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Q9R0N8 (SYT6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptotagmin-6
Alternative name(s):
Synaptotagmin VI
Short name=SytVI
Gene names
Name:Syt6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis By similarity. May mediate Ca2+-regulation of exocytosis in acrosomal reaction in sperm. Ref.4

Cofactor

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Subunit structure

Homodimer (isoform 1) Isoform 1 forms heterodimers with SytIII, SytV and SytX. Interacts with STX1A, STX1B and STX2; the interaction is Ca2+-dependent. Isoform 2 is not able to form homodimer and heterodimers. Ref.2 Ref.4

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass membrane protein Ref.2.

Isoform 1: Membrane; Single-pass membrane protein. Note: Localized predominantly to endoplasmic reticulum (ER) and/or Golgi-like perinuclear compartment. Ref.2

Isoform 2: Cytoplasmcytosol. Cell membrane; Peripheral membrane protein Ref.2.

Tissue specificity

Isoform 1 is expressed in the olfactory bulb. Isoform 2 is expressed in the brain (at protein level).

Sequence similarities

Belongs to the synaptotagmin family.

Contains 2 C2 domains.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Cytoplasm
Cytoplasmic vesicle
Membrane
Synapse
   Coding sequence diversityAlternative splicing
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandCalcium
Metal-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacrosome reaction

Inferred from direct assay Ref.4. Source: HGNC

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytosol

Inferred from direct assay Ref.2. Source: UniProtKB

extrinsic component of membrane

Inferred from direct assay Ref.2. Source: UniProtKB

integral component of membrane

Inferred from direct assay Ref.2. Source: BHF-UCL

perinuclear endoplasmic reticulum

Inferred from direct assay Ref.2. Source: BHF-UCL

plasma membrane

Inferred from direct assay Ref.2. Source: UniProtKB

synaptic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium-dependent protein binding

Inferred from physical interaction Ref.4. Source: UniProtKB

identical protein binding

Inferred from physical interaction Ref.1. Source: IntAct

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein heterodimerization activity

Inferred from physical interaction Ref.2. Source: UniProtKB

protein homodimerization activity

Inferred from direct assay Ref.1. Source: BHF-UCL

transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9R0N8-1)

Also known as: SytVI;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9R0N8-2)

Also known as: SytVIdeltaTM1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 511511Synaptotagmin-6
PRO_0000183955

Regions

Topological domain1 – 5959Vesicular Potential
Transmembrane60 – 8021Helical; Potential
Topological domain81 – 511431Cytoplasmic Potential
Domain232 – 333102C2 1
Domain364 – 467104C2 2
Region483 – 51129Necessary for cell membrane association (isoform 2)

Sites

Metal binding2611Calcium 1 By similarity
Metal binding2611Calcium 2 By similarity
Metal binding2671Calcium 1 By similarity
Metal binding3191Calcium 1 By similarity
Metal binding3191Calcium 2 By similarity
Metal binding3201Calcium 1; via carbonyl oxygen By similarity
Metal binding3211Calcium 1 By similarity
Metal binding3211Calcium 2 By similarity
Metal binding3211Calcium 3 By similarity
Metal binding3241Calcium 3 By similarity
Metal binding3271Calcium 2 By similarity
Metal binding3271Calcium 3 By similarity

Amino acid modifications

Modified residue2171Phosphoserine By similarity

Natural variations

Alternative sequence1 – 8585Missing in isoform 2.
VSP_041729

Experimental info

Sequence conflict4281I → V in BAA85775. Ref.1
Sequence conflict4281I → V in BAA85781. Ref.2
Sequence conflict4281I → V in BAA85782. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (SytVI) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: C37C9305E3D05572

FASTA51157,204
        10         20         30         40         50         60 
MSGVWGAGGP RCQAALAVLA SLCRARPPPL GLDVETCRSF ELQSPEQSPS AADSGTSVSL 

        70         80         90        100        110        120 
LAVVVIVCGV ALVAVFLFLF WKLCWMPWRK KEASSPSSAN PASETLQSPS SRGNMADKLK 

       130        140        150        160        170        180 
DPSALGFLEA AVKISHTSPD IPAEVQMSVK EHIMRHTKLQ RQTTEPASST RHTSFKRHLP 

       190        200        210        220        230        240 
RQMHVSSVDY GNELPPAAAE QPTSIGRIKP ELYKQKSVDG DDAKSEAAKS CGKINFSLRY 

       250        260        270        280        290        300 
DYESETLIVR ILKAFDLPAK DFCGSSDPYV KIYLLPDRKC KLQTRVHRKT LNPTFDENFH 

       310        320        330        340        350        360 
FPVPYEELAD RKLHLSVFDF DRFSRHDMIG EVILDNLFEA SDLSRETSIW KDIQYATSES 

       370        380        390        400        410        420 
VDLGEIMFSL CYLPTAGRLT LTVIKCRNLK AMDITGYSDP YVKVSLLCDG RRLKKKKTTI 

       430        440        450        460        470        480 
KKNTLNPIYN EAIIFDIPPE NMDQVSLLIS VMDYDRVGHN EIIGVCRVGI NAEGLGRDHW 

       490        500        510 
NEMLAYPRKP IAHWHSLVEV KKSFKEGTPR L 

« Hide

Isoform 2 (SytVIdeltaTM1) [UniParc].

Checksum: 699B22C7B06C3179
Show »

FASTA42648,343

References

« Hide 'large scale' references
[1]"Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
Fukuda M., Kanno E., Mikoshiba K.
J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: ICR.
Tissue: Cerebellum.
[2]"A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
Fukuda M., Mikoshiba K.
J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION (ISOFORMS 1 AND 2), HOMODIMERIZATION, HETERODIMERIZATION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
Strain: ICR.
Tissue: Cerebellum.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction."
Hutt D.M., Baltz J.M., Ngsee J.K.
J. Biol. Chem. 280:20197-20203(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH STX1A; STX1B AND STX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026803 mRNA. Translation: BAA85775.1.
AB026809 mRNA. Translation: BAA85781.1.
AB026810 mRNA. Translation: BAA85782.1.
AC123057 Genomic DNA. No translation available.
RefSeqNP_061270.2. NM_018800.4.
UniGeneMm.488213.
Mm.88818.

3D structure databases

ProteinModelPortalQ9R0N8.
SMRQ9R0N8. Positions 231-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9R0N8. 3 interactions.

PTM databases

PhosphoSiteQ9R0N8.

Proteomic databases

PaxDbQ9R0N8.
PRIDEQ9R0N8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000090697; ENSMUSP00000088196; ENSMUSG00000027849. [Q9R0N8-1]
ENSMUST00000117221; ENSMUSP00000113373; ENSMUSG00000027849. [Q9R0N8-2]
ENSMUST00000121834; ENSMUSP00000112997; ENSMUSG00000027849. [Q9R0N8-1]
GeneID54524.
KEGGmmu:54524.
UCSCuc008qsy.1. mouse. [Q9R0N8-1]

Organism-specific databases

CTD148281.
MGIMGI:1859544. Syt6.

Phylogenomic databases

eggNOGCOG5038.
GeneTreeENSGT00710000106556.
HOGENOMHOG000232128.
HOVERGENHBG005010.
InParanoidQ9QUK7.
OMAEHFLRRT.
OrthoDBEOG78PV8W.
TreeFamTF315600.

Gene expression databases

BgeeQ9R0N8.
CleanExMM_SYT6.
GenevestigatorQ9R0N8.

Family and domain databases

Gene3D2.60.40.150. 2 hits.
InterProIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028701. SYT6.
[Graphical view]
PANTHERPTHR10024:SF45. PTHR10024:SF45. 1 hit.
PfamPF00168. C2. 2 hits.
[Graphical view]
PRINTSPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTSM00239. C2. 2 hits.
[Graphical view]
SUPFAMSSF49562. SSF49562. 2 hits.
PROSITEPS50004. C2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio311360.
PROQ9R0N8.
SOURCESearch...

Entry information

Entry nameSYT6_MOUSE
AccessionPrimary (citable) accession number: Q9R0N8
Secondary accession number(s): E9QJV2, Q9QUK7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot