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Q9R0R1

- TRFM_MOUSE

UniProt

Q9R0R1 - TRFM_MOUSE

Protein

Melanotransferrin

Gene

Mfi2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi78 – 781Iron 1PROSITE-ProRule annotation
    Metal bindingi107 – 1071Iron 1PROSITE-ProRule annotation
    Binding sitei132 – 1321Carbonate 1PROSITE-ProRule annotation
    Binding sitei136 – 1361Carbonate 1PROSITE-ProRule annotation
    Binding sitei138 – 1381Carbonate 1; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei139 – 1391Carbonate 1; via amide nitrogenPROSITE-ProRule annotation
    Metal bindingi210 – 2101Iron 1PROSITE-ProRule annotation
    Metal bindingi279 – 2791Iron 1PROSITE-ProRule annotation
    Metal bindingi451 – 4511Iron 2PROSITE-ProRule annotation
    Metal bindingi556 – 5561Iron 2PROSITE-ProRule annotation
    Metal bindingi625 – 6251Iron 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro

    GO - Biological processi

    1. iron ion homeostasis Source: UniProtKB-KW
    2. iron ion transport Source: InterPro

    Keywords - Biological processi

    Ion transport, Iron transport, Transport

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiS60.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Melanotransferrin
    Alternative name(s):
    Membrane-bound transferrin-like protein p97
    Short name:
    MTf
    CD_antigen: CD228
    Gene namesi
    Name:Mfi2
    Synonyms:Mtf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1353421. Mfi2.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: InterPro
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919By similarityAdd
    BLAST
    Chaini20 – 709690MelanotransferrinPRO_0000035741Add
    BLAST
    Propeptidei710 – 73829Removed in mature formSequence AnalysisPRO_0000035742Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 63PROSITE-ProRule annotation
    Disulfide bondi36 ↔ 54PROSITE-ProRule annotation
    Glycosylationi118 – 1181N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi130 ↔ 216PROSITE-ProRule annotation
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi172 ↔ 189PROSITE-ProRule annotation
    Disulfide bondi186 ↔ 199PROSITE-ProRule annotation
    Disulfide bondi257 ↔ 271PROSITE-ProRule annotation
    Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
    Lipidationi709 – 7091GPI-anchor amidated cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9R0R1.
    PRIDEiQ9R0R1.

    PTM databases

    PhosphoSiteiQ9R0R1.

    Expressioni

    Gene expression databases

    BgeeiQ9R0R1.
    GenevestigatoriQ9R0R1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0R1.
    SMRiQ9R0R1. Positions 23-710.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 357335Transferrin-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini366 – 706341Transferrin-like 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the transferrin family.PROSITE-ProRule annotation
    Contains 2 transferrin-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG87503.
    GeneTreeiENSGT00390000001619.
    HOGENOMiHOG000043759.
    HOVERGENiHBG000055.
    InParanoidiQ9R0R1.
    KOiK06569.
    OMAiTAVSEFF.
    OrthoDBiEOG7D59N7.
    PhylomeDBiQ9R0R1.
    TreeFamiTF324013.

    Family and domain databases

    InterProiIPR016357. Transferrin.
    IPR001156. Transferrin-like_dom.
    IPR018195. Transferrin_Fe_BS.
    [Graphical view]
    PfamiPF00405. Transferrin. 2 hits.
    [Graphical view]
    PIRSFiPIRSF002549. Transferrin. 1 hit.
    PRINTSiPR00422. TRANSFERRIN.
    SMARTiSM00094. TR_FER. 2 hits.
    [Graphical view]
    PROSITEiPS00205. TRANSFERRIN_LIKE_1. 2 hits.
    PS00206. TRANSFERRIN_LIKE_2. 2 hits.
    PS00207. TRANSFERRIN_LIKE_3. 2 hits.
    PS51408. TRANSFERRIN_LIKE_4. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9R0R1-1 [UniParc]FASTAAdd to Basket

