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Protein

Myocilin

Gene

Myoc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling. Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling. Mediates myelination in the peripheral nervous system through ERBB2/ERBB3 signaling. Plays a role as a regulator of muscle hypertrophy through the components of dystrophin-associated protein complex. Involved in positive regulation of mitochondrial depolarization. Plays a role in neurite outgrowth. May participate in the obstruction of fluid outflow in the trabecular meshwork.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi378CalciumBy similarity1
Metal bindingi426CalciumBy similarity1
Metal bindingi427Calcium; via carbonyl oxygenBy similarity1
Metal bindingi475Calcium; via carbonyl oxygenBy similarity1
Metal bindingi476CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myocilin
Alternative name(s):
Trabecular meshwork-induced glucocorticoid response protein
Cleaved into the following 2 chains:
Alternative name(s):
Myocilin 20 kDa N-terminal fragment
Alternative name(s):
Myocilin 35 kDa N-terminal fragment
Gene namesi
Name:Myoc
Synonyms:Tigr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620430. Myoc.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasmic vesicle, Endoplasmic reticulum, Extracellular matrix, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31By similarityAdd BLAST31
ChainiPRO_000002008832 – 502MyocilinAdd BLAST471
ChainiPRO_000042874732 – 225Myocilin, N-terminal fragmentBy similarityAdd BLAST194
ChainiPRO_0000428748226 – 502Myocilin, C-terminal fragmentBy similarityAdd BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi229N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi243 ↔ 431PROSITE-ProRule annotationBy similarity

Post-translational modificationi

Palmitoylated.By similarity
Undergoes a calcium-dependent proteolytic cleavage at Gln-225 by CAPN2 in the endoplasmic reticulum. The result is the production of two fragments, one of 35 kDa containing the C-terminal olfactomedin-like domain, and another of 20 kDa containing the N-terminal leucine zipper-like domain (By similarity).By similarity
Glycosylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei225 – 226Cleavage; by CAPN2By similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ9R1J4.

PTM databases

iPTMnetiQ9R1J4.
PhosphoSitePlusiQ9R1J4.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle and retina. Also detected at lower levels in thyroid gland but not in other endocrine glands such as the adrenal or pituitary glands.1 Publication

Inductioni

Up-regulated by dexamethasone.1 Publication

Interactioni

Subunit structurei

Homodimer (via N-terminus). Can also form higher oligomers. Interacts with OLFM3, FN1, NRCAM, GLDN and NFASC. Interacts (via N-terminus) with MYL2. Interacts with SFRP1, FRZB, FZD7, FZD10, FZD1 and WIF1; regulates Wnt signaling (By similarity). Interacts with SNTA1; regulates muscle hypertrophy. Interacts with ERBB2 and ERBB3; acivates ERBB2-ERBB3 signaling pathway. Interacts with SNCG; affects its secretion and its aggregation (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9R1J4. 1 interactor.
STRINGi10116.ENSRNOP00000004386.

Structurei

3D structure databases

ProteinModelPortaliQ9R1J4.
SMRiQ9R1J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini242 – 501Olfactomedin-likePROSITE-ProRule annotationAdd BLAST260

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili82 – 183Sequence analysisAdd BLAST102

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiENOG410INPA. Eukaryota.
ENOG410YBJJ. LUCA.
HOGENOMiHOG000059654.
HOVERGENiHBG105662.
InParanoidiQ9R1J4.
PhylomeDBiQ9R1J4.

Family and domain databases

InterProiView protein in InterPro
IPR031213. Myocilin.
IPR003112. Olfac-like_dom.
PANTHERiPTHR23192:SF48. PTHR23192:SF48. 1 hit.
PfamiView protein in Pfam
PF02191. OLF. 1 hit.
SMARTiView protein in SMART
SM00284. OLF. 1 hit.
PROSITEiView protein in PROSITE
PS51132. OLF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R1J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSCAYCCSC GPKMPALQLL FLACLVWGMG ARTAQFRKAN DRSGRCQYTF
60 70 80 90 100
TVASPSESSC PREDQAMSAI QDLQRDSSIQ HADLESTKAR VRSLESLLHQ
110 120 130 140 150
MTSGGVTGTQ EVQEGLQGQL GALRRERDQL ETQTRDLEVA YNNLLRDKSA
160 170 180 190 200
LEEEKRQLEQ ENKDLARRLE GSSQEVARLR RGQCPSTHHP SQDMLPGSRE
210 220 230 240 250
VSQWNLDTLA FQELKSELTE VPASQILKNQ SGHPRSKEGD KGCGVLMWVG
260 270 280 290 300
EPVTLRTAET ITGKYGVWMR DPKPTHPYTQ ETTWRIDTVG TGIRQVFEYS
310 320 330 340 350
QISQFEQGYP SKVHVLPQAL ESTGAVVYSG SLYFQGAESR TVLRYELNTE
360 370 380 390 400
TVKAEKEIPG AGYHGQFPYA WGGYTDIDLA VDESGLWVIY STEETRGAIV
410 420 430 440 450
LSKLNPENLE LESTWETNIR KQSVANAFVI CGILYTVSSY SSVHATINFA
460 470 480 490 500
YDTNTGISKT LTIPFKNRYK YSSMVDYNPL ERKLFAWDNF NMVTYDIKLS

EM
Length:502
Mass (Da):56,442
Last modified:May 1, 2000 - v1
Checksum:i2FE8FBE53CF48BBA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6Y → R in BAA34199 (PubMed:10833334).Curated1
Sequence conflicti329S → A in BAA34199 (PubMed:10833334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093567 mRNA. Translation: AAD46401.1.
AB019393 mRNA. Translation: BAA34199.1.
RefSeqiNP_110492.1. NM_030865.1.
UniGeneiRn.30051.

Genome annotation databases

GeneIDi81523.
KEGGirno:81523.
UCSCiRGD:620430. rat.

Similar proteinsi

Entry informationi

Entry nameiMYOC_RAT
AccessioniPrimary (citable) accession number: Q9R1J4
Secondary accession number(s): Q9Z2Y4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome