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Q9R1C7

- PR40A_MOUSE

UniProt

Q9R1C7 - PR40A_MOUSE

Protein

Pre-mRNA-processing factor 40 homolog A

Gene

Prpf40a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.1 Publication

    GO - Molecular functioni

    1. proline-rich region binding Source: MGI
    2. protein binding Source: MGI

    GO - Biological processi

    1. cell migration Source: UniProtKB
    2. cytoskeleton organization Source: UniProtKB
    3. mRNA processing Source: UniProtKB-KW
    4. regulation of cell shape Source: UniProtKB
    5. regulation of cytokinesis Source: UniProtKB
    6. RNA splicing Source: UniProtKB-KW

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-processing factor 40 homolog A
    Alternative name(s):
    Formin-binding protein 11
    Short name:
    FBP-11
    Formin-binding protein 3
    Gene namesi
    Name:Prpf40a
    Synonyms:Fbp11, Fnbp3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1860512. Prpf40a.

    Subcellular locationi

    Nucleus speckle. Nucleus matrix
    Note: Colocalizes with AKAP8L in the nuclear matrix.

    GO - Cellular componenti

    1. nuclear matrix Source: UniProtKB
    2. nuclear speck Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Pre-mRNA-processing factor 40 homolog APRO_0000076086Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei151 – 1511PhosphoserineBy similarity
    Modified residuei196 – 1961N6-acetyllysine1 Publication
    Modified residuei369 – 3691PhosphothreonineBy similarity
    Modified residuei879 – 8791PhosphoserineBy similarity
    Modified residuei881 – 8811PhosphoserineBy similarity
    Modified residuei884 – 8841PhosphoserineBy similarity
    Modified residuei928 – 9281Phosphothreonine1 Publication
    Modified residuei929 – 9291Phosphoserine1 Publication
    Modified residuei931 – 9311Phosphoserine1 Publication
    Modified residuei934 – 9341Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9R1C7.
    PaxDbiQ9R1C7.
    PRIDEiQ9R1C7.

    PTM databases

    PhosphoSiteiQ9R1C7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9R1C7.
    BgeeiQ9R1C7.
    GenevestigatoriQ9R1C7.

    Interactioni

    Subunit structurei

    Interacts with the N-terminus of HTT and with the phosphorylated C-terminal domain of POLR2A By similarity. Interacts with AKAP8L, SF1, SRPK1, CARD8, ATBF1 and MECP2. Interacts through the WW domains with formin proline-rich regions and with WASL/N-WASP.By similarity4 Publications

    Protein-protein interaction databases

    BioGridi207833. 5 interactions.
    IntActiQ9R1C7. 4 interactions.
    MINTiMINT-1525232.
    STRINGi10090.ENSMUSP00000075655.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R1C7.
    SMRiQ9R1C7. Positions 133-222, 378-446, 739-802.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini140 – 17334WW 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini181 – 21434WW 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini389 – 44355FF 1Add
    BLAST
    Domaini456 – 51055FF 2Add
    BLAST
    Domaini523 – 58361FF 3Add
    BLAST
    Domaini603 – 66361FF 4Add
    BLAST
    Domaini668 – 72356FF 5Add
    BLAST
    Domaini738 – 79558FF 6Add
    BLAST

    Domaini

    The WW domains are essential for localization to nuclear speckles.

    Sequence similaritiesi

    Belongs to the PRPF40 family.Curated
    Contains 6 FF domains.Curated
    Contains 2 WW domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5104.
    GeneTreeiENSGT00740000115182.
    HOGENOMiHOG000231802.
    HOVERGENiHBG059634.
    InParanoidiQ9R1C7.
    KOiK12821.
    OMAiERGGLMM.
    OrthoDBiEOG77Q4X2.
    PhylomeDBiQ9R1C7.
    TreeFamiTF318732.

