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Q9R0T4

- CADH1_RAT

UniProt

Q9R0T4 - CADH1_RAT

Protein

Cadherin-1

Gene

Cdh1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 By similarity.By similarity
    E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi261 – 2611Calcium 1By similarity
    Metal bindingi261 – 2611Calcium 2By similarity
    Metal bindingi292 – 2921Calcium 3By similarity
    Sitei704 – 7052Cleavage; by a metalloproteinaseBy similarity
    Sitei735 – 7362Cleavage; by gamma-secretase/PS1By similarity
    Sitei754 – 7552Cleavage; by caspase-3By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: Ensembl
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to amino acid stimulus Source: Ensembl
    2. cellular response to indole-3-methanol Source: Ensembl
    3. cellular response to lithium ion Source: Ensembl
    4. cochlea development Source: Ensembl
    5. epithelial cell morphogenesis Source: Ensembl
    6. homophilic cell adhesion Source: InterPro
    7. intestinal epithelial cell development Source: Ensembl
    8. negative regulation of canonical Wnt signaling pathway Source: Ensembl
    9. negative regulation of cell-cell adhesion Source: Ensembl
    10. negative regulation of epithelial cell proliferation Source: Ensembl
    11. neuron projection development Source: RGD
    12. pituitary gland development Source: RGD
    13. positive regulation of transcription, DNA-templated Source: Ensembl
    14. positive regulation of transcription factor import into nucleus Source: Ensembl
    15. protein homooligomerization Source: Ensembl
    16. protein localization to plasma membrane Source: Ensembl
    17. protein metabolic process Source: Ensembl
    18. regulation of branching involved in salivary gland morphogenesis Source: Ensembl
    19. regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    20. regulation of protein localization to cell surface Source: Ensembl
    21. regulation of water loss via skin Source: Ensembl
    22. response to drug Source: RGD
    23. response to organic substance Source: RGD
    24. response to toxic substance Source: RGD
    25. salivary gland cavitation Source: Ensembl
    26. sensory perception of sound Source: Ensembl
    27. single organismal cell-cell adhesion Source: DFLAT
    28. synapse assembly Source: RGD
    29. tight junction assembly Source: Ensembl
    30. trophectodermal cell differentiation Source: Ensembl

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_199178. Degradation of the extracellular matrix.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cadherin-1
    Alternative name(s):
    Epithelial cadherin
    Short name:
    E-cadherin
    Uvomorulin
    CD_antigen: CD324
    Cleaved into the following 3 chains:
    Gene namesi
    Name:Cdh1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi69279. Cdh1.

    Subcellular locationi

    Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome By similarity. Golgi apparatustrans-Golgi network By similarity
    Note: Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. aggresome Source: Ensembl
    3. apical junction complex Source: Ensembl
    4. apical part of cell Source: Ensembl
    5. axon terminus Source: Ensembl
    6. basolateral plasma membrane Source: Ensembl
    7. catenin complex Source: Ensembl
    8. cell-cell adherens junction Source: Ensembl
    9. cell surface Source: Ensembl
    10. cytoplasm Source: RGD
    11. cytoplasmic side of plasma membrane Source: Ensembl
    12. endosome Source: UniProtKB-SubCell
    13. focal adhesion Source: Ensembl
    14. integral component of membrane Source: UniProtKB-KW
    15. lateral loop Source: Ensembl
    16. lateral plasma membrane Source: Ensembl
    17. node of Ranvier Source: Ensembl
    18. perinuclear region of cytoplasm Source: Ensembl
    19. plasma membrane Source: RGD
    20. Schmidt-Lanterman incisure Source: Ensembl
    21. trans-Golgi network Source: Ensembl

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Propeptidei24 – 158135Sequence AnalysisPRO_0000003719Add
    BLAST
    Chaini159 – 886728Cadherin-1PRO_0000003720Add
    BLAST
    Chaini705 – 886182E-Cad/CTF1Sequence AnalysisPRO_0000236073Add
    BLAST
    Chaini736 – 886151E-Cad/CTF2Sequence AnalysisPRO_0000236074Add
    BLAST
    Chaini755 – 886132E-Cad/CTF3Sequence AnalysisPRO_0000236075Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi562 – 5621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi641 – 6411N-linked (GlcNAc...)Sequence Analysis
    Modified residuei757 – 7571Phosphotyrosine; by SRCBy similarity
    Modified residuei758 – 7581Phosphotyrosine; by SRCBy similarity
    Modified residuei759 – 7591Phosphotyrosine; by SRCBy similarity
    Modified residuei842 – 8421PhosphoserineBy similarity
    Modified residuei844 – 8441PhosphoserineBy similarity
    Modified residuei850 – 8501PhosphoserineBy similarity

    Post-translational modificationi

    N-glycosylation at Asn-641 is essential for expression, folding and trafficking.By similarity
    Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-758 By similarity.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ9R0T4.
    PRIDEiQ9R0T4.

    PTM databases

    PhosphoSiteiQ9R0T4.

    Miscellaneous databases

    PMAP-CutDBQ9R0T4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9R0T4.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1, beta-catenin/CTNNB1 or gamma-catenin/JUP, and potentially alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5 By similarity. Interacts with TBC1D2. Interacts with LIMA1. Interacts with CAV1. Interacts with the TRPV4 and CTNNB1 complex By similarity. Interacts with PIP5K1C. Interacts with DDR1; this stabilizes CDH1 at the cell surface and inhibits its internalization By similarity. Interacts with RAPGEF2 By similarity. Interacts with RAB8B.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi249721. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0T4.
    SMRiQ9R0T4. Positions 159-376, 788-881.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 713690ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini735 – 886152CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei714 – 73421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini159 – 266108Cadherin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini267 – 379113Cadherin 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini380 – 490111Cadherin 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini491 – 597107Cadherin 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini598 – 701104Cadherin 5PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni762 – 77312Required for binding CTNND1 and PSEN1By similarityAdd
    BLAST
    Regioni815 – 88672Required for binding alpha, beta andBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi842 – 85716Ser-richAdd
    BLAST

    Domaini

    Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.By similarity

    Sequence similaritiesi

    Contains 5 cadherin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG328838.
    GeneTreeiENSGT00740000115112.
    HOGENOMiHOG000231254.
    HOVERGENiHBG106438.
    InParanoidiQ9R0T4.
    KOiK05689.
    OMAiDFGVGQE.
    OrthoDBiEOG7PS1DS.
    PhylomeDBiQ9R0T4.
    TreeFamiTF316817.

    Family and domain databases

    Gene3Di2.60.40.60. 6 hits.
    4.10.900.10. 1 hit.
    InterProiIPR002126. Cadherin.
    IPR015919. Cadherin-like.
    IPR020894. Cadherin_CS.
    IPR000233. Cadherin_cytoplasmic-dom.
    IPR014868. Cadherin_pro_dom.
    IPR027397. Catenin_binding_dom.
    [Graphical view]
    PfamiPF00028. Cadherin. 5 hits.
    PF01049. Cadherin_C. 1 hit.
    PF08758. Cadherin_pro. 1 hit.
    [Graphical view]
    PRINTSiPR00205. CADHERIN.
    SMARTiSM00112. CA. 4 hits.
    SM01055. Cadherin_pro. 1 hit.
    [Graphical view]
    SUPFAMiSSF49313. SSF49313. 6 hits.
    PROSITEiPS00232. CADHERIN_1. 3 hits.
    PS50268. CADHERIN_2. 5 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9R0T4-1 [UniParc]FASTAAdd to Basket

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    MGARCRSFSA LLLLLQVSSW LCQQPESESD SCRPGFSSEV YTFLVPERHL    50
    ERGHILGRVK FEGCTGRPRT AFFSEDSRFK VSTDGVITVK RHLKLHKLET 100
    SFLVHAWDSS YRKLSTKVTL KSLGHHHHRH HHRDPVSESN PELLTFPSFH 150
    QGLRRQKRDW VIPPINCPEN QKGEFPQRLV QIKSNRDKET TVFYSITGPG 200
    ADKPPVGVFI IERETGWLKV TQPLDREAID KYLLYSHAVS SNGEAVEDPM 250
    EIVVTVTDQN DNRPEFIQEV FEGSVAEGAL PGTSVMQVSA TDADDDINTY 300
    NAAIAYTILS QDPELPHKNM FTVNRDTGVI SVVTSGLDRE SYPTYTLVVQ 350
    AADLQGEGLS TTAKAVITVK DINDNAPIFN PSTYQGQVLE NEVGARIATL 400
    KVTDDDAPNT PAWNAVYTVV NDPDHQFTVI TDPKTNEGIL KTAKGLDFEA 450
    KQQYILHVTV ENEEPFEGSL VPSTATVTVD VVDVNEAPIF VPAEKRVEVP 500
    EDFGVGLEIA SYTAREPDTF MEQKITYRIW RDTANWLEIN PETGVISTRA 550
    EMDREDSEHV KNSTYTALII ATDDGSPIAT GTGTLLLVLS DVNDNAPIPE 600
    PRNMQFCQRN PKPHVITILD PDLPPNTSPF TAELTHGASV NWTIEYNDAE 650
    QESLILQPRK DLEIGEYKIN LKLSDNQNKD QVTTLEVHVC DCEGTVNNCM 700
    KAISLEAGLQ VPAILGILGG ILALLILILL LLLFLRRRTV VKEPLLPPDD 750
    DTRDNVYYYD EEGGGEEDQD FDLSQLHRGL DARPEVIRND VAPTLMSMPQ 800
    YRPRPANPDE IGNFIDENLK AADSDPTAPP YDSLLVFDYE GSGSEAASLS 850
    SLNSSESDQD QDYDYLNEWG NRFKKLADMY GGGEED 886
    Length:886
    Mass (Da):98,715
    Last modified:May 1, 2000 - v1
    Checksum:iA9AEE28EB797A547
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017696 mRNA. Translation: BAA84920.1.
    AF177680 mRNA. Translation: AAF87055.1.
    AJ000540 mRNA. Translation: CAA04173.1.
    RefSeqiNP_112624.1. NM_031334.1.
    UniGeneiRn.1303.

    Genome annotation databases

    EnsembliENSRNOT00000027346; ENSRNOP00000027346; ENSRNOG00000020151.
    GeneIDi83502.
    KEGGirno:83502.
    UCSCiRGD:69279. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017696 mRNA. Translation: BAA84920.1 .
    AF177680 mRNA. Translation: AAF87055.1 .
    AJ000540 mRNA. Translation: CAA04173.1 .
    RefSeqi NP_112624.1. NM_031334.1.
    UniGenei Rn.1303.

    3D structure databases

    ProteinModelPortali Q9R0T4.
    SMRi Q9R0T4. Positions 159-376, 788-881.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249721. 3 interactions.

    PTM databases

    PhosphoSitei Q9R0T4.

    Proteomic databases

    PaxDbi Q9R0T4.
    PRIDEi Q9R0T4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027346 ; ENSRNOP00000027346 ; ENSRNOG00000020151 .
    GeneIDi 83502.
    KEGGi rno:83502.
    UCSCi RGD:69279. rat.

    Organism-specific databases

    CTDi 999.
    RGDi 69279. Cdh1.

    Phylogenomic databases

    eggNOGi NOG328838.
    GeneTreei ENSGT00740000115112.
    HOGENOMi HOG000231254.
    HOVERGENi HBG106438.
    InParanoidi Q9R0T4.
    KOi K05689.
    OMAi DFGVGQE.
    OrthoDBi EOG7PS1DS.
    PhylomeDBi Q9R0T4.
    TreeFami TF316817.

    Enzyme and pathway databases

    Reactomei REACT_199178. Degradation of the extracellular matrix.

    Miscellaneous databases

    NextBioi 615920.
    PMAP-CutDB Q9R0T4.
    PROi Q9R0T4.

    Gene expression databases

    Genevestigatori Q9R0T4.

    Family and domain databases

    Gene3Di 2.60.40.60. 6 hits.
    4.10.900.10. 1 hit.
    InterProi IPR002126. Cadherin.
    IPR015919. Cadherin-like.
    IPR020894. Cadherin_CS.
    IPR000233. Cadherin_cytoplasmic-dom.
    IPR014868. Cadherin_pro_dom.
    IPR027397. Catenin_binding_dom.
    [Graphical view ]
    Pfami PF00028. Cadherin. 5 hits.
    PF01049. Cadherin_C. 1 hit.
    PF08758. Cadherin_pro. 1 hit.
    [Graphical view ]
    PRINTSi PR00205. CADHERIN.
    SMARTi SM00112. CA. 4 hits.
    SM01055. Cadherin_pro. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49313. SSF49313. 6 hits.
    PROSITEi PS00232. CADHERIN_1. 3 hits.
    PS50268. CADHERIN_2. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Asai K., Tada T., Yamamoto M., Obayashi M., Mizuno M., Toda A., Eimoto T., Kato T.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Jejunum.
    2. "Multiple cadherin superfamily members with unique expression profiles are produced in rat testis."
      Johnson K.J., Patel S.R., Boekelheide K.
      Endocrinology 141:675-683(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 769-872.
      Strain: Fischer 344.
      Tissue: Testis.
    3. Gibbons K.L.
      Thesis (1997), University of Technology / Sydney, Australia
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 779-846.
      Tissue: Mammary tumor.
    4. "Rab8B GTPase and junction dynamics in the testis."
      Lau A.S., Mruk D.D.
      Endocrinology 144:1549-1563(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAB8B.

    Entry informationi

    Entry nameiCADH1_RAT
    AccessioniPrimary (citable) accession number: Q9R0T4
    Secondary accession number(s): O35794, Q9JIV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3