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Q9R1C7 (PR40A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-processing factor 40 homolog A
Alternative name(s):
Formin-binding protein 11
Short name=FBP-11
Formin-binding protein 3
Gene names
Name:Prpf40a
Synonyms:Fbp11, Fnbp3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length953 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing. Ref.5

Subunit structure

Interacts with the N-terminus of HTT and with the phosphorylated C-terminal domain of POLR2A By similarity. Interacts with AKAP8L, SF1, SRPK1, CARD8, ATBF1 and MECP2. Interacts through the WW domains with formin proline-rich regions and with WASL/N-WASP. Ref.3 Ref.4 Ref.5 Ref.6

Subcellular location

Nucleus speckle. Nucleus matrix. Note: Colocalizes with AKAP8L in the nuclear matrix. Ref.5 Ref.6

Domain

The WW domains are essential for localization to nuclear speckles.

Sequence similarities

Belongs to the PRPF40 family.

Contains 6 FF domains.

Contains 2 WW domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9R1C7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9R1C7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     14-55: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 953953Pre-mRNA-processing factor 40 homolog A
PRO_0000076086

Regions

Domain140 – 17334WW 1
Domain181 – 21434WW 2
Domain389 – 44355FF 1
Domain456 – 51055FF 2
Domain523 – 58361FF 3
Domain603 – 66361FF 4
Domain668 – 72356FF 5
Domain738 – 79558FF 6

Amino acid modifications

Modified residue1511Phosphoserine By similarity
Modified residue1961N6-acetyllysine Ref.8
Modified residue3691Phosphothreonine By similarity
Modified residue8791Phosphoserine By similarity
Modified residue8811Phosphoserine By similarity
Modified residue8841Phosphoserine By similarity
Modified residue9281Phosphothreonine Ref.7
Modified residue9291Phosphoserine Ref.7
Modified residue9311Phosphoserine Ref.7
Modified residue9341Phosphoserine Ref.7

Natural variations

Alternative sequence14 – 5542Missing in isoform 2.
VSP_008049

Experimental info

Sequence conflict3341E → A in BAC40061. Ref.2
Sequence conflict3551T → I in BAC40061. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 3C627AB7404D2285

FASTA953108,481
        10         20         30         40         50         60 
MRPGTGAERG GLMVSEMESQ PPSRGPGDGE RRLSGSNLCS SSWVSADGFL RRRPSMGHPG 

        70         80         90        100        110        120 
MHYAPMGMHP MGQRANMPPV PHGMMPQMMP PMGGPPMGQM PGMMSSVMSG MMMSHMSQAS 

       130        140        150        160        170        180 
MQPALPPGVN SMDVAAGAAS GAKSMWTEHK SPDGRTYYYN TETKQSTWEK PDDLKTPAEQ 

       190        200        210        220        230        240 
LLSKCPWKEY KSDSGKPYYY NSQTKESRWA KPKELEDLEG YQNTIVAGGL ITKSNLHAMI 

       250        260        270        280        290        300 
KAEESSKQEE CTTASTAPVP TTEIPTTMST MAAAEAAAAV VAAAAAAAAA ANANTSTTPT 

       310        320        330        340        350        360 
NTVGSVPVAP EPEVTSIVAT AVDNENTVTV STEEQAQLAN TTAIQDLSGD ISSNTGEEPA 

       370        380        390        400        410        420 
KQETVSDFTP KKEEEESQPA KKTYTWNTKE EAKQAFKELL KEKRVPSNAS WEQAMKMIIN 

       430        440        450        460        470        480 
DPRYSALAKL SEKKQAFNAY KVQTEKEEKE EARSKYKEAK ESFQRFLENH EKMTSTTRYK 

       490        500        510        520        530        540 
KAEQMFGEME VWNAISERDR LEIYEDVLFF LSKKEKEQAK QLRKRNWEAL KNILDNMANV 

       550        560        570        580        590        600 
TYSTTWSEAQ QYLMDNPTFA EDEELQNMDK EDALICFEEH IRALEKEEEE EKQKTLLRER 

       610        620        630        640        650        660 
RRQRKNRESF QIFLDELHEH GQLHSMSSWM ELYPTISSDI RFTNMLGQPG STALDLFKFY 

       670        680        690        700        710        720 
VEDLKARYHD EKKIIKDILK DKGFVVEVNT TFEDFVAIIS STKRSTTLDA GNIKLAFNSL 

       730        740        750        760        770        780 
LEKAEARERE REKEEARKMK RKESAFKSML KQATPPIELD AVWEDIRERF VKEPAFEDIT 

       790        800        810        820        830        840 
LESERKRIFK DFMHVLEHEC QHHHSKNKKH SKKSKKHHRK RSRSRSGSES DDDDSHSKKK 

       850        860        870        880        890        900 
RQRSESHSAS ERSSSAESER SYKKSKKHKK KSKKRRHKSD SPESDTEREK DKKEKDRDSE 

       910        920        930        940        950 
KDRSRQRSES KHKSPKKKTG KDSGNWDTSG SELSEGELEK RRRTLLEQLD DDQ 

« Hide

Isoform 2 [UniParc].

