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Protein

Solute carrier family 22 member 2

Gene

Slc22a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates tubular uptake of organic compounds from circulation. Mediates the influx of agmatine, serotonin, choline, ranitidine, histamin, creatinine, amantadine, memantine, acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP and amiloride (By similarity). Mediates the influx of adrenaline, noradrenaline (norepinephrine), dopamine, cimetidine, famotidine, metformin, N-1-methylnicotinamide (NMN), 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), oxaliplatin and cisplatin. Cisplatin may develop a nephrotoxic action. Transport of creatinine is inhibited by fluoroquinolones such as DX-619 and LVFX. This transporter is a major determinant of the anticancer activity of oxaliplatin and may contribute to antitumor specificity.By similarity6 Publications

Kineticsi

  1. KM=68.8 µM for cimetidine (at pH 7.4 and 37 degrees Celsius)2 Publications
  2. KM=60.6 µM for famotidine2 Publications
  3. KM=0.63 mM for metformin2 Publications
  4. KM=4.4 mM for noradrenaline2 Publications
  5. KM=1.3 mM for histamine2 Publications
  6. KM=1.9 mM for dopamine2 Publications
  7. KM=3.6 mM for serotonine2 Publications
  1. Vmax=1490 pmol/min/mg enzyme for cimetidine uptake2 Publications
  2. Vmax=117 pmol/min/mg enzyme for famotidine uptake2 Publications
  3. Vmax=1.446 nmol/min/mg enzyme for metformin uptake2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei451 – 4511Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cations

GO - Molecular functioni

  • choline transmembrane transporter activity Source: RGD
  • dopamine transmembrane transporter activity Source: GO_Central
  • organic cation transmembrane transporter activity Source: RGD
  • quaternary ammonium group transmembrane transporter activity Source: RGD
  • steroid binding Source: RGD

GO - Biological processi

  • dopamine transport Source: GO_Central
  • histamine transport Source: RGD
  • organic cation transport Source: RGD
  • quaternary ammonium group transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi2.A.1.19.19. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 2
Alternative name(s):
Organic cation transporter 2
Short name:
rOCT2
Gene namesi
Name:Slc22a2
Synonyms:Oct2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61936. Slc22a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence analysisAdd
BLAST
Transmembranei22 – 4221HelicalSequence analysisAdd
BLAST
Topological domaini43 – 150108ExtracellularSequence analysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence analysisAdd
BLAST
Topological domaini172 – 1776CytoplasmicSequence analysis
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Topological domaini199 – 21012ExtracellularSequence analysisAdd
BLAST
Transmembranei211 – 23121HelicalSequence analysisAdd
BLAST
Topological domaini232 – 2387CytoplasmicSequence analysis
Transmembranei239 – 25921HelicalSequence analysisAdd
BLAST
Topological domaini260 – 2634ExtracellularSequence analysis
Transmembranei264 – 28421HelicalSequence analysisAdd
BLAST
Topological domaini285 – 34864CytoplasmicSequence analysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence analysisAdd
BLAST
Topological domaini370 – 3756ExtracellularSequence analysis
Transmembranei376 – 39621HelicalSequence analysisAdd
BLAST
Topological domaini397 – 4048CytoplasmicSequence analysis
Transmembranei405 – 42521HelicalSequence analysisAdd
BLAST
Topological domaini426 – 4327ExtracellularSequence analysis
Transmembranei433 – 45321HelicalSequence analysisAdd
BLAST
Topological domaini454 – 46411CytoplasmicSequence analysisAdd
BLAST
Transmembranei465 – 48521HelicalSequence analysisAdd
BLAST
Topological domaini486 – 4949ExtracellularSequence analysis
Transmembranei495 – 51521HelicalSequence analysisAdd
BLAST
Topological domaini516 – 55540CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • integral component of plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi451 – 4511C → M: Transport activity strongly reduced. 1 Publication

Chemistry

ChEMBLiCHEMBL1770032.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Solute carrier family 22 member 2PRO_0000320962Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9R0W2.
PRIDEiQ9R0W2.

PTM databases

iPTMnetiQ9R0W2.
PhosphoSiteiQ9R0W2.

