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Q9R1J0

- NSDHL_MOUSE

UniProt

Q9R1J0 - NSDHL_MOUSE

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Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene
Nsdhl
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis By similarity.

Catalytic activityi

A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611Proton acceptor By similarity
Binding sitei165 – 1651NAD By similarity

GO - Molecular functioni

  1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: InterPro
  2. sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity Source: UniProtKB-EC

GO - Biological processi

  1. cholesterol biosynthetic process Source: UniProtKB-KW
  2. cholesterol metabolic process Source: MGI
  3. hair follicle development Source: MGI
  4. labyrinthine layer blood vessel development Source: MGI
  5. smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_208531. Cholesterol biosynthesis.
UniPathwayiUPA00770; UER00757.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
Gene namesi
Name:Nsdhl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1099438. Nsdhl.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Lipid droplet By similarity
Note: Trafficking through the Golgi is necessary for ER membrane localization By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei287 – 30721Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. lipid particle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingPRO_0000087800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9R1J0.
PaxDbiQ9R1J0.
PRIDEiQ9R1J0.

PTM databases

PhosphoSiteiQ9R1J0.

Expressioni

Gene expression databases

ArrayExpressiQ9R1J0.
BgeeiQ9R1J0.
CleanExiMM_NSDHL.
GenevestigatoriQ9R1J0.

Interactioni

Protein-protein interaction databases

IntActiQ9R1J0. 3 interactions.
MINTiMINT-1837653.
STRINGi10090.ENSMUSP00000033715.

Structurei

3D structure databases

ProteinModelPortaliQ9R1J0.
SMRiQ9R1J0. Positions 26-355.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi359 – 3624Prevents secretion from ER By similarity

Sequence similaritiesi

Belongs to the 3-beta-HSD family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG054675.
InParanoidiQ9R1J0.
KOiK07748.
OMAiEPMRDQV.
OrthoDBiEOG77M8NP.
PhylomeDBiQ9R1J0.
TreeFamiTF354279.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1J0-1 [UniParc]FASTAAdd to Basket

« Hide

MEQAVHGESK RGQVTGTHLT NDISKAKKCT VIGGSGFLGQ HMVEQLLERG    50
YTVNVFDIHQ GFDNPRVQFF IGDLCNQQDL YPALKGVSTV FHCASPPPYS 100
NNKELFYRVN FIGTKTVIET CREAGVQKLI LTSSASVVFE GVDIKNGTED 150
LPYAMKPIDY YTETKILQER AVLDANDPKK NFLTAAIRPH GIFGPRDPQL 200
VPILIDAARK GKMKFMIGNG ENLVDFTFVE NVVHGHILAA EHLSQDAALG 250
GKAFHITNDE PIPFWTFLSR ILTGLNYEAP KYHIPYWMAY YLAFLLSLLV 300
MVVSPLIQIQ PTFTPIRVAL AGTFHYYSCE KAKKLFGYRP LVTMDEAVER 350
TVQSFHHLRK DK 362
Length:362
Mass (Da):40,686
Last modified:May 1, 2000 - v1
Checksum:i4DB53EC0314885C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF100198 mRNA. Translation: AAD38448.1.
AL021127 Genomic DNA. Translation: CAA15948.2.
CCDSiCCDS30190.1.
RefSeqiNP_035071.3. NM_010941.3.
UniGeneiMm.38792.

Genome annotation databases

EnsembliENSMUST00000033715; ENSMUSP00000033715; ENSMUSG00000031349.
GeneIDi18194.
KEGGimmu:18194.
UCSCiuc009tkv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF100198 mRNA. Translation: AAD38448.1 .
AL021127 Genomic DNA. Translation: CAA15948.2 .
CCDSi CCDS30190.1.
RefSeqi NP_035071.3. NM_010941.3.
UniGenei Mm.38792.

3D structure databases

ProteinModelPortali Q9R1J0.
SMRi Q9R1J0. Positions 26-355.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9R1J0. 3 interactions.
MINTi MINT-1837653.
STRINGi 10090.ENSMUSP00000033715.

PTM databases

PhosphoSitei Q9R1J0.

Proteomic databases

MaxQBi Q9R1J0.
PaxDbi Q9R1J0.
PRIDEi Q9R1J0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033715 ; ENSMUSP00000033715 ; ENSMUSG00000031349 .
GeneIDi 18194.
KEGGi mmu:18194.
UCSCi uc009tkv.1. mouse.

Organism-specific databases

CTDi 50814.
MGIi MGI:1099438. Nsdhl.

Phylogenomic databases

eggNOGi COG0451.
GeneTreei ENSGT00550000074557.
HOGENOMi HOG000167989.
HOVERGENi HBG054675.
InParanoidi Q9R1J0.
KOi K07748.
OMAi EPMRDQV.
OrthoDBi EOG77M8NP.
PhylomeDBi Q9R1J0.
TreeFami TF354279.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00757 .
Reactomei REACT_208531. Cholesterol biosynthesis.

Miscellaneous databases

ChiTaRSi NSDHL. mouse.
NextBioi 293544.
PROi Q9R1J0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9R1J0.
Bgeei Q9R1J0.
CleanExi MM_NSDHL.
Genevestigatori Q9R1J0.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01073. 3Beta_HSD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6.

Entry informationi

Entry nameiNSDHL_MOUSE
AccessioniPrimary (citable) accession number: Q9R1J0
Secondary accession number(s): O55109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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