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Protein

Disintegrin and metalloproteinase domain-containing protein 18

Gene

Adam18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein.

GO - Molecular functioni

  • integrin binding Source: MGI
  • metalloendopeptidase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Protein family/group databases

MEROPSiM12.958.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 18
Short name:
ADAM 18
Alternative name(s):
Disintegrin and metalloproteinase domain-containing protein 27
Short name:
ADAM 27
Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
Short name:
tMDC III
Gene namesi
Name:Adam18
Synonyms:Adam27, Dtgn3, Tmdc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:105986. Adam18.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini173 – 683ExtracellularSequence analysisAdd BLAST511
Transmembranei684 – 704HelicalSequence analysisAdd BLAST21
Topological domaini705 – 719CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002910020 – 179Sequence analysisAdd BLAST160
ChainiPRO_0000029101180 – 719Disintegrin and metalloproteinase domain-containing protein 18Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi121N-linked (GlcNAc...)Sequence analysis1
Glycosylationi152N-linked (GlcNAc...)Sequence analysis1
Glycosylationi173N-linked (GlcNAc...)Sequence analysis1
Glycosylationi244N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi289 ↔ 373By similarity
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi332 ↔ 357By similarity
Disulfide bondi334 ↔ 339By similarity
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1
Glycosylationi405N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi447 ↔ 468By similarity
Glycosylationi607N-linked (GlcNAc...)Sequence analysis1
Glycosylationi614N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi620 ↔ 632By similarity
Glycosylationi621N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi626 ↔ 638By similarity
Disulfide bondi640 ↔ 649By similarity

Post-translational modificationi

The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9R157.
PRIDEiQ9R157.

PTM databases

iPTMnetiQ9R157.
PhosphoSitePlusiQ9R157.

Expressioni

Tissue specificityi

Expressed specifically in testis.

Developmental stagei

Adult levels are reached by day 16 after birth.

Gene expression databases

BgeeiENSMUSG00000031552.
CleanExiMM_ADAM18.
ExpressionAtlasiQ9R157. baseline and differential.
GenevisibleiQ9R157. MM.

Interactioni

GO - Molecular functioni

  • integrin binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033957.

Structurei

3D structure databases

ProteinModelPortaliQ9R157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 378Peptidase M12BPROSITE-ProRule annotationAdd BLAST199
Domaini387 – 476DisintegrinPROSITE-ProRule annotationAdd BLAST90
Domaini616 – 650EGF-likePROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi477 – 615Cys-richAdd BLAST139

Domaini

A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00800000124044.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ9R157.
KOiK16909.
OMAiYMGSEMM.
OrthoDBiEOG091G02F0.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Disintegrin_dom.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLFILAEL AMLFARLDSE GICLHITVPQ KIEPRKGGDA EGKVTYVITI
60 70 80 90 100
DGKPYSLHLR NHSFLSQNFL VYTYNETGSL YSDSSHFLAH CHYRGYVDEV
110 120 130 140 150
PNSIVTLSIC SGLRGFLQLE NVSYGIEPLE SSARFEHIVY QVKSDSSMLA
160 170 180 190 200
GNDSHVWQID QLDKGHFNEQ DKNHSQLLPQ SLKLHIIVGK FLFDYMGSDI
210 220 230 240 250
MAITQKIFQI IGLVNAMLTQ LKLSVVLASL ELWSDKNHIS TDGNATDILQ
260 270 280 290 300
RLLDWKRDYL TLQSNEITHL LIYRRRPKYI GAASPGEICS KSYVAGVGMY
310 320 330 340 350
PEDIGLEGFS VVITQLIGLH IGLTYDDNIR NCSCPSAPCI MQQGALSSSG
360 370 380 390 400
KKTFSNCSLH DYMHYVSNFD TQCLGDLSNV HVLQPNQAVC GNGIMEAGEE
410 420 430 440 450
CDCGNETECQ FKECCDHETC RLKGSAQCGS GACCMPTCEL SASGTPCRKA
460 470 480 490 500
VDPECDFTEY CDGSSSHCVP DTFALNGHLC RLGSAYCYNG RCQALNDQCV
510 520 530 540 550
SLFGKGSQGA SYACFEKVNS PRENLANCDS KDSYSVPCGQ QDVLCGKLAC
560 570 580 590 600
FRPPKNYKSP SQSVVYSYVH DSVCLSILPG LSMRSDGRDS AYVADGTVCG
610 620 630 640 650
PQMYCINGTC KEVNFTGNDC NATKKCKGNG ICNNFGNCQC FPDYRPPDCN
660 670 680 690 700
LQIGSPGGSI DDGNTLRTES AFATKRLSKN EDSWVILGFF IFLPFIVTFL
710
VGIMKRNERK IVPQGEHKI
Length:719
Mass (Da):79,210
Last modified:July 27, 2011 - v2
Checksum:i9CD092E12625DA6D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145D → N in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti159I → T in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti169E → D in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti276R → H in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti386N → K in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti577I → V in AAD48844 (PubMed:10395895).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167405 mRNA. Translation: AAD48844.1.
BC051136 mRNA. Translation: AAH51136.2.
U06148 mRNA. Translation: AAA18427.1.
CCDSiCCDS22195.1.
PIRiI48946.
RefSeqiNP_034214.2. NM_010084.2.
UniGeneiMm.89943.

Genome annotation databases

EnsembliENSMUST00000033957; ENSMUSP00000033957; ENSMUSG00000031552.
GeneIDi13524.
KEGGimmu:13524.
UCSCiuc009lfb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167405 mRNA. Translation: AAD48844.1.
BC051136 mRNA. Translation: AAH51136.2.
U06148 mRNA. Translation: AAA18427.1.
CCDSiCCDS22195.1.
PIRiI48946.
RefSeqiNP_034214.2. NM_010084.2.
UniGeneiMm.89943.

3D structure databases

ProteinModelPortaliQ9R157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033957.

Protein family/group databases

MEROPSiM12.958.

PTM databases

iPTMnetiQ9R157.
PhosphoSitePlusiQ9R157.

Proteomic databases

PaxDbiQ9R157.
PRIDEiQ9R157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033957; ENSMUSP00000033957; ENSMUSG00000031552.
GeneIDi13524.
KEGGimmu:13524.
UCSCiuc009lfb.2. mouse.

Organism-specific databases

CTDi8749.
MGIiMGI:105986. Adam18.

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00800000124044.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ9R157.
KOiK16909.
OMAiYMGSEMM.
OrthoDBiEOG091G02F0.
TreeFamiTF314733.

Miscellaneous databases

PROiQ9R157.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031552.
CleanExiMM_ADAM18.
ExpressionAtlasiQ9R157. baseline and differential.
GenevisibleiQ9R157. MM.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Disintegrin_dom.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADA18_MOUSE
AccessioniPrimary (citable) accession number: Q9R157
Secondary accession number(s): Q60621, Q80Y08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.