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Protein

Synaptotagmin-6

Gene

Syt6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis (By similarity). May mediate Ca2+-regulation of exocytosis in acrosomal reaction in sperm (PubMed:15774481).By similarity1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi261Calcium 1By similarity1
Metal bindingi261Calcium 2By similarity1
Metal bindingi267Calcium 1By similarity1
Metal bindingi319Calcium 1By similarity1
Metal bindingi319Calcium 2By similarity1
Metal bindingi320Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi321Calcium 1By similarity1
Metal bindingi321Calcium 2By similarity1
Metal bindingi321Calcium 3By similarity1
Metal bindingi324Calcium 3By similarity1
Metal bindingi327Calcium 2By similarity1
Metal bindingi327Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: ParkinsonsUK-UCL
  • calcium-dependent protein binding Source: UniProtKB
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: MGI
  • phosphatidylserine binding Source: ParkinsonsUK-UCL
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • syntaxin binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-6
Alternative name(s):
Synaptotagmin VI
Short name:
SytVI
Gene namesi
Name:Syt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1859544. Syt6.

Subcellular locationi

  • Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass membrane protein 1 Publication
Isoform 1 :
  • Membrane; Single-pass membrane protein

  • Note: Localized predominantly to endoplasmic reticulum (ER) and/or Golgi-like perinuclear compartment (PubMed:10531344).1 Publication
Isoform 2 :
  • Cytoplasmcytosol 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 59VesicularSequence analysisAdd BLAST59
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 511CytoplasmicSequence analysisAdd BLAST431

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • exocytic vesicle Source: GO_Central
  • extrinsic component of membrane Source: UniProtKB
  • integral component of membrane Source: BHF-UCL
  • perinuclear endoplasmic reticulum Source: BHF-UCL
  • plasma membrane Source: UniProtKB
  • synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839551 – 511Synaptotagmin-6Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei217PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9R0N8.
PRIDEiQ9R0N8.

PTM databases

iPTMnetiQ9R0N8.
PhosphoSitePlusiQ9R0N8.

Expressioni

Tissue specificityi

Isoform 1 is expressed in the olfactory bulb. Isoform 2 is expressed in the brain (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027849.
CleanExiMM_SYT6.
ExpressionAtlasiQ9R0N8. baseline and differential.
GenevisibleiQ9R0N8. MM.

Interactioni

Subunit structurei

Isoform 1: Homodimer; disulfide-linked via the cysteine motif (PubMed:10531343, PubMed:10531344). Isoform 1: Can also form heterodimers with SYT3, SYT7, SYT9 and SYT10 (PubMed:10531343, PubMed:10531344, PubMed:10871604). Isoform 1: Interacts with STX1A, STX1B and STX2; the interaction is Ca2+-dependent (PubMed:15774481). Isoform 2: Is not able to form homodimer and heterodimers (PubMed:10531344).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-5239378,EBI-5239378
Syt10Q9R0N42EBI-5239378,EBI-5239459
Syt3O356812EBI-5239378,EBI-457995
Syt9Q9R0N92EBI-5239378,EBI-458006

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • clathrin binding Source: MGI
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • syntaxin binding Source: MGI

Protein-protein interaction databases

IntActiQ9R0N8. 3 interactors.
STRINGi10090.ENSMUSP00000088196.

Structurei

3D structure databases

ProteinModelPortaliQ9R0N8.
SMRiQ9R0N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini232 – 333C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini364 – 467C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 38Cysteine motifBy similarityAdd BLAST27
Regioni483 – 511Necessary for cell membrane association (isoform 2)1 PublicationAdd BLAST29

