Reviewed, UniProtKB/Swiss-Prot Q9R168 (SFRP1_RAT)

Names and origin

Protein names

Recommended name:
    Secreted frizzled-related protein 1
      Short name=sFRP-1

Gene names

Name:

Sfrp1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	158 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels By similarity. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
Subunit structure	Interacts with WNT4. Interects with WNT1, WNT2, WNT8 and FRZD6 By similarity.
Subcellular location	Secreted By similarity. Note: Cell membrane or extracellular matrix-associated. Released by heparin-binding By similarity.
Developmental stage	Differentially expressed in developing kidney. In 16 dpc embryos, highly expressed around the ureter and, also in all stromal elements, including the interstitial populations of the medulla and the outer layer of the cortex. In 19 dpc embryos, expression in the medulla is increased and is also found in the mesenchymal tissue surrounding the developing ureter. Ref.1
Domain	The FZ domain is involved in binding with Wnt ligands By similarity.
Sequence similarities	Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain.

Ontologies

Keywords
Biological process	Differentiation Wnt signaling pathway
Cellular component	Secreted
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of Wnt receptor signaling pathway Traceable author statement. Source: RGD regulation of ossification Inferred from mutant phenotype. Source: RGD
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›158	›158	Secreted frizzled-related protein 1		PRO_0000191647
Regions
	Domain	‹1 – 34	›34	FZ
	Domain	51 – ›158	›108	NTR
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	51 ↔ 121		By similarity
	Disulfide bond	68 ↔ 123		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	158	1

Sequences

	Sequence		Length	Mass (Da)
	Q9R168-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: ABB1F13E3FB62690	FASTA	158	18,072
	10 20 30 40 50 60 VRDSCEPVMQ FFGFYWPEML KCDKFPEGDV CIAMTPPNAT EASKPQGTTV CPPCDNELKS 70 80 90 100 110 120 EAIIEHLCAS EFALRMKIKE VKKENGDKKI VPKKKKPLKL GPIKKKELKR LVLFLKNGAD 130 140 150 CPCHQLDNLS HNFLIMGRKV KSQYLLTAIH KWDKKNKE

References

[1]	"Secreted Frizzled-related proteins can regulate metanephric development." Yoshino K., Rubin J.S., Higinbotham K.G., Uren A., Anest V., Plisov S.Y., Perantoni A.O. Mech. Dev. 102:45-55(2001) [PubMed: 11287180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: Fischer.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF167308 mRNA. Translation: AAD49337.1.
IPI	IPI00188165.
UniGene	Rn.163333
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9R168.
Genome annotation databases
Ensembl	ENSRNOT00000024128; ENSRNOP00000024128; ENSRNOG00000017783; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	621074. Sfrp1.
Phylogenomic databases
HOVERGEN	Q9R168.
Gene expression databases
ArrayExpress	Q9R168.
Genevestigator	Q9R168.
GermOnline	ENSRNOG00000017783. Rattus norvegicus.
Family and domain databases
InterPro	IPR020067. Frizzled-like_dom. IPR000024. Frizzled_Cys-rich. IPR015526. Frizzled_related. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. [Graphical view]
Gene3D	G3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHER	PTHR11309. Fz_related. 1 hit.
Pfam	PF01392. Fz. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
SMART	SM00643. C345C. 1 hit. [Graphical view]
PROSITE	PS50038. FZ. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SFRP1_RAT

Accession

Primary (citable) accession number: Q9R168

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	May 1, 2000
Last modified:	October 13, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)