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Protein

Hormone-sensitive lipase

Gene

LIPE

Organism
Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus tridecemlineatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.By similarity

Catalytic activityi

Diacylglycerol + H2O = monoacylglycerol + a carboxylate.
Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Monoacylglycerol + H2O = glycerol + a carboxylate.

Enzyme regulationi

Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin (By similarity).By similarity

Pathwayi: triacylglycerol degradation

This protein is involved in the pathway triacylglycerol degradation, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol degradation and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei424PROSITE-ProRule annotation1
Active sitei692By similarity1
Active sitei722By similarity1

GO - Molecular functioni

  • hormone-sensitive lipase activity Source: UniProtKB-EC
  • lipase activity Source: InterPro
  • rRNA primary transcript binding Source: UniProtKB

GO - Biological processi

  • cholesterol metabolic process Source: UniProtKB-KW
  • lipid catabolic process Source: UniProtKB
  • termination of RNA polymerase I transcription Source: UniProtKB
  • transcription initiation from RNA polymerase I promoter Source: UniProtKB
  • triglyceride catabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
Biological processCholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism

Enzyme and pathway databases

UniPathwayiUPA00256.

Protein family/group databases

ESTHERispetr-hslip. Hormone-sensitive_lipase_like.
MEROPSiS09.993.

Names & Taxonomyi

Protein namesi
Recommended name:
Hormone-sensitive lipase (EC:3.1.1.79)
Short name:
HSL
Gene namesi
Name:LIPE
OrganismiIctidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus tridecemlineatus)
Taxonomic identifieri43179 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciuromorphaSciuridaeXerinaeMarmotiniIctidomys
Proteomesi
  • UP000005215 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000715511 – 763Hormone-sensitive lipaseAdd BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei552PhosphoserineBy similarity1
Modified residuei554Phosphoserine; by AMPKBy similarity1
Modified residuei595PhosphoserineBy similarity1
Modified residuei627PhosphoserineBy similarity1
Modified residuei649PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by AMPK may block translocation to lipid droplets.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5.By similarity

Protein-protein interaction databases

STRINGi43179.ENSSTOP00000010392.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi350 – 352Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity3

Sequence similaritiesi

Belongs to the 'GDXG' lipolytic enzyme family.Curated

Phylogenomic databases

eggNOGiKOG4388. Eukaryota.
COG0657. LUCA.
HOVERGENiHBG000187.
InParanoidiQ9R101.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR010468. HSL_N.
IPR002168. Lipase_GDXG_HIS_AS.
IPR033140. Lipase_GDXG_put_SER_AS.
PfamiView protein in Pfam
PF07859. Abhydrolase_3. 2 hits.
PF06350. HSL_N. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
PROSITEiView protein in PROSITE
PS01173. LIPASE_GDXG_HIS. 1 hit.
PS01174. LIPASE_GDXG_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9R101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLRTMTQSL VTLAEDNMAF FSSQGPGETA RRLSNVFAGV REQALGLEPA
60 70 80 90 100
LGSLLSVAHL FDLDPETPAN GYRSLVHTAR CCLAHLLHKS RYVASNRRSI
110 120 130 140 150
FFRTSHNLAE LEAYLAALTQ LRALAYYAQR LLATNRPGGL FFEGDEGLTA
160 170 180 190 200
DFLREYVTLH KGCFYGRCLG FQFTPSIRPF LQTISIGLVS FGEHYKRNES
210 220 230 240 250
GLGVTASSLF TSGRFAIDPE LRGAEFERIT QNLDVHFWKA FWNITEIEVL
260 270 280 290 300
SSLANMASAT VRVSRLISLP PEAFEMPLTS DPKLTVTISP PLAHTGPGPV
310 320 330 340 350
LIRLISYDLR EGQDSEELNS MVKSEGPRIL ELRPRPQQTS RSRSLVVXFH
360 370 380 390 400
GGGFVAQTSK SHEPYLKSWA QELGAPIISI DYSLAPEAPF PRALEECFFA
410 420 430 440 450
YCWAVKHCAL LGSTGERICL AGDSAGGNLC FTVALRAAAY GVRVPDGIMA
460 470 480 490 500
AYPATMLQSA ASPSRLLSLM DPLLPLSVLS KCVSAYAGAE TEEHPNSDEK
510 520 530 540 550
ALGMMGLVRR DTSLLLRDLR LGASSWLNSF LEFSGQKSQK TSAPTVESMR
560 570 580 590 600
RSVSEAALAQ PEGPVGTDSL KILTLKDLNL KSSSETSETP EMSLSVETLG
610 620 630 640 650
PSTPSDVNFF LRPEDAREEA EAKEGLSAKD GSSRVSNAFP EGFHPRRTSQ
660 670 680 690 700
GATQMPLYSS PIVKNPFMSP LLAPDNMLKT LPPVHIVACA LDPMLDDSXM
710 720 730 740 750
FARRLRALGQ PVTLRVVEDL PHGFLSLASL CRETRQAAEL CVERIRLILT
760
PPAAAAAAAP PPL
Length:763
Mass (Da):83,129
Last modified:May 1, 2000 - v1
Checksum:iE5516B02EFB8BE4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177401 mRNA. Translation: AAD51122.1.
RefSeqiNP_001269179.1. NM_001282250.1.

Genome annotation databases

GeneIDi101976389.

Similar proteinsi

Entry informationi

Entry nameiLIPS_ICTTR
AccessioniPrimary (citable) accession number: Q9R101
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: May 1, 2000
Last modified: October 25, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families