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Protein

Synaptotagmin-like protein 4

Gene

Sytl4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 105FYVE-typeAdd BLAST43

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: GO_Central
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: GO_Central
  • neurexin family protein binding Source: UniProtKB
  • phospholipid binding Source: MGI
  • syntaxin binding Source: GO_Central
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-like protein 4
Alternative name(s):
Exophilin-2
Granuphilin
Gene namesi
Name:Sytl4
Synonyms:Slp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1351606. Sytl4.

Subcellular locationi

  • Membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Cytoplasmic vesiclesecretory vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles.

GO - Cellular componenti

  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • endosome Source: MGI
  • exocytic vesicle Source: GO_Central
  • extrinsic component of membrane Source: UniProtKB
  • nucleoplasm Source: MGI
  • plasma membrane Source: GO_Central
  • presynapse Source: GOC
  • secretory granule Source: MGI
  • transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14E → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi18I → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi21V → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi32D → A: Abolishes interaction with RAB27A. 1 Publication1
Mutagenesisi43L → A: Strongly reduces interaction with STX1A. 1 Publication1
Mutagenesisi118W → S: Strongly reduces interaction with RAB27A. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902171 – 673Synaptotagmin-like protein 4Add BLAST673

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei202PhosphoserineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei218PhosphoserineCombined sources1
Modified residuei222PhosphoserineBy similarity1
Modified residuei275PhosphoserineCombined sources1
Modified residuei490PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9R0Q1.
PRIDEiQ9R0Q1.

PTM databases

iPTMnetiQ9R0Q1.
PhosphoSitePlusiQ9R0Q1.

Expressioni

Tissue specificityi

Detected in the pancreatic islet, in particular in insulin-positive beta cells, and in pituitary.1 Publication

Gene expression databases

BgeeiENSMUSG00000031255.
CleanExiMM_SYTL4.
ExpressionAtlasiQ9R0Q1. baseline and differential.
GenevisibleiQ9R0Q1. MM.

Interactioni

Subunit structurei

Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B. Interacts with MYO5A.6 Publications

GO - Molecular functioni

  • clathrin binding Source: GO_Central
  • neurexin family protein binding Source: UniProtKB
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

DIPiDIP-31497N.
IntActiQ9R0Q1. 3 interactors.
STRINGi10090.ENSMUSP00000033608.

Structurei

3D structure databases

ProteinModelPortaliQ9R0Q1.
SMRiQ9R0Q1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 122RabBDPROSITE-ProRule annotationAdd BLAST119
Domaini360 – 464C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini515 – 619C2 2PROSITE-ProRule annotationAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi306 – 313Poly-Glu8

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.Curated
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 105FYVE-typeAdd BLAST43

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XRME. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000231332.
HOVERGENiHBG054255.
InParanoidiQ9R0Q1.
KOiK17598.
OMAiDWFYDQR.
OrthoDBiEOG091G07OO.
PhylomeDBiQ9R0Q1.
TreeFamiTF341184.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028694. SYTL4.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF9. PTHR10024:SF9. 3 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0Q1-1) [UniParc]FASTAAdd to basket
Also known as: Granuphilin-a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEILDLSFL SEMERDLILG VLQRDEELRK ADEKRIRRLK NELLEIKRKG
60 70 80 90 100
AKRGSQHYSD RTCARCQEGL GRLIPKSSTC VGCNHLVCRE CRVLESNGSW
110 120 130 140 150
RCKVCSKEIE LKKATGDWFY DQKVNRFDYR TGSEIIRMSL RQKPAVNKRE
160 170 180 190 200
TAGQSLLQQT QMGDIWPGRR IIQEQQQREQ SVLFEVPKTR SGKSALEAES
210 220 230 240 250
ESLDSYTADS DSTSRRDSLD KSGLFPEWKK MSAPKSQVEK EIPPGNQNAV
260 270 280 290 300
CGDEGDMVFK KNTKKVLRPS EYTKSVIDLR PEDVAQESGI LGDRSKSVPG
310 320 330 340 350
LSVDMEEEEE EEEDIDHLVK LHRQKLARGS MQSGSSMSTL GSIMSIYSEA
360 370 380 390 400
GDFGNISVTG KIAFSLKFEQ KTQTLVIHVK ECHQLAYADE AKKRSNPYVK
410 420 430 440 450
TYLLPDKSRQ GKRKTSIKRD TINPLYDETF RYEISESLLA QRTLQFSVWH
460 470 480 490 500
HGRFGRNTFL GEAEVHMDSW KLDKKLDHCL PLHGKISTES SPGLPAHKGE
510 520 530 540 550
LVVSLKYIPA SKLPVGGDRK KSKGGEGGEL QVWIKEAKNL TAAKSGGTSD
560 570 580 590 600
SFVKGYLLPM RNKASKRKTP VMKKTLSPHY NHTFVYNGVR LEDLQHMCLE
610 620 630 640 650
LTVWDREPLA SNDFLGGVRL GVGTGISNGE VVDWMDSTGE EVSLWQKMRQ
660 670
YPGSWAEGTL QLRSSMVKQK LGV
Length:673
Mass (Da):76,021
Last modified:May 1, 2000 - v1
Checksum:i2AC218E2A4BE4C8D
GO
Isoform 2 (identifier: Q9R0Q1-2) [UniParc]FASTAAdd to basket
Also known as: Granuphilin-b

The sequence of this isoform differs from the canonical sequence as follows:
     486-502: ISTESSPGLPAHKGELV → GSVMAKWWTGWIRLVKK
     503-673: Missing.

