Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diacylglycerol kinase epsilon

Gene

Dgke

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri57 – 10650Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri121 – 17454Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107. 3474.
ReactomeiREACT_348749. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase epsilon (EC:2.7.1.107)
Short name:
DAG kinase epsilon
Alternative name(s):
Diglyceride kinase epsilon
Short name:
DGK-epsilon
Gene namesi
Name:Dgke
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1889276. Dgke.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4021HelicalSequence AnalysisAdd
BLAST
Transmembranei433 – 45321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Diacylglycerol kinase epsilonPRO_0000218465Add
BLAST

Proteomic databases

MaxQBiQ9R1C6.
PaxDbiQ9R1C6.
PRIDEiQ9R1C6.

PTM databases

PhosphoSiteiQ9R1C6.

Expressioni

Tissue specificityi

Expressed in platelets.1 Publication

Gene expression databases

BgeeiQ9R1C6.
CleanExiMM_DGKE.
ExpressionAtlasiQ9R1C6. baseline and differential.
GenevisibleiQ9R1C6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000285.

Structurei

3D structure databases

ProteinModelPortaliQ9R1C6.
SMRiQ9R1C6. Positions 114-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 353142DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri57 – 10650Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri121 – 17454Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000220913.
HOVERGENiHBG051347.
InParanoidiQ9R1C6.
KOiK00901.
OMAiTMKLKGQ.
OrthoDBiEOG71VSS6.
PhylomeDBiQ9R1C6.
TreeFamiTF313104.

Family and domain databases

InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R1C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGDQRSGPP AQSLLPDGHL VLWTLCSVLL PVFITLWCSL QRSRRQLHRR
60 70 80 90 100
DIFRKSKHCW RDTDLFSHPT YCCVCAQHIL QGAFCDCCGL RVDEGCLKKV
110 120 130 140 150
DKRFPCKEIM LKNDKAADAM PHHWIRGNVP LCSYCVFCRQ QCGSQPKLCD
160 170 180 190 200
YRCIWCQKTV HDECMRGSLR SEKCDFGEFR NLIIPPSYLT SINQMRKDKN
210 220 230 240 250
TNYEGLASKF GKQWTPLIIL ANSRSGTNMG EGLLGEFKIL LNPVQVFDVT
260 270 280 290 300
KTPPIKALQL CTLLPYYSVR VLVCGGDGTV GWVLDAIDEM KIKGQEKYIP
310 320 330 340 350
EVAVLPLGTG NDLSNTLGWG TGYAGEIPVA QVLRNVMEAD GIKLDRWKVQ
360 370 380 390 400
VTNKGYYNLR KPKEFTMNNY FSVGPDALMA LNFHAHREKA PSLFSSRILN
410 420 430 440 450
KAVYLFYGTK DCLVQECKDL NKKIELELDG ERVELPNLEG IIVLNIGYWG
460 470 480 490 500
GGCRLWEGMG DETYPLARHD DGLLEIVGVY GSFHCAQIQV KLANPFRIGQ
510 520 530 540 550
AHTVRLTLKC SMMPMQVDGE PWAQGPCTVT ITHKTHALML YFSGEQSDDD
560
ISSPSDHEDV KEAE
Length:564
Mass (Da):63,635
Last modified:May 1, 2000 - v1
Checksum:iC900BEC2CE9DD109
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136744 mRNA. Translation: AAD45665.1.
AL646096 Genomic DNA. Translation: CAI24267.1.
CCDSiCCDS25234.1.
RefSeqiNP_062378.1. NM_019505.3.
UniGeneiMm.153695.

Genome annotation databases

EnsembliENSMUST00000000285; ENSMUSP00000000285; ENSMUSG00000000276.
ENSMUST00000107894; ENSMUSP00000103526; ENSMUSG00000000276.
GeneIDi56077.
KEGGimmu:56077.
UCSCiuc007kwd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136744 mRNA. Translation: AAD45665.1.
AL646096 Genomic DNA. Translation: CAI24267.1.
CCDSiCCDS25234.1.
RefSeqiNP_062378.1. NM_019505.3.
UniGeneiMm.153695.

3D structure databases

ProteinModelPortaliQ9R1C6.
SMRiQ9R1C6. Positions 114-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000285.

PTM databases

PhosphoSiteiQ9R1C6.

Proteomic databases

MaxQBiQ9R1C6.
PaxDbiQ9R1C6.
PRIDEiQ9R1C6.

Protocols and materials databases

DNASUi56077.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000285; ENSMUSP00000000285; ENSMUSG00000000276.
ENSMUST00000107894; ENSMUSP00000103526; ENSMUSG00000000276.
GeneIDi56077.
KEGGimmu:56077.
UCSCiuc007kwd.1. mouse.

Organism-specific databases

CTDi8526.
MGIiMGI:1889276. Dgke.

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000220913.
HOVERGENiHBG051347.
InParanoidiQ9R1C6.
KOiK00901.
OMAiTMKLKGQ.
OrthoDBiEOG71VSS6.
PhylomeDBiQ9R1C6.
TreeFamiTF313104.

Enzyme and pathway databases

BRENDAi2.7.1.107. 3474.
ReactomeiREACT_348749. Effects of PIP2 hydrolysis.

Miscellaneous databases

NextBioi311868.
PROiQ9R1C6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R1C6.
CleanExiMM_DGKE.
ExpressionAtlasiQ9R1C6. baseline and differential.
GenevisibleiQ9R1C6. MM.

Family and domain databases

InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Diacylglycerol kinase epsilon regulates seizure susceptibility and long-term potentiation through arachidonoyl-inositol lipid signaling."
    Rodriguez de Turco E.B., Tang W., Topham M.K., Sakane F., Marcheselli V.L., Chen C., Taketomi A., Prescott S.M., Bazan N.G.
    Proc. Natl. Acad. Sci. U.S.A. 98:4740-4745(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiDGKE_MOUSE
AccessioniPrimary (citable) accession number: Q9R1C6
Secondary accession number(s): Q5SU69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: May 1, 2000
Last modified: July 22, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.