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Q9R157

- ADA18_MOUSE

UniProt

Q9R157 - ADA18_MOUSE

Protein

Disintegrin and metalloproteinase domain-containing protein 18

Gene

Adam18

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein.

    GO - Molecular functioni

    1. integrin binding Source: MGI
    2. metalloendopeptidase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. spermatogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Protein family/group databases

    MEROPSiM12.958.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 18
    Short name:
    ADAM 18
    Alternative name(s):
    Disintegrin and metalloproteinase domain-containing protein 27
    Short name:
    ADAM 27
    Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
    Short name:
    tMDC III
    Gene namesi
    Name:Adam18
    Synonyms:Adam27, Dtgn3, Tmdc3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:105986. Adam18.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 179160Sequence AnalysisPRO_0000029100Add
    BLAST
    Chaini180 – 719540Disintegrin and metalloproteinase domain-containing protein 18PRO_0000029101Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi289 ↔ 373By similarity
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi332 ↔ 357By similarity
    Disulfide bondi334 ↔ 339By similarity
    Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi447 ↔ 468By similarity
    Glycosylationi607 – 6071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi614 – 6141N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi620 ↔ 632By similarity
    Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi626 ↔ 638By similarity
    Disulfide bondi640 ↔ 649By similarity

    Post-translational modificationi

    The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9R157.
    PRIDEiQ9R157.

    PTM databases

    PhosphoSiteiQ9R157.

    Expressioni

    Tissue specificityi

    Expressed specifically in testis.

    Developmental stagei

    Adult levels are reached by day 16 after birth.

    Gene expression databases

    BgeeiQ9R157.
    CleanExiMM_ADAM18.
    GenevestigatoriQ9R157.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000033957.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R157.
    SMRiQ9R157. Positions 182-650.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini173 – 683511ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini705 – 71915CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei684 – 70421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini180 – 378199Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini387 – 47690DisintegrinPROSITE-ProRule annotationAdd
    BLAST
    Domaini616 – 65035EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi477 – 615139Cys-richAdd
    BLAST

    Domaini

    A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.

    Sequence similaritiesi

    Contains 1 disintegrin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG329803.
    GeneTreeiENSGT00750000117474.
    HOGENOMiHOG000230883.
    HOVERGENiHBG103628.
    InParanoidiQ80Y08.
    KOiK16909.
    OMAiDYMGSEM.
    OrthoDBiEOG7W1537.
    TreeFamiTF314733.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiIPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR000742. EG-like_dom.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view]
    PfamiPF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view]
    SMARTiSM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9R157-1 [UniParc]FASTAAdd to Basket

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    MPLLFILAEL AMLFARLDSE GICLHITVPQ KIEPRKGGDA EGKVTYVITI    50
    DGKPYSLHLR NHSFLSQNFL VYTYNETGSL YSDSSHFLAH CHYRGYVDEV 100
    PNSIVTLSIC SGLRGFLQLE NVSYGIEPLE SSARFEHIVY QVKSDSSMLA 150
    GNDSHVWQID QLDKGHFNEQ DKNHSQLLPQ SLKLHIIVGK FLFDYMGSDI 200
    MAITQKIFQI IGLVNAMLTQ LKLSVVLASL ELWSDKNHIS TDGNATDILQ 250
    RLLDWKRDYL TLQSNEITHL LIYRRRPKYI GAASPGEICS KSYVAGVGMY 300
    PEDIGLEGFS VVITQLIGLH IGLTYDDNIR NCSCPSAPCI MQQGALSSSG 350
    KKTFSNCSLH DYMHYVSNFD TQCLGDLSNV HVLQPNQAVC GNGIMEAGEE 400
    CDCGNETECQ FKECCDHETC RLKGSAQCGS GACCMPTCEL SASGTPCRKA 450
    VDPECDFTEY CDGSSSHCVP DTFALNGHLC RLGSAYCYNG RCQALNDQCV 500
    SLFGKGSQGA SYACFEKVNS PRENLANCDS KDSYSVPCGQ QDVLCGKLAC 550
    FRPPKNYKSP SQSVVYSYVH DSVCLSILPG LSMRSDGRDS AYVADGTVCG 600
    PQMYCINGTC KEVNFTGNDC NATKKCKGNG ICNNFGNCQC FPDYRPPDCN 650
    LQIGSPGGSI DDGNTLRTES AFATKRLSKN EDSWVILGFF IFLPFIVTFL 700
    VGIMKRNERK IVPQGEHKI 719
    Length:719
    Mass (Da):79,210
    Last modified:July 27, 2011 - v2
    Checksum:i9CD092E12625DA6D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1451D → N in AAD48844. (PubMed:10395895)Curated
    Sequence conflicti159 – 1591I → T in AAD48844. (PubMed:10395895)Curated
    Sequence conflicti169 – 1691E → D in AAD48844. (PubMed:10395895)Curated
    Sequence conflicti276 – 2761R → H in AAD48844. (PubMed:10395895)Curated
    Sequence conflicti386 – 3861N → K in AAD48844. (PubMed:10395895)Curated
    Sequence conflicti577 – 5771I → V in AAD48844. (PubMed:10395895)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF167405 mRNA. Translation: AAD48844.1.
    BC051136 mRNA. Translation: AAH51136.2.
    U06148 mRNA. Translation: AAA18427.1.
    CCDSiCCDS22195.1.
    PIRiI48946.
    RefSeqiNP_034214.2. NM_010084.2.
    UniGeneiMm.89943.

    Genome annotation databases

    EnsembliENSMUST00000033957; ENSMUSP00000033957; ENSMUSG00000031552.
    GeneIDi13524.
    KEGGimmu:13524.
    UCSCiuc009lfb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF167405 mRNA. Translation: AAD48844.1 .
    BC051136 mRNA. Translation: AAH51136.2 .
    U06148 mRNA. Translation: AAA18427.1 .
    CCDSi CCDS22195.1.
    PIRi I48946.
    RefSeqi NP_034214.2. NM_010084.2.
    UniGenei Mm.89943.

    3D structure databases

    ProteinModelPortali Q9R157.
    SMRi Q9R157. Positions 182-650.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000033957.

    Protein family/group databases

    MEROPSi M12.958.

    PTM databases

    PhosphoSitei Q9R157.

    Proteomic databases

    PaxDbi Q9R157.
    PRIDEi Q9R157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033957 ; ENSMUSP00000033957 ; ENSMUSG00000031552 .
    GeneIDi 13524.
    KEGGi mmu:13524.
    UCSCi uc009lfb.2. mouse.

    Organism-specific databases

    CTDi 8749.
    MGIi MGI:105986. Adam18.

    Phylogenomic databases

    eggNOGi NOG329803.
    GeneTreei ENSGT00750000117474.
    HOGENOMi HOG000230883.
    HOVERGENi HBG103628.
    InParanoidi Q80Y08.
    KOi K16909.
    OMAi DYMGSEM.
    OrthoDBi EOG7W1537.
    TreeFami TF314733.

    Miscellaneous databases

    NextBioi 284100.
    PROi Q9R157.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9R157.
    CleanExi MM_ADAM18.
    Genevestigatori Q9R157.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProi IPR006586. ADAM_Cys-rich.
    IPR001762. Blood-coag_inhib_Disintegrin.
    IPR000742. EG-like_dom.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    [Graphical view ]
    Pfami PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    [Graphical view ]
    SMARTi SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57552. SSF57552. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization."
      Zhu G.-Z., Lin Y., Myles D.G., Primakoff P.
      Gene 234:227-237(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    3. "A family of cellular proteins related to snake venom disintegrins."
      Weskamp G., Blobel C.P.
      Proc. Natl. Acad. Sci. U.S.A. 91:2748-2751(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 405-453.
      Strain: BALB/c.

    Entry informationi

    Entry nameiADA18_MOUSE
    AccessioniPrimary (citable) accession number: Q9R157
    Secondary accession number(s): Q60621, Q80Y08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3