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Protein

C-type lectin domain family 4 member E

Gene

Clec4e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.3 Publications

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • signaling receptor activity Source: UniProtKB

GO - Biological processi

  • defense response to bacterium Source: MGI
  • Fc-gamma receptor signaling pathway Source: MGI
  • immune response Source: UniProtKB
  • innate immune response Source: MGI
  • positive regulation of cytokine secretion Source: UniProtKB
  • T cell differentiation involved in immune response Source: MGI

Keywordsi

Biological processImmunity
LigandLectin

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 4 member E
Alternative name(s):
C-type lectin superfamily member 9
Macrophage-inducible C-type lectin
Gene namesi
Name:Clec4e
Synonyms:Clecsf9, Mincle
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1861232 Clec4e

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 45Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini46 – 214ExtracellularSequence analysisAdd BLAST169

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Macrophages from deficient mice shown impaired cytokine/chemokine production, and the mutant mice have diminished inflammatory responses. In vivo inflammatory response against Malassezia is impaired in mutant mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42R → I: Abolishes the association with FCER1G. 1 Publication1
Mutagenesisi169E → Q: Abrogates Malassezia recognition; when associated with D-171. 1 Publication1
Mutagenesisi171N → D: Abrogates Malassezia recognition; when associated with Q-169. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000466201 – 214C-type lectin domain family 4 member EAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi80 ↔ 91PROSITE-ProRule annotation
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi108 ↔ 205PROSITE-ProRule annotation
Disulfide bondi179 ↔ 197PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9R0Q8
PaxDbiQ9R0Q8
PRIDEiQ9R0Q8

PTM databases

PhosphoSitePlusiQ9R0Q8
SwissPalmiQ9R0Q8

Expressioni

Tissue specificityi

Expressions were observed in peritoneal macrophage, macrophage cell line RAW 264.7, and myeloblastic leukemia cell line M1 following inflammatory stimuli.1 Publication

Inductioni

Expression is strongly induced by LPS and several proinflammatory cytokines, including IFN-gamma and TNF.1 Publication

Gene expression databases

BgeeiENSMUSG00000030142
CleanExiMM_CLEC4E
ExpressionAtlasiQ9R0Q8 baseline and differential
GenevisibleiQ9R0Q8 MM

Interactioni

Subunit structurei

Monomer. Homodimer.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032239

Structurei

3D structure databases

ProteinModelPortaliQ9R0Q8
SMRiQ9R0Q8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 206C-type lectinPROSITE-ProRule annotationAdd BLAST120

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297 Eukaryota
ENOG410XPJ1 LUCA
GeneTreeiENSGT00760000118924
HOGENOMiHOG000059556
HOVERGENiHBG050992
InParanoidiQ9R0Q8
KOiK10059
OMAiDCATIRD
OrthoDBiEOG091G0DW5
PhylomeDBiQ9R0Q8
TreeFamiTF333341

Family and domain databases

CDDicd03590 CLECT_DC-SIGN_like, 1 hit
Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR018378 C-type_lectin_CS
IPR033989 CD209-like_CTLD
IPR016187 CTDL_fold
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS00615 C_TYPE_LECTIN_1, 1 hit
PS50041 C_TYPE_LECTIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9R0Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTKSPASH HTERGCFKNS QVLSWTIAGA SILFLSGCFI TRCVVTYRSS
60 70 80 90 100
QISGQNLQPH RNIKELSCYS EASGSVKNCC PLNWKHYQSS CYFFSTTTLT
110 120 130 140 150
WSSSLKNCSD MGAHLVVIDT QEEQEFLFRT KPKRKEFYIG LTDQVVEGQW
160 170 180 190 200
QWVDDTPFTE SLSFWDAGEP NNIVLVEDCA TIRDSSNSRK NWNDIPCFYS
210
MPWICEMPEI SPLD
Length:214
Mass (Da):24,431
Last modified:May 1, 2000 - v1
Checksum:i2FEF318A69BDAFE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024717 mRNA Translation: BAA83754.1
BC003218 mRNA Translation: AAH03218.1
CCDSiCCDS20514.1
RefSeqiNP_064332.1, NM_019948.2
UniGeneiMm.248327

Genome annotation databases

EnsembliENSMUST00000032239; ENSMUSP00000032239; ENSMUSG00000030142
GeneIDi56619
KEGGimmu:56619
UCSCiuc009dqk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCLC4E_MOUSE
AccessioniPrimary (citable) accession number: Q9R0Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health