Q9R1I1 (CHST4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbohydrate sulfotransferase 4
EC=2.8.2.-

Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
Short name=GST-3
High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
Short name=HEC-GlcNAc6ST
L-selectin ligand sulfotransferase
Short name=LSST
N-acetylglucosamine 6-O-sulfotransferase 2
Short name=GlcNAc6ST-2
Short name=Gn6st-2

Gene names

Name:	Chst4
Synonyms:	Gst3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of SELL ligand sialyl 6-sulfo Lewis X on SELL counter-receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8
Subunit structure	Monomer By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.7.
Tissue specificity	Specifically expressed in HEV. Ref.1
Disruption phenotype	Mice are impaired in lymphocyte homing and exhibit faster lymphocyte rolling and reduced lymphocyte sticking in HEV. The epitope of SELL ligands recognized by the MECA-79 antibody is greatly reduced or abolished in the abluminal aspect of HEV. Ref.5 Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Inflammatory response
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: Compara carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW leukocyte tethering or rolling Inferred from mutant phenotype Ref.6. Source: MGI protein sulfation Inferred from mutant phenotype Ref.6. Source: MGI
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW trans-Golgi network Inferred from electronic annotation. Source: Compara
Molecular_function	N-acetylglucosamine 6-O-sulfotransferase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Carbohydrate sulfotransferase 4		PRO_0000085194
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 388	360	Lumenal Potential
	Nucleotide binding	50 – 56	7	PAPS By similarity
	Nucleotide binding	204 – 212	9	PAPS By similarity
Amino acid modifications
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	369	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	221	1	V → M Ref.2
	Sequence conflict	221	1	V → M Ref.3
	Sequence conflict	306	1	H → Y Ref.2
	Sequence conflict	306	1	H → Y Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9R1I1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6D5371AFB6884AEE	FASTA	388	44,636
	10 20 30 40 50 60 MMLLKKGRLL MFLGSQVIVV ALFIHMSVHR HLSQREESRR PVHVLVLSSW RSGSSFVGQL 70 80 90 100 110 120 FGQHPDVFYL MEPAWHVWMT FTSSTAWKLH MAVRDLLRSV FLCDMSVFDA YMNPGPRKQS 130 140 150 160 170 180 SLFQWEQSRA LCSAPVCDFF PAHEISSPKH CKLLCGQQPF DMVEKACRSH GFVVLKEVRF 190 200 210 220 230 240 LSLQALYPLL TDPSLNLHVV HLVRDPRAVF RSREHTTIEL VVDSHIVLGQ HLETIKEEDQ 250 260 270 280 290 300 PYYAMKIICK SQVDIVKAIQ TLPEALQQRY LFLRYEDLVR APLAQTTRLY KFVGLDFLPH 310 320 330 340 350 360 LQTWVHNVTR GKGMGQHAFH TNARNALNVS QAWRWSLPYE KVSQLQDACG EAMDLLGYLQ 370 380 VRSQQEQGNL SLDLLSSSHI LGQVFREG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel, high endothelial venule-specific sulfotransferase expresses 6-sulfo sialyl Lewis(x), an L-selectin ligand displayed by CD34." Hiraoka N., Petryniak B., Nakayama J., Tsuboi S., Suzuki M., Yeh J.-C., Izawa D., Tanaka T., Miyasaka M., Lowe J.B., Fukuda M. Immunity 11:79-89(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin." Bistrup A., Bhakta S., Lee J.K., Belov Y.Y., Gunn M.D., Zuo F.-R., Huang C.-C., Kannagi R., Rosen S.D., Hemmerich S. J. Cell Biol. 145:899-910(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6. Tissue: Tonsil.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Tongue.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
[5]	"Sulfation of L-selectin ligands by an HEV-restricted sulfotransferase regulates lymphocyte homing to lymph nodes." Hemmerich S., Bistrup A., Singer M.S., van Zante A., Lee J.K., Tsay D., Peters M., Carminati J.L., Brennan T.J., Carver-Moore K., Leviten M., Fuentes M.E., Ruddle N.H., Rosen S.D. Immunity 15:237-247(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"Lymphocyte-HEV interactions in lymph nodes of a sulfotransferase-deficient mouse." van Zante A., Gauguet J.-M., Bistrup A., Tsay D., von Andrian U.H., Rosen S.D. J. Exp. Med. 198:1289-1300(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Detection of a sulfotransferase (HEC-GlcNAc6ST) in high endothelial venules of lymph nodes and in high endothelial venule-like vessels within ectopic lymphoid aggregates: relationship to the MECA-79 epitope." Bistrup A., Tsay D., Shenoy P., Singer M.S., Bangia N., Luther S.A., Cyster J.G., Ruddle N.H., Rosen S.D. Am. J. Pathol. 164:1635-1644(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
[8]	"Core 2 branching beta1,6-N-acetylglucosaminyltransferase and high endothelial venule-restricted sulfotransferase collaboratively control lymphocyte homing." Hiraoka N., Kawashima H., Petryniak B., Nakayama J., Mitoma J., Marth J.D., Lowe J.B., Fukuda M. J. Biol. Chem. 279:3058-3067(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF109155 mRNA. Translation: AAD45579.1. AF131236 Genomic DNA. Translation: AAD33016.1. AK009113 mRNA. Translation: BAB26078.1. BC057886 mRNA. Translation: AAH57886.1.
IPI	IPI00124553.
RefSeq	NP_036128.3. NM_011998.4.
UniGene	Mm.89207.
3D structure databases
ProteinModelPortal	Q9R1I1.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000104845.
PTM databases
PhosphoSite	Q9R1I1.
Proteomic databases
PRIDE	Q9R1I1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000109222; ENSMUSP00000104845; ENSMUSG00000035930.
GeneID	26887.
KEGG	mmu:26887.
UCSC	uc009njt.2. mouse.
Organism-specific databases
CTD	10164.
MGI	MGI:1349479. Chst4.
Phylogenomic databases
eggNOG	NOG80862.
GeneTree	ENSGT00530000062902.
HOGENOM	HOG000261614.
HOVERGEN	HBG050949.
InParanoid	Q9R1I1.
KO	K04746.
OrthoDB	EOG4RXZ09.
Gene expression databases
Bgee	Q9R1I1.
Genevestigator	Q9R1I1.
GermOnline	ENSMUSG00000035930. Mus musculus.
Family and domain databases
InterPro	IPR016469. Carbohydrate_sulfotransferase. IPR000863. Sulfotransferase_dom. [Graphical view]
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
PIRSF	PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
ProtoNet	Search...
Other
NextBio	304707.
SOURCE	Search...

Entry information

Entry name

CHST4_MOUSE

Accession

Primary (citable) accession number: Q9R1I1
Secondary accession number(s): Q9WUE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program