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Q9R0R1 (TRFM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Melanotransferrin
Alternative name(s):
Membrane-bound transferrin-like protein p97
Short name=MTf
CD_antigen=CD228
Gene names
Name:Mfi2
Synonyms:Mtf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length738 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor By similarity.

Sequence similarities

Belongs to the transferrin family.

Contains 2 transferrin-like domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Chain20 – 709690Melanotransferrin
PRO_0000035741
Propeptide710 – 73829Removed in mature form Potential
PRO_0000035742

Regions

Domain23 – 357335Transferrin-like 1
Domain366 – 706341Transferrin-like 2

Sites

Metal binding781Iron 1 By similarity
Metal binding1071Iron 1 By similarity
Metal binding2101Iron 1 By similarity
Metal binding2791Iron 1 By similarity
Metal binding4511Iron 2 By similarity
Metal binding5561Iron 2 By similarity
Metal binding6251Iron 2 By similarity
Binding site1321Carbonate 1 By similarity
Binding site1361Carbonate 1 By similarity
Binding site1381Carbonate 1; via amide nitrogen By similarity
Binding site1391Carbonate 1; via amide nitrogen By similarity

Amino acid modifications

Lipidation7091GPI-anchor amidated cysteine Potential
Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation5151N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 63 By similarity
Disulfide bond36 ↔ 54 By similarity
Disulfide bond130 ↔ 216 By similarity
Disulfide bond172 ↔ 189 By similarity
Disulfide bond186 ↔ 199 By similarity
Disulfide bond257 ↔ 271 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9R0R1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: B5D9BFAB30F4B8B1

FASTA73881,293
        10         20         30         40         50         60 
MRLLSVTFWL LLSLRTVVCV MEVQWCTISD AEQQKCKDMS EAFQGAGIRP SLLCVQGNSA 

        70         80         90        100        110        120 
DHCVQLIKEQ KADAITLDGG AIYEAGKEHG LKPVVGEVYD QDIGTSYYAV AVVRRNSNVT 

       130        140        150        160        170        180 
INTLKGVKSC HTGINRTVGW NVPVGYLVES GHLSVMGCDV LKAVGDYFGG SCVPGTGETS 

       190        200        210        220        230        240 
HSESLCRLCR GDSSGHNVCD KSPLERYYDY SGAFRCLAEG AGDVAFVKHS TVLENTDGNT 

       250        260        270        280        290        300 
LPSWGKSLMS EDFQLLCRDG SRADITEWRR CHLAKVPAHA VVVRGDMDGG LIFQLLNEGQ 

       310        320        330        340        350        360 
LLFSHEDSSF QMFSSKAYSQ KNLLFKDSTL ELVPIATQNY EAWLGQEYLQ AMKGLLCDPN 

       370        380        390        400        410        420 
RLPHYLRWCV LSAPEIQKCG DMAVAFSRQN LKPEIQCVSA ESPEHCMEQI QAGHTDAVTL 

       430        440        450        460        470        480 
RGEDIYRAGK VYGLVPAAGE LYAEEDRSNS YFVVAVARRD SSYSFTLDEL RGKRSCHPYL 

       490        500        510        520        530        540 
GSPAGWEVPI GSLIQRGFIR PKDCDVLTAV SQFFNASCVP VNNPKNYPSA LCALCVGDEK 

       550        560        570        580        590        600 
GRNKCVGSSQ ERYYGYSGAF RCLVEHAGDV AFVKHTTVFE NTNGHNPEPW ASHLRWQDYE 

       610        620        630        640        650        660 
LLCPNGARAE VDQFQACNLA QMPSHAVMVR PDTNIFTVYG LLDKAQDLFG DDHNKNGFQM 

       670        680        690        700        710        720 
FDSSKYHSQD LLFKDATVRA VPVREKTTYL DWLGPDYVVA LEGMLSQQCS GAGAAVQRVP 

       730 
LLALLLLTLA AGLLPRVL

« Hide

References

« Hide 'large scale' references
[1]"Membrane-bound transferrin-like protein (MTf): structure, evolution and selective expression during chondrogenic differentiation of mouse embryonic cells."
Nakamasu K., Kawamoto T., Shen M., Gotoh O., Teramoto M., Noshiro M., Kato Y.
Biochim. Biophys. Acta 1447:258-264(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure and promoter analysis of the mouse membrane-bound transferrin-like protein (MTf) gene."
Nakamasu K., Kawamoto T., Yoshida E., Noshiro M., Matsuda Y., Kato Y.
Eur. J. Biochem. 268:1468-1476(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB024336 mRNA. Translation: BAA86655.1.
AB047799 Genomic DNA. Translation: BAB41139.1.
BC040347 mRNA. Translation: AAH40347.1.
IPIIPI00128030.
RefSeqNP_038928.1. NM_013900.2.
UniGeneMm.30988.

3D structure databases

ProteinModelPortalQ9R0R1.
SMRQ9R0R1. Positions 22-710.
ModBaseSearch...

Protein family/group databases

MEROPSS60.973.

PTM databases

PhosphoSiteQ9R0R1.

Proteomic databases

PaxDbQ9R0R1.
PRIDEQ9R0R1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023464; ENSMUSP00000023464; ENSMUSG00000022780.
GeneID30060.
KEGGmmu:30060.

Organism-specific databases

CTD4241.
MGIMGI:1353421. Mfi2.

Phylogenomic databases

eggNOGNOG87503.
GeneTreeENSGT00390000001619.
HOGENOMHOG000043759.
HOVERGENHBG000055.
InParanoidQ9R0R1.
KOK06569.
OMADGGLIFR.
OrthoDBEOG4G4GPZ.

Gene expression databases

ArrayExpressQ9R0R1.
BgeeQ9R0R1.
GenevestigatorQ9R0R1.
GermOnlineENSMUSG00000022780. Mus musculus.

Family and domain databases

InterProIPR016357. Transferrin.
IPR001156. Transferrin_fam.
IPR018195. Transferrin_Fe_BS.
[Graphical view]
PANTHERPTHR11485. PTHR11485. 1 hit.
PfamPF00405. Transferrin. 2 hits.
[Graphical view]
PIRSFPIRSF002549. Transferrin. 1 hit.
PRINTSPR00422. TRANSFERRIN.
SMARTSM00094. TR_FER. 2 hits.
[Graphical view]
PROSITEPS00205. TRANSFERRIN_LIKE_1. 2 hits.
PS00206. TRANSFERRIN_LIKE_2. 2 hits.
PS00207. TRANSFERRIN_LIKE_3. 2 hits.
PS51408. TRANSFERRIN_LIKE_4. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio307198.
SOURCESearch...

Entry information

Entry nameTRFM_MOUSE
AccessionPrimary (citable) accession number: Q9R0R1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: May 1, 2000
Last modified: May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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