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Protein

Microtubule-associated serine/threonine-protein kinase 1

Gene

Mast1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.1 Publication1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei404ATPPROSITE-ProRule annotationBy similarity1
Active sitei498Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi381 – 389ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated serine/threonine-protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
Syntrophin-associated serine/threonine-protein kinase
Gene namesi
Name:Mast1Imported
Synonyms:Kiaa0973, SastImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1861901. Mast1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • intracellular Source: GO_Central
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863101 – 1570Microtubule-associated serine/threonine-protein kinase 1Add BLAST1570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei167PhosphoserineCombined sources1
Modified residuei346PhosphoserineBy similarity1
Modified residuei351PhosphothreonineBy similarity1
Modified residuei689PhosphoserineBy similarity1
Modified residuei895PhosphoserineCombined sources1
Modified residuei954PhosphoserineBy similarity1
Modified residuei1414PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9R1L5.
PaxDbiQ9R1L5.
PeptideAtlasiQ9R1L5.
PRIDEiQ9R1L5.

PTM databases

iPTMnetiQ9R1L5.
PhosphoSitePlusiQ9R1L5.

Expressioni

Tissue specificityi

Expressed in the vascular endothelium and in proximity to neuronal nuclei throughout the cortex and cerebellum. Also detected in ependymal cells, the choroid plexus, in developing spermatid acrosomes and in both the cell bodies and axonal processes of motor neurons. Observed as punctate clusters in the processes of interneurons and along the cell body periphery.1 Publication

Gene expression databases

BgeeiENSMUSG00000053693.
CleanExiMM_MAST1.
ExpressionAtlasiQ9R1L5. baseline and differential.
GenevisibleiQ9R1L5. MM.

Interactioni

Subunit structurei

Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTENP604843EBI-491771,EBI-696162From a different organism.

Protein-protein interaction databases

BioGridi208038. 1 interactor.
IntActiQ9R1L5. 3 interactors.
MINTiMINT-204974.
STRINGi10090.ENSMUSP00000105363.

Structurei

3D structure databases

ProteinModelPortaliQ9R1L5.
SMRiQ9R1L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini375 – 648Protein kinasePROSITE-ProRule annotationAdd BLAST274
Domaini649 – 720AGC-kinase C-terminalAdd BLAST72
Domaini968 – 1056PDZPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0606. Eukaryota.
ENOG410XPWX. LUCA.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000046662.
HOVERGENiHBG052414.
InParanoidiQ9R1L5.
KOiK08789.
OMAiGCTRHQS.
OrthoDBiEOG091G00IH.
PhylomeDBiQ9R1L5.
TreeFamiTF313149.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028777. MAST1.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24356:SF150. PTHR24356:SF150. 2 hits.
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH
60 70 80 90 100
SPLPGHLGSS PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG
110 120 130 140 150
TNTPSSTVSS SCSSQERLHQ LPYQPTVDEL HFLSKHFGST ESITDEDGGR
160 170 180 190 200
RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH VYKERFPKAT AQMEEKLRDF
210 220 230 240 250
ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI TTVYFYELQE
260 270 280 290 300
NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY
310 320 330 340 350
HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN
360 370 380 390 400
TPEQDDTSEG RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ
410 420 430 440 450
RFAMKKINKQ NLILRNQIQQ AFVERDILTF AENPFVVGMF CSFETRRHLC
460 470 480 490 500
MVMEYVEGGD CATLLKNIGA LPVEMARMYF AETVLALEYL HNYGIVHRDL
510 520 530 540 550
KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD AREFLDKQVC
560 570 580 590 600
GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ
610 620 630 640 650
VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR
660 670 680 690 700
DLDWTGLLRQ KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP
710 720 730 740 750
VEIRQFSSCS PRFSKVYSSM EQLSQHEPKT PVSASGASKR DPSAKGPEEK
760 770 780 790 800
VAGKREGLGG LTLREKTWRG GSPEIKRFSA SEASFLEGEA SPPLGARRRF
810 820 830 840 850
SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP KEAVQGEGTS
860 870 880 890 900
TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV
910 920 930 940 950
EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS
960 970 980 990 1000
RDSSPSRDYS PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH
1010 1020 1030 1040 1050
IVWHVEEGGP AQEAGLCAGD LITHVNGEPV HGMVHPEVVE LILKSGNKVA
1060 1070 1080 1090 1100
VTTTPFENTS IRIGPARRSS YKAKMARRNK RPSAKDGQES KKRSSLFRKI
1110 1120 1130 1140 1150
TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT HSYRSTPDSA
1160 1170 1180 1190 1200
YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS
1210 1220 1230 1240 1250
AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP
1260 1270 1280 1290 1300
RPKSAEPPRS PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF
1310 1320 1330 1340 1350
HGELALHSLA ESDGETPPIE GPGATRQVAV RRLGRQESPL SLGADPLLPD
1360 1370 1380 1390 1400
GVQRPMASSK EDSAGGTEAC TPPRATTPGS RTLERDSGCT RHQSVQTEDG
1410 1420 1430 1440 1450
PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE PARPGVKTQA
1460 1470 1480 1490 1500
PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA
1510 1520 1530 1540 1550
SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE
1560 1570
AVTPAGLTKT GAPSPASLGP
Length:1,570
Mass (Da):170,997
Last modified:September 11, 2007 - v3
Checksum:iA8F9C868CF241486
GO