Show »
Length:502
Mass (Da):57,479
Checksum:i6353F899E39192A4
GO
Isoform 3 (identifier: Q9R0Q1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     486-673: ISTESSPGLP...SSMVKQKLGV → VVCFKGRSQLASQVNCL

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:502
Mass (Da):57,285
Checksum:i7EC60E5235FF9B0E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007902486 – 673ISTES…QKLGV → VVCFKGRSQLASQVNCL in isoform 3. 1 PublicationAdd BLAST188
Alternative sequenceiVSP_007900486 – 502ISTES…KGELV → GSVMAKWWTGWIRLVKK in isoform 2. 2 PublicationsAdd BLAST17
Alternative sequenceiVSP_007901503 – 673Missing in isoform 2. 2 PublicationsAdd BLAST171

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025258 mRNA. Translation: BAA84656.1.
AB025259 mRNA. Translation: BAA84657.1.
AK030401 mRNA. Translation: BAC26945.1.
AL691421 Genomic DNA. Translation: CAM17840.1.
AL691421 Genomic DNA. Translation: CAM17841.1.
BC026819 mRNA. Translation: AAH26819.1.
CCDSiCCDS30388.1. [Q9R0Q1-1]
CCDS72429.1. [Q9R0Q1-2]
CCDS81169.1. [Q9R0Q1-3]
RefSeqiNP_001277646.1. NM_001290717.1. [Q9R0Q1-2]
NP_001277647.1. NM_001290718.1. [Q9R0Q1-2]
NP_001277648.1. NM_001290719.1. [Q9R0Q1-3]
NP_038785.1. NM_013757.2. [Q9R0Q1-1]
XP_006528614.1. XM_006528551.2. [Q9R0Q1-1]
UniGeneiMm.38674.

Genome annotation databases

EnsembliENSMUST00000033608; ENSMUSP00000033608; ENSMUSG00000031255. [Q9R0Q1-1]
ENSMUST00000113294; ENSMUSP00000108919; ENSMUSG00000031255. [Q9R0Q1-3]
ENSMUST00000113297; ENSMUSP00000108922; ENSMUSG00000031255. [Q9R0Q1-2]
GeneIDi27359.
KEGGimmu:27359.
UCSCiuc009ufe.2. mouse. [Q9R0Q1-1]
uc009uff.2. mouse. [Q9R0Q1-2]
uc009ufh.3. mouse. [Q9R0Q1-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025258 mRNA. Translation: BAA84656.1.
AB025259 mRNA. Translation: BAA84657.1.
AK030401 mRNA. Translation: BAC26945.1.
AL691421 Genomic DNA. Translation: CAM17840.1.
AL691421 Genomic DNA. Translation: CAM17841.1.
BC026819 mRNA. Translation: AAH26819.1.
CCDSiCCDS30388.1. [Q9R0Q1-1]
CCDS72429.1. [Q9R0Q1-2]
CCDS81169.1. [Q9R0Q1-3]
RefSeqiNP_001277646.1. NM_001290717.1. [Q9R0Q1-2]
NP_001277647.1. NM_001290718.1. [Q9R0Q1-2]
NP_001277648.1. NM_001290719.1. [Q9R0Q1-3]
NP_038785.1. NM_013757.2. [Q9R0Q1-1]
XP_006528614.1. XM_006528551.2. [Q9R0Q1-1]
UniGeneiMm.38674.

3D structure databases

ProteinModelPortaliQ9R0Q1.
SMRiQ9R0Q1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-31497N.
IntActiQ9R0Q1. 3 interactors.
STRINGi10090.ENSMUSP00000033608.

PTM databases

iPTMnetiQ9R0Q1.
PhosphoSitePlusiQ9R0Q1.

Proteomic databases

PaxDbiQ9R0Q1.
PRIDEiQ9R0Q1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033608; ENSMUSP00000033608; ENSMUSG00000031255. [Q9R0Q1-1]
ENSMUST00000113294; ENSMUSP00000108919; ENSMUSG00000031255. [Q9R0Q1-3]
ENSMUST00000113297; ENSMUSP00000108922; ENSMUSG00000031255. [Q9R0Q1-2]
GeneIDi27359.
KEGGimmu:27359.
UCSCiuc009ufe.2. mouse. [Q9R0Q1-1]
uc009uff.2. mouse. [Q9R0Q1-2]
uc009ufh.3. mouse. [Q9R0Q1-3]

Organism-specific databases

CTDi94121.
MGIiMGI:1351606. Sytl4.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XRME. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000231332.
HOVERGENiHBG054255.
InParanoidiQ9R0Q1.
KOiK17598.
OMAiDWFYDQR.
OrthoDBiEOG091G07OO.
PhylomeDBiQ9R0Q1.
TreeFamiTF341184.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Miscellaneous databases

PROiQ9R0Q1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031255.
CleanExiMM_SYTL4.
ExpressionAtlasiQ9R0Q1. baseline and differential.
GenevisibleiQ9R0Q1. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR010911. Rab_BD.
IPR028694. SYTL4.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF9. PTHR10024:SF9. 3 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50004. C2. 2 hits.
PS50916. RABBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYTL4_MOUSE
AccessioniPrimary (citable) accession number: Q9R0Q1
Secondary accession number(s): B1AVI8
, B1AVI9, Q8R321, Q9R0Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.