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Protein

Protein unc-13 homolog D

Gene

Unc13d

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca2+-dependent secretory lysosome exocytosis in mast cells (By similarity).By similarity

GO - Biological processi

  • exocytosis Source: UniProtKB-KW
  • secretion Source: RGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein unc-13 homolog D
Alternative name(s):
Munc13-4
Gene namesi
Name:Unc13d
Synonyms:Unc13h4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628593. Unc13d.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001885821 – 1088Protein unc-13 homolog DAdd BLAST1088

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei150PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9R189.
PRIDEiQ9R189.

PTM databases

iPTMnetiQ9R189.
PhosphoSitePlusiQ9R189.

Expressioni

Tissue specificityi

Expressed in lung bronchial epithelium goblet/mucous cells. Also expressed in spleen and testis. Expressed at very low levels in heart muscle, kidney, liver, brain and skeletal muscle.1 Publication

Interactioni

Subunit structurei

Interacts with RAB27A and DOC2A.By similarity

Protein-protein interaction databases

MINTiMINT-4566976.
STRINGi10116.ENSRNOP00000010466.

Structurei

3D structure databases

ProteinModelPortaliQ9R189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 221C2 1PROSITE-ProRule annotationAdd BLAST124
Domaini557 – 675MHD1PROSITE-ProRule annotationAdd BLAST119
Domaini786 – 893MHD2PROSITE-ProRule annotationAdd BLAST108
Domaini910 – 1017C2 2PROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni240 – 543Interaction with RAB27ABy similarityAdd BLAST304

Domaini

The MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome.By similarity

Sequence similaritiesi

Belongs to the unc-13 family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 MHD1 (MUNC13 homology domain 1) domain.PROSITE-ProRule annotation
Contains 1 MHD2 (MUNC13 homology domain 2) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1328. Eukaryota.
ENOG410Z3Q9. LUCA.
HOGENOMiHOG000060231.
HOVERGENiHBG104909.
InParanoidiQ9R189.
KOiK19728.
PhylomeDBiQ9R189.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF10540. Membr_traf_MHD. 2 hits.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 4 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHLSHPQR RPPLLRQAVK IRRRRVRDLQ DPPPQATPEV QVQSHHFSPE
60 70 80 90 100
ERDLLYEEAL YTVLHRLGQP EPNHVKEASE LLCYLQEAFQ VQPEEHQQML
110 120 130 140 150
QRVRELEKPI FCLKATVKQA KGILGKDVSG FSDPYCLLGI EQKVGVPEGS
160 170 180 190 200
PVSRRRQKAV VRHTIPEEET HRTQVKTQTL NPVWDETFIL EFEDITNASF
210 220 230 240 250
HLDMWDLDTV ESVRHKLGEL TDLHGLRRIF KEARKDKGQD DFLGNVMLRL
260 270 280 290 300
QDLRCREDQW FPLEPCTETY PDRGQCHLQF QFIHKRRATV ASRSQPSYTV
310 320 330 340 350
HFHLLQQLVS HEVTQHQAGG TSWGRIAESQ AVTILFLHAT QKDLSDFHQS
360 370 380 390 400
MAQWLAYSRL YQSLEFPSSC LLHPITSIEY QWIQGRLKAE QREELATSFT
410 420 430 440 450
SLLAYGLSLI RKFRSVFPLS VSDSPSRLQS LLRVLVQMCK MKAFGELCPD
460 470 480 490 500
SAPLPQLVSE ALRMGTVEWF HLMQQHHQPM VQGILEAGKA LLSLVQDVMG
510 520 530 540 550
DLYQCRRTWN KIFHNVLKID LFTMAFLELQ WLVAKRVQDH TAAVGDLVSP
560 570 580 590 600
DVGESLFQLY VSLREFCQLG PSDSHEVLAL DGFHRWFQSA IPSWLQRTYS
610 620 630 640 650
VALERVQRAV QMDSLVPLGE LTKHSTSAVD LSTCFAQISH TARQLDWPDP
660 670 680 690 700
EEAFMITVKF VEDTCRLALV YCSLIKARAR ELSAVQKDQS QAADMLCVVV
710 720 730 740 750
NNVERLRLII DKLPTQLAWE ALEQRVGAVL EQGQLQNTLH TQLQGALAGL
760 770 780 790 800
GHEIRTGVRT LAEQLEVGIA THIQKLIDAK GSILPEDAIL PLMKFLEVKL
810 820 830 840 850
CYMNTNLVQE NFSSLLTLLW THTLTVLVEA AASHRNSSLA SSRLKVALQN
860 870 880 890 900
LEICFHAEGC GLPPEALHTD TFLALQSDLE LQAASSRELI QKYFRSRIQQ
910 920 930 940 950
QAETTSERLG AVTVKASYRA SEQKLHVELL SASSLLPLDS NGSSDPFVQL
960 970 980 990 1000
TLEPRHEFPE LAPRETQKHK KELHPLFDET FEFLVPAEPC QKDGACLLLT
1010 1020 1030 1040 1050
VLDHDRLGAD DLEGEAFLPL CRVPGLTGCV EPGEAPQMRL PLTYPAPNGD
1060 1070 1080
PILRLLESRK GDREAQAFVK LRRQRAKQAS QHAPATEP
Length:1,088
Mass (Da):123,454
Last modified:May 1, 2000 - v1
Checksum:i1646C55EEC22541C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159356 mRNA. Translation: AAD44333.1.
RefSeqiNP_620199.1. NM_138844.1.
UniGeneiRn.198919.

Genome annotation databases

GeneIDi192177.
KEGGirno:192177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF159356 mRNA. Translation: AAD44333.1.
RefSeqiNP_620199.1. NM_138844.1.
UniGeneiRn.198919.

3D structure databases

ProteinModelPortaliQ9R189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4566976.
STRINGi10116.ENSRNOP00000010466.

PTM databases

iPTMnetiQ9R189.
PhosphoSitePlusiQ9R189.

Proteomic databases

PaxDbiQ9R189.
PRIDEiQ9R189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192177.
KEGGirno:192177.

Organism-specific databases

CTDi201294.
RGDi628593. Unc13d.

Phylogenomic databases

eggNOGiKOG1328. Eukaryota.
ENOG410Z3Q9. LUCA.
HOGENOMiHOG000060231.
HOVERGENiHBG104909.
InParanoidiQ9R189.
KOiK19728.
PhylomeDBiQ9R189.

Miscellaneous databases

PROiQ9R189.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF10540. Membr_traf_MHD. 2 hits.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 4 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUN13D_RAT
AccessioniPrimary (citable) accession number: Q9R189
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.