Sequence cautioni

The sequence AAD50548 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence BAC65693 differs from that shown. The sequence differs from that shown because it seems to be derived from a pre-mRNA.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158R → P in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti197 – 198LR → P in AAD50548 (PubMed:10404183).Curated2
Sequence conflicti647S → T in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti672D → E in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti1190H → L in AAD50548 (PubMed:10404183).Curated1
Sequence conflicti1219 – 1250SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548 (PubMed:10404183).CuratedAdd BLAST32
Sequence conflicti1454L → V in AAD50548 (PubMed:10404183).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077818 mRNA. Translation: AAD50548.1. Frameshift.
BC054524 mRNA. Translation: AAH54524.1.
AK122411 Transcribed RNA. Translation: BAC65693.3. Sequence problems.
CCDSiCCDS40415.1.
RefSeqiNP_064329.2. NM_019945.2.
UniGeneiMm.154614.

Genome annotation databases

EnsembliENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693.
GeneIDi56527.
KEGGimmu:56527.
UCSCiuc009mof.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077818 mRNA. Translation: AAD50548.1. Frameshift.
BC054524 mRNA. Translation: AAH54524.1.
AK122411 Transcribed RNA. Translation: BAC65693.3. Sequence problems.
CCDSiCCDS40415.1.
RefSeqiNP_064329.2. NM_019945.2.
UniGeneiMm.154614.

3D structure databases

ProteinModelPortaliQ9R1L5.
SMRiQ9R1L5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208038. 1 interactor.
IntActiQ9R1L5. 3 interactors.
MINTiMINT-204974.
STRINGi10090.ENSMUSP00000105363.

PTM databases

iPTMnetiQ9R1L5.
PhosphoSitePlusiQ9R1L5.

Proteomic databases

MaxQBiQ9R1L5.
PaxDbiQ9R1L5.
PeptideAtlasiQ9R1L5.
PRIDEiQ9R1L5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693.
GeneIDi56527.
KEGGimmu:56527.
UCSCiuc009mof.1. mouse.

Organism-specific databases

CTDi22983.
MGIiMGI:1861901. Mast1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0606. Eukaryota.
ENOG410XPWX. LUCA.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000046662.
HOVERGENiHBG052414.
InParanoidiQ9R1L5.
KOiK08789.
OMAiGCTRHQS.
OrthoDBiEOG091G00IH.
PhylomeDBiQ9R1L5.
TreeFamiTF313149.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Miscellaneous databases

PROiQ9R1L5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053693.
CleanExiMM_MAST1.
ExpressionAtlasiQ9R1L5. baseline and differential.
GenevisibleiQ9R1L5. MM.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028777. MAST1.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24356:SF150. PTHR24356:SF150. 2 hits.
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAST1_MOUSE
AccessioniPrimary (citable) accession number: Q9R1L5
Secondary accession number(s): Q7TQG9, Q80TN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: September 11, 2007
Last modified: November 2, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.