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Protein

Connective tissue growth factor

Gene

Ctgf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).By similarity

GO - Molecular functioni

  1. fibronectin binding Source: RGD
  2. growth factor activity Source: RGD
  3. heparin binding Source: RGD
  4. integrin binding Source: GO_Central

GO - Biological processi

  1. angiogenesis Source: RGD
  2. cartilage condensation Source: Ensembl
  3. cell adhesion Source: RGD
  4. cell-cell signaling Source: GO_Central
  5. cell differentiation Source: Ensembl
  6. cell-matrix adhesion Source: Ensembl
  7. cell migration Source: Ensembl
  8. chondrocyte proliferation Source: Ensembl
  9. cytosolic calcium ion transport Source: RGD
  10. DNA biosynthetic process Source: UniProtKB-KW
  11. extracellular matrix constituent secretion Source: RGD
  12. fibroblast growth factor receptor signaling pathway Source: Ensembl
  13. integrin-mediated signaling pathway Source: Ensembl
  14. intracellular signal transduction Source: RGD
  15. lung development Source: RGD
  16. negative regulation of cell death Source: RGD
  17. negative regulation of gene expression Source: Ensembl
  18. organ senescence Source: RGD
  19. ossification Source: Ensembl
  20. positive regulation of cardiac muscle contraction Source: RGD
  21. positive regulation of cell activation Source: RGD
  22. positive regulation of cell death Source: RGD
  23. positive regulation of cell differentiation Source: Ensembl
  24. positive regulation of cell proliferation Source: RGD
  25. positive regulation of collagen biosynthetic process Source: RGD
  26. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
  27. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  28. positive regulation of G0 to G1 transition Source: RGD
  29. positive regulation of gene expression Source: RGD
  30. positive regulation of JNK cascade Source: Ensembl
  31. positive regulation of protein phosphorylation Source: RGD
  32. positive regulation of stress fiber assembly Source: Ensembl
  33. reactive oxygen species metabolic process Source: Ensembl
  34. regulation of cell growth Source: InterPro
  35. regulation of chondrocyte differentiation Source: Ensembl
  36. response to amino acid Source: RGD
  37. response to anoxia Source: RGD
  38. response to estradiol Source: RGD
  39. response to fatty acid Source: RGD
  40. response to glucose Source: RGD
  41. response to mineralocorticoid Source: RGD
  42. response to organic cyclic compound Source: RGD
  43. response to peptide hormone Source: RGD
  44. tissue homeostasis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, DNA synthesis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_196408. PPARA activates gene expression.
REACT_198793. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Connective tissue growth factor
Alternative name(s):
CCN family member 2
Connective tissue growth-related protein
Gene namesi
Name:Ctgf
Synonyms:Ccn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi621392. Ctgf.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: RGD
  2. cis-Golgi network Source: RGD
  3. cytosol Source: RGD
  4. extracellular space Source: RGD
  5. Golgi apparatus Source: Ensembl
  6. perinuclear region of cytoplasm Source: RGD
  7. proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 347323Connective tissue growth factorPRO_0000014405Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi254 ↔ 291By similarity
Disulfide bondi271 ↔ 305By similarity
Disulfide bondi282 ↔ 321By similarity
Disulfide bondi285 ↔ 323By similarity
Disulfide bondi290 ↔ 327By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9R1E9.

Expressioni

Gene expression databases

ExpressionAtlasiQ9R1E9. baseline and differential.
GenevestigatoriQ9R1E9.

Structurei

3D structure databases

ProteinModelPortaliQ9R1E9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 9672IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini99 – 16567VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini196 – 24146TSP type-1PROSITE-ProRule annotationAdd
BLAST
Domaini254 – 32875CTCKPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni245 – 347103Heparin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CCN family.Curated
Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG72934.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiQ9R1E9.
KOiK06827.
OMAiTYRAKFC.
OrthoDBiEOG7N8ZXB.
PhylomeDBiQ9R1E9.
TreeFamiTF326070.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R1E9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASVAGPVS LALVLLLCTR PATGQDCSAQ CQCAAEAAPR CPAGVSLVLD
60 70 80 90 100
GCGCCRVCAK QLGELCTERD PCDPHKGLFC DFGSPANRKI GVCTAKDGAP
110 120 130 140 150
CVFGGSVYRS GESFQSSCKY QCTCLDGAVG CVPLCSMDVR LPSPDCPFPR
160 170 180 190 200
RVKLPGKCCE EWVCDEPKDR TVVGPALAAY RLEDTFGPDP TMMRANCLVQ
210 220 230 240 250
TTEWSACSKT CGMGISTRVT NDNTFCRLEK QSRLCMVRPC EADLEENIKK
260 270 280 290 300
GKKCIRTPKI AKPVKFELSG CTSVKTYRAK FCGVCTDGRC CTPHRTTTLP
310 320 330 340
VEFKCPDGEI MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA
Length:347
Mass (Da):37,756
Last modified:May 1, 2000 - v1
Checksum:iCFBE1A19766B7B16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351A → R in BAA82125 (PubMed:10393331).Curated
Sequence conflicti94 – 941T → P in BAA82125 (PubMed:10393331).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF120275 mRNA. Translation: AAD39132.1.
AB023068 mRNA. Translation: BAA82125.1.
AY596447 Transcribed RNA. Translation: AAT08023.1.
RefSeqiNP_071602.1. NM_022266.2.
UniGeneiRn.17145.

Genome annotation databases

EnsembliENSRNOT00000020528; ENSRNOP00000020528; ENSRNOG00000015036.
GeneIDi64032.
KEGGirno:64032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF120275 mRNA. Translation: AAD39132.1.
AB023068 mRNA. Translation: BAA82125.1.
AY596447 Transcribed RNA. Translation: AAT08023.1.
RefSeqiNP_071602.1. NM_022266.2.
UniGeneiRn.17145.

3D structure databases

ProteinModelPortaliQ9R1E9.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ9R1E9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020528; ENSRNOP00000020528; ENSRNOG00000015036.
GeneIDi64032.
KEGGirno:64032.

Organism-specific databases

CTDi1490.
RGDi621392. Ctgf.

Phylogenomic databases

eggNOGiNOG72934.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiQ9R1E9.
KOiK06827.
OMAiTYRAKFC.
OrthoDBiEOG7N8ZXB.
PhylomeDBiQ9R1E9.
TreeFamiTF326070.

Enzyme and pathway databases

ReactomeiREACT_196408. PPARA activates gene expression.
REACT_198793. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Miscellaneous databases

NextBioi612658.
PROiQ9R1E9.

Gene expression databases

ExpressionAtlasiQ9R1E9. baseline and differential.
GenevestigatoriQ9R1E9.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning the full-length cDNA for rat connective tissue growth factor: implications for skeletal development."
    Xu J., Smock S.L., Safadi F.F., Rosenzweig A.B., Odgren P.R., Marks S.C. Jr., Owen T.A., Popoff S.N.
    J. Cell. Biochem. 77:103-115(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo."
    Shimo T., Nakanishi T., Nishida T., Asano M., Kanyama M., Kuboki T., Tamatani T., Tezuka K., Takemura M., Matsumura T., Takigawa M.
    J. Biochem. 126:137-145(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Connective tissue growth related gene."
    Ma L.N., Zou Y.L.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.

Entry informationi

Entry nameiCTGF_RAT
AccessioniPrimary (citable) accession number: Q9R1E9
Secondary accession number(s): Q53YJ0, Q9WVS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.