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Protein

Synaptotagmin-9

Gene

Syt9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi251Calcium 1By similarity1
Metal bindingi251Calcium 2By similarity1
Metal bindingi257Calcium 1By similarity1
Metal bindingi309Calcium 1By similarity1
Metal bindingi309Calcium 2By similarity1
Metal bindingi310Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi311Calcium 1By similarity1
Metal bindingi311Calcium 2By similarity1
Metal bindingi311Calcium 3By similarity1
Metal bindingi314Calcium 3By similarity1
Metal bindingi317Calcium 2By similarity1
Metal bindingi317Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: GO_Central
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: GO_Central
  • phosphatidylinositol-4,5-bisphosphate binding Source: ParkinsonsUK-UCL
  • phosphatidylserine binding Source: ParkinsonsUK-UCL
  • SNARE binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-9
Alternative name(s):
Synaptotagmin IX
Short name:
SytIX
Synaptotagmin V1 Publication
Gene namesi
Name:Syt9
Synonyms:Syt51 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1926373. Syt9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 52VesicularSequence analysisAdd BLAST52
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 491CytoplasmicSequence analysisAdd BLAST418

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839631 – 491Synaptotagmin-9Add BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei132PhosphothreonineBy similarity1
Modified residuei177PhosphoserineCombined sources1
Modified residuei207PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiQ9R0N9.
PaxDbiQ9R0N9.
PeptideAtlasiQ9R0N9.
PRIDEiQ9R0N9.

PTM databases

iPTMnetiQ9R0N9.
PhosphoSitePlusiQ9R0N9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000062542.
CleanExiMM_SYT5.
MM_SYT9.
ExpressionAtlasiQ9R0N9. baseline and differential.
GenevisibleiQ9R0N9. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked via the cysteine motif (PubMed:10531343). Can also form heterodimers with SYT3, SYT6, SYT7 and SYT10 (PubMed:10531343, PubMed:10531344, PubMed:10871604).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-458006,EBI-458006
Syt10Q9R0N42EBI-458006,EBI-5239459
Syt3O356812EBI-458006,EBI-457995
Syt6Q9R0N82EBI-458006,EBI-5239378

GO - Molecular functioni

  • clathrin binding Source: GO_Central
  • SNARE binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

IntActiQ9R0N9. 4 interactors.
STRINGi10090.ENSMUSP00000073164.

Structurei

3D structure databases

ProteinModelPortaliQ9R0N9.
SMRiQ9R0N9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 323C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini354 – 457C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 31Cysteine motifBy similarityAdd BLAST23

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XQZ9. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiQ9R0N9.
KOiK19909.
OMAiDFPRECN.
OrthoDBiEOG091G0XMQ.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028691. SYT9.
[Graphical view]
PANTHERiPTHR10024:SF180. PTHR10024:SF180. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0N9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGARDALCH QALQLLAELC ARGALEHDSC QDFIYHLRDR ARPRLRDPDI
60 70 80 90 100
SVSLLTLVVT ACGLALFGVS LFVSWKLCWV PWRERGLFSG SKDNNQEPLN
110 120 130 140 150
YTDTETNEQE NSEDFLDPPT PCPDSSMKIS HTSPDIPLST QPGGQENCAH
160 170 180 190 200
AVRVQRQVTE PTPSARHNSI RRQLNLSNPD FNIQQLQRQE QLTGIGRIKP
210 220 230 240 250
ELYKQRSLDN DDGRRSNSKA CGKLNFILKY DCDLEQLIVK IHKAVNLPAK
260 270 280 290 300
DFSGTSDPYV KIYLLPDRKT KHQTKVHRKT LNPVFDEVFL FPVHYNDLEA
310 320 330 340 350
RKLHFSVYDF DRFSRHDLIG QVVVDHFFDL ADFPRECILW KDIEYVTNDN
360 370 380 390 400
VDLGELMFSL CYLPTAGRLT ITIIKARNLK AMDITGASDP YVKVSLMCDG
410 420 430 440 450
RRLKKRKTST KRNTLNPVYN EAIVFDVPPE SIDQIHLSIA VMDYDRVGHN
460 470 480 490
EVIGVCQVGN EAERLGRDHW SEMLSYPRKP IAHWHSLMEK R
Length:491
Mass (Da):56,265
Last modified:July 27, 2011 - v2
Checksum:iE08ECFD9F4D6EE14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti488M → L in BAA85774 (PubMed:10531343).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026802 mRNA. Translation: BAA85774.1.
AK089115 mRNA. Translation: BAC40759.1.
AK156631 mRNA. Translation: BAE33784.1.
CH466531 Genomic DNA. Translation: EDL16861.1.
BC132495 mRNA. Translation: AAI32496.1.
BC137904 mRNA. Translation: AAI37905.1.
CCDSiCCDS40078.1.
RefSeqiNP_068689.2. NM_021889.4.
UniGeneiMm.302793.

Genome annotation databases

EnsembliENSMUST00000073459; ENSMUSP00000073164; ENSMUSG00000062542.
GeneIDi60510.
KEGGimmu:60510.
UCSCiuc009jba.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026802 mRNA. Translation: BAA85774.1.
AK089115 mRNA. Translation: BAC40759.1.
AK156631 mRNA. Translation: BAE33784.1.
CH466531 Genomic DNA. Translation: EDL16861.1.
BC132495 mRNA. Translation: AAI32496.1.
BC137904 mRNA. Translation: AAI37905.1.
CCDSiCCDS40078.1.
RefSeqiNP_068689.2. NM_021889.4.
UniGeneiMm.302793.

3D structure databases

ProteinModelPortaliQ9R0N9.
SMRiQ9R0N9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R0N9. 4 interactors.
STRINGi10090.ENSMUSP00000073164.

PTM databases

iPTMnetiQ9R0N9.
PhosphoSitePlusiQ9R0N9.

Proteomic databases

MaxQBiQ9R0N9.
PaxDbiQ9R0N9.
PeptideAtlasiQ9R0N9.
PRIDEiQ9R0N9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073459; ENSMUSP00000073164; ENSMUSG00000062542.
GeneIDi60510.
KEGGimmu:60510.
UCSCiuc009jba.1. mouse.

Organism-specific databases

CTDi143425.
MGIiMGI:1926373. Syt9.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG410XQZ9. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiQ9R0N9.
KOiK19909.
OMAiDFPRECN.
OrthoDBiEOG091G0XMQ.
TreeFamiTF315600.

Enzyme and pathway databases

ReactomeiR-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ9R0N9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062542.
CleanExiMM_SYT5.
MM_SYT9.
ExpressionAtlasiQ9R0N9. baseline and differential.
GenevisibleiQ9R0N9. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028691. SYT9.
[Graphical view]
PANTHERiPTHR10024:SF180. PTHR10024:SF180. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYT9_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N9
Secondary accession number(s): Q3U0R7, Q8C280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.