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Protein

Prolyl 3-hydroxylase 1

Gene

P3h1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts (By similarity).By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi579Iron1
Metal bindingi581Iron1
Metal bindingi651Iron1
Active sitei661By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 1Imported (EC:1.14.11.7)
Alternative name(s):
Leucine- and proline-enriched proteoglycan 11 Publication
Short name:
Leprecan-11 Publication
Gene namesi
Name:P3h1Imported
Synonyms:Lepre11 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628823. P3h1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • endoplasmic reticulum Source: GO_Central

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 14Sequence analysisAdd BLAST14
ChainiPRO_000024035415 – 728Prolyl 3-hydroxylase 1Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi450N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi459N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi532N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

O-glycosylated; chondroitin sulfate.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9R1J8.
PRIDEiQ9R1J8.

Expressioni

Tissue specificityi

Expressed in basement membranes of cardiac muscle, skeletal muscle, central nervous system, intestinal tract, trachea, ear, skin, liver and kidney. In kidney, localizes to the glomerular basement membrane, mesangial matrix and Bowman's capsule of the nephron. In the renal parenchyma, expressed in the basement membranes of tubules and blood vessels. In the ear and trachea, localizes to the perimeter of resident chondrocytes in lacunae.1 Publication

Interactioni

Protein-protein interaction databases

IntActiQ9R1J8. 1 interactor.
MINTiMINT-1775508.
STRINGi10116.ENSRNOP00000010395.

Structurei

3D structure databases

ProteinModelPortaliQ9R1J8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati25 – 58TPR 1Add BLAST34
Repeati135 – 168TPR 2Add BLAST34
Repeati197 – 230TPR 3Add BLAST34
Repeati293 – 326TPR 4Add BLAST34
Domaini556 – 670Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST115

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili393 – 431Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi725 – 728Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the leprecan family.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG4459. Eukaryota.
ENOG410XPXF. LUCA.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ9R1J8.
KOiK08134.
PhylomeDBiQ9R1J8.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiView protein in InterPro
IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
PfamiView protein in Pfam
PF13640. 2OG-FeII_Oxy_3. 1 hit.
SMARTiView protein in SMART
SM00702. P4Hc. 1 hit.
PROSITEiView protein in PROSITE
PS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R1J8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAVAAAAAS RATAESEPEW NVAAPDLLYA EGTAAYARGD WPGVVLNMER
60 70 80 90 100
ALRSRAALRA LRLRCRTRCA TELPWAPDLD LGPASSLNHD PGAAALHDLR
110 120 130 140 150
FFGALLRRAA CLRRCLGPPS AHLLSEELDL EFNKRSPYNY LQVAYFKINK
160 170 180 190 200
LEKAVAAAHT FFVGNPEHME MRQNLDYYQT MSGVKEEDFK DLEAKPHMHE
210 220 230 240 250
FRLGVRLYSE EKPLEAVPHL EAALQEYFVA DEECRALCEG PYDYDGYNYL
260 270 280 290 300
DYSADLFQAI TDHYVQVLSC KQNCVTELAS HPSREKPFED FLPSHYNYLQ
310 320 330 340 350
FAYYNIGNYT QAIECAKTYL LFFPNDEVMS QNLAYYTAVL GEEEASSISP
360 370 380 390 400
RENAQEYRHR SLLEKELLFF AYDIFGIPFV DPDSWTPEEV IPKRLQEKQK
410 420 430 440 450
SERETAVRIS QEIGNLMKEI ETLVEEKTKE SLDVSRLTRE GGPLLYEGIN
460 470 480 490 500
LTMNSKVLNG SQRVVMDGVI SDDECQELQR LTNAAATSGD GYRGQTSPHT
510 520 530 540 550
PNEKFYGVTV LKALKLGQEG KVPLQSAHMY YNVTEKVRRV MESYFRLDTP
560 570 580 590 600
LYFSYSHLVC RTAIEESQAE RKDSSHPVHV DNCILNAESL VCIKEPPAYT
610 620 630 640 650
FRDYSAILYL NGDFDGGNFY FTELDAKTVT AEVQPQCGRA VGFSSGTENP
660 670 680 690 700
HGVKAVTRGQ RCAIALWFTL DPRHSERDRV QADDLVKMLF SPEEVDLPQE
710 720
QPLPDQQGSP KPGEESLSDR ESQPKDEL
Length:728
Mass (Da):82,390
Last modified:May 1, 2000 - v1
Checksum:i06AFE6972BF3EE1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087433 mRNA. Translation: AAD51875.1.
RefSeqiNP_446119.1. NM_053667.1.
UniGeneiRn.13741.

Genome annotation databases

GeneIDi114200.
KEGGirno:114200.
UCSCiRGD:628823. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiP3H1_RAT
AccessioniPrimary (citable) accession number: Q9R1J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: May 1, 2000
Last modified: May 10, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families