    Family and domain databases

    Gene3Di1.10.10.440. 4 hits.
    InterProiIPR002713. FF_domain.
    IPR001202. WW_dom.
    [Graphical view]
    PfamiPF01846. FF. 5 hits.
    PF00397. WW. 2 hits.
    [Graphical view]
    SMARTiSM00441. FF. 5 hits.
    SM00456. WW. 2 hits.
    [Graphical view]
    SUPFAMiSSF51045. SSF51045. 2 hits.
    SSF81698. SSF81698. 5 hits.
    PROSITEiPS51676. FF. 6 hits.
    PS01159. WW_DOMAIN_1. 2 hits.
    PS50020. WW_DOMAIN_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9R1C7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRPGTGAERG GLMVSEMESQ PPSRGPGDGE RRLSGSNLCS SSWVSADGFL    50
    RRRPSMGHPG MHYAPMGMHP MGQRANMPPV PHGMMPQMMP PMGGPPMGQM 100
    PGMMSSVMSG MMMSHMSQAS MQPALPPGVN SMDVAAGAAS GAKSMWTEHK 150
    SPDGRTYYYN TETKQSTWEK PDDLKTPAEQ LLSKCPWKEY KSDSGKPYYY 200
    NSQTKESRWA KPKELEDLEG YQNTIVAGGL ITKSNLHAMI KAEESSKQEE 250
    CTTASTAPVP TTEIPTTMST MAAAEAAAAV VAAAAAAAAA ANANTSTTPT 300
    NTVGSVPVAP EPEVTSIVAT AVDNENTVTV STEEQAQLAN TTAIQDLSGD 350
    ISSNTGEEPA KQETVSDFTP KKEEEESQPA KKTYTWNTKE EAKQAFKELL 400
    KEKRVPSNAS WEQAMKMIIN DPRYSALAKL SEKKQAFNAY KVQTEKEEKE 450
    EARSKYKEAK ESFQRFLENH EKMTSTTRYK KAEQMFGEME VWNAISERDR 500
    LEIYEDVLFF LSKKEKEQAK QLRKRNWEAL KNILDNMANV TYSTTWSEAQ 550
    QYLMDNPTFA EDEELQNMDK EDALICFEEH IRALEKEEEE EKQKTLLRER 600
    RRQRKNRESF QIFLDELHEH GQLHSMSSWM ELYPTISSDI RFTNMLGQPG 650
    STALDLFKFY VEDLKARYHD EKKIIKDILK DKGFVVEVNT TFEDFVAIIS 700
    STKRSTTLDA GNIKLAFNSL LEKAEARERE REKEEARKMK RKESAFKSML 750
    KQATPPIELD AVWEDIRERF VKEPAFEDIT LESERKRIFK DFMHVLEHEC 800
    QHHHSKNKKH SKKSKKHHRK RSRSRSGSES DDDDSHSKKK RQRSESHSAS 850
    ERSSSAESER SYKKSKKHKK KSKKRRHKSD SPESDTEREK DKKEKDRDSE 900
    KDRSRQRSES KHKSPKKKTG KDSGNWDTSG SELSEGELEK RRRTLLEQLD 950
    DDQ 953
    Length:953
    Mass (Da):108,481
    Last modified:May 1, 2000 - v1
    Checksum:i3C627AB7404D2285
    GO
    Isoform 2 (identifier: Q9R1C7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         14-55: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:911
    Mass (Da):103,963
    Checksum:i7BE2E454CC5365CA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti334 – 3341E → A in BAC40061. (PubMed:16141072)Curated
    Sequence conflicti355 – 3551T → I in BAC40061. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei14 – 5542Missing in isoform 2. 1 PublicationVSP_008049Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF135439 mRNA. Translation: AAD39463.1.
    AK041205 mRNA. Translation: BAC30863.1.
    AK051375 mRNA. Translation: BAC34617.1.
    AK087963 mRNA. Translation: BAC40061.2.
    U40747 mRNA. Translation: AAC52475.1.
    CCDSiCCDS16038.1. [Q9R1C7-1]
    PIRiS64713.
    RefSeqiNP_061255.1. NM_018785.2. [Q9R1C7-1]
    UniGeneiMm.257474.
    Mm.392945.
    Mm.393219.

    Genome annotation databases

    EnsembliENSMUST00000076313; ENSMUSP00000075655; ENSMUSG00000061136. [Q9R1C7-1]
    GeneIDi56194.
    KEGGimmu:56194.
    UCSCiuc008jrk.2. mouse. [Q9R1C7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF135439 mRNA. Translation: AAD39463.1 .
    AK041205 mRNA. Translation: BAC30863.1 .
    AK051375 mRNA. Translation: BAC34617.1 .
    AK087963 mRNA. Translation: BAC40061.2 .
    U40747 mRNA. Translation: AAC52475.1 .
    CCDSi CCDS16038.1. [Q9R1C7-1 ]
    PIRi S64713.
    RefSeqi NP_061255.1. NM_018785.2. [Q9R1C7-1 ]
    UniGenei Mm.257474.
    Mm.392945.
    Mm.393219.

