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Q9R186 (TRPV6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Transient receptor potential cation channel subfamily V member 6
Short name=TrpV6
Alternative name(s):
Calcium transport protein 1
Short name=CaT1
Epithelial calcium channel 2
Short name=ECaC2
Gene names
Name:Trpv6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium selective cation channel probably involved in Ca2+ uptake in various tissues, including Ca2+ reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca2+-dependent and a slower Ca2+-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg2+ in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating By similarity. Ref.2

Subunit structure

Homotetramer and probably heterotetramer with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in duodenum, proximal jejunum, cecum, and colon. Ref.1

Post-translational modification

Glycosylated By similarity.

Phosphorylation at Tyr-161 and Tyr-162 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at these sites by PTPN1. Ref.3

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily. [View classification]

Contains 6 ANK repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 727727Transient receptor potential cation channel subfamily V member 6
PRO_0000215356

Regions

Topological domain1 – 326326Cytoplasmic Potential
Transmembrane327 – 34721Helical; Potential
Topological domain348 – 38639Extracellular Potential
Transmembrane387 – 40721Helical; Potential
Topological domain408 – 41811Cytoplasmic Potential
Transmembrane419 – 43921Helical; Potential
Topological domain440 – 4478Extracellular Potential
Transmembrane448 – 46821Helical; Potential
Topological domain469 – 49123Cytoplasmic Potential
Transmembrane492 – 51221Helical; Potential
Intramembrane523 – 54321Pore-forming; By similarity
Transmembrane556 – 57621Helical; Potential
Topological domain577 – 727151Cytoplasmic Potential
Repeat44 – 7431ANK 1
Repeat78 – 10730ANK 2
Repeat116 – 14530ANK 3
Repeat162 – 19130ANK 4
Repeat195 – 23642ANK 5
Repeat238 – 26730ANK 6
Region93 – 10311Interaction with calmodulin By similarity
Region597 – 6015Interaction with S100A10 By similarity
Region649 – 66719Interaction with calmodulin By similarity
Motif540 – 5445Selectivity filter By similarity

Amino acid modifications

Modified residue1611Phosphotyrosine; by SRC Ref.3
Modified residue1621Phosphotyrosine; by SRC Ref.3
Glycosylation3571N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis1611Y → F: Prevents up-regulation of the channel by phosphorylation; when associated with F-162. Ref.3
Mutagenesis1621Y → F: Prevents up-regulation of the channel by phosphorylation; when associated with F-161. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9R186 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 1E996843A628C535

FASTA72783,236
        10         20         30         40         50         60 
MGWSLPKEKG LILCLWNKFC RWFHRRESWA QSRDEQNLLQ QKRIWESPLL LAAKENNVQA 

        70         80         90        100        110        120 
LIKLLKFEGC EVHQKGAMGE TALHIAALYD NLEAAMVLME AAPELVFEPM TSELYEGQTA 

       130        140        150        160        170        180 
LHIAVINQNV NLVRALLARG ASVSARATGS VFHYRPHNLI YYGEHPLSFA ACVGSEEIVR 

       190        200        210        220        230        240 
LLIEHGADIR AQDSLGNTVL HILILQPNKT FACQMYNLLL SYDGGDHLKS LELVPNNQGL 

       250        260        270        280        290        300 
TPFKLAGVEG NIVMFQHLMQ KRKHIQWTYG PLTSTLYDLT EIDSSGDDQS LLELIVTTKK 

       310        320        330        340        350        360 
REARQILDQT PVKELVSLKW KRYGRPYFCV LGAIYVLYII CFTMCCVYRP LKPRITNRTN 

       370        380        390        400        410        420 
PRDNTLLQQK LLQEAYVTPK DDLRLVGELV SIVGAVIILL VEIPDIFRLG VTRFFGQTIL 

       430        440        450        460        470        480 
GGPFHVIIVT YAFMVLVTMV MRLTNSDGEV VPMSFALVLG WCNVMYFARG FQMLGPFTIM 

       490        500        510        520        530        540 
IQKMIFGDLM RFCWLMAVVI LGFASAFYII FQTEDPDELG HFYDYPMALF STFELFLTII 

       550        560        570        580        590        600 
DGPANYDVDL PFMYSITYAA FAIIATLLML NLLIAMMGDT HWRVAHERDE LWRAQVVATT 

       610        620        630        640        650        660 
VMLERKLPRC LWPRSGICGR EYGLGDRWFL RVEDRQDLNR QRIRRYAQAF QQQDDLYSED 

       670        680        690        700        710        720 
LEKDSGEKLE MARPFGAYLS FPTPSVSRST SRSSTNWDRL RQGALRKDLQ GIINRGLEDG 


EGWEYQI

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References

[1]"Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption."
Peng J.-B., Chen X.Z., Berger U.V., Vassilev P.M., Tsukaguchi H., Brown E.M., Hediger M.A.
J. Biol. Chem. 274:22739-22746(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Intestine.
[2]"CaT1 manifests the pore properties of the calcium-release-activated calcium channel."
Yue L., Peng J.B., Hediger M.A., Clapham D.E.
Nature 410:705-709(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"Identification of tyrosines in the putative regulatory site of the Ca2+ channel TRPV6."
Sternfeld L., Anderie I., Schmid A., Al-Shaldi H., Krause E., Magg T., Schreiner D., Hofer H.W., Schulz I.
Cell Calcium 42:91-102(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-161 AND TYR-162, MUTAGENESIS OF TYR-161 AND TYR-162.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF160798 mRNA. Translation: AAD47636.1.
IPIIPI00199537.
RefSeqNP_446138.1. NM_053686.1.
UniGeneRn.205943.

3D structure databases

ProteinModelPortalQ9R186.
SMRQ9R186. Positions 44-265.
ModBaseSearch...

Protein family/group databases

TCDB1.A.4.2.7. transient receptor potential Ca2+ channel (TRP-CC) family.

PTM databases

PhosphoSiteQ9R186.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID114246.
KEGGrno:114246.
UCSCRGD:69335. rat.

Organism-specific databases

CTD55503.
RGD69335. Trpv6.

Phylogenomic databases

eggNOGNOG278734.
HOGENOMHOG000234397.
HOVERGENHBG061442.
InParanoidQ9R186.
KOK04975.
OrthoDBEOG4SBDXG.

Gene expression databases

ArrayExpressQ9R186.
GenevestigatorQ9R186.
GermOnlineENSRNOG00000014714. Rattus norvegicus.

Family and domain databases

Gene3D1.25.40.20. 2 hits.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6_channel.
IPR008345. TRPV6_channel.
IPR024862. TRPV_channel.
[Graphical view]
PANTHERPTHR10582. PTHR10582. 1 hit.
PTHR10582:SF1. PTHR10582:SF1. 1 hit.
PfamPF12796. Ank_2. 2 hits.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01766. ECACCHANNEL1.
SMARTSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMSSF48403. ANK. 1 hit.
TIGRFAMsTIGR00870. trp. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio618459.

Entry information

Entry nameTRPV6_RAT
AccessionPrimary (citable) accession number: Q9R186
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 2000
Last modified: April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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