Reviewed, UniProtKB/Swiss-Prot Q9R0Y5 (KAD1_MOUSE)

Names and origin

Protein names

Recommended name:
    Adenylate kinase isoenzyme 1
      Short name=AK 1
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 1
    Myokinase

Gene names

Name:

Ak1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth By similarity. May provide a mechanism to buffer the adenylate energy charge for sperm motility.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Developmental stage	Up-regulated during late spermiogenesis, when the flagellum is being assembled. Ref.2
Sequence similarities	Belongs to the adenylate kinase family.
Sequence caution	The sequence CAM16612.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP metabolic process Inferred from electronic annotation. Source: InterPro cell cycle arrest Ref.1 Inferred from direct assay. Source: MGI
Cellular component	mitochondrion Inferred from direct assay. Source: MGI plasma membrane Ref.1 Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9R0Y5-1)

Also known as: Ak1a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9R0Y5-2)

Also known as: Ak1b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: ME → MGCCVSSEPQEEGGRKTG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Adenylate kinase isoenzyme 1		PRO_0000158911
Regions
	Nucleotide binding	15 – 23	9	ATP By similarity
	Nucleotide binding	94 – 101	8	AMP By similarity
Sites
	Binding site	39	1	AMP By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Natural variations
	Alternative sequence	1 – 2	2	ME → MGCCVSSEPQEEGGRKTG in isoform 2.		VSP_024843

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Ak1a) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 88E7862522967D14	FASTA	194	21,540
	10 20 30 40 50 60 MEEKLKKAKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG SERGKKLSAI 70 80 90 100 110 120 MEKGELVPLD TVLDMLRDAM LAKVDSSNGF LIDGYPREVK QGEEFEQKIG QPTLLLYVDA 130 140 150 160 170 180 GAETMTQRLL KRGETSGRVD DNEETIKKRL ETYYNATEPV ISFYDKRGIV RKVNAEGTVD 190 TVFSEVCTYL DSLK
	Isoform 2 (Ak1b). Checksum: 5295189A6E7CB6D8	FASTA	210	23,116
	10 20 30 40 50 60 MGCCVSSEPQ EEGGRKTGEK LKKAKIIFVV GGPGSGKGTQ CEKIVQKYGY THLSTGDLLR 70 80 90 100 110 120 AEVSSGSERG KKLSAIMEKG ELVPLDTVLD MLRDAMLAKV DSSNGFLIDG YPREVKQGEE 130 140 150 160 170 180 FEQKIGQPTL LLYVDAGAET MTQRLLKRGE TSGRVDDNEE TIKKRLETYY NATEPVISFY 190 200 210 DKRGIVRKVN AEGTVDTVFS EVCTYLDSLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"wt p53 dependent expression of a membrane-associated isoform of adenylate kinase." Collavin L., Lazarevic D., Utrera R., Marzinotto S., Monte M., Schneider C. Oncogene 18:5879-5888(1999) [PubMed: 10557075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	"Adenylate kinases 1 and 2 are part of the accessory structures in the mouse sperm flagellum." Cao W., Haig-Ladewig L., Gerton G.L., Moss S.B. Biol. Reprod. 75:492-500(2006) [PubMed: 16790685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DEVELOPMENTAL STAGE. Strain: CD-1.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Adipose tissue.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and FVB/N. Tissue: Mammary tumor and Retina.
[6]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 32-44; 64-77; 84-97 AND 150-166, MASS SPECTROMETRY. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ010108 mRNA. Translation: CAB52407.1. AJ010109 mRNA. Translation: CAB52408.1. DQ486026 mRNA. Translation: ABF46940.1. AK046613 mRNA. Translation: BAC32808.1. AK089270 mRNA. Translation: BAC40822.1. AL772271 Genomic DNA. Translation: CAM16612.1. Sequence problems. AL772271 Genomic DNA. Translation: CAM16613.1. AL772271 Genomic DNA. Translation: CAM16614.1. BC014802 mRNA. Translation: AAH14802.1. BC054366 mRNA. Translation: AAH54366.1.
IPI	IPI00128209. IPI00750256.
RefSeq	NP_067490.1.
UniGene	Mm.29189
3D structure databases
HSSP	HSSP built from PDB template 3ADK based on UniProtKB P00571.
SMR	Q9R0Y5. Positions 1-193.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9R0Y5.
2-D gel databases
REPRODUCTION-2DPAGE	Q9R0Y5.
Proteomic databases
PRIDE	Q9R0Y5.
Genome annotation databases
Ensembl	ENSMUST00000068271; ENSMUSP00000068479; ENSMUSG00000026817; Mus musculus. [Genome view] ENSMUST00000113277; ENSMUSP00000108902; ENSMUSG00000026817; Mus musculus. [Genome view] ENSMUST00000113278; ENSMUSP00000108903; ENSMUSG00000026817; Mus musculus. [Genome view]
GeneID	11636.
KEGG	mmu:11636.
NMPDR	fig|10090.3.peg.5814.
UCSC	uc008jgh.1. mouse. uc008jgj.1. mouse.
Organism-specific databases
MGI	MGI:87977. Ak1.
Phylogenomic databases
HOGENOM	Q9R0Y5.
HOVERGEN	Q9R0Y5.
OMA	GPETMTQ.
Enzyme and pathway databases
BRENDA	2.7.4.3. 244.
Gene expression databases
ArrayExpress	Q9R0Y5.
Bgee	Q9R0Y5.
CleanEx	MM_AK1.
Genevestigator	Q9R0Y5.
GermOnline	ENSMUSG00000026817. Mus musculus.
Family and domain databases
InterPro	IPR000850. Adenylate_kin. IPR006267. Adenylate_kin1. [Graphical view]
PANTHER	PTHR23359. Adenylate_kin. 1 hit.
Pfam	PF00406. ADK. 1 hit. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
ProDom	PD000657. Adenylate_kin. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01360. aden_kin_iso1. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

KAD1_MOUSE

Accession

Primary (citable) accession number: Q9R0Y5
Secondary accession number(s): A2AK80, Q542C5, Q9R0Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)