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Q9R1J8 (P3H1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Prolyl 3-hydroxylase 1
EC=1.14.11.7
Alternative name(s):
Leucine- and proline-enriched proteoglycan 1
Short name=Leprecan-1
Gene names
Name:Lepre1
Synonyms:P3h1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length728 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Iron By similarity.

Ascorbate By similarity.

Subcellular location

Endoplasmic reticulum. Secreted › extracellular space › extracellular matrix. Note: Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan (CSPG). Ref.1

Tissue specificity

Expressed in basement membranes of cardiac muscle, skeletal muscle, central nervous system, intestinal tract, trachea, ear, skin, liver and kidney. In kidney, localizes to the glomerular basement membrane, mesangial matrix and Bowman's capsule of the nephron. In the renal parenchyma, expressed in the basement membranes of tubules and blood vessels. In the ear and trachea, localizes to the perimeter of resident chondrocytes in lacunae. Ref.1

Post-translational modification

O-glycosylated; chondroitin sulfate. Ref.1

Sequence similarities

Belongs to the leprecan family.

Contains 1 Fe2OG dioxygenase domain.

Contains 4 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 Potential
Chain15 – 728714Prolyl 3-hydroxylase 1
PRO_0000240354

Regions

Repeat25 – 5834TPR 1
Repeat135 – 16834TPR 2
Repeat197 – 23034TPR 3
Repeat293 – 32634TPR 4
Domain556 – 670115Fe2OG dioxygenase
Coiled coil393 – 43139 Potential
Motif725 – 7284Prevents secretion from ER Potential

Sites

Active site6611 By similarity
Metal binding5791Iron
Metal binding5811Iron
Metal binding6511Iron

Amino acid modifications

Glycosylation3081N-linked (GlcNAc...) Potential
Glycosylation4501N-linked (GlcNAc...) Potential
Glycosylation4591N-linked (GlcNAc...) Potential
Glycosylation5321N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9R1J8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 06AFE6972BF3EE1F

FASTA72882,390
        10         20         30         40         50         60 
MVAVAAAAAS RATAESEPEW NVAAPDLLYA EGTAAYARGD WPGVVLNMER ALRSRAALRA 

        70         80         90        100        110        120 
LRLRCRTRCA TELPWAPDLD LGPASSLNHD PGAAALHDLR FFGALLRRAA CLRRCLGPPS 

       130        140        150        160        170        180 
AHLLSEELDL EFNKRSPYNY LQVAYFKINK LEKAVAAAHT FFVGNPEHME MRQNLDYYQT 

       190        200        210        220        230        240 
MSGVKEEDFK DLEAKPHMHE FRLGVRLYSE EKPLEAVPHL EAALQEYFVA DEECRALCEG 

       250        260        270        280        290        300 
PYDYDGYNYL DYSADLFQAI TDHYVQVLSC KQNCVTELAS HPSREKPFED FLPSHYNYLQ 

       310        320        330        340        350        360 
FAYYNIGNYT QAIECAKTYL LFFPNDEVMS QNLAYYTAVL GEEEASSISP RENAQEYRHR 

       370        380        390        400        410        420 
SLLEKELLFF AYDIFGIPFV DPDSWTPEEV IPKRLQEKQK SERETAVRIS QEIGNLMKEI 

       430        440        450        460        470        480 
ETLVEEKTKE SLDVSRLTRE GGPLLYEGIN LTMNSKVLNG SQRVVMDGVI SDDECQELQR 

       490        500        510        520        530        540 
LTNAAATSGD GYRGQTSPHT PNEKFYGVTV LKALKLGQEG KVPLQSAHMY YNVTEKVRRV 

       550        560        570        580        590        600 
MESYFRLDTP LYFSYSHLVC RTAIEESQAE RKDSSHPVHV DNCILNAESL VCIKEPPAYT 

       610        620        630        640        650        660 
FRDYSAILYL NGDFDGGNFY FTELDAKTVT AEVQPQCGRA VGFSSGTENP HGVKAVTRGQ 

       670        680        690        700        710        720 
RCAIALWFTL DPRHSERDRV QADDLVKMLF SPEEVDLPQE QPLPDQQGSP KPGEESLSDR 


ESQPKDEL

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References

[1]"Molecular characterization of a novel basement membrane-associated proteoglycan, leprecan."
Wassenhove-McCarthy D.J., McCarthy K.J.
J. Biol. Chem. 274:25004-25017(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF087433 mRNA. Translation: AAD51875.1.
IPIIPI00207325.
RefSeqNP_446119.1. NM_053667.1.
UniGeneRn.13741.

3D structure databases

ProteinModelPortalQ9R1J8.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9R1J8. 1 interaction.
MINTMINT-1775508.

Proteomic databases

PaxDbQ9R1J8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID114200.
KEGGrno:114200.
UCSCRGD:628823. rat.

Organism-specific databases

CTD64175.
RGD628823. Lepre1.

Phylogenomic databases

eggNOGNOG269251.
HOGENOMHOG000231087.
HOVERGENHBG053224.
KOK08134.

Gene expression databases

ArrayExpressQ9R1J8.
GenevestigatorQ9R1J8.
GermOnlineENSRNOG00000007538. Rattus norvegicus.

Family and domain databases

Gene3D1.25.40.10. 3 hits.
InterProIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. False negative.
PS50293. TPR_REGION. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio618365.

Entry information

Entry nameP3H1_RAT
AccessionPrimary (citable) accession number: Q9R1J8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: May 1, 2000
Last modified: April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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