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Q9R1L5

- MAST1_MOUSE

UniProt

Q9R1L5 - MAST1_MOUSE

Protein

Microtubule-associated serine/threonine-protein kinase 1

Gene

Mast1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.1 Publication1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei404 – 4041ATPBy similarityPROSITE-ProRule annotation
    Active sitei498 – 4981Proton acceptorBy similarityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi381 – 3899ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cytoskeleton organization Source: UniProtKB
    2. intracellular signal transduction Source: UniProtKB
    3. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated serine/threonine-protein kinase 1 (EC:2.7.11.1)
    Alternative name(s):
    Syntrophin-associated serine/threonine-protein kinase
    Gene namesi
    Name:Mast1Imported
    Synonyms:Kiaa0973, SastImported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1861901. Mast1.

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: Colocalizes with syntrophins at the cell membrane.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. cytoskeleton Source: UniProtKB-SubCell
    3. membrane Source: UniProtKB
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15701570Microtubule-associated serine/threonine-protein kinase 1PRO_0000086310Add
    BLAST

    Proteomic databases

    PaxDbiQ9R1L5.
    PRIDEiQ9R1L5.

    PTM databases

    PhosphoSiteiQ9R1L5.

    Expressioni

    Tissue specificityi

    Expressed in the vascular endothelium and in proximity to neuronal nuclei throughout the cortex and cerebellum. Also detected in ependymal cells, the choroid plexus, in developing spermatid acrosomes and in both the cell bodies and axonal processes of motor neurons. Observed as punctate clusters in the processes of interneurons and along the cell body periphery.1 Publication

    Gene expression databases

    BgeeiQ9R1L5.
    CleanExiMM_MAST1.
    GenevestigatoriQ9R1L5.

    Interactioni

    Subunit structurei

    Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PTENP604843EBI-491771,EBI-696162From a different organism.

    Protein-protein interaction databases

    BioGridi208038. 1 interaction.
    IntActiQ9R1L5. 3 interactions.
    MINTiMINT-204974.
    STRINGi10090.ENSMUSP00000105363.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R1L5.
    SMRiQ9R1L5. Positions 187-691, 967-1059.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini375 – 648274Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini649 – 72072AGC-kinase C-terminalAdd
    BLAST
    Domaini968 – 105689PDZPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063286.
    HOGENOMiHOG000046662.
    HOVERGENiHBG052414.
    InParanoidiQ9R1L5.
    KOiK08789.
    OMAiGCTRHQS.
    OrthoDBiEOG7PK8ZC.
    PhylomeDBiQ9R1L5.
    TreeFamiTF313149.

    Family and domain databases

    Gene3Di1.20.1480.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR015022. MA_Ser/Thr_Kinase_dom.
    IPR028777. MAST1.
    IPR023142. MAST_pre-PK_dom.
    IPR001478. PDZ.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24356:SF150. PTHR24356:SF150. 1 hit.
    PfamiPF08926. DUF1908. 1 hit.
    PF00595. PDZ. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF140482. SSF140482. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R1L5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH     50
    SPLPGHLGSS PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG 100
    TNTPSSTVSS SCSSQERLHQ LPYQPTVDEL HFLSKHFGST ESITDEDGGR 150
    RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH VYKERFPKAT AQMEEKLRDF 200
    ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI TTVYFYELQE 250
    NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY 300
    HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN 350
    TPEQDDTSEG RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ 400
    RFAMKKINKQ NLILRNQIQQ AFVERDILTF AENPFVVGMF CSFETRRHLC 450
    MVMEYVEGGD CATLLKNIGA LPVEMARMYF AETVLALEYL HNYGIVHRDL 500
    KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD AREFLDKQVC 550
    GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ 600
    VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR 650
    DLDWTGLLRQ KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP 700
    VEIRQFSSCS PRFSKVYSSM EQLSQHEPKT PVSASGASKR DPSAKGPEEK 750
    VAGKREGLGG LTLREKTWRG GSPEIKRFSA SEASFLEGEA SPPLGARRRF 800
    SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP KEAVQGEGTS 850
    TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV 900
    EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS 950
    RDSSPSRDYS PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH 1000
    IVWHVEEGGP AQEAGLCAGD LITHVNGEPV HGMVHPEVVE LILKSGNKVA 1050
    VTTTPFENTS IRIGPARRSS YKAKMARRNK RPSAKDGQES KKRSSLFRKI 1100
    TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT HSYRSTPDSA 1150
    YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS 1200
    AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP 1250
    RPKSAEPPRS PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF 1300
    HGELALHSLA ESDGETPPIE GPGATRQVAV RRLGRQESPL SLGADPLLPD 1350
    GVQRPMASSK EDSAGGTEAC TPPRATTPGS RTLERDSGCT RHQSVQTEDG 1400
    PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE PARPGVKTQA 1450
    PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA 1500
    SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE 1550
    AVTPAGLTKT GAPSPASLGP 1570
    Length:1,570
    Mass (Da):170,997
    Last modified:September 11, 2007 - v3
    Checksum:iA8F9C868CF241486
    GO

    Sequence cautioni

    The sequence BAC65693.3 differs from that shown. Reason: The sequence differs from that shown because it seems to be derived from a pre-mRNA.
    The sequence AAD50548.1 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti158 – 1581R → P in AAD50548. (PubMed:10404183)Curated
    Sequence conflicti197 – 1982LR → P in AAD50548. (PubMed:10404183)Curated
    Sequence conflicti647 – 6471S → T in AAD50548. (PubMed:10404183)Curated
    Sequence conflicti672 – 6721D → E in AAD50548. (PubMed:10404183)Curated
    Sequence conflicti1190 – 11901H → L in AAD50548. (PubMed:10404183)Curated
    Sequence conflicti1219 – 125032SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548. (PubMed:10404183)CuratedAdd
    BLAST
    Sequence conflicti1454 – 14541L → V in AAD50548. (PubMed:10404183)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077818 mRNA. Translation: AAD50548.1. Frameshift.
    BC054524 mRNA. Translation: AAH54524.1.
    AK122411 Transcribed RNA. Translation: BAC65693.3. Sequence problems.
    CCDSiCCDS40415.1.
    RefSeqiNP_064329.2. NM_019945.2.
    UniGeneiMm.154614.

    Genome annotation databases

    EnsembliENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693.
    GeneIDi56527.
    KEGGimmu:56527.
    UCSCiuc009mof.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077818 mRNA. Translation: AAD50548.1 . Frameshift.
    BC054524 mRNA. Translation: AAH54524.1 .
    AK122411 Transcribed RNA. Translation: BAC65693.3 . Sequence problems.
    CCDSi CCDS40415.1.
    RefSeqi NP_064329.2. NM_019945.2.
    UniGenei Mm.154614.

    3D structure databases

    ProteinModelPortali Q9R1L5.
    SMRi Q9R1L5. Positions 187-691, 967-1059.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208038. 1 interaction.
    IntActi Q9R1L5. 3 interactions.
    MINTi MINT-204974.
    STRINGi 10090.ENSMUSP00000105363.

    PTM databases

    PhosphoSitei Q9R1L5.

    Proteomic databases

    PaxDbi Q9R1L5.
    PRIDEi Q9R1L5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000109741 ; ENSMUSP00000105363 ; ENSMUSG00000053693 .
    GeneIDi 56527.
    KEGGi mmu:56527.
    UCSCi uc009mof.1. mouse.

    Organism-specific databases

    CTDi 22983.
    MGIi MGI:1861901. Mast1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063286.
    HOGENOMi HOG000046662.
    HOVERGENi HBG052414.
    InParanoidi Q9R1L5.
    KOi K08789.
    OMAi GCTRHQS.
    OrthoDBi EOG7PK8ZC.
    PhylomeDBi Q9R1L5.
    TreeFami TF313149.

    Miscellaneous databases

    NextBioi 312870.
    PROi Q9R1L5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9R1L5.
    CleanExi MM_MAST1.
    Genevestigatori Q9R1L5.

    Family and domain databases

    Gene3Di 1.20.1480.20. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR011009. Kinase-like_dom.
    IPR015022. MA_Ser/Thr_Kinase_dom.
    IPR028777. MAST1.
    IPR023142. MAST_pre-PK_dom.
    IPR001478. PDZ.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24356:SF150. PTHR24356:SF150. 1 hit.
    Pfami PF08926. DUF1908. 1 hit.
    PF00595. PDZ. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF140482. SSF140482. 1 hit.
    SSF50156. SSF50156. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50106. PDZ. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases."
      Lumeng C., Phelps S., Crawford G.E., Walden P.D., Barald K., Chamberlain J.S.
      Nat. Neurosci. 2:611-617(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SNTB2.
      Strain: C57BL/10Imported.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6Imported.
      Tissue: BrainImported.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 337-1570.
      Tissue: BrainImported.
    4. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
      Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiMAST1_MOUSE
    AccessioniPrimary (citable) accession number: Q9R1L5
    Secondary accession number(s): Q7TQG9, Q80TN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3