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Protein

Transient receptor potential cation channel subfamily V member 6

Gene

Trpv6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium selective cation channel that mediates Ca2+ uptake in various tissues, including the intestine (PubMed:10428857, PubMed:11287959, PubMed:27296226). Important for normal Ca2+ ion homeostasis in the body, including bone and skin (By similarity). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification (PubMed:10428857, PubMed:11287959, PubMed:27296226). Inactivation includes both a rapid Ca2+-dependent and a slower Ca2+-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg2+ in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi581Calcium; shared with neighboring subunits1 Publication1

GO - Molecular functioni

  • calcium activated cation channel activity Source: RGD
  • calcium channel activity Source: UniProtKB
  • calmodulin binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • calcium ion homeostasis Source: UniProtKB
  • calcium ion import Source: RGD
  • calcium ion import into cell Source: UniProtKB
  • calcium ion transmembrane transport Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
  • response to calcium ion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Calmodulin-binding, Metal-binding

Protein family/group databases

TCDBi1.A.4.2.7. the transient receptor potential ca(2+) channel (trp-cc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 6
Short name:
TrpV6
Alternative name(s):
Calcium transport protein 11 Publication
Short name:
CaT11 Publication
Epithelial calcium channel 2
Short name:
ECaC2
Gene namesi
Name:Trpv6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69335. Trpv6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 366Cytoplasmic1 PublicationAdd BLAST366
Transmembranei367 – 387Helical1 PublicationAdd BLAST21
Topological domaini388 – 424Extracellular1 PublicationAdd BLAST37
Transmembranei425 – 447Helical1 PublicationAdd BLAST23
Topological domaini448 – 462Cytoplasmic1 PublicationAdd BLAST15
Transmembranei463 – 482Helical1 PublicationAdd BLAST20
Topological domaini483 – 488Extracellular1 Publication6
Transmembranei489 – 508Helical1 PublicationAdd BLAST20
Topological domaini509 – 528CytoplasmicSequence analysisAdd BLAST20
Transmembranei529 – 551Helical1 PublicationAdd BLAST23
Intramembranei565 – 584Pore-forming1 PublicationAdd BLAST20
Transmembranei596 – 616Helical1 PublicationAdd BLAST21
Topological domaini617 – 767Cytoplasmic1 PublicationAdd BLAST151

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi201Y → F: Prevents up-regulation of the channel by phosphorylation; when associated with F-202. 1 Publication1
Mutagenesisi202Y → F: Prevents up-regulation of the channel by phosphorylation; when associated with F-201. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153561 – 767Transient receptor potential cation channel subfamily V member 6Add BLAST767

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201Phosphotyrosine; by SRC1 Publication1
Modified residuei202Phosphotyrosine; by SRC1 Publication1
Glycosylationi397N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.By similarity
Phosphorylation at Tyr-201 and Tyr-202 by SRC leads to an increased calcium influx through the channel. Probably dephosphorylated at these sites by PTPN1.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9R186.

PTM databases

iPTMnetiQ9R186.
PhosphoSitePlusiQ9R186.

Expressioni

Tissue specificityi

Expressed in duodenum, proximal jejunum, cecum, and colon.1 Publication

Interactioni

Subunit structurei

Homotetramer (PubMed:27296226). Probably forms also heterotetramers with TRPV5. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY. Interacts with TCAF1 and TCAF2 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-47769N.
IntActiQ9R186. 1 interactor.
STRINGi10116.ENSRNOP00000020616.

Structurei

Secondary structure

1767
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi69 – 86Combined sources18
Helixi88 – 95Combined sources8
Helixi98 – 105Combined sources8
Helixi122 – 128Combined sources7
Helixi132 – 141Combined sources10
Helixi145 – 147Combined sources3
Turni154 – 157Combined sources4
Helixi160 – 166Combined sources7
Helixi170 – 178Combined sources9
Helixi206 – 213Combined sources8
Helixi216 – 224Combined sources9
Helixi239 – 244Combined sources6
Helixi249 – 261Combined sources13
Beta strandi266 – 269Combined sources4
Turni271 – 273Combined sources3
Helixi282 – 288Combined sources7
Helixi292 – 300Combined sources9
Beta strandi303 – 309Combined sources7
Beta strandi312 – 317Combined sources6
Beta strandi326 – 328Combined sources3
Helixi331 – 337Combined sources7
Helixi341 – 346Combined sources6
Turni350 – 352Combined sources3
Helixi353 – 361Combined sources9
Helixi364 – 387Combined sources24
Helixi397 – 399Combined sources3
Beta strandi400 – 402Combined sources3
Beta strandi419 – 421Combined sources3
Helixi422 – 446Combined sources25
Helixi464 – 481Combined sources18
Turni482 – 485Combined sources4
Helixi487 – 489Combined sources3
Helixi490 – 502Combined sources13
Turni503 – 506Combined sources4
Helixi507 – 509Combined sources3
Helixi521 – 552Combined sources32
Beta strandi556 – 559Combined sources4
Helixi565 – 576Combined sources12
Beta strandi586 – 589Combined sources4
Helixi594 – 617Combined sources24
Turni618 – 620Combined sources3
Helixi625 – 646Combined sources22
Beta strandi655 – 658Combined sources4
Turni660 – 663Combined sources4
Beta strandi667 – 675Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IWKX-ray3.25A41-709[»]
5IWPX-ray3.65A41-709[»]
5IWRX-ray3.85A41-709[»]
5IWTX-ray3.80A41-709[»]
SMRiQ9R186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati84 – 114ANK 1Sequence analysisAdd BLAST31
Repeati118 – 147ANK 2Sequence analysisAdd BLAST30
Repeati156 – 185ANK 3Sequence analysisAdd BLAST30
Repeati202 – 231ANK 4Sequence analysisAdd BLAST30
Repeati235 – 276ANK 5Sequence analysisAdd BLAST42
Repeati278 – 307ANK 6Sequence analysisAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 143Interaction with calmodulinBy similarityAdd BLAST11
Regioni637 – 641Interaction with S100A10By similarity5
Regioni689 – 707Interaction with calmodulinBy similarityAdd BLAST19

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi580 – 584Selectivity filter1 Publication5

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ9R186.
KOiK04975.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6.
IPR008345. TRPV6_channel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01766. ECACCHANNEL1.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPLQREGRP ALGDANVAPG SSPGGVWHQP QPPKDSAFHP MGWSLPKEKG
60 70 80 90 100
LILCLWNKFC RWFHRRESWA QSRDEQNLLQ QKRIWESPLL LAAKENNVQA
110 120 130 140 150
LIKLLKFEGC EVHQKGAMGE TALHIAALYD NLEAAMVLME AAPELVFEPM
160 170 180 190 200
TSELYEGQTA LHIAVINQNV NLVRALLARG ASVSARATGS VFHYRPHNLI
210 220 230 240 250
YYGEHPLSFA ACVGSEEIVR LLIEHGADIR AQDSLGNTVL HILILQPNKT
260 270 280 290 300
FACQMYNLLL SYDGGDHLKS LELVPNNQGL TPFKLAGVEG NIVMFQHLMQ
310 320 330 340 350
KRKHIQWTYG PLTSTLYDLT EIDSSGDDQS LLELIVTTKK REARQILDQT
360 370 380 390 400
PVKELVSLKW KRYGRPYFCV LGAIYVLYII CFTMCCVYRP LKPRITNRTN
410 420 430 440 450
PRDNTLLQQK LLQEAYVTPK DDLRLVGELV SIVGAVIILL VEIPDIFRLG
460 470 480 490 500
VTRFFGQTIL GGPFHVIIVT YAFMVLVTMV MRLTNSDGEV VPMSFALVLG
510 520 530 540 550
WCNVMYFARG FQMLGPFTIM IQKMIFGDLM RFCWLMAVVI LGFASAFYII
560 570 580 590 600
FQTEDPDELG HFYDYPMALF STFELFLTII DGPANYDVDL PFMYSITYAA
610 620 630 640 650
FAIIATLLML NLLIAMMGDT HWRVAHERDE LWRAQVVATT VMLERKLPRC
660 670 680 690 700
LWPRSGICGR EYGLGDRWFL RVEDRQDLNR QRIRRYAQAF QQQDDLYSED
710 720 730 740 750
LEKDSGEKLE MARPFGAYLS FPTPSVSRST SRSSTNWDRL RQGALRKDLQ
760
GIINRGLEDG EGWEYQI
Length:767
Mass (Da):87,362
Last modified:April 1, 2015 - v2
Checksum:i2197726C2F583720
GO

Sequence cautioni

The sequence AAD47636 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical ACG threonine codon.By similarity

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF160798 mRNA. Translation: AAD47636.1. Sequence problems.
RefSeqiNP_446138.1. NM_053686.1.
UniGeneiRn.205943.

Genome annotation databases

GeneIDi114246.
KEGGirno:114246.
UCSCiRGD:69335. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF160798 mRNA. Translation: AAD47636.1. Sequence problems.
RefSeqiNP_446138.1. NM_053686.1.
UniGeneiRn.205943.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IWKX-ray3.25A41-709[»]
5IWPX-ray3.65A41-709[»]
5IWRX-ray3.85A41-709[»]
5IWTX-ray3.80A41-709[»]
SMRiQ9R186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47769N.
IntActiQ9R186. 1 interactor.
STRINGi10116.ENSRNOP00000020616.

Protein family/group databases

TCDBi1.A.4.2.7. the transient receptor potential ca(2+) channel (trp-cc) family.

PTM databases

iPTMnetiQ9R186.
PhosphoSitePlusiQ9R186.

Proteomic databases

PaxDbiQ9R186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114246.
KEGGirno:114246.
UCSCiRGD:69335. rat.

Organism-specific databases

CTDi55503.
RGDi69335. Trpv6.

Phylogenomic databases

eggNOGiKOG3676. Eukaryota.
ENOG4110DG4. LUCA.
HOGENOMiHOG000234397.
HOVERGENiHBG061442.
InParanoidiQ9R186.
KOiK04975.

Miscellaneous databases

PROiQ9R186.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008344. TRPV5/TRPV6.
IPR008345. TRPV6_channel.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01765. ECACCHANNEL.
PR01766. ECACCHANNEL1.
SMARTiSM00248. ANK. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV6_RAT
AccessioniPrimary (citable) accession number: Q9R186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 1, 2015
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-41 is the initiator. In human and mouse, initiation starts at a conserved non-canonical ACG threonine codon decoded as Met-1 upstream of the canonical initiation at Met-41.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.