Q9R111 (GUAD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine deaminase
Short name=Guanase
Short name=Guanine aminase
EC=3.5.4.3

Alternative name(s):
Guanine aminohydrolase
Short name=GAH

Gene names

Name:

Gda

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
Catalytic activity	Guanine + H2O = xanthine + NH3.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the ATZ/TRZ family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	guanine deaminase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Dlg4	Q62108	5	EBI-2308876,EBI-300895

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Guanine deaminase		PRO_0000122299
Sites
	Metal binding	82	1	Zinc; via tele nitrogen By similarity
	Metal binding	84	1	Zinc; via tele nitrogen By similarity
	Metal binding	240	1	Zinc; via tele nitrogen By similarity
	Metal binding	330	1	Zinc By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	279	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9R111 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7711573C8C93D64E	FASTA	454	51,013
	10 20 30 40 50 60 MCAARTPPLA LVFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEESSQ QEKLAKEWCF 70 80 90 100 110 120 KPCEIRELSH HEFFMPGLVD THIHAPQYAF AGSNVDLPLL EWLNKYTFPT EQRFRSTDVA 130 140 150 160 170 180 EEVYTRVVRR TLKNGTTTAC YFGTIHTDSS LILAEITDKF GQRAFVGKVC MDLNDTVPEY 190 200 210 220 230 240 KETTEESVKE TERFVSEMLQ KNYPRVKPIV TPRFTLSCTE TLMSELGNIA KTHDLYIQSH 250 260 270 280 290 300 ISENREEIEA VKSLYPSYKN YTDVYDKNNL LTNKTVMAHG CYLSEEELNI FSERGASIAH 310 320 330 340 350 360 CPNSNLSLSS GLLNVLEVLK HKVKIGLGTD VAGGYSYSML DAIRRAVMVS NVLLINKVNE 370 380 390 400 410 420 KNLTLKEVFR LATLGGSQAL GLDSEIGNFE VGKEFDALLI NPRASDSPID LFYGDFVGDI 430 440 450 SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF SSSV

References

[1]	Paletzki R.F., Gerfen C.R. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"Cloning and characterization of human guanine deaminase. Purification and partial amino acid sequence of the mouse protein." Yuan G., Bin J.C., McKay D.J., Snyder F.F. J. Biol. Chem. 274:8175-8180(1999) [PubMed: 10075721] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: C57BL/6J. Tissue: Erythrocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF174583 mRNA. Translation: AAD50297.1.
IPI	IPI00469987.
RefSeq	NP_034396.1. NM_010266.2.
UniGene	Mm.45054.
3D structure databases
ProteinModelPortal	Q9R111.
SMR	Q9R111. Positions 8-451.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9R111. 1 interaction.
STRING	Q9R111.
Protein family/group databases
MEROPS	M38.981.
PTM databases
PhosphoSite	Q9R111.
2D gel databases
REPRODUCTION-2DPAGE	IPI00469987. Q9R111.
Proteomic databases
PRIDE	Q9R111.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000087600; ENSMUSP00000084882; ENSMUSG00000058624.
GeneID	14544.
KEGG	mmu:14544.
Organism-specific databases
CTD	9615.
MGI	MGI:95678. Gda.
Phylogenomic databases
GeneTree	ENSGT00390000017130.
HOVERGEN	HBG005930.
InParanoid	Q9R111.
OMA	CFKPCEI.
OrthoDB	EOG4ZKJMM.
PhylomeDB	Q9R111.
Gene expression databases
ArrayExpress	Q9R111.
Bgee	Q9R111.
CleanEx	MM_GDA.
Genevestigator	Q9R111.
GermOnline	ENSMUSG00000058624. Mus musculus.
Family and domain databases
InterPro	IPR006680. Amidohydro_1. IPR014311. Guanine_deaminase. [Graphical view]
KO	K01487.
PANTHER	PTHR11271:SF6. Guanine_deaminase. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR02967. Guan_deamin. 1 hit.
ProtoNet	Search...
Other
NextBio	286216.
SOURCE	Search...

Entry information

Entry name

GUAD_MOUSE

Accession

Primary (citable) accession number: Q9R111

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program