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Protein

Synaptotagmin-like protein 4

Gene

Sytl4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 105FYVE-typeAdd BLAST43

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: GO_Central
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: GO_Central
  • neurexin family protein binding Source: UniProtKB
  • phospholipid binding Source: MGI
  • Rab GTPase binding Source: InterPro
  • syntaxin binding Source: GO_Central
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-like protein 4
Alternative name(s):
Exophilin-2
Granuphilin
Gene namesi
Name:Sytl4
Synonyms:Slp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1351606 Sytl4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14E → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi18I → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi21V → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi32D → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi43L → A: Strongly reduces interaction with STX1A. 1 Publication1
Mutagenesisi118W → S: Strongly reduces interaction with RAB27A. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902171 – 673Synaptotagmin-like protein 4Add BLAST673

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei202PhosphoserineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei218PhosphoserineCombined sources1
Modified residuei222PhosphoserineBy similarity1
Modified residuei275PhosphoserineCombined sources1
Modified residuei490PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9R0Q1
PRIDEiQ9R0Q1

PTM databases

iPTMnetiQ9R0Q1
PhosphoSitePlusiQ9R0Q1

Expressioni

Tissue specificityi

Detected in the pancreatic islet, in particular in insulin-positive beta cells, and in pituitary.1 Publication

Gene expression databases

BgeeiENSMUSG00000031255
CleanExiMM_SYTL4
ExpressionAtlasiQ9R0Q1 baseline and differential
GenevisibleiQ9R0Q1 MM

Interactioni

Subunit structurei

Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B. Interacts with MYO5A.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab27aQ9ERI22EBI-15734647,EBI-398172

GO - Molecular functioni

  • clathrin binding Source: GO_Central
  • neurexin family protein binding Source: UniProtKB
  • Rab GTPase binding Source: InterPro
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

DIPiDIP-31497N
IntActiQ9R0Q1, 4 interactors
STRINGi10090.ENSMUSP00000033608

Structurei

3D structure databases

ProteinModelPortaliQ9R0Q1
SMRiQ9R0Q1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 122RabBDPROSITE-ProRule annotationAdd BLAST119
Domaini360 – 464C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini515 – 619C2 2PROSITE-ProRule annotationAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi306 – 313Poly-Glu8

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 105FYVE-typeAdd BLAST43

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1028 Eukaryota
ENOG410XRME LUCA
GeneTreeiENSGT00810000125376
HOGENOMiHOG000231332
HOVERGENiHBG054255
InParanoidiQ9R0Q1
KOiK17598
OMAiHYNHTFV
OrthoDBiEOG091G07OO
PhylomeDBiQ9R0Q1
TreeFamiTF341184

Family and domain databases

CDDicd04029 C2A_SLP-4_5, 1 hit
Gene3Di2.60.40.150, 2 hits
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR010911 Rab_BD
IPR037303 SLP-4/5_C2A
IPR028694 SYTL4
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10024:SF9 PTHR10024:SF9, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
SMARTiView protein in SMART
SM00239 C2, 2 hits
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
PS50916 RABBD, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0Q1-1) [UniParc]FASTAAdd to basket
Also known as: Granuphilin-a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEILDLSFL SEMERDLILG VLQRDEELRK ADEKRIRRLK NELLEIKRKG
60 70 80 90 100
AKRGSQHYSD RTCARCQEGL GRLIPKSSTC VGCNHLVCRE CRVLESNGSW
110 120 130 140 150
RCKVCSKEIE LKKATGDWFY DQKVNRFDYR TGSEIIRMSL RQKPAVNKRE
160 170 180 190 200
TAGQSLLQQT QMGDIWPGRR IIQEQQQREQ SVLFEVPKTR SGKSALEAES
210 220 230 240 250
ESLDSYTADS DSTSRRDSLD KSGLFPEWKK MSAPKSQVEK EIPPGNQNAV
260 270 280 290 300
CGDEGDMVFK KNTKKVLRPS EYTKSVIDLR PEDVAQESGI LGDRSKSVPG
310 320 330 340 350
LSVDMEEEEE EEEDIDHLVK LHRQKLARGS MQSGSSMSTL GSIMSIYSEA
360 370 380 390 400
GDFGNISVTG KIAFSLKFEQ KTQTLVIHVK ECHQLAYADE AKKRSNPYVK
410 420 430 440 450
TYLLPDKSRQ GKRKTSIKRD TINPLYDETF RYEISESLLA QRTLQFSVWH
460 470 480 490 500
HGRFGRNTFL GEAEVHMDSW KLDKKLDHCL PLHGKISTES SPGLPAHKGE
510 520 530 540 550
LVVSLKYIPA SKLPVGGDRK KSKGGEGGEL QVWIKEAKNL TAAKSGGTSD
560 570 580 590 600
SFVKGYLLPM RNKASKRKTP VMKKTLSPHY NHTFVYNGVR LEDLQHMCLE
610 620 630 640 650
LTVWDREPLA SNDFLGGVRL GVGTGISNGE VVDWMDSTGE EVSLWQKMRQ
660 670
YPGSWAEGTL QLRSSMVKQK LGV
Length:673
Mass (Da):76,021
Last modified:May 1, 2000 - v1
Checksum:i2AC218E2A4BE4C8D
GO
Isoform 2 (identifier: Q9R0Q1-2) [UniParc]FASTAAdd to basket
Also known as: Granuphilin-b

The sequence of this isoform differs from the canonical sequence as follows:
     486-502: ISTESSPGLPAHKGELV → GSVMAKWWTGWIRLVKK
     503-673: Missing.

Show »
Length:502
Mass (Da):57,479
Checksum:i6353F899E39192A4
GO
Isoform 3 (identifier: Q9R0Q1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     486-673: ISTESSPGLP...SSMVKQKLGV → VVCFKGRSQLASQVNCL

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:502
Mass (Da):57,285
Checksum:i7EC60E5235FF9B0E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007902486 – 673ISTES…QKLGV → VVCFKGRSQLASQVNCL in isoform 3. 1 PublicationAdd BLAST188
Alternative sequenceiVSP_007900486 – 502ISTES…KGELV → GSVMAKWWTGWIRLVKK in isoform 2. 2 PublicationsAdd BLAST17
Alternative sequenceiVSP_007901503 – 673Missing in isoform 2. 2 PublicationsAdd BLAST171

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025258 mRNA Translation: BAA84656.1
AB025259 mRNA Translation: BAA84657.1
AK030401 mRNA Translation: BAC26945.1
AL691421 Genomic DNA No translation available.
BC026819 mRNA Translation: AAH26819.1
CCDSiCCDS30388.1 [Q9R0Q1-1]
CCDS72429.1 [Q9R0Q1-2]
CCDS81169.1 [Q9R0Q1-3]
RefSeqiNP_001277646.1, NM_001290717.1 [Q9R0Q1-2]
NP_001277647.1, NM_001290718.1 [Q9R0Q1-2]
NP_001277648.1, NM_001290719.1 [Q9R0Q1-3]
NP_038785.1, NM_013757.2 [Q9R0Q1-1]
XP_006528614.1, XM_006528551.2 [Q9R0Q1-1]
UniGeneiMm.38674

Genome annotation databases

EnsembliENSMUST00000033608; ENSMUSP00000033608; ENSMUSG00000031255 [Q9R0Q1-1]
ENSMUST00000113294; ENSMUSP00000108919; ENSMUSG00000031255 [Q9R0Q1-3]
ENSMUST00000113297; ENSMUSP00000108922; ENSMUSG00000031255 [Q9R0Q1-2]
GeneIDi27359
KEGGimmu:27359
UCSCiuc009ufe.2 mouse [Q9R0Q1-1]
uc009uff.2 mouse [Q9R0Q1-2]
uc009ufh.3 mouse [Q9R0Q1-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYTL4_MOUSE
AccessioniPrimary (citable) accession number: Q9R0Q1
Secondary accession number(s): B1AVI8
, B1AVI9, Q8R321, Q9R0Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health