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    MRLLSVTFWL LLSLRTVVCV MEVQWCTISD AEQQKCKDMS EAFQGAGIRP    50
    SLLCVQGNSA DHCVQLIKEQ KADAITLDGG AIYEAGKEHG LKPVVGEVYD 100
    QDIGTSYYAV AVVRRNSNVT INTLKGVKSC HTGINRTVGW NVPVGYLVES 150
    GHLSVMGCDV LKAVGDYFGG SCVPGTGETS HSESLCRLCR GDSSGHNVCD 200
    KSPLERYYDY SGAFRCLAEG AGDVAFVKHS TVLENTDGNT LPSWGKSLMS 250
    EDFQLLCRDG SRADITEWRR CHLAKVPAHA VVVRGDMDGG LIFQLLNEGQ 300
    LLFSHEDSSF QMFSSKAYSQ KNLLFKDSTL ELVPIATQNY EAWLGQEYLQ 350
    AMKGLLCDPN RLPHYLRWCV LSAPEIQKCG DMAVAFSRQN LKPEIQCVSA 400
    ESPEHCMEQI QAGHTDAVTL RGEDIYRAGK VYGLVPAAGE LYAEEDRSNS 450
    YFVVAVARRD SSYSFTLDEL RGKRSCHPYL GSPAGWEVPI GSLIQRGFIR 500
    PKDCDVLTAV SQFFNASCVP VNNPKNYPSA LCALCVGDEK GRNKCVGSSQ 550
    ERYYGYSGAF RCLVEHAGDV AFVKHTTVFE NTNGHNPEPW ASHLRWQDYE 600
    LLCPNGARAE VDQFQACNLA QMPSHAVMVR PDTNIFTVYG LLDKAQDLFG 650
    DDHNKNGFQM FDSSKYHSQD LLFKDATVRA VPVREKTTYL DWLGPDYVVA 700
    LEGMLSQQCS GAGAAVQRVP LLALLLLTLA AGLLPRVL 738
    Length:738
    Mass (Da):81,293
    Last modified:May 1, 2000 - v1
    Checksum:iB5D9BFAB30F4B8B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024336 mRNA. Translation: BAA86655.1.
    AB047799 Genomic DNA. Translation: BAB41139.1.
    BC040347 mRNA. Translation: AAH40347.1.
    CCDSiCCDS28108.1.
    RefSeqiNP_038928.1. NM_013900.2.
    UniGeneiMm.30988.

    Genome annotation databases

    EnsembliENSMUST00000023464; ENSMUSP00000023464; ENSMUSG00000022780.
    GeneIDi30060.
    KEGGimmu:30060.
    UCSCiuc007yxv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024336 mRNA. Translation: BAA86655.1 .
    AB047799 Genomic DNA. Translation: BAB41139.1 .
    BC040347 mRNA. Translation: AAH40347.1 .
    CCDSi CCDS28108.1.
    RefSeqi NP_038928.1. NM_013900.2.
    UniGenei Mm.30988.

    3D structure databases

    ProteinModelPortali Q9R0R1.
    SMRi Q9R0R1. Positions 23-710.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S60.973.

    PTM databases

    PhosphoSitei Q9R0R1.

    Proteomic databases

    PaxDbi Q9R0R1.
    PRIDEi Q9R0R1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023464 ; ENSMUSP00000023464 ; ENSMUSG00000022780 .
    GeneIDi 30060.
    KEGGi mmu:30060.
    UCSCi uc007yxv.1. mouse.

    Organism-specific databases

    CTDi 4241.
    MGIi MGI:1353421. Mfi2.

    Phylogenomic databases

    eggNOGi NOG87503.
    GeneTreei ENSGT00390000001619.
    HOGENOMi HOG000043759.
    HOVERGENi HBG000055.
    InParanoidi Q9R0R1.
    KOi K06569.
    OMAi TAVSEFF.
    OrthoDBi EOG7D59N7.
    PhylomeDBi Q9R0R1.
    TreeFami TF324013.

    Miscellaneous databases

    NextBioi 307198.
    PROi Q9R0R1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9R0R1.
    Genevestigatori Q9R0R1.

    Family and domain databases

    InterProi IPR016357. Transferrin.
    IPR001156. Transferrin-like_dom.
    IPR018195. Transferrin_Fe_BS.
    [Graphical view ]
    Pfami PF00405. Transferrin. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF002549. Transferrin. 1 hit.
    PRINTSi PR00422. TRANSFERRIN.
    SMARTi SM00094. TR_FER. 2 hits.
    [Graphical view ]
    PROSITEi PS00205. TRANSFERRIN_LIKE_1. 2 hits.
    PS00206. TRANSFERRIN_LIKE_2. 2 hits.
    PS00207. TRANSFERRIN_LIKE_3. 2 hits.
    PS51408. TRANSFERRIN_LIKE_4. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Membrane-bound transferrin-like protein (MTf): structure, evolution and selective expression during chondrogenic differentiation of mouse embryonic cells."
      Nakamasu K., Kawamoto T., Shen M., Gotoh O., Teramoto M., Noshiro M., Kato Y.
      Biochim. Biophys. Acta 1447:258-264(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure and promoter analysis of the mouse membrane-bound transferrin-like protein (MTf) gene."
      Nakamasu K., Kawamoto T., Yoshida E., Noshiro M., Matsuda Y., Kato Y.
      Eur. J. Biochem. 268:1468-1476(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiTRFM_MOUSE
    AccessioniPrimary (citable) accession number: Q9R0R1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 6, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3