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Protein

Synaptotagmin-9

Gene

Syt9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi251 – 2511Calcium 1By similarity
Metal bindingi251 – 2511Calcium 2By similarity
Metal bindingi257 – 2571Calcium 1By similarity
Metal bindingi309 – 3091Calcium 1By similarity
Metal bindingi309 – 3091Calcium 2By similarity
Metal bindingi310 – 3101Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi311 – 3111Calcium 1By similarity
Metal bindingi311 – 3111Calcium 2By similarity
Metal bindingi311 – 3111Calcium 3By similarity
Metal bindingi314 – 3141Calcium 3By similarity
Metal bindingi317 – 3171Calcium 2By similarity
Metal bindingi317 – 3171Calcium 3By similarity

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. metal ion binding Source: UniProtKB-KW
  3. transporter activity Source: InterPro

GO - Biological processi

  1. positive regulation of calcium ion-dependent exocytosis Source: Ensembl
  2. regulation of insulin secretion Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-9
Alternative name(s):
Synaptotagmin IX
Short name:
SytIX
Synaptotagmin V
Gene namesi
Name:Syt9
Synonyms:Syt5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1926373. Syt9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252VesicularSequence AnalysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence AnalysisAdd
BLAST
Topological domaini74 – 491418CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. dense core granule Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. secretory granule membrane Source: Ensembl
  5. synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Synaptotagmin-9PRO_0000183963Add
BLAST

Proteomic databases

PaxDbiQ9R0N9.
PRIDEiQ9R0N9.

PTM databases

PhosphoSiteiQ9R0N9.

Expressioni

Gene expression databases

BgeeiQ9R0N9.
CleanExiMM_SYT5.
MM_SYT9.
ExpressionAtlasiQ9R0N9. baseline and differential.
GenevestigatoriQ9R0N9.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-458006,EBI-458006
Syt10Q9R0N42EBI-458006,EBI-5239459
Syt3O356812EBI-458006,EBI-457995
Syt6Q9R0N82EBI-458006,EBI-5239378

Protein-protein interaction databases

IntActiQ9R0N9. 4 interactions.
STRINGi10090.ENSMUSP00000073164.

Structurei

3D structure databases

ProteinModelPortaliQ9R0N9.
SMRiQ9R0N9. Positions 221-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini222 – 323102C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini354 – 457104C2 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292488.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiQ9R0N9.
OMAiDFPRECV.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028691. SYT9.
[Graphical view]
PANTHERiPTHR10024:SF174. PTHR10024:SF174. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0N9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGARDALCH QALQLLAELC ARGALEHDSC QDFIYHLRDR ARPRLRDPDI
60 70 80 90 100
SVSLLTLVVT ACGLALFGVS LFVSWKLCWV PWRERGLFSG SKDNNQEPLN
110 120 130 140 150
YTDTETNEQE NSEDFLDPPT PCPDSSMKIS HTSPDIPLST QPGGQENCAH
160 170 180 190 200
AVRVQRQVTE PTPSARHNSI RRQLNLSNPD FNIQQLQRQE QLTGIGRIKP
210 220 230 240 250
ELYKQRSLDN DDGRRSNSKA CGKLNFILKY DCDLEQLIVK IHKAVNLPAK
260 270 280 290 300
DFSGTSDPYV KIYLLPDRKT KHQTKVHRKT LNPVFDEVFL FPVHYNDLEA
310 320 330 340 350
RKLHFSVYDF DRFSRHDLIG QVVVDHFFDL ADFPRECILW KDIEYVTNDN
360 370 380 390 400
VDLGELMFSL CYLPTAGRLT ITIIKARNLK AMDITGASDP YVKVSLMCDG
410 420 430 440 450
RRLKKRKTST KRNTLNPVYN EAIVFDVPPE SIDQIHLSIA VMDYDRVGHN
460 470 480 490
EVIGVCQVGN EAERLGRDHW SEMLSYPRKP IAHWHSLMEK R
Length:491
Mass (Da):56,265
Last modified:July 27, 2011 - v2
Checksum:iE08ECFD9F4D6EE14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti488 – 4881M → L in BAA85774. (PubMed:10531343)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026802 mRNA. Translation: BAA85774.1.
AK089115 mRNA. Translation: BAC40759.1.
AK156631 mRNA. Translation: BAE33784.1.
CH466531 Genomic DNA. Translation: EDL16861.1.
BC132495 mRNA. Translation: AAI32496.1.
BC137904 mRNA. Translation: AAI37905.1.
CCDSiCCDS40078.1.
RefSeqiNP_068689.2. NM_021889.4.
UniGeneiMm.302793.

Genome annotation databases

EnsembliENSMUST00000073459; ENSMUSP00000073164; ENSMUSG00000062542.
GeneIDi60510.
KEGGimmu:60510.
UCSCiuc009jba.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026802 mRNA. Translation: BAA85774.1.
AK089115 mRNA. Translation: BAC40759.1.
AK156631 mRNA. Translation: BAE33784.1.
CH466531 Genomic DNA. Translation: EDL16861.1.
BC132495 mRNA. Translation: AAI32496.1.
BC137904 mRNA. Translation: AAI37905.1.
CCDSiCCDS40078.1.
RefSeqiNP_068689.2. NM_021889.4.
UniGeneiMm.302793.

3D structure databases

ProteinModelPortaliQ9R0N9.
SMRiQ9R0N9. Positions 221-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R0N9. 4 interactions.
STRINGi10090.ENSMUSP00000073164.

PTM databases

PhosphoSiteiQ9R0N9.

Proteomic databases

PaxDbiQ9R0N9.
PRIDEiQ9R0N9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073459; ENSMUSP00000073164; ENSMUSG00000062542.
GeneIDi60510.
KEGGimmu:60510.
UCSCiuc009jba.1. mouse.

Organism-specific databases

CTDi143425.
MGIiMGI:1926373. Syt9.

Phylogenomic databases

eggNOGiNOG292488.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiQ9R0N9.
OMAiDFPRECV.
TreeFamiTF315600.

Miscellaneous databases

NextBioi314917.
PROiQ9R0N9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0N9.
CleanExiMM_SYT5.
MM_SYT9.
ExpressionAtlasiQ9R0N9. baseline and differential.
GenevestigatoriQ9R0N9.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028691. SYT9.
[Graphical view]
PANTHERiPTHR10024:SF174. PTHR10024:SF174. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
    Fukuda M., Kanno E., Mikoshiba K.
    J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Cerebellum.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Olfactory bulb and Spleen.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiSYT9_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N9
Secondary accession number(s): Q3U0R7, Q8C280
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 27, 2011
Last modified: January 7, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.