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Protein

Guanine deaminase

Gene

Gda

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.

Catalytic activityi

Guanine + H2O = xanthine + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: guanine degradation

This protein is involved in step 1 of the subpathway that synthesizes xanthine from guanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Guanine deaminase (Gda)
This subpathway is part of the pathway guanine degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes xanthine from guanine, the pathway guanine degradation and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Zinc; via tele nitrogenBy similarity
Metal bindingi84 – 841Zinc; via tele nitrogenBy similarity
Binding sitei87 – 871SubstrateBy similarity
Binding sitei213 – 2131SubstrateBy similarity
Metal bindingi240 – 2401Zinc; via tele nitrogenBy similarity
Binding sitei243 – 2431SubstrateBy similarity
Binding sitei279 – 2791SubstrateBy similarity
Metal bindingi330 – 3301ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-74259. Purine catabolism.
SABIO-RKQ9R111.
UniPathwayiUPA00603; UER00660.

Protein family/group databases

MEROPSiM38.981.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine deaminase (EC:3.5.4.3)
Short name:
Guanase
Short name:
Guanine aminase
Alternative name(s):
Guanine aminohydrolase
Short name:
GAH
Gene namesi
Name:Gda
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:95678. Gda.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Guanine deaminasePRO_0000122299Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei453 – 4531PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9R111.
MaxQBiQ9R111.
PaxDbiQ9R111.
PRIDEiQ9R111.

2D gel databases

REPRODUCTION-2DPAGEIPI00469987.
Q9R111.

PTM databases

iPTMnetiQ9R111.
PhosphoSiteiQ9R111.
SwissPalmiQ9R111.

Expressioni

Gene expression databases

BgeeiQ9R111.
CleanExiMM_GDA.
ExpressionAtlasiQ9R111. baseline and differential.
GenevisibleiQ9R111. MM.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4Q621085EBI-2308876,EBI-300895

Protein-protein interaction databases

IntActiQ9R111. 2 interactions.
MINTiMINT-4097083.
STRINGi10090.ENSMUSP00000084882.

Structurei

3D structure databases

ProteinModelPortaliQ9R111.
SMRiQ9R111. Positions 8-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATZ/TRZ family.Curated

Phylogenomic databases

eggNOGiKOG3968. Eukaryota.
COG0402. LUCA.
GeneTreeiENSGT00390000017130.
HOVERGENiHBG005930.
InParanoidiQ9R111.
KOiK01487.
OMAiDFFGDIS.
OrthoDBiEOG744T91.
TreeFamiTF324539.

Family and domain databases

InterProiIPR006680. Amidohydro-rel.
IPR014311. Guanine_deaminase.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11271:SF6. PTHR11271:SF6. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR02967. guan_deamin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9R111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAARTPPLA LVFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEESSQ
60 70 80 90 100
QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHAPQYAF AGSNVDLPLL
110 120 130 140 150
EWLNKYTFPT EQRFRSTDVA EEVYTRVVRR TLKNGTTTAC YFGTIHTDSS
160 170 180 190 200
LILAEITDKF GQRAFVGKVC MDLNDTVPEY KETTEESVKE TERFVSEMLQ
210 220 230 240 250
KNYPRVKPIV TPRFTLSCTE TLMSELGNIA KTHDLYIQSH ISENREEIEA
260 270 280 290 300
VKSLYPSYKN YTDVYDKNNL LTNKTVMAHG CYLSEEELNI FSERGASIAH
310 320 330 340 350
CPNSNLSLSS GLLNVLEVLK HKVKIGLGTD VAGGYSYSML DAIRRAVMVS
360 370 380 390 400
NVLLINKVNE KNLTLKEVFR LATLGGSQAL GLDSEIGNFE VGKEFDALLI
410 420 430 440 450
NPRASDSPID LFYGDFVGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF

SSSV
Length:454
Mass (Da):51,013
Last modified:May 1, 2000 - v1
Checksum:i7711573C8C93D64E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174583 mRNA. Translation: AAD50297.1.
CCDSiCCDS29698.1.
RefSeqiNP_034396.1. NM_010266.2.
UniGeneiMm.45054.

Genome annotation databases

EnsembliENSMUST00000087600; ENSMUSP00000084882; ENSMUSG00000058624.
GeneIDi14544.
KEGGimmu:14544.
UCSCiuc008gyx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174583 mRNA. Translation: AAD50297.1.
CCDSiCCDS29698.1.
RefSeqiNP_034396.1. NM_010266.2.
UniGeneiMm.45054.

3D structure databases

ProteinModelPortaliQ9R111.
SMRiQ9R111. Positions 8-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R111. 2 interactions.
MINTiMINT-4097083.
STRINGi10090.ENSMUSP00000084882.

Protein family/group databases

MEROPSiM38.981.

PTM databases

iPTMnetiQ9R111.
PhosphoSiteiQ9R111.
SwissPalmiQ9R111.

2D gel databases

REPRODUCTION-2DPAGEIPI00469987.
Q9R111.

Proteomic databases

EPDiQ9R111.
MaxQBiQ9R111.
PaxDbiQ9R111.
PRIDEiQ9R111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087600; ENSMUSP00000084882; ENSMUSG00000058624.
GeneIDi14544.
KEGGimmu:14544.
UCSCiuc008gyx.1. mouse.

Organism-specific databases

CTDi9615.
MGIiMGI:95678. Gda.

Phylogenomic databases

eggNOGiKOG3968. Eukaryota.
COG0402. LUCA.
GeneTreeiENSGT00390000017130.
HOVERGENiHBG005930.
InParanoidiQ9R111.
KOiK01487.
OMAiDFFGDIS.
OrthoDBiEOG744T91.
TreeFamiTF324539.

Enzyme and pathway databases

UniPathwayiUPA00603; UER00660.
ReactomeiR-MMU-74259. Purine catabolism.
SABIO-RKQ9R111.

Miscellaneous databases

ChiTaRSiGda. mouse.
NextBioi286216.
PROiQ9R111.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R111.
CleanExiMM_GDA.
ExpressionAtlasiQ9R111. baseline and differential.
GenevisibleiQ9R111. MM.

Family and domain databases

InterProiIPR006680. Amidohydro-rel.
IPR014311. Guanine_deaminase.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11271:SF6. PTHR11271:SF6. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR02967. guan_deamin. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Paletzki R.F., Gerfen C.R.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Cloning and characterization of human guanine deaminase. Purification and partial amino acid sequence of the mouse protein."
    Yuan G., Bin J.C., McKay D.J., Snyder F.F.
    J. Biol. Chem. 274:8175-8180(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Strain: C57BL/6J.
    Tissue: Erythrocyte.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Liver, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiGUAD_MOUSE
AccessioniPrimary (citable) accession number: Q9R111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.