Expressioni

Tissue specificityi

Expressed in the kidney; in the proximal tubule of the outer medulla. Expression is greater in the kidney of male than of female.4 Publications

Inductioni

Down-regulated in ischemia/reperfusion (I/R) kidneys. Up-regulated by testosterone and moderately down-regulated by estradiol.2 Publications

Gene expression databases

BgeeiENSRNOG00000016625.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023116.

Chemistry

BindingDBiQ9R0W2.

Structurei

3D structure databases

ProteinModelPortaliQ9R0W2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
HOGENOMiHOG000234568.
HOVERGENiHBG061545.
InParanoidiQ9R0W2.
KOiK08199.
PhylomeDBiQ9R0W2.
TreeFamiTF315847.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0W2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVDDILEH IGEFHLFQKQ TFFLLALLSG AFTPIYVGIV FLGFTPDHHC
60 70 80 90 100
WSPGAAKLSQ RCGWSQAEEL NYTVPGLGPS DEASFLSQCM RYEVDWNQST
110 120 130 140 150
LDCVDPLSSL AADRNQLPLG PCEHGWVYNT PGSSIVTEFN LVCAHSWMLD
160 170 180 190 200
LFQSVVNVGF FIGAMMIGYL ADRFGRKFCL LVTILINAIS GALMAISPNY
210 220 230 240 250
AWMLVFRFLQ GLVSKAGWLI GYILITEFVG LGYRRMVGIC YQIAFTVGLL
260 270 280 290 300
ILAGVAYVIP NWRWLQFAVT LPNFCFLLYF WCIPESPRWL ISQNKIVKAM
310 320 330 340 350
KIIKHIAKKN GKSVPVSLQN LTPDEDAGKK LNPSFLDLVR TPQIRKHTLI
360 370 380 390 400
LMYNWFTSSV LYQGLIMHMG LAGDNIYLDF FYSALVEFPA AFIIILTIDR
410 420 430 440 450
VGRRYPWAVS NMVAGAACLA SVFIPDDLQW LKITIACLGR MGITMAYEMV
460 470 480 490 500
CLVNAELYPT YIRNLGVLVC SSMCDIGGII TPFLVYRLTD IWMEFPLVVF
510 520 530 540 550
AVVGLVAGAL VLLLPETKGK ALPETIEDAE NMQRPRKKKE KRIYLQVKQA

DRPLS
Length:555
Mass (Da):62,343
Last modified:May 1, 2000 - v1
Checksum:i29521969AE1AC206
GO

Sequence cautioni

The sequence BAA11754 differs from that shown. Reason: Frameshift at position 539. Curated
The sequence CAA66979 differs from that shown. Reason: Frameshift at position 539. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti332 – 3321N → K in BAA11754 (PubMed:8702418).Curated
Sequence conflicti335 – 3351F → I in BAA11754 (PubMed:8702418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83044 mRNA. Translation: BAA11754.1. Frameshift.
X98334 mRNA. Translation: CAA66979.1. Frameshift.
Y13154 mRNA. Translation: CAB52215.1.
PIRiJC4884.
RefSeqiNP_113772.1. NM_031584.2.
UniGeneiRn.10555.

Genome annotation databases

GeneIDi29503.
KEGGirno:29503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83044 mRNA. Translation: BAA11754.1. Frameshift.
X98334 mRNA. Translation: CAA66979.1. Frameshift.
Y13154 mRNA. Translation: CAB52215.1.
PIRiJC4884.
RefSeqiNP_113772.1. NM_031584.2.
UniGeneiRn.10555.

3D structure databases

ProteinModelPortaliQ9R0W2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023116.

Chemistry

BindingDBiQ9R0W2.
ChEMBLiCHEMBL1770032.

Protein family/group databases

TCDBi2.A.1.19.19. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ9R0W2.
PhosphoSiteiQ9R0W2.

Proteomic databases

PaxDbiQ9R0W2.
PRIDEiQ9R0W2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29503.
KEGGirno:29503.

Organism-specific databases

CTDi6582.
RGDi61936. Slc22a2.

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
HOGENOMiHOG000234568.
HOVERGENiHBG061545.
InParanoidiQ9R0W2.
KOiK08199.
PhylomeDBiQ9R0W2.
TreeFamiTF315847.

Miscellaneous databases

PROiQ9R0W2.

Gene expression databases

BgeeiENSRNOG00000016625.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS22A2_RAT
AccessioniPrimary (citable) accession number: Q9R0W2
Secondary accession number(s): P70485, P97558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.