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Protein

Destrin

Gene

Dstn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.

GO - Molecular functioni

  • actin filament binding Source: UniProtKB

GO - Biological processi

  • actin filament depolymerization Source: UniProtKB
  • actin filament fragmentation Source: UniProtKB
  • actin filament severing Source: InterPro
  • positive regulation of actin filament depolymerization Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Destrin
Alternative name(s):
Actin-depolymerizing factor
Short name:
ADF
Sid 23
Gene namesi
Name:Dstn
Synonyms:Dsn, Sid23
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1929270. Dstn.

Subcellular locationi

GO - Cellular componenti

  • cortical actin cytoskeleton Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 165164DestrinPRO_0000214919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei3 – 31PhosphoserineCombined sources
Modified residuei19 – 191N6-acetyllysineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9R0P5.
MaxQBiQ9R0P5.
PaxDbiQ9R0P5.
PRIDEiQ9R0P5.

2D gel databases

REPRODUCTION-2DPAGEIPI00127942.
Q9R0P5.

PTM databases

iPTMnetiQ9R0P5.
PhosphoSiteiQ9R0P5.
SwissPalmiQ9R0P5.

Expressioni

Tissue specificityi

Widely expressed. Not found in skeletal muscle.1 Publication

Developmental stagei

In E10.5 embryo somites is expressed in a superficial patch of cells (adaxial region).1 Publication

Gene expression databases

BgeeiENSMUSG00000015932.
CleanExiMM_DSTN.
ExpressionAtlasiQ9R0P5. baseline and differential.
GenevisibleiQ9R0P5. MM.

Interactioni

GO - Molecular functioni

  • actin filament binding Source: UniProtKB

Protein-protein interaction databases

BioGridi207975. 1 interaction.
IntActiQ9R0P5. 7 interactions.
MINTiMINT-1869015.
STRINGi10090.ENSMUSP00000099461.

Structurei

3D structure databases

ProteinModelPortaliQ9R0P5.
SMRiQ9R0P5. Positions 2-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 153150ADF-HPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi30 – 345Nuclear localization signalSequence analysis

Sequence similaritiesi

Belongs to the actin-binding proteins ADF family.Curated
Contains 1 ADF-H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1735. Eukaryota.
ENOG41122P5. LUCA.
GeneTreeiENSGT00440000033289.
HOGENOMiHOG000039697.
HOVERGENiHBG000381.
InParanoidiQ9R0P5.
KOiK10363.
OMAiKHEYQAN.
OrthoDBiEOG091G0PWC.
PhylomeDBiQ9R0P5.
TreeFamiTF328601.

Family and domain databases

CDDicd11286. ADF_cofilin_like. 1 hit.
Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR017904. ADF/Cofilin.
IPR029924. Dstn.
[Graphical view]
PANTHERiPTHR11913. PTHR11913. 1 hit.
PTHR11913:SF18. PTHR11913:SF18. 1 hit.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
PRINTSiPR00006. COFILIN.
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0P5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIVVE
60 70 80 90 100
EGKEILVGDV GATITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM
110 120 130 140 150
FFLWAPEQAP LKSKMIYASS KDAIKKKFPG IKHEYQANGP EDLNRTCIAE
160
KLGGSLIVAF EGSPV
Length:165
Mass (Da):18,522
Last modified:January 23, 2007 - v3
Checksum:i42BD07984C9B3667
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025406 mRNA. Translation: BAA84691.1.
AK078898 mRNA. Translation: BAC37447.1.
CCDSiCCDS16812.1.
RefSeqiNP_062745.1. NM_019771.2.
UniGeneiMm.28919.

Genome annotation databases

EnsembliENSMUST00000103172; ENSMUSP00000099461; ENSMUSG00000015932.
GeneIDi56431.
KEGGimmu:56431.
UCSCiuc008mqi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025406 mRNA. Translation: BAA84691.1.
AK078898 mRNA. Translation: BAC37447.1.
CCDSiCCDS16812.1.
RefSeqiNP_062745.1. NM_019771.2.
UniGeneiMm.28919.

3D structure databases

ProteinModelPortaliQ9R0P5.
SMRiQ9R0P5. Positions 2-165.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207975. 1 interaction.
IntActiQ9R0P5. 7 interactions.
MINTiMINT-1869015.
STRINGi10090.ENSMUSP00000099461.

PTM databases

iPTMnetiQ9R0P5.
PhosphoSiteiQ9R0P5.
SwissPalmiQ9R0P5.

2D gel databases

REPRODUCTION-2DPAGEIPI00127942.
Q9R0P5.

Proteomic databases

EPDiQ9R0P5.
MaxQBiQ9R0P5.
PaxDbiQ9R0P5.
PRIDEiQ9R0P5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103172; ENSMUSP00000099461; ENSMUSG00000015932.
GeneIDi56431.
KEGGimmu:56431.
UCSCiuc008mqi.2. mouse.

Organism-specific databases

CTDi11034.
MGIiMGI:1929270. Dstn.

Phylogenomic databases

eggNOGiKOG1735. Eukaryota.
ENOG41122P5. LUCA.
GeneTreeiENSGT00440000033289.
HOGENOMiHOG000039697.
HOVERGENiHBG000381.
InParanoidiQ9R0P5.
KOiK10363.
OMAiKHEYQAN.
OrthoDBiEOG091G0PWC.
PhylomeDBiQ9R0P5.
TreeFamiTF328601.

Miscellaneous databases

PROiQ9R0P5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015932.
CleanExiMM_DSTN.
ExpressionAtlasiQ9R0P5. baseline and differential.
GenevisibleiQ9R0P5. MM.

Family and domain databases

CDDicd11286. ADF_cofilin_like. 1 hit.
Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR017904. ADF/Cofilin.
IPR029924. Dstn.
[Graphical view]
PANTHERiPTHR11913. PTHR11913. 1 hit.
PTHR11913:SF18. PTHR11913:SF18. 1 hit.
PfamiPF00241. Cofilin_ADF. 1 hit.
[Graphical view]
PRINTSiPR00006. COFILIN.
SMARTiSM00102. ADF. 1 hit.
[Graphical view]
PROSITEiPS51263. ADF_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEST_MOUSE
AccessioniPrimary (citable) accession number: Q9R0P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.