Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adenylate kinase isoenzyme 1

Gene

Ak1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity). May provide a mechanism to buffer the adenylate energy charge for sperm motility.By similarity1 Publication

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39AMPUniRule annotation1
Binding sitei44AMPUniRule annotation1
Binding sitei101AMPUniRule annotation1
Binding sitei132ATPUniRule annotation1
Binding sitei138AMPUniRule annotation1
Binding sitei149AMPUniRule annotation1
Binding sitei177ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 23ATPUniRule annotation6
Nucleotide bindingi65 – 67AMPUniRule annotation3
Nucleotide bindingi94 – 97AMPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.3 3474
ReactomeiR-MMU-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 1UniRule annotation (EC:2.7.4.3UniRule annotation, EC:2.7.4.6UniRule annotation)
Short name:
AK 1UniRule annotation
Alternative name(s):
ATP-AMP transphosphorylase 1UniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
MyokinaseUniRule annotation
Gene namesi
Name:Ak1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:87977 Ak1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589111 – 194Adenylate kinase isoenzyme 1Add BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineUniRule annotationBy similarity1
Modified residuei38PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9R0Y5
PeptideAtlasiQ9R0Y5
PRIDEiQ9R0Y5
TopDownProteomicsiQ9R0Y5-1 [Q9R0Y5-1]

2D gel databases

REPRODUCTION-2DPAGEiIPI00128209
Q9R0Y5

PTM databases

iPTMnetiQ9R0Y5
PhosphoSitePlusiQ9R0Y5
SwissPalmiQ9R0Y5

Expressioni

Developmental stagei

Up-regulated during late spermiogenesis, when the flagellum is being assembled.1 Publication

Gene expression databases

BgeeiENSMUSG00000026817
CleanExiMM_AK1
ExpressionAtlasiQ9R0Y5 baseline and differential
GenevisibleiQ9R0Y5 MM

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi1980454 interactors.
IntActiQ9R0Y5 4 interactors.
MINTiQ9R0Y5
STRINGi10090.ENSMUSP00000068479

Structurei

3D structure databases

ProteinModelPortaliQ9R0Y5
SMRiQ9R0Y5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 67NMPbindUniRule annotationAdd BLAST30
Regioni131 – 141LIDUniRule annotationAdd BLAST11

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3079 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00390000016215
HOGENOMiHOG000238771
HOVERGENiHBG108060
InParanoidiQ9R0Y5
KOiK00939
OMAiYPREKNQ
OrthoDBiEOG091G0OQ7
PhylomeDBiQ9R0Y5
TreeFamiTF354283

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03171 Adenylate_kinase_AK1, 1 hit
InterProiView protein in InterPro
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR028582 AK1
IPR006267 AK1/5
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01360 aden_kin_iso1, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0Y5-1) [UniParc]FASTAAdd to basket
Also known as: Ak1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEKLKKAKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG
60 70 80 90 100
SERGKKLSAI MEKGELVPLD TVLDMLRDAM LAKVDSSNGF LIDGYPREVK
110 120 130 140 150
QGEEFEQKIG QPTLLLYVDA GAETMTQRLL KRGETSGRVD DNEETIKKRL
160 170 180 190
ETYYNATEPV ISFYDKRGIV RKVNAEGTVD TVFSEVCTYL DSLK
Length:194
Mass (Da):21,540
Last modified:May 1, 2000 - v1
Checksum:i88E7862522967D14
GO
Isoform 2 (identifier: Q9R0Y5-2) [UniParc]FASTAAdd to basket
Also known as: Ak1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: ME → MGCCVSSEPQEEGGRKTG

Show »
Length:210
Mass (Da):23,116
Checksum:i5295189A6E7CB6D8
GO

Sequence cautioni

The sequence CAM16612 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0248431 – 2ME → MGCCVSSEPQEEGGRKTG in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010108 mRNA Translation: CAB52407.1
AJ010109 mRNA Translation: CAB52408.1
DQ486026 mRNA Translation: ABF46940.1
AK046613 mRNA Translation: BAC32808.1
AK089270 mRNA Translation: BAC40822.1
AL772271 Genomic DNA Translation: CAM16612.1 Sequence problems.
AL772271 Genomic DNA Translation: CAM16613.1
AL772271 Genomic DNA Translation: CAM16614.1
BC014802 mRNA Translation: AAH14802.1
BC054366 mRNA Translation: AAH54366.1
CCDSiCCDS15924.1 [Q9R0Y5-2]
CCDS57167.1 [Q9R0Y5-1]
RefSeqiNP_001185719.1, NM_001198790.1 [Q9R0Y5-1]
NP_001185720.1, NM_001198791.1 [Q9R0Y5-1]
NP_001185721.1, NM_001198792.1 [Q9R0Y5-1]
NP_067490.1, NM_021515.3 [Q9R0Y5-2]
XP_006497687.1, XM_006497624.3 [Q9R0Y5-1]
UniGeneiMm.29189

Genome annotation databases

EnsembliENSMUST00000068271; ENSMUSP00000068479; ENSMUSG00000026817 [Q9R0Y5-2]
ENSMUST00000113277; ENSMUSP00000108902; ENSMUSG00000026817 [Q9R0Y5-1]
ENSMUST00000113278; ENSMUSP00000108903; ENSMUSG00000026817 [Q9R0Y5-1]
GeneIDi11636
KEGGimmu:11636
UCSCiuc008jgh.2 mouse [Q9R0Y5-1]
uc008jgj.2 mouse [Q9R0Y5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKAD1_MOUSE
AccessioniPrimary (citable) accession number: Q9R0Y5
Secondary accession number(s): A2AK80, Q542C5, Q9R0Y4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 1, 2000
Last modified: February 28, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome