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Protein

Pre-mRNA-processing factor 40 homolog A

Gene

Prpf40a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: MGI
  • proline-rich region binding Source: MGI
  • RNA binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 40 homolog A
Alternative name(s):
Formin-binding protein 11
Short name:
FBP-11
Formin-binding protein 3
Gene namesi
Name:Prpf40a
Synonyms:Fbp11, Fnbp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1860512. Prpf40a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nuclear matrix Source: UniProtKB
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • U1 snRNP Source: GO_Central
  • U2-type prespliceosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760861 – 953Pre-mRNA-processing factor 40 homolog AAdd BLAST953

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Omega-N-methylarginineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei151PhosphoserineBy similarity1
Modified residuei196N6-acetyllysineCombined sources1
Cross-linki241Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei341PhosphothreonineBy similarity1
Modified residuei369PhosphothreonineBy similarity1
Modified residuei719PhosphoserineBy similarity1
Modified residuei783PhosphoserineBy similarity1
Modified residuei879PhosphoserineBy similarity1
Modified residuei881PhosphoserineBy similarity1
Modified residuei884PhosphoserineBy similarity1
Modified residuei928PhosphothreonineCombined sources1
Modified residuei929PhosphoserineCombined sources1
Modified residuei931PhosphoserineCombined sources1
Modified residuei934PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9R1C7.
PaxDbiQ9R1C7.
PeptideAtlasiQ9R1C7.
PRIDEiQ9R1C7.

PTM databases

iPTMnetiQ9R1C7.
PhosphoSitePlusiQ9R1C7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000061136.
ExpressionAtlasiQ9R1C7. baseline and differential.
GenevisibleiQ9R1C7. MM.

Interactioni

Subunit structurei

Interacts with the N-terminus of HTT and with the phosphorylated C-terminal domain of POLR2A (By similarity). Interacts with AKAP8L, SF1, SRPK1, CARD8, ATBF1 and MECP2. Interacts through the WW domains with formin proline-rich regions and with WASL/N-WASP.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AKAP8LQ9ULX63EBI-645554,EBI-357530From a different organism.

GO - Molecular functioni

  • proline-rich region binding Source: MGI

Protein-protein interaction databases

BioGridi207833. 6 interactors.
IntActiQ9R1C7. 5 interactors.
MINTiMINT-1525232.
STRINGi10090.ENSMUSP00000075655.

Structurei

3D structure databases

ProteinModelPortaliQ9R1C7.
SMRiQ9R1C7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 173WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini181 – 214WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini389 – 443FF 1Add BLAST55
Domaini456 – 510FF 2Add BLAST55
Domaini523 – 583FF 3Add BLAST61
Domaini603 – 663FF 4Add BLAST61
Domaini668 – 723FF 5Add BLAST56
Domaini738 – 795FF 6Add BLAST58

Domaini

The WW domains are essential for localization to nuclear speckles.

Sequence similaritiesi

Belongs to the PRPF40 family.Curated
Contains 6 FF domains.Curated
Contains 2 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0152. Eukaryota.
COG5104. LUCA.
GeneTreeiENSGT00820000127014.
HOGENOMiHOG000231802.
HOVERGENiHBG059634.
InParanoidiQ9R1C7.
KOiK12821.
OMAiYSKQEEC.
OrthoDBiEOG091G06F8.
PhylomeDBiQ9R1C7.
TreeFamiTF318732.

Family and domain databases

Gene3Di1.10.10.440. 5 hits.
InterProiIPR002713. FF_domain.
IPR001202. WW_dom.
[Graphical view]
PfamiPF01846. FF. 5 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00441. FF. 5 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 2 hits.
SSF81698. SSF81698. 5 hits.
PROSITEiPS51676. FF. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R1C7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPGTGAERG GLMVSEMESQ PPSRGPGDGE RRLSGSNLCS SSWVSADGFL
60 70 80 90 100
RRRPSMGHPG MHYAPMGMHP MGQRANMPPV PHGMMPQMMP PMGGPPMGQM
110 120 130 140 150
PGMMSSVMSG MMMSHMSQAS MQPALPPGVN SMDVAAGAAS GAKSMWTEHK
160 170 180 190 200
SPDGRTYYYN TETKQSTWEK PDDLKTPAEQ LLSKCPWKEY KSDSGKPYYY
210 220 230 240 250
NSQTKESRWA KPKELEDLEG YQNTIVAGGL ITKSNLHAMI KAEESSKQEE
260 270 280 290 300
CTTASTAPVP TTEIPTTMST MAAAEAAAAV VAAAAAAAAA ANANTSTTPT
310 320 330 340 350
NTVGSVPVAP EPEVTSIVAT AVDNENTVTV STEEQAQLAN TTAIQDLSGD
360 370 380 390 400
ISSNTGEEPA KQETVSDFTP KKEEEESQPA KKTYTWNTKE EAKQAFKELL
410 420 430 440 450
KEKRVPSNAS WEQAMKMIIN DPRYSALAKL SEKKQAFNAY KVQTEKEEKE
460 470 480 490 500
EARSKYKEAK ESFQRFLENH EKMTSTTRYK KAEQMFGEME VWNAISERDR
510 520 530 540 550
LEIYEDVLFF LSKKEKEQAK QLRKRNWEAL KNILDNMANV TYSTTWSEAQ
560 570 580 590 600
QYLMDNPTFA EDEELQNMDK EDALICFEEH IRALEKEEEE EKQKTLLRER
610 620 630 640 650
RRQRKNRESF QIFLDELHEH GQLHSMSSWM ELYPTISSDI RFTNMLGQPG
660 670 680 690 700
STALDLFKFY VEDLKARYHD EKKIIKDILK DKGFVVEVNT TFEDFVAIIS
710 720 730 740 750
STKRSTTLDA GNIKLAFNSL LEKAEARERE REKEEARKMK RKESAFKSML
760 770 780 790 800
KQATPPIELD AVWEDIRERF VKEPAFEDIT LESERKRIFK DFMHVLEHEC
810 820 830 840 850
QHHHSKNKKH SKKSKKHHRK RSRSRSGSES DDDDSHSKKK RQRSESHSAS
860 870 880 890 900
ERSSSAESER SYKKSKKHKK KSKKRRHKSD SPESDTEREK DKKEKDRDSE
910 920 930 940 950
KDRSRQRSES KHKSPKKKTG KDSGNWDTSG SELSEGELEK RRRTLLEQLD

DDQ
Length:953
Mass (Da):108,481
Last modified:May 1, 2000 - v1
Checksum:i3C627AB7404D2285
GO
Isoform 2 (identifier: Q9R1C7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-55: Missing.

Note: No experimental confirmation available.
Show »
Length:911
Mass (Da):103,963
Checksum:i7BE2E454CC5365CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti334E → A in BAC40061 (PubMed:16141072).Curated1
Sequence conflicti355T → I in BAC40061 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00804914 – 55Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135439 mRNA. Translation: AAD39463.1.
AK041205 mRNA. Translation: BAC30863.1.
AK051375 mRNA. Translation: BAC34617.1.
AK087963 mRNA. Translation: BAC40061.2.
U40747 mRNA. Translation: AAC52475.1.
CCDSiCCDS16038.1. [Q9R1C7-1]
PIRiS64713.
RefSeqiNP_061255.1. NM_018785.2. [Q9R1C7-1]
UniGeneiMm.257474.
Mm.392945.
Mm.393219.

Genome annotation databases

EnsembliENSMUST00000076313; ENSMUSP00000075655; ENSMUSG00000061136. [Q9R1C7-1]
ENSMUST00000210789; ENSMUSP00000147599; ENSMUSG00000061136. [Q9R1C7-2]
GeneIDi56194.
KEGGimmu:56194.
UCSCiuc008jrk.2. mouse. [Q9R1C7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135439 mRNA. Translation: AAD39463.1.
AK041205 mRNA. Translation: BAC30863.1.
AK051375 mRNA. Translation: BAC34617.1.
AK087963 mRNA. Translation: BAC40061.2.
U40747 mRNA. Translation: AAC52475.1.
CCDSiCCDS16038.1. [Q9R1C7-1]
PIRiS64713.
RefSeqiNP_061255.1. NM_018785.2. [Q9R1C7-1]
UniGeneiMm.257474.
Mm.392945.
Mm.393219.

3D structure databases

ProteinModelPortaliQ9R1C7.
SMRiQ9R1C7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207833. 6 interactors.
IntActiQ9R1C7. 5 interactors.
MINTiMINT-1525232.
STRINGi10090.ENSMUSP00000075655.

PTM databases

iPTMnetiQ9R1C7.
PhosphoSitePlusiQ9R1C7.

Proteomic databases

EPDiQ9R1C7.
PaxDbiQ9R1C7.
PeptideAtlasiQ9R1C7.
PRIDEiQ9R1C7.

Protocols and materials databases

DNASUi56194.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076313; ENSMUSP00000075655; ENSMUSG00000061136. [Q9R1C7-1]
ENSMUST00000210789; ENSMUSP00000147599; ENSMUSG00000061136. [Q9R1C7-2]
GeneIDi56194.
KEGGimmu:56194.
UCSCiuc008jrk.2. mouse. [Q9R1C7-1]

Organism-specific databases

CTDi55660.
MGIiMGI:1860512. Prpf40a.

Phylogenomic databases

eggNOGiKOG0152. Eukaryota.
COG5104. LUCA.
GeneTreeiENSGT00820000127014.
HOGENOMiHOG000231802.
HOVERGENiHBG059634.
InParanoidiQ9R1C7.
KOiK12821.
OMAiYSKQEEC.
OrthoDBiEOG091G06F8.
PhylomeDBiQ9R1C7.
TreeFamiTF318732.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ9R1C7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061136.
ExpressionAtlasiQ9R1C7. baseline and differential.
GenevisibleiQ9R1C7. MM.

Family and domain databases

Gene3Di1.10.10.440. 5 hits.
InterProiIPR002713. FF_domain.
IPR001202. WW_dom.
[Graphical view]
PfamiPF01846. FF. 5 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00441. FF. 5 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF51045. SSF51045. 2 hits.
SSF81698. SSF81698. 5 hits.
PROSITEiPS51676. FF. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPR40A_MOUSE
AccessioniPrimary (citable) accession number: Q9R1C7
Secondary accession number(s): Q61049
, Q8BQ76, Q8BRW4, Q8C2U1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.