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Protein

Adenylate kinase isoenzyme 1

Gene

Ak1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity). May provide a mechanism to buffer the adenylate energy charge for sperm motility.By similarity1 Publication

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39AMPUniRule annotation1
Binding sitei44AMPUniRule annotation1
Binding sitei101AMPUniRule annotation1
Binding sitei132ATPUniRule annotation1
Binding sitei138AMPUniRule annotation1
Binding sitei149AMPUniRule annotation1
Binding sitei177ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 23ATPUniRule annotation6
Nucleotide bindingi65 – 67AMPUniRule annotation3
Nucleotide bindingi94 – 97AMPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.3. 3474.
ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 1UniRule annotation (EC:2.7.4.3UniRule annotation, EC:2.7.4.6UniRule annotation)
Short name:
AK 1UniRule annotation
Alternative name(s):
ATP-AMP transphosphorylase 1UniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
MyokinaseUniRule annotation
Gene namesi
Name:Ak1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:87977. Ak1.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • outer dense fiber Source: MGI
  • plasma membrane Source: MGI
  • sperm flagellum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589111 – 194Adenylate kinase isoenzyme 1Add BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineUniRule annotationBy similarity1
Modified residuei38PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9R0Y5.
PeptideAtlasiQ9R0Y5.
PRIDEiQ9R0Y5.
TopDownProteomicsiQ9R0Y5-1. [Q9R0Y5-1]

2D gel databases

REPRODUCTION-2DPAGEIPI00128209.
Q9R0Y5.

PTM databases

iPTMnetiQ9R0Y5.
PhosphoSitePlusiQ9R0Y5.
SwissPalmiQ9R0Y5.

Expressioni

Developmental stagei

Up-regulated during late spermiogenesis, when the flagellum is being assembled.1 Publication

Gene expression databases

BgeeiENSMUSG00000026817.
CleanExiMM_AK1.
ExpressionAtlasiQ9R0Y5. baseline and differential.
GenevisibleiQ9R0Y5. MM.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi198045. 2 interactors.
IntActiQ9R0Y5. 4 interactors.
MINTiMINT-4130855.
STRINGi10090.ENSMUSP00000068479.

Structurei

3D structure databases

ProteinModelPortaliQ9R0Y5.
SMRiQ9R0Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 67NMPbindUniRule annotationAdd BLAST30
Regioni131 – 141LIDUniRule annotationAdd BLAST11

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOGENOMiHOG000238771.
HOVERGENiHBG108060.
InParanoidiQ9R0Y5.
KOiK00939.
OMAiPREKNQG.
OrthoDBiEOG091G0OQ7.
PhylomeDBiQ9R0Y5.
TreeFamiTF354283.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03171. Adenylate_kinase_AK1. 1 hit.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR028582. AK1.
IPR006267. AK1/5.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0Y5-1) [UniParc]FASTAAdd to basket
Also known as: Ak1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEKLKKAKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG
60 70 80 90 100
SERGKKLSAI MEKGELVPLD TVLDMLRDAM LAKVDSSNGF LIDGYPREVK
110 120 130 140 150
QGEEFEQKIG QPTLLLYVDA GAETMTQRLL KRGETSGRVD DNEETIKKRL
160 170 180 190
ETYYNATEPV ISFYDKRGIV RKVNAEGTVD TVFSEVCTYL DSLK
Length:194
Mass (Da):21,540
Last modified:May 1, 2000 - v1
Checksum:i88E7862522967D14
GO
Isoform 2 (identifier: Q9R0Y5-2) [UniParc]FASTAAdd to basket
Also known as: Ak1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: ME → MGCCVSSEPQEEGGRKTG

Show »
Length:210
Mass (Da):23,116
Checksum:i5295189A6E7CB6D8
GO

Sequence cautioni

The sequence CAM16612 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0248431 – 2ME → MGCCVSSEPQEEGGRKTG in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010108 mRNA. Translation: CAB52407.1.
AJ010109 mRNA. Translation: CAB52408.1.
DQ486026 mRNA. Translation: ABF46940.1.
AK046613 mRNA. Translation: BAC32808.1.
AK089270 mRNA. Translation: BAC40822.1.
AL772271 Genomic DNA. Translation: CAM16612.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16613.1.
AL772271 Genomic DNA. Translation: CAM16614.1.
BC014802 mRNA. Translation: AAH14802.1.
BC054366 mRNA. Translation: AAH54366.1.
CCDSiCCDS15924.1. [Q9R0Y5-2]
CCDS57167.1. [Q9R0Y5-1]
RefSeqiNP_001185719.1. NM_001198790.1. [Q9R0Y5-1]
NP_001185720.1. NM_001198791.1. [Q9R0Y5-1]
NP_001185721.1. NM_001198792.1. [Q9R0Y5-1]
NP_067490.1. NM_021515.3. [Q9R0Y5-2]
XP_006497687.1. XM_006497624.3. [Q9R0Y5-1]
UniGeneiMm.29189.

Genome annotation databases

EnsembliENSMUST00000068271; ENSMUSP00000068479; ENSMUSG00000026817. [Q9R0Y5-2]
ENSMUST00000113277; ENSMUSP00000108902; ENSMUSG00000026817. [Q9R0Y5-1]
ENSMUST00000113278; ENSMUSP00000108903; ENSMUSG00000026817. [Q9R0Y5-1]
GeneIDi11636.
KEGGimmu:11636.
UCSCiuc008jgh.2. mouse. [Q9R0Y5-1]
uc008jgj.2. mouse. [Q9R0Y5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010108 mRNA. Translation: CAB52407.1.
AJ010109 mRNA. Translation: CAB52408.1.
DQ486026 mRNA. Translation: ABF46940.1.
AK046613 mRNA. Translation: BAC32808.1.
AK089270 mRNA. Translation: BAC40822.1.
AL772271 Genomic DNA. Translation: CAM16612.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16613.1.
AL772271 Genomic DNA. Translation: CAM16614.1.
BC014802 mRNA. Translation: AAH14802.1.
BC054366 mRNA. Translation: AAH54366.1.
CCDSiCCDS15924.1. [Q9R0Y5-2]
CCDS57167.1. [Q9R0Y5-1]
RefSeqiNP_001185719.1. NM_001198790.1. [Q9R0Y5-1]
NP_001185720.1. NM_001198791.1. [Q9R0Y5-1]
NP_001185721.1. NM_001198792.1. [Q9R0Y5-1]
NP_067490.1. NM_021515.3. [Q9R0Y5-2]
XP_006497687.1. XM_006497624.3. [Q9R0Y5-1]
UniGeneiMm.29189.

3D structure databases

ProteinModelPortaliQ9R0Y5.
SMRiQ9R0Y5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198045. 2 interactors.
IntActiQ9R0Y5. 4 interactors.
MINTiMINT-4130855.
STRINGi10090.ENSMUSP00000068479.

PTM databases

iPTMnetiQ9R0Y5.
PhosphoSitePlusiQ9R0Y5.
SwissPalmiQ9R0Y5.

2D gel databases

REPRODUCTION-2DPAGEIPI00128209.
Q9R0Y5.

Proteomic databases

PaxDbiQ9R0Y5.
PeptideAtlasiQ9R0Y5.
PRIDEiQ9R0Y5.
TopDownProteomicsiQ9R0Y5-1. [Q9R0Y5-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068271; ENSMUSP00000068479; ENSMUSG00000026817. [Q9R0Y5-2]
ENSMUST00000113277; ENSMUSP00000108902; ENSMUSG00000026817. [Q9R0Y5-1]
ENSMUST00000113278; ENSMUSP00000108903; ENSMUSG00000026817. [Q9R0Y5-1]
GeneIDi11636.
KEGGimmu:11636.
UCSCiuc008jgh.2. mouse. [Q9R0Y5-1]
uc008jgj.2. mouse. [Q9R0Y5-2]

Organism-specific databases

CTDi203.
MGIiMGI:87977. Ak1.

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOGENOMiHOG000238771.
HOVERGENiHBG108060.
InParanoidiQ9R0Y5.
KOiK00939.
OMAiPREKNQG.
OrthoDBiEOG091G0OQ7.
PhylomeDBiQ9R0Y5.
TreeFamiTF354283.

Enzyme and pathway databases

BRENDAi2.7.4.3. 3474.
ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

PROiQ9R0Y5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026817.
CleanExiMM_AK1.
ExpressionAtlasiQ9R0Y5. baseline and differential.
GenevisibleiQ9R0Y5. MM.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03171. Adenylate_kinase_AK1. 1 hit.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR028582. AK1.
IPR006267. AK1/5.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD1_MOUSE
AccessioniPrimary (citable) accession number: Q9R0Y5
Secondary accession number(s): A2AK80, Q542C5, Q9R0Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.