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Q9R1J0

- NSDHL_MOUSE

UniProt

Q9R1J0 - NSDHL_MOUSE

Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene

Nsdhl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.By similarity

    Catalytic activityi

    A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei161 – 1611Proton acceptorBy similarity
    Binding sitei165 – 1651NADBy similarity

    GO - Molecular functioni

    1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: InterPro
    2. sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity Source: UniProtKB-EC

    GO - Biological processi

    1. cholesterol biosynthetic process Source: UniProtKB-KW
    2. cholesterol metabolic process Source: MGI
    3. hair follicle development Source: MGI
    4. labyrinthine layer blood vessel development Source: MGI
    5. smoothened signaling pathway Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_208531. Cholesterol biosynthesis.
    UniPathwayiUPA00770; UER00757.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
    Gene namesi
    Name:Nsdhl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1099438. Nsdhl.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity. Lipid droplet By similarity
    Note: Trafficking through the Golgi is necessary for ER membrane localization.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. lipid particle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Lipid droplet, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 362362Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingPRO_0000087800Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9R1J0.
    PaxDbiQ9R1J0.
    PRIDEiQ9R1J0.

    PTM databases

    PhosphoSiteiQ9R1J0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9R1J0.
    BgeeiQ9R1J0.
    CleanExiMM_NSDHL.
    GenevestigatoriQ9R1J0.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9R1J0. 3 interactions.
    MINTiMINT-1837653.
    STRINGi10090.ENSMUSP00000033715.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R1J0.
    SMRiQ9R1J0. Positions 26-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei287 – 30721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi359 – 3624Prevents secretion from ERBy similarity

    Sequence similaritiesi

    Belongs to the 3-beta-HSD family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0451.
    GeneTreeiENSGT00550000074557.
    HOGENOMiHOG000167989.
    HOVERGENiHBG054675.
    InParanoidiQ9R1J0.
    KOiK07748.
    OMAiEPMRDQV.
    OrthoDBiEOG77M8NP.
    PhylomeDBiQ9R1J0.
    TreeFamiTF354279.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01073. 3Beta_HSD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R1J0-1 [UniParc]FASTAAdd to Basket

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    MEQAVHGESK RGQVTGTHLT NDISKAKKCT VIGGSGFLGQ HMVEQLLERG    50
    YTVNVFDIHQ GFDNPRVQFF IGDLCNQQDL YPALKGVSTV FHCASPPPYS 100
    NNKELFYRVN FIGTKTVIET CREAGVQKLI LTSSASVVFE GVDIKNGTED 150
    LPYAMKPIDY YTETKILQER AVLDANDPKK NFLTAAIRPH GIFGPRDPQL 200
    VPILIDAARK GKMKFMIGNG ENLVDFTFVE NVVHGHILAA EHLSQDAALG 250
    GKAFHITNDE PIPFWTFLSR ILTGLNYEAP KYHIPYWMAY YLAFLLSLLV 300
    MVVSPLIQIQ PTFTPIRVAL AGTFHYYSCE KAKKLFGYRP LVTMDEAVER 350
    TVQSFHHLRK DK 362
    Length:362
    Mass (Da):40,686
    Last modified:May 1, 2000 - v1
    Checksum:i4DB53EC0314885C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF100198 mRNA. Translation: AAD38448.1.
    AL021127 Genomic DNA. Translation: CAA15948.2.
    CCDSiCCDS30190.1.
    RefSeqiNP_035071.3. NM_010941.3.
    UniGeneiMm.38792.

    Genome annotation databases

    EnsembliENSMUST00000033715; ENSMUSP00000033715; ENSMUSG00000031349.
    GeneIDi18194.
    KEGGimmu:18194.
    UCSCiuc009tkv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF100198 mRNA. Translation: AAD38448.1 .
    AL021127 Genomic DNA. Translation: CAA15948.2 .
    CCDSi CCDS30190.1.
    RefSeqi NP_035071.3. NM_010941.3.
    UniGenei Mm.38792.

    3D structure databases

    ProteinModelPortali Q9R1J0.
    SMRi Q9R1J0. Positions 26-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9R1J0. 3 interactions.
    MINTi MINT-1837653.
    STRINGi 10090.ENSMUSP00000033715.

    PTM databases

    PhosphoSitei Q9R1J0.

    Proteomic databases

    MaxQBi Q9R1J0.
    PaxDbi Q9R1J0.
    PRIDEi Q9R1J0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033715 ; ENSMUSP00000033715 ; ENSMUSG00000031349 .
    GeneIDi 18194.
    KEGGi mmu:18194.
    UCSCi uc009tkv.1. mouse.

    Organism-specific databases

    CTDi 50814.
    MGIi MGI:1099438. Nsdhl.

    Phylogenomic databases

    eggNOGi COG0451.
    GeneTreei ENSGT00550000074557.
    HOGENOMi HOG000167989.
    HOVERGENi HBG054675.
    InParanoidi Q9R1J0.
    KOi K07748.
    OMAi EPMRDQV.
    OrthoDBi EOG77M8NP.
    PhylomeDBi Q9R1J0.
    TreeFami TF354279.

    Enzyme and pathway databases

    UniPathwayi UPA00770 ; UER00757 .
    Reactomei REACT_208531. Cholesterol biosynthesis.

    Miscellaneous databases

    ChiTaRSi NSDHL. mouse.
    NextBioi 293544.
    PROi Q9R1J0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R1J0.
    Bgeei Q9R1J0.
    CleanExi MM_NSDHL.
    Genevestigatori Q9R1J0.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01073. 3Beta_HSD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6.

    Entry informationi

    Entry nameiNSDHL_MOUSE
    AccessioniPrimary (citable) accession number: Q9R1J0
    Secondary accession number(s): O55109
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3