Checksum: 7BE2E454CC5365CA
Show »

FASTA911103,963

References

« Hide 'large scale' references
[1]"FBP11, a mammalian ortholog of the essential yeast splicing factor PRP40."
Bedford M.T., Das R., Reed R., Leder P.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-380 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 647-953.
Strain: C57BL/6J and NOD.
Tissue: Aorta, Embryonic spinal ganglion, Thymus and Vein.
[3]"Formin binding proteins bear WWP/WW domains that bind proline-rich peptides and functionally resemble SH3 domains."
Chan D.C., Bedford M.T., Leder P.
EMBO J. 15:1045-1054(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 146-212, INTERACTION WITH FORMIN.
Strain: FVB.
[4]"FBP WW domains and the Abl SH3 domain bind to a specific class of proline-rich ligands."
Bedford M.T., Chan D.C., Leder P.
EMBO J. 16:2376-2383(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SF1; SRPK1; CARD8; ATBF1 AND MECP2.
[5]"FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation."
Mizutani K., Suetsugu S., Takenawa T.
Biochem. Biophys. Res. Commun. 313:468-474(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL.
[6]"Interaction of the nuclear matrix protein NAKAP with HypA and huntingtin: implications for nuclear toxicity in Huntington's disease pathogenesis."
Sayer J.A., Manczak M., Akileswaran L., Reddy P.H., Coghlan V.M.
NeuroMolecular Med. 7:297-310(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AKAP8L, SUBCELLULAR LOCATION.
[7]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-928; SER-929; SER-931 AND SER-934, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-196, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF135439 mRNA. Translation: AAD39463.1.
AK041205 mRNA. Translation: BAC30863.1.
AK051375 mRNA. Translation: BAC34617.1.
AK087963 mRNA. Translation: BAC40061.2.
U40747 mRNA. Translation: AAC52475.1.
PIRS64713.
RefSeqNP_061255.1. NM_018785.2.
UniGeneMm.257474.
Mm.392945.
Mm.393219.

3D structure databases

ProteinModelPortalQ9R1C7.
SMRQ9R1C7. Positions 133-222, 378-446, 448-589, 606-661, 739-802.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207833. 5 interactions.
IntActQ9R1C7. 4 interactions.
MINTMINT-1525232.
STRING10090.ENSMUSP00000075655.

PTM databases

PhosphoSiteQ9R1C7.

Proteomic databases

PaxDbQ9R1C7.
PRIDEQ9R1C7.

Protocols and materials databases

DNASU56194.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000076313; ENSMUSP00000075655; ENSMUSG00000061136. [Q9R1C7-1]
GeneID56194.
KEGGmmu:56194.
UCSCuc008jrk.2. mouse. [Q9R1C7-1]

Organism-specific databases

CTD55660.
MGIMGI:1860512. Prpf40a.

Phylogenomic databases

eggNOGCOG5104.
GeneTreeENSGT00740000115182.
HOGENOMHOG000231802.
HOVERGENHBG059634.
InParanoidQ9R1C7.
KOK12821.
OMAMMGHPGM.
OrthoDBEOG77Q4X2.
PhylomeDBQ9R1C7.
TreeFamTF318732.

Gene expression databases

ArrayExpressQ9R1C7.
BgeeQ9R1C7.
GenevestigatorQ9R1C7.

Family and domain databases

Gene3D1.10.10.440. 4 hits.
InterProIPR002713. FF_domain.
IPR001202. WW_dom.
[Graphical view]
PfamPF01846. FF. 5 hits.
PF00397. WW. 2 hits.
[Graphical view]
SMARTSM00441. FF. 5 hits.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMSSF51045. SSF51045. 2 hits.
SSF81698. SSF81698. 5 hits.
PROSITEPS51676. FF. 6 hits.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio312006.
PROQ9R1C7.
SOURCESearch...

Entry information

Entry namePR40A_MOUSE
AccessionPrimary (citable) accession number: Q9R1C7
Secondary accession number(s): Q61049 expand/collapse secondary AC list , Q8BQ76, Q8BRW4, Q8C2U1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot