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Q9R1L5

- MAST1_MOUSE

UniProt

Q9R1L5 - MAST1_MOUSE

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Protein
Microtubule-associated serine/threonine-protein kinase 1
Gene
Mast1, Kiaa0973, Sast
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei404 – 4041ATP By similarityBy similarity
Active sitei498 – 4981Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi381 – 3899ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cytoskeleton organization Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated serine/threonine-protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
Syntrophin-associated serine/threonine-protein kinase
Gene namesi
Name:Mast1
Synonyms:Kiaa0973, Sast
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1861901. Mast1.

Subcellular locationi

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton
Note: Colocalizes with syntrophins at the cell membrane.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15701570Microtubule-associated serine/threonine-protein kinase 1
PRO_0000086310Add
BLAST

Proteomic databases

PaxDbiQ9R1L5.
PRIDEiQ9R1L5.

PTM databases

PhosphoSiteiQ9R1L5.

Expressioni

Tissue specificityi

Expressed in the vascular endothelium and in proximity to neuronal nuclei throughout the cortex and cerebellum. Also detected in ependymal cells, the choroid plexus, in developing spermatid acrosomes and in both the cell bodies and axonal processes of motor neurons. Observed as punctate clusters in the processes of interneurons and along the cell body periphery.1 Publication

Gene expression databases

BgeeiQ9R1L5.
CleanExiMM_MAST1.
GenevestigatoriQ9R1L5.

Interactioni

Subunit structurei

Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density. Interacts with SNTB2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTENP604843EBI-491771,EBI-696162From a different organism.

Protein-protein interaction databases

BioGridi208038. 1 interaction.
IntActiQ9R1L5. 3 interactions.
MINTiMINT-204974.
STRINGi10090.ENSMUSP00000105363.

Structurei

3D structure databases

ProteinModelPortaliQ9R1L5.
SMRiQ9R1L5. Positions 187-691, 967-1059.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini375 – 648274Protein kinase
Add
BLAST
Domaini649 – 72072AGC-kinase C-terminal
Add
BLAST
Domaini968 – 105689PDZ
Add
BLAST

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000046662.
HOVERGENiHBG052414.
InParanoidiQ9R1L5.
KOiK08789.
OMAiGCTRHQS.
OrthoDBiEOG7PK8ZC.
PhylomeDBiQ9R1L5.
TreeFamiTF313149.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028777. MAST1.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24356:SF150. PTHR24356:SF150. 1 hit.
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1L5-1 [UniParc]FASTAAdd to Basket

« Hide

MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH     50
SPLPGHLGSS PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG 100
TNTPSSTVSS SCSSQERLHQ LPYQPTVDEL HFLSKHFGST ESITDEDGGR 150
RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH VYKERFPKAT AQMEEKLRDF 200
ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI TTVYFYELQE 250
NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY 300
HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN 350
TPEQDDTSEG RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ 400
RFAMKKINKQ NLILRNQIQQ AFVERDILTF AENPFVVGMF CSFETRRHLC 450
MVMEYVEGGD CATLLKNIGA LPVEMARMYF AETVLALEYL HNYGIVHRDL 500
KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD AREFLDKQVC 550
GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ 600
VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR 650
DLDWTGLLRQ KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP 700
VEIRQFSSCS PRFSKVYSSM EQLSQHEPKT PVSASGASKR DPSAKGPEEK 750
VAGKREGLGG LTLREKTWRG GSPEIKRFSA SEASFLEGEA SPPLGARRRF 800
SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP KEAVQGEGTS 850
TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV 900
EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS 950
RDSSPSRDYS PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH 1000
IVWHVEEGGP AQEAGLCAGD LITHVNGEPV HGMVHPEVVE LILKSGNKVA 1050
VTTTPFENTS IRIGPARRSS YKAKMARRNK RPSAKDGQES KKRSSLFRKI 1100
TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT HSYRSTPDSA 1150
YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS 1200
AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP 1250
RPKSAEPPRS PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF 1300
HGELALHSLA ESDGETPPIE GPGATRQVAV RRLGRQESPL SLGADPLLPD 1350
GVQRPMASSK EDSAGGTEAC TPPRATTPGS RTLERDSGCT RHQSVQTEDG 1400
PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE PARPGVKTQA 1450
PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA 1500
SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE 1550
AVTPAGLTKT GAPSPASLGP 1570
Length:1,570
Mass (Da):170,997
Last modified:September 11, 2007 - v3
Checksum:iA8F9C868CF241486
GO

