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Protein

Disintegrin and metalloproteinase domain-containing protein 18

Gene

Adam18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein.

GO - Molecular functioni

  • integrin binding Source: MGI
  • metalloendopeptidase activity Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Protein family/group databases

MEROPSiM12.958.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 18
Short name:
ADAM 18
Alternative name(s):
Disintegrin and metalloproteinase domain-containing protein 27
Short name:
ADAM 27
Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
Short name:
tMDC III
Gene namesi
Name:Adam18
Synonyms:Adam27, Dtgn3, Tmdc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:105986. Adam18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini173 – 683511ExtracellularSequence AnalysisAdd
BLAST
Transmembranei684 – 70421HelicalSequence AnalysisAdd
BLAST
Topological domaini705 – 71915CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Propeptidei20 – 179160Sequence AnalysisPRO_0000029100Add
BLAST
Chaini180 – 719540Disintegrin and metalloproteinase domain-containing protein 18PRO_0000029101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi289 ↔ 373By similarity
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi332 ↔ 357By similarity
Disulfide bondi334 ↔ 339By similarity
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi447 ↔ 468By similarity
Glycosylationi607 – 6071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi614 – 6141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi620 ↔ 632By similarity
Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi626 ↔ 638By similarity
Disulfide bondi640 ↔ 649By similarity

Post-translational modificationi

The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9R157.
PRIDEiQ9R157.

PTM databases

PhosphoSiteiQ9R157.

Expressioni

Tissue specificityi

Expressed specifically in testis.

Developmental stagei

Adult levels are reached by day 16 after birth.

Gene expression databases

BgeeiQ9R157.
CleanExiMM_ADAM18.
ExpressionAtlasiQ9R157. baseline and differential.
GenevisibleiQ9R157. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033957.

Structurei

3D structure databases

ProteinModelPortaliQ9R157.
SMRiQ9R157. Positions 182-650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini180 – 378199Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini387 – 47690DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini616 – 65035EGF-likePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi477 – 615139Cys-richAdd
BLAST

Domaini

A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG329803.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ9R157.
KOiK16909.
OMAiDYMGSEM.
OrthoDBiEOG7W1537.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR000742. EG-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLFILAEL AMLFARLDSE GICLHITVPQ KIEPRKGGDA EGKVTYVITI
60 70 80 90 100
DGKPYSLHLR NHSFLSQNFL VYTYNETGSL YSDSSHFLAH CHYRGYVDEV
110 120 130 140 150
PNSIVTLSIC SGLRGFLQLE NVSYGIEPLE SSARFEHIVY QVKSDSSMLA
160 170 180 190 200
GNDSHVWQID QLDKGHFNEQ DKNHSQLLPQ SLKLHIIVGK FLFDYMGSDI
210 220 230 240 250
MAITQKIFQI IGLVNAMLTQ LKLSVVLASL ELWSDKNHIS TDGNATDILQ
260 270 280 290 300
RLLDWKRDYL TLQSNEITHL LIYRRRPKYI GAASPGEICS KSYVAGVGMY
310 320 330 340 350
PEDIGLEGFS VVITQLIGLH IGLTYDDNIR NCSCPSAPCI MQQGALSSSG
360 370 380 390 400
KKTFSNCSLH DYMHYVSNFD TQCLGDLSNV HVLQPNQAVC GNGIMEAGEE
410 420 430 440 450
CDCGNETECQ FKECCDHETC RLKGSAQCGS GACCMPTCEL SASGTPCRKA
460 470 480 490 500
VDPECDFTEY CDGSSSHCVP DTFALNGHLC RLGSAYCYNG RCQALNDQCV
510 520 530 540 550
SLFGKGSQGA SYACFEKVNS PRENLANCDS KDSYSVPCGQ QDVLCGKLAC
560 570 580 590 600
FRPPKNYKSP SQSVVYSYVH DSVCLSILPG LSMRSDGRDS AYVADGTVCG
610 620 630 640 650
PQMYCINGTC KEVNFTGNDC NATKKCKGNG ICNNFGNCQC FPDYRPPDCN
660 670 680 690 700
LQIGSPGGSI DDGNTLRTES AFATKRLSKN EDSWVILGFF IFLPFIVTFL
710
VGIMKRNERK IVPQGEHKI
Length:719
Mass (Da):79,210
Last modified:July 27, 2011 - v2
Checksum:i9CD092E12625DA6D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451D → N in AAD48844 (PubMed:10395895).Curated
Sequence conflicti159 – 1591I → T in AAD48844 (PubMed:10395895).Curated
Sequence conflicti169 – 1691E → D in AAD48844 (PubMed:10395895).Curated
Sequence conflicti276 – 2761R → H in AAD48844 (PubMed:10395895).Curated
Sequence conflicti386 – 3861N → K in AAD48844 (PubMed:10395895).Curated
Sequence conflicti577 – 5771I → V in AAD48844 (PubMed:10395895).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167405 mRNA. Translation: AAD48844.1.
BC051136 mRNA. Translation: AAH51136.2.
U06148 mRNA. Translation: AAA18427.1.
CCDSiCCDS22195.1.
PIRiI48946.
RefSeqiNP_034214.2. NM_010084.2.
UniGeneiMm.89943.

Genome annotation databases

EnsembliENSMUST00000033957; ENSMUSP00000033957; ENSMUSG00000031552.
GeneIDi13524.
KEGGimmu:13524.
UCSCiuc009lfb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167405 mRNA. Translation: AAD48844.1.
BC051136 mRNA. Translation: AAH51136.2.
U06148 mRNA. Translation: AAA18427.1.
CCDSiCCDS22195.1.
PIRiI48946.
RefSeqiNP_034214.2. NM_010084.2.
UniGeneiMm.89943.

3D structure databases

ProteinModelPortaliQ9R157.
SMRiQ9R157. Positions 182-650.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033957.

Protein family/group databases

MEROPSiM12.958.

PTM databases

PhosphoSiteiQ9R157.

Proteomic databases

PaxDbiQ9R157.
PRIDEiQ9R157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033957; ENSMUSP00000033957; ENSMUSG00000031552.
GeneIDi13524.
KEGGimmu:13524.
UCSCiuc009lfb.2. mouse.

Organism-specific databases

CTDi8749.
MGIiMGI:105986. Adam18.

Phylogenomic databases

eggNOGiNOG329803.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ9R157.
KOiK16909.
OMAiDYMGSEM.
OrthoDBiEOG7W1537.
TreeFamiTF314733.

Miscellaneous databases

NextBioi284100.
PROiQ9R157.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R157.
CleanExiMM_ADAM18.
ExpressionAtlasiQ9R157. baseline and differential.
GenevisibleiQ9R157. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR000742. EG-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization."
    Zhu G.-Z., Lin Y., Myles D.G., Primakoff P.
    Gene 234:227-237(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. "A family of cellular proteins related to snake venom disintegrins."
    Weskamp G., Blobel C.P.
    Proc. Natl. Acad. Sci. U.S.A. 91:2748-2751(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 405-453.
    Strain: BALB/c.

Entry informationi

Entry nameiADA18_MOUSE
AccessioniPrimary (citable) accession number: Q9R157
Secondary accession number(s): Q60621, Q80Y08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.