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Q9R0N8

- SYT6_MOUSE

UniProt

Q9R0N8 - SYT6_MOUSE

Protein

Synaptotagmin-6

Gene

Syt6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis By similarity. May mediate Ca2+-regulation of exocytosis in acrosomal reaction in sperm.By similarity1 Publication

    Cofactori

    Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi261 – 2611Calcium 1By similarity
    Metal bindingi261 – 2611Calcium 2By similarity
    Metal bindingi267 – 2671Calcium 1By similarity
    Metal bindingi319 – 3191Calcium 1By similarity
    Metal bindingi319 – 3191Calcium 2By similarity
    Metal bindingi320 – 3201Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi321 – 3211Calcium 1By similarity
    Metal bindingi321 – 3211Calcium 2By similarity
    Metal bindingi321 – 3211Calcium 3By similarity
    Metal bindingi324 – 3241Calcium 3By similarity
    Metal bindingi327 – 3271Calcium 2By similarity
    Metal bindingi327 – 3271Calcium 3By similarity

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: UniProtKB
    2. identical protein binding Source: IntAct
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB
    5. protein heterodimerization activity Source: UniProtKB
    6. protein homodimerization activity Source: BHF-UCL
    7. transporter activity Source: InterPro

    GO - Biological processi

    1. acrosome reaction Source: HGNC

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptotagmin-6
    Alternative name(s):
    Synaptotagmin VI
    Short name:
    SytVI
    Gene namesi
    Name:Syt6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1859544. Syt6.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass membrane protein 1 Publication
    Isoform 1 : Membrane; Single-pass membrane protein
    Note: Localized predominantly to endoplasmic reticulum (ER) and/or Golgi-like perinuclear compartment.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytosol Source: UniProtKB
    3. extrinsic component of membrane Source: UniProtKB
    4. integral component of membrane Source: BHF-UCL
    5. perinuclear endoplasmic reticulum Source: BHF-UCL
    6. plasma membrane Source: UniProtKB
    7. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 511511Synaptotagmin-6PRO_0000183955Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei217 – 2171PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9R0N8.
    PRIDEiQ9R0N8.

    PTM databases

    PhosphoSiteiQ9R0N8.

    Expressioni

    Tissue specificityi

    Isoform 1 is expressed in the olfactory bulb. Isoform 2 is expressed in the brain (at protein level).

    Gene expression databases

    BgeeiQ9R0N8.
    CleanExiMM_SYT6.
    GenevestigatoriQ9R0N8.

    Interactioni

    Subunit structurei

    Homodimer (isoform 1). Isoform 1 forms heterodimers with SytIII, SytV and SytX. Interacts with STX1A, STX1B and STX2; the interaction is Ca2+-dependent. Isoform 2 is not able to form homodimer and heterodimers.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-5239378,EBI-5239378
    Syt10Q9R0N42EBI-5239378,EBI-5239459
    Syt3O356812EBI-5239378,EBI-457995
    Syt9Q9R0N92EBI-5239378,EBI-458006

    Protein-protein interaction databases

    IntActiQ9R0N8. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0N8.
    SMRiQ9R0N8. Positions 231-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5959VesicularSequence AnalysisAdd
    BLAST
    Topological domaini81 – 511431CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei60 – 8021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini232 – 333102C2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini364 – 467104C2 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni483 – 51129Necessary for cell membrane association (isoform 2)Add
    BLAST

    Sequence similaritiesi

    Belongs to the synaptotagmin family.Curated
    Contains 2 C2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5038.
    GeneTreeiENSGT00710000106556.
    HOGENOMiHOG000232128.
    HOVERGENiHBG005010.
    InParanoidiQ9QUK7.
    OMAiWRDIQYA.
    OrthoDBiEOG78PV8W.
    TreeFamiTF315600.

    Family and domain databases

    Gene3Di2.60.40.150. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR028701. SYT6.
    [Graphical view]
    PANTHERiPTHR10024:SF45. PTHR10024:SF45. 1 hit.
    PfamiPF00168. C2. 2 hits.
    [Graphical view]
    PRINTSiPR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTiSM00239. C2. 2 hits.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 2 hits.
    PROSITEiPS50004. C2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9R0N8-1) [UniParc]FASTAAdd to Basket

    Also known as: SytVI

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGVWGAGGP RCQAALAVLA SLCRARPPPL GLDVETCRSF ELQSPEQSPS    50
    AADSGTSVSL LAVVVIVCGV ALVAVFLFLF WKLCWMPWRK KEASSPSSAN 100
    PASETLQSPS SRGNMADKLK DPSALGFLEA AVKISHTSPD IPAEVQMSVK 150
    EHIMRHTKLQ RQTTEPASST RHTSFKRHLP RQMHVSSVDY GNELPPAAAE 200
    QPTSIGRIKP ELYKQKSVDG DDAKSEAAKS CGKINFSLRY DYESETLIVR 250
    ILKAFDLPAK DFCGSSDPYV KIYLLPDRKC KLQTRVHRKT LNPTFDENFH 300
    FPVPYEELAD RKLHLSVFDF DRFSRHDMIG EVILDNLFEA SDLSRETSIW 350
    KDIQYATSES VDLGEIMFSL CYLPTAGRLT LTVIKCRNLK AMDITGYSDP 400
    YVKVSLLCDG RRLKKKKTTI KKNTLNPIYN EAIIFDIPPE NMDQVSLLIS 450
    VMDYDRVGHN EIIGVCRVGI NAEGLGRDHW NEMLAYPRKP IAHWHSLVEV 500
    KKSFKEGTPR L 511
    Length:511
    Mass (Da):57,204
    Last modified:July 27, 2011 - v2
    Checksum:iC37C9305E3D05572
    GO
    Isoform 2 (identifier: Q9R0N8-2) [UniParc]FASTAAdd to Basket

    Also known as: SytVIdeltaTM1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-85: Missing.

