_MAST1
ExpressionAtlasiQ9R1L5 baseline and differential
GenevisibleiQ9R1L5 MM

Interactioni

Subunit structurei

Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTENP604843EBI-491771,EBI-696162From Homo sapiens.

Protein-protein interaction databases

BioGridi208038, 1 interactor
IntActiQ9R1L5, 3 interactors
MINTiQ9R1L5
STRINGi10090.ENSMUSP00000105363

Structurei

3D structure databases

ProteinModelPortaliQ9R1L5
SMRiQ9R1L5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini375 – 648Protein kinasePROSITE-ProRule annotationAdd BLAST274
Domaini649 – 720AGC-kinase C-terminalAdd BLAST72
Domaini968 – 1056PDZPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0606 Eukaryota
ENOG410XPWX LUCA
GeneTreeiENSGT00530000063286
HOGENOMiHOG000046662
HOVERGENiHBG052414
InParanoidiQ9R1L5
KOiK08789
OMAiRPGDLCP
OrthoDBiEOG091G00IH
PhylomeDBiQ9R1L5
TreeFamiTF313149

Family and domain databases

CDDicd05609 STKc_MAST, 1 hit
Gene3Di1.20.1480.20, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011009 Kinase-like_dom_sf
IPR015022 MA_Ser/Thr_Kinase_dom
IPR037711 MAST
IPR028777 MAST1
IPR023142 MAST_pre-PK_dom_sf
IPR001478 PDZ
IPR036034 PDZ_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR24356:SF150 PTHR24356:SF150, 1 hit
PfamiView protein in Pfam
PF08926 DUF1908, 1 hit
PF00595 PDZ, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF140482 SSF140482, 1 hit
SSF50156 SSF50156, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50106 PDZ, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9R1L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH
60 70 80 90 100
SPLPGHLGSS PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG
110 120 130 140 150
TNTPSSTVSS SCSSQERLHQ LPYQPTVDEL HFLSKHFGST ESITDEDGGR
160 170 180 190 200
RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH VYKERFPKAT AQMEEKLRDF
210 220 230 240 250
ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI TTVYFYELQE
260 270 280 290 300
NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY
310 320 330 340 350
HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN
360 370 380 390 400
TPEQDDTSEG RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ
410 420 430 440 450
RFAMKKINKQ NLILRNQIQQ AFVERDILTF AENPFVVGMF CSFETRRHLC
460 470 480 490 500
MVMEYVEGGD CATLLKNIGA LPVEMARMYF AETVLALEYL HNYGIVHRDL
510 520 530 540 550
KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD AREFLDKQVC
560 570 580 590 600
GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ
610 620 630 640 650
VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR
660 670 680 690 700
DLDWTGLLRQ KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP
710 720 730 740 750
VEIRQFSSCS PRFSKVYSSM EQLSQHEPKT PVSASGASKR DPSAKGPEEK
760 770 780 790 800
VAGKREGLGG LTLREKTWRG GSPEIKRFSA SEASFLEGEA SPPLGARRRF
810 820 830 840 850
SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP KEAVQGEGTS
860 870 880 890 900
TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV
910 920 930 940 950
EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS
960 970 980 990 1000
RDSSPSRDYS PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH
1010 1020 1030 1040 1050
IVWHVEEGGP AQEAGLCAGD LITHVNGEPV HGMVHPEVVE LILKSGNKVA
1060 1070 1080 1090 1100
VTTTPFENTS IRIGPARRSS YKAKMARRNK RPSAKDGQES KKRSSLFRKI
1110 1120 1130 1140 1150
TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT HSYRSTPDSA
1160 1170 1180 1190 1200
YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS
1210 1220 1230 1240 1250
AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP
1260 1270 1280 1290 1300
RPKSAEPPRS PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF
1310 1320 1330 1340 1350
HGELALHSLA ESDGETPPIE GPGATRQVAV RRLGRQESPL SLGADPLLPD
1360 1370 1380 1390 1400
GVQRPMASSK EDSAGGTEAC TPPRATTPGS RTLERDSGCT RHQSVQTEDG
1410 1420 1430 1440 1450
PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE PARPGVKTQA
1460 1470 1480 1490 1500
PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA
1510 1520 1530 1540 1550
SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE
1560 1570
AVTPAGLTKT GAPSPASLGP
Length:1,570
Mass (Da):170,997
Last modified:September 11, 2007 - v3
Checksum:iA8F9C868CF241486
GO

Sequence cautioni

The sequence AAD50548 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence BAC65693 differs from that shown. The sequence differs from that shown because it seems to be derived from a pre-mRNA.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158R → P in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti197 – 198LR → P in AAD50548 (PubMed:10404183).Curated2
Sequence conflicti647S → T in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti672D → E in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti1190H → L in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti1219 – 1250SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548 (PubMed:10404183).CuratedAdd BLAST32
Sequence conflicti1454L → V in AAD50548 (PubMed:10404183).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077818 mRNA Translation: AAD50548.1 Frameshift.
BC054524 mRNA Translation: AAH54524.1
AK122411 Transcribed RNA Translation: BAC65693.3 Sequence problems.
CCDSiCCDS40415.1
RefSeqiNP_064329.2, NM_019945.2
UniGeneiMm.154614

Genome annotation databases

EnsembliENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693
GeneIDi56527
KEGGimmu:56527
UCSCiuc009mof.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMAST1_MOUSE
AccessioniPrimary (citable) accession number: Q9R1L5
Secondary accession number(s): Q7TQG9, Q80TN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: September 11, 2007
Last modified: May 23, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

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