    3D structure databases

    ProteinModelPortali Q9R1C7.
    SMRi Q9R1C7. Positions 133-222, 378-446, 739-802.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207833. 5 interactions.
    IntActi Q9R1C7. 4 interactions.
    MINTi MINT-1525232.
    STRINGi 10090.ENSMUSP00000075655.

    PTM databases

    PhosphoSitei Q9R1C7.

    Proteomic databases

    MaxQBi Q9R1C7.
    PaxDbi Q9R1C7.
    PRIDEi Q9R1C7.

    Protocols and materials databases

    DNASUi 56194.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000076313 ; ENSMUSP00000075655 ; ENSMUSG00000061136 . [Q9R1C7-1 ]
    GeneIDi 56194.
    KEGGi mmu:56194.
    UCSCi uc008jrk.2. mouse. [Q9R1C7-1 ]

    Organism-specific databases

    CTDi 55660.
    MGIi MGI:1860512. Prpf40a.

    Phylogenomic databases

    eggNOGi COG5104.
    GeneTreei ENSGT00740000115182.
    HOGENOMi HOG000231802.
    HOVERGENi HBG059634.
    InParanoidi Q9R1C7.
    KOi K12821.
    OMAi ERGGLMM.
    OrthoDBi EOG77Q4X2.
    PhylomeDBi Q9R1C7.
    TreeFami TF318732.

    Miscellaneous databases

    NextBioi 312006.
    PROi Q9R1C7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R1C7.
    Bgeei Q9R1C7.
    Genevestigatori Q9R1C7.

    Family and domain databases

    Gene3Di 1.10.10.440. 4 hits.
    InterProi IPR002713. FF_domain.
    IPR001202. WW_dom.
    [Graphical view ]
    Pfami PF01846. FF. 5 hits.
    PF00397. WW. 2 hits.
    [Graphical view ]
    SMARTi SM00441. FF. 5 hits.
    SM00456. WW. 2 hits.
    [Graphical view ]
    SUPFAMi SSF51045. SSF51045. 2 hits.
    SSF81698. SSF81698. 5 hits.
    PROSITEi PS51676. FF. 6 hits.
    PS01159. WW_DOMAIN_1. 2 hits.
    PS50020. WW_DOMAIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "FBP11, a mammalian ortholog of the essential yeast splicing factor PRP40."
      Bedford M.T., Das R., Reed R., Leder P.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-380 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 647-953.
      Strain: C57BL/6J and NOD.
      Tissue: Aorta, Embryonic spinal ganglion, Thymus and Vein.
    3. "Formin binding proteins bear WWP/WW domains that bind proline-rich peptides and functionally resemble SH3 domains."
      Chan D.C., Bedford M.T., Leder P.
      EMBO J. 15:1045-1054(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 146-212, INTERACTION WITH FORMIN.
      Strain: FVB.
    4. "FBP WW domains and the Abl SH3 domain bind to a specific class of proline-rich ligands."
      Bedford M.T., Chan D.C., Leder P.
      EMBO J. 16:2376-2383(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SF1; SRPK1; CARD8; ATBF1 AND MECP2.
    5. "FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation."
      Mizutani K., Suetsugu S., Takenawa T.
      Biochem. Biophys. Res. Commun. 313:468-474(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL.
    6. "Interaction of the nuclear matrix protein NAKAP with HypA and huntingtin: implications for nuclear toxicity in Huntington's disease pathogenesis."
      Sayer J.A., Manczak M., Akileswaran L., Reddy P.H., Coghlan V.M.
      NeuroMolecular Med. 7:297-310(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AKAP8L, SUBCELLULAR LOCATION.
    7. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-928; SER-929; SER-931 AND SER-934, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-196, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiPR40A_MOUSE
    AccessioniPrimary (citable) accession number: Q9R1C7
    Secondary accession number(s): Q61049
    , Q8BQ76, Q8BRW4, Q8C2U1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 22, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3