    Show »
    Length:426
    Mass (Da):48,343
    Checksum:i699B22C7B06C3179
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti428 – 4281I → V in BAA85775. (PubMed:10531343)Curated
    Sequence conflicti428 – 4281I → V in BAA85781. (PubMed:10531344)Curated
    Sequence conflicti428 – 4281I → V in BAA85782. (PubMed:10531344)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8585Missing in isoform 2. 1 PublicationVSP_041729Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026803 mRNA. Translation: BAA85775.1.
    AB026809 mRNA. Translation: BAA85781.1.
    AB026810 mRNA. Translation: BAA85782.1.
    AC123057 Genomic DNA. No translation available.
    CCDSiCCDS38575.1. [Q9R0N8-1]
    CCDS71296.1. [Q9R0N8-2]
    RefSeqiNP_061270.2. NM_018800.4. [Q9R0N8-1]
    UniGeneiMm.488213.
    Mm.88818.

    Genome annotation databases

    EnsembliENSMUST00000090697; ENSMUSP00000088196; ENSMUSG00000027849. [Q9R0N8-1]
    ENSMUST00000117221; ENSMUSP00000113373; ENSMUSG00000027849. [Q9R0N8-2]
    ENSMUST00000121834; ENSMUSP00000112997; ENSMUSG00000027849. [Q9R0N8-1]
    GeneIDi54524.
    KEGGimmu:54524.
    UCSCiuc008qsy.1. mouse. [Q9R0N8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026803 mRNA. Translation: BAA85775.1 .
    AB026809 mRNA. Translation: BAA85781.1 .
    AB026810 mRNA. Translation: BAA85782.1 .
    AC123057 Genomic DNA. No translation available.
    CCDSi CCDS38575.1. [Q9R0N8-1 ]
    CCDS71296.1. [Q9R0N8-2 ]
    RefSeqi NP_061270.2. NM_018800.4. [Q9R0N8-1 ]
    UniGenei Mm.488213.
    Mm.88818.

    3D structure databases

    ProteinModelPortali Q9R0N8.
    SMRi Q9R0N8. Positions 231-501.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9R0N8. 3 interactions.

    PTM databases

    PhosphoSitei Q9R0N8.

    Proteomic databases

    PaxDbi Q9R0N8.
    PRIDEi Q9R0N8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000090697 ; ENSMUSP00000088196 ; ENSMUSG00000027849 . [Q9R0N8-1 ]
    ENSMUST00000117221 ; ENSMUSP00000113373 ; ENSMUSG00000027849 . [Q9R0N8-2 ]
    ENSMUST00000121834 ; ENSMUSP00000112997 ; ENSMUSG00000027849 . [Q9R0N8-1 ]
    GeneIDi 54524.
    KEGGi mmu:54524.
    UCSCi uc008qsy.1. mouse. [Q9R0N8-1 ]

    Organism-specific databases

    CTDi 148281.
    MGIi MGI:1859544. Syt6.

    Phylogenomic databases

    eggNOGi COG5038.
    GeneTreei ENSGT00710000106556.
    HOGENOMi HOG000232128.
    HOVERGENi HBG005010.
    InParanoidi Q9QUK7.
    OMAi WRDIQYA.
    OrthoDBi EOG78PV8W.
    TreeFami TF315600.

    Miscellaneous databases

    NextBioi 311360.
    PROi Q9R0N8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9R0N8.
    CleanExi MM_SYT6.
    Genevestigatori Q9R0N8.

    Family and domain databases

    Gene3Di 2.60.40.150. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR028701. SYT6.
    [Graphical view ]
    PANTHERi PTHR10024:SF45. PTHR10024:SF45. 1 hit.
    Pfami PF00168. C2. 2 hits.
    [Graphical view ]
    PRINTSi PR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTi SM00239. C2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 2 hits.
    PROSITEi PS50004. C2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
      Fukuda M., Kanno E., Mikoshiba K.
      J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: ICR.
      Tissue: Cerebellum.
    2. "A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
      Fukuda M., Mikoshiba K.
      J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION (ISOFORMS 1 AND 2), HOMODIMERIZATION, HETERODIMERIZATION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING.
      Strain: ICR.
      Tissue: Cerebellum.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction."
      Hutt D.M., Baltz J.M., Ngsee J.K.
      J. Biol. Chem. 280:20197-20203(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH STX1A; STX1B AND STX2.

    Entry informationi

    Entry nameiSYT6_MOUSE
    AccessioniPrimary (citable) accession number: Q9R0N8
    Secondary accession number(s): E9QJV2, Q9QUK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3