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Q9R0T8 (IKKE_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Short name=I-kappa-B kinase epsilon
Short name=IKK-E
Short name=IKK-epsilon
Short name=IkBKE
EC=2.7.11.10
Alternative name(s):
Inducible I kappa-B kinase
Short name=IKK-i
Gene names
Name:Ikbke
Synonyms:Ikke, Ikki
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length717 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death By similarity.

Catalytic activity

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Subunit structure

May interact with MAVS/IPS1. Interacts with AZI2. Interacts with SIKE1. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between IKBKE and SIKE1. Interacts with TOPORS; induced by DNA damage. Interacts with CYLD By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Nucleus › PML body By similarity. Note: Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent By similarity.

Induction

By lipopolysaccharide (LPS).

Post-translational modification

Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1 By similarity.

Autophosphorylated and phosphorylated by IKBKB/IKKB By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 717717Inhibitor of nuclear factor kappa-B kinase subunit epsilon
PRO_0000086018

Regions

Domain9 – 315307Protein kinase
Nucleotide binding15 – 239ATP By similarity
Region452 – 47322Leucine-zipper

Sites

Active site1351Proton acceptor By similarity
Binding site381ATP By similarity

Amino acid modifications

Modified residue1721Phosphoserine; by IKKB By similarity
Modified residue6651Phosphoserine By similarity
Cross-link231Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) By similarity

Experimental info

Sequence conflict4711S → G in BAA85154. Ref.1
Sequence conflict4831A → S in BAA85154. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9R0T8 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 0863ECC180AE385A

FASTA71780,953
        10         20         30         40         50         60 
MQSTTNYLWH TDDLLGQGAT ASVYKARNKK SGEVVAVKVF NSASYRRPPE VQVREFEVLR 

        70         80         90        100        110        120 
RLNHQNIVKL FAVEETGGSR QKVLIMEYCS SGSLLSVLED PENTFGLSEE EFLVVLRCVV 

       130        140        150        160        170        180 
AGMNHLRENG IVHRDIKPGN IMRLVGEEGQ SIYKLSDFGA ARKLDDDEKF VSVYGTEEYL 

       190        200        210        220        230        240 
HPDMYERAVL RKPQQKAFGV TVDLWSIGVT LYHAATGSLP FIPFGGPRRN KEIMYRITTE 

       250        260        270        280        290        300 
KPAGAISGTQ KQENGPLEWS YSLPITCRLS MGLQNQLVPI LANILEVEED KCWGFDQFFA 

       310        320        330        340        350        360 
ETSDILQRTV IHVFSLPQAV LHHVYIHAHN TIAIFLEAVY EQTNVTPKHQ EYLFEGHPCV 

       370        380        390        400        410        420 
LEPSLSAQHI AHTAASSPLT LFSMSSDTPK GLAFRDPALD VPKFVPKVDL QADYSTAKGV 

       430        440        450        460        470        480 
LGAGYQALWL ARVLLDGQAL MLRGLHWVLE VLQDTCQQTL EVTRTALLYL SSSLGTERFS 

       490        500        510        520        530        540 
SGAGMPDVQE RKEATELRTR LQTLSEILSK CSHNVTETQR SLSCLGEELL KNRDQIHEDN 

       550        560        570        580        590        600 
KSIQKIQCCL DKMHFIYKQF KKSRMRPGLS YNEEQIHKLD KVNFSHLAKR LLQVFQEECV 

       610        620        630        640        650        660 
QTYQVSLVTH GKRMRQVQRA QNHLHLIGHS VATCNSEARG AQESLNKIFD QLLLDRASEQ 

       670        680        690        700        710 
GAEVSPQPMA PHPGPDPKDL VFHMQELCND MKLLAFDLQD NNRLIERLHR VPSAPDV

« Hide

References

« Hide 'large scale' references
[1]"IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases."
Shimada T., Kawai T., Takeda K., Matsumoto M., Inoue J., Tatsumi Y., Kanamaru A., Akira S.
Int. Immunol. 11:1357-1362(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Macrophage.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Bone marrow and Thymus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB016589 mRNA. Translation: BAA85154.1.
AK088580 mRNA. Translation: BAC40434.1.
AK149911 mRNA. Translation: BAE29161.1.
CT010206 mRNA. Translation: CAJ18414.1.
CH466520 Genomic DNA. Translation: EDL39710.1.
IPIIPI00128110.
RefSeqNP_062751.2. NM_019777.3.
UniGeneMm.386783.

3D structure databases

ProteinModelPortalQ9R0T8.
SMRQ9R0T8. Positions 2-460.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000054126.

PTM databases

PhosphoSiteQ9R0T8.

Proteomic databases

PRIDEQ9R0T8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000062108; ENSMUSP00000054126; ENSMUSG00000042349.
GeneID56489.
KEGGmmu:56489.

Organism-specific databases

CTD9641.
MGIMGI:1929612. Ikbke.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000063051.
HOGENOMHOG000220867.
HOVERGENHBG008494.
InParanoidQ8C2I3.
KOK07211.
OMAHIYIHAH.
OrthoDBEOG4S4PFW.

Enzyme and pathway databases

BRENDA2.7.11.10. 3474.

Gene expression databases

ArrayExpressQ9R0T8.
BgeeQ9R0T8.
CleanExMM_IKBKE.
GenevestigatorQ9R0T8.
GermOnlineENSMUSG00000042349. Mus musculus.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio312766.
SOURCESearch...

Entry information

Entry nameIKKE_MOUSE
AccessionPrimary (citable) accession number: Q9R0T8
Secondary accession number(s): Q8C2I3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: July 27, 2011
Last modified: May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents