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Protein

Serine/arginine-rich splicing factor 10

Gene

Srsf10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (By similarity). May be involved in regulation of alternative splicing in neurons (PubMed:10583508).By similarity1 Publication

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

  • cytosolic transport Source: UniProtKB
  • mRNA splice site selection Source: UniProtKB
  • mRNA splicing, via spliceosome Source: MGI
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of mRNA splicing, via spliceosome Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB
  • RNA splicing, via transesterification reactions Source: UniProtKB

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 10
Alternative name(s):
FUS-interacting serine-arginine-rich protein 1
Neural-salient serine/arginine-rich protein
Neural-specific SR protein
Splicing factor, arginine/serine-rich 13A
TLS-associated protein with Ser-Arg repeats
Short name:
TASR
Short name:
TLS-associated protein with SR repeats
TLS-associated serine-arginine protein
Short name:
TLS-associated SR protein
Gene namesi
Name:Srsf10
Synonyms:Fusip1, Nssr, Sfrs13a, Srsf13a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1333805 Srsf10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815941 – 262Serine/arginine-rich splicing factor 10Add BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei108PhosphoserineBy similarity1
Modified residuei129PhosphoserineBy similarity1
Modified residuei131PhosphoserineBy similarity1
Modified residuei133PhosphoserineCombined sources1
Modified residuei158PhosphoserineBy similarity1
Modified residuei160PhosphoserineBy similarity1
Isoform 3 (identifier: Q9R0U0-3)
Modified residuei158PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. Fully dephosphorylated in mitosis and partially dephosphorylated on heat shock.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9R0U0
PRIDEiQ9R0U0

PTM databases

iPTMnetiQ9R0U0
PhosphoSitePlusiQ9R0U0

Expressioni

Tissue specificityi

Widely expressed, with high levels in brain and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000028676
CleanExiMM_FUSIP1
ExpressionAtlasiQ9R0U0 baseline and differential
GenevisibleiQ9R0U0 MM

Interactioni

Subunit structurei

The phosphorylated but not the dephosphorylated form interacts with TRA2B/SFRS10. The dephosphorylated form interacts with SNRNP70. Interacts with FUS C-terminus. Interacts with YTHDC1, leading to inhibit RNA-binding activity of SRSF10.By similarity

GO - Molecular functioni

  • unfolded protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi199597, 3 interactors
IntActiQ9R0U0, 1 interactor
MINTiQ9R0U0
STRINGi10090.ENSMUSP00000101479

Structurei

3D structure databases

ProteinModelPortaliQ9R0U0
SMRiQ9R0U0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 88RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi106 – 260Arg/Ser-rich (RS domain)Add BLAST155

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Phylogenomic databases

eggNOGiKOG0118 Eukaryota
COG0724 LUCA
GeneTreeiENSGT00700000104403
HOVERGENiHBG107480
InParanoidiQ9R0U0
KOiK12900
OMAiFAYVQYT
OrthoDBiEOG091G13K0
TreeFamiTF351864

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034476 SRSF10
PANTHERiPTHR23147:SF5 PTHR23147:SF5, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0U0-1) [UniParc]FASTAAdd to basket
Also known as: TASR-1, NSSR1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRYLRPPNT SLFVRNVADD TRSEDLRREF GRYGPIVDVY VPLDFYTRRP
60 70 80 90 100
RGFAYVQFED VRDAEDALHN LDRKWICGRQ IEIQFAQGDR KTPNQMKAKE
110 120 130 140 150
GRNVYSSSRY DDYDRYRRSR SRSYERRRSR SRSFDYNYRR SYSPRNSRPT
160 170 180 190 200
GRPRRSRSHS DNDRFKHRNR SFSRSKSNSR SRSKSQPKKE MKAKSRSRSA
210 220 230 240 250
SHTKTRGTSK TDSKTHYKSG SRYEKESRKK EPPRSKSQSR SQSRSRSKSR
260
SRSWTSPKSS GH
Length:262
Mass (Da):31,301
Last modified:May 24, 2004 - v2
Checksum:i205F95D36CBBFAB4
GO
Isoform 2 (identifier: Q9R0U0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: Missing.

Show »
Length:261
Mass (Da):31,213
Checksum:iA07499B11D4C7570
GO
Isoform 3 (identifier: Q9R0U0-3) [UniParc]FASTAAdd to basket
Also known as: TASR-2, NSSR2

The sequence of this isoform differs from the canonical sequence as follows:
     165-183: FKHRNRSFSRSKSNSRSRS → PNCSWNTQYSSAYYTSRKI
     184-262: Missing.

Show »
Length:183
Mass (Da):22,222
Checksum:i4AA87CAA9B51A131
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22R → Q in AAH37591 (PubMed:15489334).Curated1
Sequence conflicti154R → T in BAA35092 (PubMed:10583508).Curated1
Sequence conflicti154R → T in BAA35093 (PubMed:10583508).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010426147Missing in isoform 2. 1 Publication1
Alternative sequenceiVSP_010427165 – 183FKHRN…SRSRS → PNCSWNTQYSSAYYTSRKI in isoform 3. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_010428184 – 262Missing in isoform 3. 2 PublicationsAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042383 mRNA Translation: AAC70916.1
AB015894 mRNA Translation: BAA35092.1
AB015895 mRNA Translation: BAA35093.1
AF060490 mRNA Translation: AAC26715.1
AK014345 mRNA Translation: BAB29286.1
AK168524 mRNA Translation: BAE40403.1
AK168558 mRNA Translation: BAE40431.1
AL672076 Genomic DNA No translation available.
BC037591 mRNA Translation: AAH37591.1
BC043060 mRNA Translation: AAH43060.1
BC083082 mRNA Translation: AAH83082.1
CCDSiCCDS18791.1 [Q9R0U0-3]
CCDS38922.1 [Q9R0U0-1]
CCDS71493.1 [Q9R0U0-2]
RefSeqiNP_001073856.1, NM_001080387.2 [Q9R0U0-1]
NP_001271124.1, NM_001284195.1 [Q9R0U0-2]
NP_001271125.1, NM_001284196.1
NP_034308.1, NM_010178.3 [Q9R0U0-3]
UniGeneiMm.10229
Mm.472035

Genome annotation databases

EnsembliENSMUST00000097844; ENSMUSP00000095455; ENSMUSG00000028676 [Q9R0U0-2]
ENSMUST00000105853; ENSMUSP00000101479; ENSMUSG00000028676 [Q9R0U0-1]
ENSMUST00000126641; ENSMUSP00000114564; ENSMUSG00000028676 [Q9R0U0-3]
GeneIDi14105
KEGGimmu:14105
UCSCiuc008vhd.2 mouse [Q9R0U0-2]
uc033ifn.1 mouse [Q9R0U0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSRS10_MOUSE
AccessioniPrimary (citable) accession number: Q9R0U0
Secondary accession number(s): B1AV44
, B1AV45, O70307, O88468, Q3TGW7, Q8CFZ1, Q9R0T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: May 23, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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