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Protein

Protein arginine N-methyltransferase 2

Gene

Prmt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. May inhibit NF-kappa-B transcription, and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). Has a negative regulation effect on G1 to S transition of mitotic cell cycle. Involved in growth regulation. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation.4 Publications

Catalytic activityi

2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega)-dimethyl-L-arginine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei124 – 1241S-adenosyl-L-methionineBy similarity
Binding sitei133 – 1331S-adenosyl-L-methionineBy similarity
Binding sitei157 – 1571S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei180 – 1801S-adenosyl-L-methionineBy similarity
Binding sitei209 – 2091S-adenosyl-L-methionineBy similarity
Active sitei223 – 2231By similarity
Active sitei232 – 2321By similarity

GO - Molecular functioni

GO - Biological processi

  • developmental cell growth Source: UniProtKB
  • histone methylation Source: UniProtKB
  • leptin-mediated signaling pathway Source: BHF-UCL
  • methylation Source: BHF-UCL
  • negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
  • negative regulation of NF-kappaB transcription factor activity Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of androgen receptor signaling pathway Source: UniProtKB
  • regulation of tyrosine phosphorylation of Stat3 protein Source: BHF-UCL
  • response to hypoxia Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 2 (EC:2.1.1.319By similarity)
Alternative name(s):
Histone-arginine N-methyltransferase PRMT2
Gene namesi
Name:Prmt2
Synonyms:Hrmt1l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1316652. Prmt2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Translocates from the cytoplasm to the nucleus, after hormone exposure.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

From 6 to 30 weeks of age, males gain less weight than age-matched control wild-type mice when weaned onto a chow diet. By 30 weeks of age, females are also lean compared with wild-type females. At 12 weeks of age, male and female are 3% to 4% shorter than wild-type controls. Shows less hepatic cytoplasmic vacuoles, more distinct hepatic cords and sinusoids and increased leptin sensitivity.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Protein arginine N-methyltransferase 2PRO_0000212325Add
BLAST

Proteomic databases

MaxQBiQ9R144.
PaxDbiQ9R144.
PRIDEiQ9R144.

PTM databases

iPTMnetiQ9R144.
PhosphoSiteiQ9R144.

Expressioni

Tissue specificityi

Expressed in liver, pancreas, lung, brain, skeletal muscle, heart, muscle and fat.1 Publication

Gene expression databases

CleanExiMM_PRMT2.

Interactioni

Subunit structurei

Self-associates (By similarity). Interacts with HNRNPUL1. Interacts with NFKBIA (By similarity). Interacts with NCOA6 coactivator. Interacts (via SH3 domain) with PRMT8. Interacts with AR. Interacts with ESR1, ESR2, PGR, PPARG, RARA, RXRA and THRB (By similarity). Interacts with RB1 and E2F1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9R144. 1 interaction.
STRINGi10090.ENSMUSP00000020452.

Structurei

3D structure databases

ProteinModelPortaliQ9R144.
SMRiQ9R144. Positions 45-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 10160SH3PROSITE-ProRule annotationAdd
BLAST
Domaini111 – 414304SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 289289Interaction with ESR1By similarityAdd
BLAST
Regioni95 – 219125Interaction with RB1Add
BLAST
Regioni145 – 287143Interaction with ESR1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase PRMT-type domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG1499. Eukaryota.
ENOG410XQYH. LUCA.
HOGENOMiHOG000198521.
HOVERGENiHBG105734.
InParanoidiQ9R144.
PhylomeDBiQ9R144.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR029063. SAM-dependent_MTases.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR11006. PTHR11006. 1 hit.
PfamiPF05185. PRMT5. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPGEGPCS ESQVIPVLEE DPVDYGCEMQ LLQDGAQLQL QLQPEEFVAI
60 70 80 90 100
ADYTATDETQ LSFLRGEKIL ILRQTTADWW WGERAGCCGY IPANHLGKQL
110 120 130 140 150
EEYDPEDTWQ DEEYFDSYGT LKLHLEMLAD QPRTTKYHSV ILQNKESLKD
160 170 180 190 200
KVILDVGCGT GIISLFCAHH ARPKAVYAVE ASDMAQHTSQ LVLQNGFADT
210 220 230 240 250
ITVFQQKVED VVLPEKVDVL VSEWMGTCLL FEFMIESILY ARDTWLKGDG
260 270 280 290 300
IIWPTTAALH LVPCSAEKDY HSKVLFWDNA YEFNLSALKS LAIKEFFSRP
310 320 330 340 350
KSNHILKPED CLSEPCTILQ LDMRTVQVPD LETMRGELRF DIQKAGTLHG
360 370 380 390 400
FTAWFSVYFQ SLEEGQPQQV VSTGPLHPTT HWKQTLFMMD DPVPVHTGDV
410 420 430 440
VHGFCCVTKK SGMEKAHVCL SELGCHVRTR SHVSTELETG SFRSGGDS
Length:448
Mass (Da):50,476
Last modified:May 1, 2000 - v1
Checksum:i63E1F8C3FC66C25E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169620 mRNA. Translation: AAD48847.1.
UniGeneiMm.32020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169620 mRNA. Translation: AAD48847.1.
UniGeneiMm.32020.

3D structure databases

ProteinModelPortaliQ9R144.
SMRiQ9R144. Positions 45-431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R144. 1 interaction.
STRINGi10090.ENSMUSP00000020452.

PTM databases

iPTMnetiQ9R144.
PhosphoSiteiQ9R144.

Proteomic databases

MaxQBiQ9R144.
PaxDbiQ9R144.
PRIDEiQ9R144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1316652. Prmt2.

Phylogenomic databases

eggNOGiKOG1499. Eukaryota.
ENOG410XQYH. LUCA.
HOGENOMiHOG000198521.
HOVERGENiHBG105734.
InParanoidiQ9R144.
PhylomeDBiQ9R144.

Miscellaneous databases

PROiQ9R144.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PRMT2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR029063. SAM-dependent_MTases.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR11006. PTHR11006. 1 hit.
PfamiPF05185. PRMT5. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANM2_MOUSE
AccessioniPrimary (citable) accession number: Q9R144
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.