Sequence cautioni

The sequence BAC65693.3 differs from that shown. Reason: The sequence differs from that shown because it seems to be derived from a pre-mRNA.
The sequence AAD50548.1 differs from that shown. Reason: Frameshift at several positions.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581R → P in AAD50548. 1 Publication
Sequence conflicti197 – 1982LR → P in AAD50548. 1 Publication
Sequence conflicti647 – 6471S → T in AAD50548. 1 Publication
Sequence conflicti672 – 6721D → E in AAD50548. 1 Publication
Sequence conflicti1190 – 11901H → L in AAD50548. 1 Publication
Sequence conflicti1219 – 125032SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548. 1 Publication
Add
BLAST
Sequence conflicti1454 – 14541L → V in AAD50548. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077818 mRNA. Translation: AAD50548.1. Frameshift.
BC054524 mRNA. Translation: AAH54524.1.
AK122411 Transcribed RNA. Translation: BAC65693.3. Sequence problems.
CCDSiCCDS40415.1.
RefSeqiNP_064329.2. NM_019945.2.
UniGeneiMm.154614.

Genome annotation databases

EnsembliENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693.
GeneIDi56527.
KEGGimmu:56527.
UCSCiuc009mof.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077818 mRNA. Translation: AAD50548.1 . Frameshift.
BC054524 mRNA. Translation: AAH54524.1 .
AK122411 Transcribed RNA. Translation: BAC65693.3 . Sequence problems.
CCDSi CCDS40415.1.
RefSeqi NP_064329.2. NM_019945.2.
UniGenei Mm.154614.

3D structure databases

ProteinModelPortali Q9R1L5.
SMRi Q9R1L5. Positions 187-691, 967-1059.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208038. 1 interaction.
IntActi Q9R1L5. 3 interactions.
MINTi MINT-204974.
STRINGi 10090.ENSMUSP00000105363.

PTM databases

PhosphoSitei Q9R1L5.

Proteomic databases

PaxDbi Q9R1L5.
PRIDEi Q9R1L5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000109741 ; ENSMUSP00000105363 ; ENSMUSG00000053693 .
GeneIDi 56527.
KEGGi mmu:56527.
UCSCi uc009mof.1. mouse.

Organism-specific databases

CTDi 22983.
MGIi MGI:1861901. Mast1.
Rougei Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063286.
HOGENOMi HOG000046662.
HOVERGENi HBG052414.
InParanoidi Q9R1L5.
KOi K08789.
OMAi GCTRHQS.
OrthoDBi EOG7PK8ZC.
PhylomeDBi Q9R1L5.
TreeFami TF313149.

Miscellaneous databases

NextBioi 312870.
PROi Q9R1L5.
SOURCEi Search...

Gene expression databases

Bgeei Q9R1L5.
CleanExi MM_MAST1.
Genevestigatori Q9R1L5.

Family and domain databases

Gene3Di 1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR028777. MAST1.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24356:SF150. PTHR24356:SF150. 1 hit.
Pfami PF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00228. PDZ. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Interactions between beta 2-syntrophin and a family of microtubule-associated serine/threonine kinases."
    Lumeng C., Phelps S., Crawford G.E., Walden P.D., Barald K., Chamberlain J.S.
    Nat. Neurosci. 2:611-617(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SNTB2.
    Strain: C57BL/10.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 337-1570.
    Tissue: Brain.
  4. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiMAST1_MOUSE
AccessioniPrimary (citable) accession number: Q9R1L5
Secondary accession number(s): Q7TQG9, Q80TN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: September 11, 2007
Last modified: July 9, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi