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Protein

Transient receptor potential cation channel subfamily V member 6

Gene

Trpv6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium selective cation channel probably involved in Ca2+ uptake in various tissues, including Ca2+ reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca2+-dependent and a slower Ca2+-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg2+ in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).By similarity1 Publication

GO - Molecular functioni

  • calcium activated cation channel activity Source: RGD
  • calcium channel activity Source: RGD

GO - Biological processi

  • calcium ion import Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding

Protein family/group databases

TCDBi1.A.4.2.7. the transient receptor potential ca(2+) channel (trp-cc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 6
Short name:
TrpV6
Alternative name(s):
Calcium transport protein 1
Short name:
CaT1
Epithelial calcium channel 2
Short name:
ECaC2
Gene namesi
Name:Trpv6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69335. Trpv6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 366366CytoplasmicSequence analysisAdd
BLAST
Transmembranei367 – 38721HelicalSequence analysisAdd
BLAST
Topological domaini388 – 42639ExtracellularSequence analysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence analysisAdd
BLAST
Topological domaini448 – 45811CytoplasmicSequence analysisAdd
BLAST
Transmembranei459 – 47921HelicalSequence analysisAdd
BLAST
Topological domaini480 – 4878ExtracellularSequence analysis
Transmembranei488 – 50821HelicalSequence analysisAdd
BLAST
Topological domaini509 – 53123CytoplasmicSequence analysisAdd
BLAST
Transmembranei532 – 55221HelicalSequence analysisAdd
BLAST
Intramembranei563 – 58321Pore-formingBy similarityAdd
BLAST
Transmembranei596 – 61621HelicalSequence analysisAdd
BLAST
Topological domaini617 – 767151CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi201 – 2011Y → F: Prevents up-regulation of the channel by phosphorylation; when associated with F-202. 1 Publication
Mutagenesisi202 – 2021Y → F: Prevents up-regulation of the channel by phosphorylation; when associated with F-201. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Transient receptor potential cation channel subfamily V member 6PRO_0000215356Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011Phosphotyrosine; by SRC1 Publication
Modified residuei202 – 2021Phosphotyrosine; by SRC1 Publication
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.By similarity
Phosphorylation at Tyr-201 and Tyr-202 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at these sites by PTPN1.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9R186.

PTM databases

iPTMnetiQ9R186.
PhosphoSiteiQ9R186.

Expressioni

Tissue specificityi

Expressed in duodenum, proximal jejunum, cecum, and colon.1 Publication

Interactioni

Subunit structurei

Homotetramer and probably heterotetramer with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY. Interacts with TCAF1 and TCAF2 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9R186. 1 interaction.
STRINGi10116.ENSRNOP00000020616.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati84 – 11431ANK 1Add
BLAST
Repeati118 – 14730ANK 2Add
BLAST
Repeati156 – 18530ANK 3Add
BLAST
Repeati202 – 23130ANK 4Add
BLAST
Repeati235 – 27642ANK 5Add
BLAST
Repeati278 – 30730ANK 6Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 14311Interaction with calmodulinBy similarityAdd
BLAST
Regioni637 – 6415Interaction with S100A10By similarity
Regioni689 – 70719Interaction with calmodulinBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi580 – 5845Selectivity filterBy similarity

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ9R186.
KOiK04975.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6.
IPR008345. TRPV6_channel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01766. ECACCHANNEL1.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPLQREGRP ALGDANVAPG SSPGGVWHQP QPPKDSAFHP MGWSLPKEKG
60 70 80 90 100
LILCLWNKFC RWFHRRESWA QSRDEQNLLQ QKRIWESPLL LAAKENNVQA
110 120 130 140 150
LIKLLKFEGC EVHQKGAMGE TALHIAALYD NLEAAMVLME AAPELVFEPM
160 170 180 190 200
TSELYEGQTA LHIAVINQNV NLVRALLARG ASVSARATGS VFHYRPHNLI
210 220 230 240 250
YYGEHPLSFA ACVGSEEIVR LLIEHGADIR AQDSLGNTVL HILILQPNKT
260 270 280 290 300
FACQMYNLLL SYDGGDHLKS LELVPNNQGL TPFKLAGVEG NIVMFQHLMQ
310 320 330 340 350
KRKHIQWTYG PLTSTLYDLT EIDSSGDDQS LLELIVTTKK REARQILDQT
360 370 380 390 400
PVKELVSLKW KRYGRPYFCV LGAIYVLYII CFTMCCVYRP LKPRITNRTN
410 420 430 440 450
PRDNTLLQQK LLQEAYVTPK DDLRLVGELV SIVGAVIILL VEIPDIFRLG
460 470 480 490 500
VTRFFGQTIL GGPFHVIIVT YAFMVLVTMV MRLTNSDGEV VPMSFALVLG
510 520 530 540 550
WCNVMYFARG FQMLGPFTIM IQKMIFGDLM RFCWLMAVVI LGFASAFYII
560 570 580 590 600
FQTEDPDELG HFYDYPMALF STFELFLTII DGPANYDVDL PFMYSITYAA
610 620 630 640 650
FAIIATLLML NLLIAMMGDT HWRVAHERDE LWRAQVVATT VMLERKLPRC
660 670 680 690 700
LWPRSGICGR EYGLGDRWFL RVEDRQDLNR QRIRRYAQAF QQQDDLYSED
710 720 730 740 750
LEKDSGEKLE MARPFGAYLS FPTPSVSRST SRSSTNWDRL RQGALRKDLQ
760
GIINRGLEDG EGWEYQI
Length:767
Mass (Da):87,362
Last modified:April 1, 2015 - v2
Checksum:i2197726C2F583720
GO

Sequence cautioni

The sequence AAD47636.1 differs from that shown.Unusual initiator. The initiator methionine is coded by a non-canonical ACG threonine codon.By similarity

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF160798 mRNA. Translation: AAD47636.1. Sequence problems.
RefSeqiNP_446138.1. NM_053686.1.
UniGeneiRn.205943.

Genome annotation databases

GeneIDi114246.
KEGGirno:114246.
UCSCiRGD:69335. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF160798 mRNA. Translation: AAD47636.1. Sequence problems.
RefSeqiNP_446138.1. NM_053686.1.
UniGeneiRn.205943.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R186. 1 interaction.
STRINGi10116.ENSRNOP00000020616.

Protein family/group databases

TCDBi1.A.4.2.7. the transient receptor potential ca(2+) channel (trp-cc) family.

PTM databases

iPTMnetiQ9R186.
PhosphoSiteiQ9R186.

Proteomic databases

PaxDbiQ9R186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114246.
KEGGirno:114246.
UCSCiRGD:69335. rat.

Organism-specific databases

CTDi55503.
RGDi69335. Trpv6.

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ9R186.
KOiK04975.

Miscellaneous databases

PROiQ9R186.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6.
IPR008345. TRPV6_channel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01766. ECACCHANNEL1.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption."
    Peng J.-B., Chen X.Z., Berger U.V., Vassilev P.M., Tsukaguchi H., Brown E.M., Hediger M.A.
    J. Biol. Chem. 274:22739-22746(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Intestine.
  2. "CaT1 manifests the pore properties of the calcium-release-activated calcium channel."
    Yue L., Peng J.B., Hediger M.A., Clapham D.E.
    Nature 410:705-709(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Identification of tyrosines in the putative regulatory site of the Ca2+ channel TRPV6."
    Sternfeld L., Anderie I., Schmid A., Al-Shaldi H., Krause E., Magg T., Schreiner D., Hofer H.W., Schulz I.
    Cell Calcium 42:91-102(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-201 AND TYR-202, MUTAGENESIS OF TYR-201 AND TYR-202.

Entry informationi

Entry nameiTRPV6_RAT
AccessioniPrimary (citable) accession number: Q9R186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 1, 2015
Last modified: June 8, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-41 is the initiator. In human and mouse, initiation starts at a conserved non-canonical ACG threonine codon decoded as Met-1 upstream of the canonical initiation at Met-41.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.