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Protein

DnaJ homolog subfamily C member 3

Gene

Dnajc3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUnfolded protein response

Enzyme and pathway databases

ReactomeiR-RNO-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-RNO-6798695 Neutrophil degranulation
R-RNO-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 3
Alternative name(s):
Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
Protein kinase inhibitor of 58 kDa
Short name:
Protein kinase inhibitor p58
Gene namesi
Name:Dnajc3
Synonyms:P58ipk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi708518 Dnajc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000007104732 – 504DnaJ homolog subfamily C member 3Add BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi248 ↔ 258By similarity
Modified residuei274PhosphoserineBy similarity1
Disulfide bondi313 ↔ 329By similarity
Modified residuei475PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9R0T3
PRIDEiQ9R0T3

PTM databases

iPTMnetiQ9R0T3
PhosphoSitePlusiQ9R0T3

Expressioni

Gene expression databases

BgeeiENSRNOG00000010352
GenevisibleiQ9R0T3 RN

Interactioni

Subunit structurei

Interacts with EIF2AK2 and EIF2AK3. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with THAP12 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9R0T3, 1 interactor
STRINGi10116.ENSRNOP00000014182

Structurei

3D structure databases

ProteinModelPortaliQ9R0T3
SMRiQ9R0T3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 70TPR 1Add BLAST34
Repeati72 – 104TPR 2Add BLAST33
Repeati105 – 138TPR 3Add BLAST34
Repeati154 – 187TPR 4Add BLAST34
Repeati188 – 221TPR 5Add BLAST34
Repeati222 – 255TPR 6Add BLAST34
Repeati268 – 301TPR 7Add BLAST34
Repeati306 – 339TPR 8Add BLAST34
Repeati340 – 373TPR 9Add BLAST34
Domaini394 – 462JPROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni375 – 393Flexible linkerBy similarityAdd BLAST19

Domaini

The J domain mediates interaction with HSPA8.
Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.By similarity

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG0624 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00890000139418
HOGENOMiHOG000193351
HOVERGENiHBG053820
InParanoidiQ9R0T3
KOiK09523
OMAiGAECGVN
OrthoDBiEOG091G0783
PhylomeDBiQ9R0T3
TreeFamiTF105162

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF13181 TPR_8, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SM00028 TPR, 7 hits
SUPFAMiSSF46565 SSF46565, 1 hit
SSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit
PS50005 TPR, 8 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0T3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAPGSVRSR LGAVFPFLLV LVDLQYEGAE CGVNADVEKH LELGKKLLAA
60 70 80 90 100
GQLADALSQF HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTRVIEL
110 120 130 140 150
KMDFTAARLQ RGHLLLKQGR LAEAEDDFKK VLKSNPSENE EKEAQSQLVK
160 170 180 190 200
ADEMQRLRAQ ALDAFDSADY TAAITFLDEI LEVCVWDAEL RELRAECFIK
210 220 230 240 250
EGEPRKAISD LKAASKLKND NTEAFYKISI LYYQLGDHEL SLSEVRECLK
260 270 280 290 300
LDQDHKRCFA HYKQVKKLNK LIGSAEELIR DGRYTDATSK YESVMKAEPS
310 320 330 340 350
VAEYTVRSKE RICHCFSKDE KPVEAIKICS EVLQLEPDNV NALKDRAEAY
360 370 380 390 400
LIEEMYDEAI QDYEAAQEQN ENDQQIREGL EKAQRLLKQS QKRDYYKILG
410 420 430 440 450
VKRNAKKQEI IKAYRKLALQ WHPDNFQSEE EKKKAEKKFI DIAAAKEVLS
460 470 480 490 500
DPEMRRKFDD GEDPLDAETQ QGGGSNPFHR SWDSWQGFNP FSSGGPFRFK

FHFN
Length:504
Mass (Da):57,561
Last modified:August 16, 2005 - v3
Checksum:i1268B6A7771163DD
GO

Sequence cautioni

The sequence AAH61764 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312I → V in BAA86882 (Ref. 1) Curated1
Sequence conflicti347A → T in BAA86882 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017702 mRNA Translation: BAA86882.3
BC061764 mRNA Translation: AAH61764.1 Sequence problems.
RefSeqiNP_071568.1, NM_022232.1
UniGeneiRn.162234

Genome annotation databases

EnsembliENSRNOT00000014182; ENSRNOP00000014182; ENSRNOG00000010352
GeneIDi63880
KEGGirno:63880
UCSCiRGD:708518 rat

Similar proteinsi

Entry informationi

Entry nameiDNJC3_RAT
AccessioniPrimary (citable) accession number: Q9R0T3
Secondary accession number(s): Q6P7A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: May 23, 2018
This is version 128 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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