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Protein

Transmembrane emp24 domain-containing protein 2

Gene

Tmed2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing (By similarity). Required for morphogenesis of embryo and placenta.By similarity1 Publication

GO - Biological processi

  • embryonic morphogenesis Source: UniProtKB
  • embryonic placenta development Source: MGI
  • Golgi organization Source: UniProtKB
  • heart looping Source: MGI
  • intracellular protein transport Source: MGI
  • in utero embryonic development Source: MGI
  • labyrinthine layer blood vessel development Source: CACAO
  • maternal placenta development Source: UniProtKB
  • multicellular organism growth Source: MGI
  • negative regulation of GTPase activity Source: UniProtKB
  • neural tube closure Source: MGI
  • positive regulation of gene expression Source: MGI
  • post-anal tail morphogenesis Source: MGI
  • protein targeting to plasma membrane Source: UniProtKB
  • somite rostral/caudal axis specification Source: MGI
  • somitogenesis Source: MGI
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 2
Alternative name(s):
COPI-coated vesicle membrane protein p24
Membrane protein p24A
Sid 394
p24 family protein beta-1
Short name:
p24beta1
Gene namesi
Name:Tmed2
Synonyms:Rnp24, Sid394
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1929269. Tmed2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 168148LumenalSequence analysisAdd
BLAST
Transmembranei169 – 18921HelicalSequence analysisAdd
BLAST
Topological domaini190 – 20112CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi15 – 151L → E: Embryonic lethal. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 201181Transmembrane emp24 domain-containing protein 2PRO_0000010382Add
BLAST

Proteomic databases

EPDiQ9R0Q3.
MaxQBiQ9R0Q3.
PaxDbiQ9R0Q3.
PeptideAtlasiQ9R0Q3.
PRIDEiQ9R0Q3.

PTM databases

iPTMnetiQ9R0Q3.
PhosphoSiteiQ9R0Q3.

Expressioni

Developmental stagei

Expressed during embryonal and placental development.1 Publication

Gene expression databases

BgeeiQ9R0Q3.
CleanExiMM_TMED2.
ExpressionAtlasiQ9R0Q3. baseline and differential.
GenevisibleiQ9R0Q3. MM.

Interactioni

Subunit structurei

Monomer and homodimer in the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and Golgi. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED7, TMED9 and TMED10. Interacts with TMED10. Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED2. Interacts with SEC23A; indicative for an association of TMED2 with the COPII vesicle coat. Interacts with ARF1 and ARFGAP1. Interacts with CD59, SEC24A, SEC24B, SEC24C, SEC24D and ATLA1. Interacts with KDELR1; the interaction is decreased by KDEL ligand. Interacts with F2RL1; the interaction occurs at the Golgi apparatus. Interacts with CASR (immaturely glycosylated form); the interaction occurs in the endoplasmic reticulum-Golgi intermediate compartment or cis-Golgi. Interacts with F2RL1; the interaction occurs at the Golgi apparatus. Interacts with GORASP1 and GORASP2. Found in a complex composed at least of SURF4, TMED2 and TMED10 (By similarity).By similarity

Protein-protein interaction databases

BioGridi207909. 116 interactions.
IntActiQ9R0Q3. 120 interactions.
MINTiMINT-1863429.
STRINGi10090.ENSMUSP00000054834.

Structurei

3D structure databases

ProteinModelPortaliQ9R0Q3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 11283GOLDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 181181Interaction with F2RL1By similarityAdd
BLAST
Regioni118 – 15740Required for TMED10 and TMED2 cis-Golgi network localizationBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili117 – 16751Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi194 – 2018COPI vesicle coat-bindingSequence analysis
Motifi194 – 1952COPII vesicle coat-bindingSequence analysis

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1692. Eukaryota.
ENOG410XQCK. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG105106.
InParanoidiQ9R0Q3.
OMAiFWIETPS.
OrthoDBiEOG7RFTJP.
PhylomeDBiQ9R0Q3.
TreeFamiTF313000.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTLAELLAL LAALLATASG YFVSIDAHAE ECFFERVTSG TKMGLIFEVA
60 70 80 90 100
EGGFLDIDVE ITGPDNKGIY KGDRESSGKY TFAAHMDGTY KFCFSNRMST
110 120 130 140 150
MTPKIVMFTI DIGEAPKGQD METEAHQNKL EEMINELAVA MTAVKHEQEY
160 170 180 190 200
MEVRERIHRA INDNTNSRVV LWSFFEALVL VAMTLGQIYY LKRFFEVRRV

V
Length:201
Mass (Da):22,705
Last modified:May 1, 2000 - v1
Checksum:iF5DE259FC419CF85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025218 mRNA. Translation: BAA84689.1.
BC083140 mRNA. Translation: AAH83140.1.
CCDSiCCDS19679.1.
RefSeqiNP_062744.1. NM_019770.2.
XP_003945495.1. XM_003945446.3.
UniGeneiMm.325307.
Mm.425279.

Genome annotation databases

EnsembliENSMUST00000060226; ENSMUSP00000054834; ENSMUSG00000029390.
GeneIDi100862175.
56334.
KEGGimmu:100862175.
mmu:56334.
UCSCiuc008zqa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025218 mRNA. Translation: BAA84689.1.
BC083140 mRNA. Translation: AAH83140.1.
CCDSiCCDS19679.1.
RefSeqiNP_062744.1. NM_019770.2.
XP_003945495.1. XM_003945446.3.
UniGeneiMm.325307.
Mm.425279.

3D structure databases

ProteinModelPortaliQ9R0Q3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207909. 116 interactions.
IntActiQ9R0Q3. 120 interactions.
MINTiMINT-1863429.
STRINGi10090.ENSMUSP00000054834.

PTM databases

iPTMnetiQ9R0Q3.
PhosphoSiteiQ9R0Q3.

Proteomic databases

EPDiQ9R0Q3.
MaxQBiQ9R0Q3.
PaxDbiQ9R0Q3.
PeptideAtlasiQ9R0Q3.
PRIDEiQ9R0Q3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060226; ENSMUSP00000054834; ENSMUSG00000029390.
GeneIDi100862175.
56334.
KEGGimmu:100862175.
mmu:56334.
UCSCiuc008zqa.1. mouse.

Organism-specific databases

CTDi10959.
MGIiMGI:1929269. Tmed2.

Phylogenomic databases

eggNOGiKOG1692. Eukaryota.
ENOG410XQCK. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG105106.
InParanoidiQ9R0Q3.
OMAiFWIETPS.
OrthoDBiEOG7RFTJP.
PhylomeDBiQ9R0Q3.
TreeFamiTF313000.

Enzyme and pathway databases

ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiQ9R0Q3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0Q3.
CleanExiMM_TMED2.
ExpressionAtlasiQ9R0Q3. baseline and differential.
GenevisibleiQ9R0Q3. MM.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse mRNA for transmembrane protein."
    Seki N., Hattori A., Hayashi A., Kozuma S., Muramatsu M., Saito T.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  4. "The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta."
    Jerome-Majewska L.A., Achkar T., Luo L., Lupu F., Lacy E.
    Dev. Biol. 341:154-166(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF LEU-15.

Entry informationi

Entry nameiTMED2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Embryonic lethal for homozygotes with Glu-15 in the signal peptide. Animals do not show Tmed2 protein expression and also have reduced protein levels of Tmed7 and Tmed10. Prior to death at mid-gestation, embryos exhibit developmental delay, abnormal rostral-caudal elongation, randomized heart looping, and absence of the labyrinth layer of the placenta.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.