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT3F. Eukaryota.
ENOG410YBMV. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiQ9R0N8.
KOiK19906.
OMAiSTRHISF.
OrthoDBiEOG091G0XMQ.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028701. SYT6.
[Graphical view]
PANTHERiPTHR10024:SF45. PTHR10024:SF45. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0N8-1) [UniParc]FASTAAdd to basket
Also known as: SytVI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGVWGAGGP RCQAALAVLA SLCRARPPPL GLDVETCRSF ELQSPEQSPS
60 70 80 90 100
AADSGTSVSL LAVVVIVCGV ALVAVFLFLF WKLCWMPWRK KEASSPSSAN
110 120 130 140 150
PASETLQSPS SRGNMADKLK DPSALGFLEA AVKISHTSPD IPAEVQMSVK
160 170 180 190 200
EHIMRHTKLQ RQTTEPASST RHTSFKRHLP RQMHVSSVDY GNELPPAAAE
210 220 230 240 250
QPTSIGRIKP ELYKQKSVDG DDAKSEAAKS CGKINFSLRY DYESETLIVR
260 270 280 290 300
ILKAFDLPAK DFCGSSDPYV KIYLLPDRKC KLQTRVHRKT LNPTFDENFH
310 320 330 340 350
FPVPYEELAD RKLHLSVFDF DRFSRHDMIG EVILDNLFEA SDLSRETSIW
360 370 380 390 400
KDIQYATSES VDLGEIMFSL CYLPTAGRLT LTVIKCRNLK AMDITGYSDP
410 420 430 440 450
YVKVSLLCDG RRLKKKKTTI KKNTLNPIYN EAIIFDIPPE NMDQVSLLIS
460 470 480 490 500
VMDYDRVGHN EIIGVCRVGI NAEGLGRDHW NEMLAYPRKP IAHWHSLVEV
510
KKSFKEGTPR L
Length:511
Mass (Da):57,204
Last modified:July 27, 2011 - v2
Checksum:iC37C9305E3D05572
GO
Isoform 2 (identifier: Q9R0N8-2) [UniParc]FASTAAdd to basket
Also known as: SytVIdeltaTM1

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Show »
Length:426
Mass (Da):48,343
Checksum:i699B22C7B06C3179
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti428I → V in BAA85775 (PubMed:10531343).Curated1
Sequence conflicti428I → V in BAA85781 (PubMed:10531344).Curated1
Sequence conflicti428I → V in BAA85782 (PubMed:10531344).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0417291 – 85Missing in isoform 2. 1 PublicationAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026803 mRNA. Translation: BAA85775.1.
AB026809 mRNA. Translation: BAA85781.1.
AB026810 mRNA. Translation: BAA85782.1.
AC123057 Genomic DNA. No translation available.
CCDSiCCDS38575.1. [Q9R0N8-1]
CCDS71296.1. [Q9R0N8-2]
RefSeqiNP_061270.2. NM_018800.4. [Q9R0N8-1]
UniGeneiMm.488213.
Mm.88818.

Genome annotation databases

EnsembliENSMUST00000090697; ENSMUSP00000088196; ENSMUSG00000027849. [Q9R0N8-1]
ENSMUST00000117221; ENSMUSP00000113373; ENSMUSG00000027849. [Q9R0N8-2]
ENSMUST00000121834; ENSMUSP00000112997; ENSMUSG00000027849. [Q9R0N8-1]
GeneIDi54524.
KEGGimmu:54524.
UCSCiuc008qsy.2. mouse. [Q9R0N8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026803 mRNA. Translation: BAA85775.1.
AB026809 mRNA. Translation: BAA85781.1.
AB026810 mRNA. Translation: BAA85782.1.
AC123057 Genomic DNA. No translation available.
CCDSiCCDS38575.1. [Q9R0N8-1]
CCDS71296.1. [Q9R0N8-2]
RefSeqiNP_061270.2. NM_018800.4. [Q9R0N8-1]
UniGeneiMm.488213.
Mm.88818.

3D structure databases

ProteinModelPortaliQ9R0N8.
SMRiQ9R0N8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R0N8. 3 interactors.
STRINGi10090.ENSMUSP00000088196.

PTM databases

iPTMnetiQ9R0N8.
PhosphoSitePlusiQ9R0N8.

Proteomic databases

PaxDbiQ9R0N8.
PRIDEiQ9R0N8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090697; ENSMUSP00000088196; ENSMUSG00000027849. [Q9R0N8-1]
ENSMUST00000117221; ENSMUSP00000113373; ENSMUSG00000027849. [Q9R0N8-2]
ENSMUST00000121834; ENSMUSP00000112997; ENSMUSG00000027849. [Q9R0N8-1]
GeneIDi54524.
KEGGimmu:54524.
UCSCiuc008qsy.2. mouse. [Q9R0N8-1]

Organism-specific databases

CTDi148281.
MGIiMGI:1859544. Syt6.

Phylogenomic databases

eggNOGiENOG410IT3F. Eukaryota.
ENOG410YBMV. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiQ9R0N8.
KOiK19906.
OMAiSTRHISF.
OrthoDBiEOG091G0XMQ.
TreeFamiTF315600.

Miscellaneous databases

ChiTaRSiSyt6. mouse.
PROiQ9R0N8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027849.
CleanExiMM_SYT6.
ExpressionAtlasiQ9R0N8. baseline and differential.
GenevisibleiQ9R0N8. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028701. SYT6.
[Graphical view]
PANTHERiPTHR10024:SF45. PTHR10024:SF45. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYT6_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N8
Secondary accession number(s): E9QJV2, Q9QUK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.