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Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene

Nsdhl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.By similarity

Catalytic activityi

A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

Pathway:izymosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611Proton acceptorBy similarity
Binding sitei165 – 1651NADBy similarity

GO - Molecular functioni

GO - Biological processi

  • cholesterol biosynthetic process Source: UniProtKB-KW
  • cholesterol metabolic process Source: MGI
  • hair follicle development Source: MGI
  • labyrinthine layer blood vessel development Source: MGI
  • smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_345006. Cholesterol biosynthesis.
UniPathwayiUPA00770; UER00757.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
Gene namesi
Name:Nsdhl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1099438. Nsdhl.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei287 – 30721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingPRO_0000087800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9R1J0.
PaxDbiQ9R1J0.
PRIDEiQ9R1J0.

PTM databases

PhosphoSiteiQ9R1J0.

Expressioni

Gene expression databases

BgeeiQ9R1J0.
CleanExiMM_NSDHL.
ExpressionAtlasiQ9R1J0. baseline and differential.
GenevisibleiQ9R1J0. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9R1J0. 3 interactions.
MINTiMINT-1837653.
STRINGi10090.ENSMUSP00000033715.

Structurei

3D structure databases

ProteinModelPortaliQ9R1J0.
SMRiQ9R1J0. Positions 26-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi359 – 3624Prevents secretion from ERBy similarity

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG054675.
InParanoidiQ9R1J0.
KOiK07748.
OMAiEPMRDQV.
OrthoDBiEOG77M8NP.
PhylomeDBiQ9R1J0.
TreeFamiTF354279.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQAVHGESK RGQVTGTHLT NDISKAKKCT VIGGSGFLGQ HMVEQLLERG
60 70 80 90 100
YTVNVFDIHQ GFDNPRVQFF IGDLCNQQDL YPALKGVSTV FHCASPPPYS
110 120 130 140 150
NNKELFYRVN FIGTKTVIET CREAGVQKLI LTSSASVVFE GVDIKNGTED
160 170 180 190 200
LPYAMKPIDY YTETKILQER AVLDANDPKK NFLTAAIRPH GIFGPRDPQL
210 220 230 240 250
VPILIDAARK GKMKFMIGNG ENLVDFTFVE NVVHGHILAA EHLSQDAALG
260 270 280 290 300
GKAFHITNDE PIPFWTFLSR ILTGLNYEAP KYHIPYWMAY YLAFLLSLLV
310 320 330 340 350
MVVSPLIQIQ PTFTPIRVAL AGTFHYYSCE KAKKLFGYRP LVTMDEAVER
360
TVQSFHHLRK DK
Length:362
Mass (Da):40,686
Last modified:May 1, 2000 - v1
Checksum:i4DB53EC0314885C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100198 mRNA. Translation: AAD38448.1.
AL021127 Genomic DNA. Translation: CAA15948.2.
CCDSiCCDS30190.1.
RefSeqiNP_035071.3. NM_010941.3.
UniGeneiMm.38792.

Genome annotation databases

EnsembliENSMUST00000033715; ENSMUSP00000033715; ENSMUSG00000031349.
GeneIDi18194.
KEGGimmu:18194.
UCSCiuc009tkv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100198 mRNA. Translation: AAD38448.1.
AL021127 Genomic DNA. Translation: CAA15948.2.
CCDSiCCDS30190.1.
RefSeqiNP_035071.3. NM_010941.3.
UniGeneiMm.38792.

3D structure databases

ProteinModelPortaliQ9R1J0.
SMRiQ9R1J0. Positions 26-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R1J0. 3 interactions.
MINTiMINT-1837653.
STRINGi10090.ENSMUSP00000033715.

PTM databases

PhosphoSiteiQ9R1J0.

Proteomic databases

MaxQBiQ9R1J0.
PaxDbiQ9R1J0.
PRIDEiQ9R1J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033715; ENSMUSP00000033715; ENSMUSG00000031349.
GeneIDi18194.
KEGGimmu:18194.
UCSCiuc009tkv.1. mouse.

Organism-specific databases

CTDi50814.
MGIiMGI:1099438. Nsdhl.

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG054675.
InParanoidiQ9R1J0.
KOiK07748.
OMAiEPMRDQV.
OrthoDBiEOG77M8NP.
PhylomeDBiQ9R1J0.
TreeFamiTF354279.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00757.
ReactomeiREACT_345006. Cholesterol biosynthesis.

Miscellaneous databases

ChiTaRSiNsdhl. mouse.
NextBioi293544.
PROiQ9R1J0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R1J0.
CleanExiMM_NSDHL.
ExpressionAtlasiQ9R1J0. baseline and differential.
GenevisibleiQ9R1J0. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6.

Entry informationi

Entry nameiNSDHL_MOUSE
AccessioniPrimary (citable) accession number: Q9R1J0
Secondary accession number(s): O55109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 2000
Last modified: June 24, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.