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Q9R157 (ADA18_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 18
Short name=ADAM 18
Alternative name(s):
Disintegrin and metalloproteinase domain-containing protein 27
Short name=ADAM 27
Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
Short name=tMDC III
Gene names
Name:Adam18
Synonyms:Adam27, Dtgn3, Tmdc3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length719 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed specifically in testis.

Developmental stage

Adult levels are reached by day 16 after birth.

Domain

A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.

Post-translational modification

The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 179160 Potential
PRO_0000029100
Chain180 – 719540Disintegrin and metalloproteinase domain-containing protein 18
PRO_0000029101

Regions

Topological domain173 – 683511Extracellular Potential
Transmembrane684 – 70421Helical; Potential
Topological domain705 – 71915Cytoplasmic Potential
Domain180 – 378199Peptidase M12B
Domain387 – 47690Disintegrin
Domain616 – 65035EGF-like
Compositional bias477 – 615139Cys-rich

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation1211N-linked (GlcNAc...) Potential
Glycosylation1521N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation2441N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation3561N-linked (GlcNAc...) Potential
Glycosylation4051N-linked (GlcNAc...) Potential
Glycosylation6071N-linked (GlcNAc...) Potential
Glycosylation6141N-linked (GlcNAc...) Potential
Glycosylation6211N-linked (GlcNAc...) Potential
Disulfide bond289 ↔ 373 By similarity
Disulfide bond332 ↔ 357 By similarity
Disulfide bond334 ↔ 339 By similarity
Disulfide bond447 ↔ 468 By similarity
Disulfide bond620 ↔ 632 By similarity
Disulfide bond626 ↔ 638 By similarity
Disulfide bond640 ↔ 649 By similarity

Experimental info

Sequence conflict1451D → N in AAD48844. Ref.1
Sequence conflict1591I → T in AAD48844. Ref.1
Sequence conflict1691E → D in AAD48844. Ref.1
Sequence conflict2761R → H in AAD48844. Ref.1
Sequence conflict3861N → K in AAD48844. Ref.1
Sequence conflict5771I → V in AAD48844. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9R157 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 9CD092E12625DA6D

FASTA71979,210
        10         20         30         40         50         60 
MPLLFILAEL AMLFARLDSE GICLHITVPQ KIEPRKGGDA EGKVTYVITI DGKPYSLHLR 

        70         80         90        100        110        120 
NHSFLSQNFL VYTYNETGSL YSDSSHFLAH CHYRGYVDEV PNSIVTLSIC SGLRGFLQLE 

       130        140        150        160        170        180 
NVSYGIEPLE SSARFEHIVY QVKSDSSMLA GNDSHVWQID QLDKGHFNEQ DKNHSQLLPQ 

       190        200        210        220        230        240 
SLKLHIIVGK FLFDYMGSDI MAITQKIFQI IGLVNAMLTQ LKLSVVLASL ELWSDKNHIS 

       250        260        270        280        290        300 
TDGNATDILQ RLLDWKRDYL TLQSNEITHL LIYRRRPKYI GAASPGEICS KSYVAGVGMY 

       310        320        330        340        350        360 
PEDIGLEGFS VVITQLIGLH IGLTYDDNIR NCSCPSAPCI MQQGALSSSG KKTFSNCSLH 

       370        380        390        400        410        420 
DYMHYVSNFD TQCLGDLSNV HVLQPNQAVC GNGIMEAGEE CDCGNETECQ FKECCDHETC 

       430        440        450        460        470        480 
RLKGSAQCGS GACCMPTCEL SASGTPCRKA VDPECDFTEY CDGSSSHCVP DTFALNGHLC 

       490        500        510        520        530        540 
RLGSAYCYNG RCQALNDQCV SLFGKGSQGA SYACFEKVNS PRENLANCDS KDSYSVPCGQ 

       550        560        570        580        590        600 
QDVLCGKLAC FRPPKNYKSP SQSVVYSYVH DSVCLSILPG LSMRSDGRDS AYVADGTVCG 

       610        620        630        640        650        660 
PQMYCINGTC KEVNFTGNDC NATKKCKGNG ICNNFGNCQC FPDYRPPDCN LQIGSPGGSI 

       670        680        690        700        710 
DDGNTLRTES AFATKRLSKN EDSWVILGFF IFLPFIVTFL VGIMKRNERK IVPQGEHKI

« Hide

References

« Hide 'large scale' references
[1]"Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization."
Zhu G.-Z., Lin Y., Myles D.G., Primakoff P.
Gene 234:227-237(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[3]"A family of cellular proteins related to snake venom disintegrins."
Weskamp G., Blobel C.P.
Proc. Natl. Acad. Sci. U.S.A. 91:2748-2751(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 405-453.
Strain: BALB/c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF167405 mRNA. Translation: AAD48844.1.
BC051136 mRNA. Translation: AAH51136.2.
U06148 mRNA. Translation: AAA18427.1.
IPIIPI00128143.
PIRI48946.
RefSeqNP_034214.2. NM_010084.2.
UniGeneMm.89943.

3D structure databases

ProteinModelPortalQ9R157.
SMRQ9R157. Positions 182-650.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000033957.

Protein family/group databases

MEROPSM12.958.

PTM databases

PhosphoSiteQ9R157.

Proteomic databases

PaxDbQ9R157.
PRIDEQ9R157.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033957; ENSMUSP00000033957; ENSMUSG00000031552.
GeneID13524.
KEGGmmu:13524.

Organism-specific databases

CTD8749.
MGIMGI:105986. Adam18.

Phylogenomic databases

eggNOGNOG329803.
GeneTreeENSGT00680000099710.
HOGENOMHOG000230883.
HOVERGENHBG103628.
InParanoidQ80Y08.
OMAKDVLCGK.
OrthoDBEOG4P8FHF.

Gene expression databases

ArrayExpressQ9R157.
BgeeQ9R157.
CleanExMM_ADAM18.
GenevestigatorQ9R157.
GermOnlineENSMUSG00000031552. Mus musculus.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR000742. EG-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMSSF57552. Disintegrin. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. False negative.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284100.
SOURCESearch...

Entry information

Entry nameADA18_MOUSE
AccessionPrimary (citable) accession number: Q9R157
Secondary accession number(s): Q60621, Q80Y08
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents