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Q9R1E9 (CTGF_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Connective tissue growth factor
Alternative name(s):
CCN family member 2
Connective tissue growth-related protein
Gene names
Name:Ctgf
Synonyms:Ccn2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes By similarity. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells By similarity. Enhances fibroblast growth factor-induced DNA synthesis By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Sequence similarities

Belongs to the CCN family.

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Contains 1 IGFBP N-terminal domain.

Contains 1 TSP type-1 domain.

Contains 1 VWFC domain.

Ontologies

Keywords
   Biological processCell adhesion
DNA synthesis
   Cellular componentExtracellular matrix
Secreted
   DomainSignal
   LigandHeparin-binding
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

angiogenesis

Traceable author statement PubMed 12193543. Source: RGD

cartilage condensation

Inferred from electronic annotation. Source: Ensembl

cell adhesion

Traceable author statement PubMed 12193543. Source: RGD

cell differentiation

Inferred from electronic annotation. Source: Ensembl

cell migration

Inferred from electronic annotation. Source: Ensembl

cell-matrix adhesion

Inferred from electronic annotation. Source: Ensembl

chondrocyte proliferation

Inferred from electronic annotation. Source: Ensembl

cytosolic calcium ion transport

Inferred from mutant phenotype PubMed 19707545. Source: RGD

extracellular matrix constituent secretion

Inferred from mutant phenotype PubMed 18613219. Source: RGD

fibroblast growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

integrin-mediated signaling pathway

Inferred from electronic annotation. Source: Ensembl

intracellular signal transduction

Inferred from direct assay PubMed 12193543. Source: RGD

lung development

Inferred from expression pattern PubMed 19361631. Source: RGD

negative regulation of cell death

Inferred from mutant phenotype PubMed 20858895. Source: RGD

negative regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

organ senescence

Inferred from expression pattern PubMed 15147944. Source: RGD

ossification

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of G0 to G1 transition

Inferred from mutant phenotype PubMed 18613219. Source: RGD

positive regulation of JNK cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of cardiac muscle contraction

Inferred from mutant phenotype PubMed 19707545. Source: RGD

positive regulation of cell activation

Inferred from mutant phenotype PubMed 18613219. Source: RGD

positive regulation of cell death

Inferred from mutant phenotype PubMed 19625611. Source: RGD

positive regulation of cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 19570811. Source: RGD

positive regulation of collagen biosynthetic process

Inferred from mutant phenotype PubMed 19570811. Source: RGD

positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from mutant phenotype PubMed 19625611. Source: RGD

positive regulation of gene expression

Inferred from mutant phenotype PubMed 19570811. Source: RGD

positive regulation of protein phosphorylation

Inferred from mutant phenotype PubMed 19707545. Source: RGD

positive regulation of stress fiber assembly

Inferred from electronic annotation. Source: Ensembl

reactive oxygen species metabolic process

Inferred from electronic annotation. Source: Ensembl

regulation of cell growth

Inferred from electronic annotation. Source: InterPro

regulation of chondrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

response to amino acid

Inferred from expression pattern PubMed 17250813. Source: RGD

response to anoxia

Inferred from expression pattern PubMed 18929865. Source: RGD

response to estradiol

Inferred from expression pattern PubMed 19509476. Source: RGD

response to fatty acid

Inferred from expression pattern PubMed 19656915. Source: RGD

response to glucose

Inferred from expression pattern PubMed 19625611. Source: RGD

response to mineralocorticoid

Inferred from expression pattern PubMed 19521108. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 19558898. Source: RGD

response to peptide hormone

Inferred from expression pattern PubMed 19667256. Source: RGD

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

cell cortex

Inferred from direct assay PubMed 19359517. Source: RGD

cis-Golgi network

Inferred from direct assay PubMed 11697887. Source: RGD

cytosol

Inferred from direct assay PubMed 19381071. Source: RGD

extracellular space

Inferred from direct assay PubMed 19570811. Source: RGD

perinuclear region of cytoplasm

Inferred from direct assay PubMed 19359517. Source: RGD

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfibronectin binding

Inferred from direct assay PubMed 18167060. Source: RGD

growth factor activity

Inferred from direct assay PubMed 10026205. Source: RGD

heparin binding

Traceable author statement PubMed 12193543. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 347323Connective tissue growth factor
PRO_0000014405

