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Q9R1C6

- DGKE_MOUSE

UniProt

Q9R1C6 - DGKE_MOUSE

Protein

Diacylglycerol kinase epsilon

Gene

Dgke

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri57 – 10650Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri121 – 17454Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase epsilon (EC:2.7.1.107)
    Short name:
    DAG kinase epsilon
    Alternative name(s):
    Diglyceride kinase epsilon
    Short name:
    DGK-epsilon
    Gene namesi
    Name:Dgke
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1889276. Dgke.

    Subcellular locationi

    Membrane Curated; Multi-pass membrane protein Curated. Cytoplasm By similarity. Membrane By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 564564Diacylglycerol kinase epsilonPRO_0000218465Add
    BLAST

    Proteomic databases

    MaxQBiQ9R1C6.
    PaxDbiQ9R1C6.
    PRIDEiQ9R1C6.

    PTM databases

    PhosphoSiteiQ9R1C6.

    Expressioni

    Tissue specificityi

    Expressed in platelets.1 Publication

    Gene expression databases

    ArrayExpressiQ9R1C6.
    BgeeiQ9R1C6.
    CleanExiMM_DGKE.
    GenevestigatoriQ9R1C6.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000000285.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R1C6.
    SMRiQ9R1C6. Positions 114-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei20 – 4021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei433 – 45321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini212 – 353142DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri57 – 10650Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri121 – 17454Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00740000115137.
    HOGENOMiHOG000220913.
    HOVERGENiHBG051347.
    InParanoidiQ5SU69.
    KOiK00901.
    OMAiCMKTSLK.
    OrthoDBiEOG71VSS6.
    PhylomeDBiQ9R1C6.
    TreeFamiTF313104.

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R1C6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGDQRSGPP AQSLLPDGHL VLWTLCSVLL PVFITLWCSL QRSRRQLHRR    50
    DIFRKSKHCW RDTDLFSHPT YCCVCAQHIL QGAFCDCCGL RVDEGCLKKV 100
    DKRFPCKEIM LKNDKAADAM PHHWIRGNVP LCSYCVFCRQ QCGSQPKLCD 150
    YRCIWCQKTV HDECMRGSLR SEKCDFGEFR NLIIPPSYLT SINQMRKDKN 200
    TNYEGLASKF GKQWTPLIIL ANSRSGTNMG EGLLGEFKIL LNPVQVFDVT 250
    KTPPIKALQL CTLLPYYSVR VLVCGGDGTV GWVLDAIDEM KIKGQEKYIP 300
    EVAVLPLGTG NDLSNTLGWG TGYAGEIPVA QVLRNVMEAD GIKLDRWKVQ 350
    VTNKGYYNLR KPKEFTMNNY FSVGPDALMA LNFHAHREKA PSLFSSRILN 400
    KAVYLFYGTK DCLVQECKDL NKKIELELDG ERVELPNLEG IIVLNIGYWG 450
    GGCRLWEGMG DETYPLARHD DGLLEIVGVY GSFHCAQIQV KLANPFRIGQ 500
    AHTVRLTLKC SMMPMQVDGE PWAQGPCTVT ITHKTHALML YFSGEQSDDD 550
    ISSPSDHEDV KEAE 564
    Length:564
    Mass (Da):63,635
    Last modified:May 1, 2000 - v1
    Checksum:iC900BEC2CE9DD109
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF136744 mRNA. Translation: AAD45665.1.
    AL646096 Genomic DNA. Translation: CAI24267.1.
    CCDSiCCDS25234.1.
    RefSeqiNP_062378.1. NM_019505.3.
    UniGeneiMm.153695.

    Genome annotation databases

    EnsembliENSMUST00000000285; ENSMUSP00000000285; ENSMUSG00000000276.
    ENSMUST00000107894; ENSMUSP00000103526; ENSMUSG00000000276.
    GeneIDi56077.
    KEGGimmu:56077.
    UCSCiuc007kwd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF136744 mRNA. Translation: AAD45665.1 .
    AL646096 Genomic DNA. Translation: CAI24267.1 .
    CCDSi CCDS25234.1.
    RefSeqi NP_062378.1. NM_019505.3.
    UniGenei Mm.153695.

    3D structure databases

    ProteinModelPortali Q9R1C6.
    SMRi Q9R1C6. Positions 114-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000000285.

    PTM databases

    PhosphoSitei Q9R1C6.

    Proteomic databases

    MaxQBi Q9R1C6.
    PaxDbi Q9R1C6.
    PRIDEi Q9R1C6.

    Protocols and materials databases

    DNASUi 56077.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000285 ; ENSMUSP00000000285 ; ENSMUSG00000000276 .
    ENSMUST00000107894 ; ENSMUSP00000103526 ; ENSMUSG00000000276 .
    GeneIDi 56077.
    KEGGi mmu:56077.
    UCSCi uc007kwd.1. mouse.

    Organism-specific databases

    CTDi 8526.
    MGIi MGI:1889276. Dgke.

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00740000115137.
    HOGENOMi HOG000220913.
    HOVERGENi HBG051347.
    InParanoidi Q5SU69.
    KOi K00901.
    OMAi CMKTSLK.
    OrthoDBi EOG71VSS6.
    PhylomeDBi Q9R1C6.
    TreeFami TF313104.

    Enzyme and pathway databases

    Reactomei REACT_219232. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    NextBioi 311868.
    PROi Q9R1C6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R1C6.
    Bgeei Q9R1C6.
    CleanExi MM_DGKE.
    Genevestigatori Q9R1C6.

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Diacylglycerol kinase epsilon regulates seizure susceptibility and long-term potentiation through arachidonoyl-inositol lipid signaling."
      Rodriguez de Turco E.B., Tang W., Topham M.K., Sakane F., Marcheselli V.L., Chen C., Taketomi A., Prescott S.M., Bazan N.G.
      Proc. Natl. Acad. Sci. U.S.A. 98:4740-4745(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiDGKE_MOUSE
    AccessioniPrimary (citable) accession number: Q9R1C6
    Secondary accession number(s): Q5SU69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3