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Q9R0N8

- SYT6_MOUSE

UniProt

Q9R0N8 - SYT6_MOUSE

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Protein

Synaptotagmin-6

Gene

Syt6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis (By similarity). May mediate Ca2+-regulation of exocytosis in acrosomal reaction in sperm.By similarity1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi261 – 2611Calcium 1By similarity
Metal bindingi261 – 2611Calcium 2By similarity
Metal bindingi267 – 2671Calcium 1By similarity
Metal bindingi319 – 3191Calcium 1By similarity
Metal bindingi319 – 3191Calcium 2By similarity
Metal bindingi320 – 3201Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi321 – 3211Calcium 1By similarity
Metal bindingi321 – 3211Calcium 2By similarity
Metal bindingi321 – 3211Calcium 3By similarity
Metal bindingi324 – 3241Calcium 3By similarity
Metal bindingi327 – 3271Calcium 2By similarity
Metal bindingi327 – 3271Calcium 3By similarity

GO - Molecular functioni

  1. calcium-dependent protein binding Source: UniProtKB
  2. identical protein binding Source: IntAct
  3. metal ion binding Source: UniProtKB-KW
  4. protein heterodimerization activity Source: UniProtKB
  5. protein homodimerization activity Source: BHF-UCL
  6. transporter activity Source: InterPro

GO - Biological processi

  1. acrosomal vesicle exocytosis Source: Ensembl
  2. acrosome reaction Source: HGNC
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-6
Alternative name(s):
Synaptotagmin VI
Short name:
SytVI
Gene namesi
Name:Syt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1859544. Syt6.

Subcellular locationi

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass membrane protein 1 Publication
Isoform 1 : Membrane; Single-pass membrane protein
Note: Localized predominantly to endoplasmic reticulum (ER) and/or Golgi-like perinuclear compartment.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5959VesicularSequence AnalysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Topological domaini81 – 511431CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytosol Source: UniProtKB
  3. extrinsic component of membrane Source: UniProtKB
  4. integral component of membrane Source: BHF-UCL
  5. perinuclear endoplasmic reticulum Source: BHF-UCL
  6. plasma membrane Source: UniProtKB
  7. synaptic vesicle Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Synaptotagmin-6PRO_0000183955Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei217 – 2171PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9R0N8.
PaxDbiQ9R0N8.
PRIDEiQ9R0N8.

PTM databases

PhosphoSiteiQ9R0N8.

Expressioni

Tissue specificityi

Isoform 1 is expressed in the olfactory bulb. Isoform 2 is expressed in the brain (at protein level).

Gene expression databases

BgeeiQ9R0N8.
CleanExiMM_SYT6.
ExpressionAtlasiQ9R0N8. baseline and differential.
GenevestigatoriQ9R0N8.

Interactioni

Subunit structurei

Homodimer (isoform 1). Isoform 1 forms heterodimers with SytIII, SytV and SytX. Interacts with STX1A, STX1B and STX2; the interaction is Ca2+-dependent. Isoform 2 is not able to form homodimer and heterodimers.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-5239378,EBI-5239378
Syt10Q9R0N42EBI-5239378,EBI-5239459
Syt3O356812EBI-5239378,EBI-457995
Syt9Q9R0N92EBI-5239378,EBI-458006

Protein-protein interaction databases

IntActiQ9R0N8. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9R0N8.
SMRiQ9R0N8. Positions 231-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini232 – 333102C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini364 – 467104C2 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni483 – 51129Necessary for cell membrane association (isoform 2)Add
BLAST

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5038.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiQ9R0N8.
OMAiWRDIQYA.
OrthoDBiEOG78PV8W.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028701. SYT6.
[Graphical view]
PANTHERiPTHR10024:SF45. PTHR10024:SF45. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9R0N8-1) [UniParc]FASTAAdd to Basket

Also known as: SytVI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGVWGAGGP RCQAALAVLA SLCRARPPPL GLDVETCRSF ELQSPEQSPS
60 70 80 90 100
AADSGTSVSL LAVVVIVCGV ALVAVFLFLF WKLCWMPWRK KEASSPSSAN
110 120 130 140 150
PASETLQSPS SRGNMADKLK DPSALGFLEA AVKISHTSPD IPAEVQMSVK
160 170 180 190 200
EHIMRHTKLQ RQTTEPASST RHTSFKRHLP RQMHVSSVDY GNELPPAAAE
210 220 230 240 250
QPTSIGRIKP ELYKQKSVDG DDAKSEAAKS CGKINFSLRY DYESETLIVR
260 270 280 290 300
ILKAFDLPAK DFCGSSDPYV KIYLLPDRKC KLQTRVHRKT LNPTFDENFH
310 320 330 340 350
FPVPYEELAD RKLHLSVFDF DRFSRHDMIG EVILDNLFEA SDLSRETSIW
360 370 380 390 400
KDIQYATSES VDLGEIMFSL CYLPTAGRLT LTVIKCRNLK AMDITGYSDP
410 420 430 440 450
YVKVSLLCDG RRLKKKKTTI KKNTLNPIYN EAIIFDIPPE NMDQVSLLIS
460 470 480 490 500
VMDYDRVGHN EIIGVCRVGI NAEGLGRDHW NEMLAYPRKP IAHWHSLVEV
510
KKSFKEGTPR L
Length:511
Mass (Da):57,204
Last modified:July 27, 2011 - v2
Checksum:iC37C9305E3D05572
GO
Isoform 2 (identifier: Q9R0N8-2) [UniParc]FASTAAdd to Basket

Also known as: SytVIdeltaTM1

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Show »
Length:426
Mass (Da):48,343
Checksum:i699B22C7B06C3179
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti428 – 4281I → V in BAA85775. (PubMed:10531343)Curated
Sequence conflicti428 – 4281I → V in BAA85781. (PubMed:10531344)Curated
Sequence conflicti428 – 4281I → V in BAA85782. (PubMed:10531344)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8585Missing in isoform 2. 1 PublicationVSP_041729Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026803 mRNA. Translation: BAA85775.1.
AB026809 mRNA. Translation: BAA85781.1.
AB026810 mRNA. Translation: BAA85782.1.
AC123057 Genomic DNA. No translation available.
CCDSiCCDS38575.1. [Q9R0N8-1]
CCDS71296.1. [Q9R0N8-2]
RefSeqiNP_061270.2. NM_018800.4. [Q9R0N8-1]
UniGeneiMm.488213.
Mm.88818.

Genome annotation databases

EnsembliENSMUST00000090697; ENSMUSP00000088196; ENSMUSG00000027849. [Q9R0N8-1]
ENSMUST00000117221; ENSMUSP00000113373; ENSMUSG00000027849. [Q9R0N8-2]
ENSMUST00000121834; ENSMUSP00000112997; ENSMUSG00000027849. [Q9R0N8-1]
GeneIDi54524.
KEGGimmu:54524.
UCSCiuc008qsy.1. mouse. [Q9R0N8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026803 mRNA. Translation: BAA85775.1 .
AB026809 mRNA. Translation: BAA85781.1 .
AB026810 mRNA. Translation: BAA85782.1 .
AC123057 Genomic DNA. No translation available.
CCDSi CCDS38575.1. [Q9R0N8-1 ]
CCDS71296.1. [Q9R0N8-2 ]
RefSeqi NP_061270.2. NM_018800.4. [Q9R0N8-1 ]
UniGenei Mm.488213.
Mm.88818.

3D structure databases

ProteinModelPortali Q9R0N8.
SMRi Q9R0N8. Positions 231-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9R0N8. 3 interactions.

PTM databases

PhosphoSitei Q9R0N8.

Proteomic databases

MaxQBi Q9R0N8.
PaxDbi Q9R0N8.
PRIDEi Q9R0N8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000090697 ; ENSMUSP00000088196 ; ENSMUSG00000027849 . [Q9R0N8-1 ]
ENSMUST00000117221 ; ENSMUSP00000113373 ; ENSMUSG00000027849 . [Q9R0N8-2 ]
ENSMUST00000121834 ; ENSMUSP00000112997 ; ENSMUSG00000027849 . [Q9R0N8-1 ]
GeneIDi 54524.
KEGGi mmu:54524.
UCSCi uc008qsy.1. mouse. [Q9R0N8-1 ]

Organism-specific databases

CTDi 148281.
MGIi MGI:1859544. Syt6.

Phylogenomic databases

eggNOGi COG5038.
GeneTreei ENSGT00760000118973.
HOGENOMi HOG000232128.
HOVERGENi HBG005010.
InParanoidi Q9R0N8.
OMAi WRDIQYA.
OrthoDBi EOG78PV8W.
TreeFami TF315600.

Miscellaneous databases

ChiTaRSi Syt6. mouse.
NextBioi 311360.
PROi Q9R0N8.
SOURCEi Search...

Gene expression databases

Bgeei Q9R0N8.
CleanExi MM_SYT6.
ExpressionAtlasi Q9R0N8. baseline and differential.
Genevestigatori Q9R0N8.

Family and domain databases

Gene3Di 2.60.40.150. 2 hits.
InterProi IPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028701. SYT6.
[Graphical view ]
PANTHERi PTHR10024:SF45. PTHR10024:SF45. 1 hit.
Pfami PF00168. C2. 2 hits.
[Graphical view ]
PRINTSi PR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTi SM00239. C2. 2 hits.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 2 hits.
PROSITEi PS50004. C2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
    Fukuda M., Kanno E., Mikoshiba K.
    J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: ICR.
    Tissue: Cerebellum.
  2. "A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
    Fukuda M., Mikoshiba K.
    J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION (ISOFORMS 1 AND 2), HOMODIMERIZATION, HETERODIMERIZATION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
    Strain: ICR.
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction."
    Hutt D.M., Baltz J.M., Ngsee J.K.
    J. Biol. Chem. 280:20197-20203(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STX1A; STX1B AND STX2.

Entry informationi

Entry nameiSYT6_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N8
Secondary accession number(s): E9QJV2, Q9QUK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3