Regions

Domain25 – 9672IGFBP N-terminal
Domain99 – 16567VWFC
Domain196 – 24146TSP type-1
Domain254 – 32875CTCK
Region245 – 347103Heparin-binding By similarity

Amino acid modifications

Disulfide bond254 ↔ 291 By similarity
Disulfide bond271 ↔ 305 By similarity
Disulfide bond282 ↔ 321 By similarity
Disulfide bond285 ↔ 323 By similarity
Disulfide bond290 ↔ 327 By similarity

Experimental info

Sequence conflict351A → R in BAA82125. Ref.2
Sequence conflict941T → P in BAA82125. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9R1E9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: CFBE1A19766B7B16

FASTA34737,756
        10         20         30         40         50         60 
MLASVAGPVS LALVLLLCTR PATGQDCSAQ CQCAAEAAPR CPAGVSLVLD GCGCCRVCAK 

        70         80         90        100        110        120 
QLGELCTERD PCDPHKGLFC DFGSPANRKI GVCTAKDGAP CVFGGSVYRS GESFQSSCKY 

       130        140        150        160        170        180 
QCTCLDGAVG CVPLCSMDVR LPSPDCPFPR RVKLPGKCCE EWVCDEPKDR TVVGPALAAY 

       190        200        210        220        230        240 
RLEDTFGPDP TMMRANCLVQ TTEWSACSKT CGMGISTRVT NDNTFCRLEK QSRLCMVRPC 

       250        260        270        280        290        300 
EADLEENIKK GKKCIRTPKI AKPVKFELSG CTSVKTYRAK FCGVCTDGRC CTPHRTTTLP 

       310        320        330        340 
VEFKCPDGEI MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA 

« Hide

References

[1]"Cloning the full-length cDNA for rat connective tissue growth factor: implications for skeletal development."
Xu J., Smock S.L., Safadi F.F., Rosenzweig A.B., Odgren P.R., Marks S.C. Jr., Owen T.A., Popoff S.N.
J. Cell. Biochem. 77:103-115(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo."
Shimo T., Nakanishi T., Nishida T., Asano M., Kanyama M., Kuboki T., Tamatani T., Tezuka K., Takemura M., Matsumura T., Takigawa M.
J. Biochem. 126:137-145(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Connective tissue growth related gene."
Ma L.N., Zou Y.L.
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF120275 mRNA. Translation: AAD39132.1.
AB023068 mRNA. Translation: BAA82125.1.
AY596447 Transcribed RNA. Translation: AAT08023.1.
RefSeqNP_071602.1. NM_022266.2.
UniGeneRn.17145.

3D structure databases

ProteinModelPortalQ9R1E9.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9R1E9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000020528; ENSRNOP00000020528; ENSRNOG00000015036.
GeneID64032.
KEGGrno:64032.

Organism-specific databases

CTD1490.
RGD621392. Ctgf.

Phylogenomic databases

eggNOGNOG72934.
GeneTreeENSGT00750000117442.
HOGENOMHOG000231462.
HOVERGENHBG000635.
InParanoidQ9R1E9.
KOK06827.
OMAKTYRAKF.
OrthoDBEOG7N8ZXB.
PhylomeDBQ9R1E9.
TreeFamTF326070.

Gene expression databases

GenevestigatorQ9R1E9.

Family and domain databases

InterProIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612658.
PROQ9R1E9.

Entry information

Entry nameCTGF_RAT
AccessionPrimary (citable) accession number: Q9R1E9
Secondary accession number(s): Q53YJ0, Q9WVS1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families