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Q9R0N5 (SYT5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptotagmin-5
Alternative name(s):
Synaptotagmin IX
Synaptotagmin V
Short name=SytV
Gene names
Name:Syt5
Synonyms:Syt9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca2+-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. Ref.5

Cofactor

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Subunit structure

Homodimer. Can also form heterodimers with SYT6. Interacts with both alpha- and beta-tubulin. Ref.4

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass membrane protein. Recycling endosome membrane; Single-pass membrane protein. Note: In mast cells, localizes to the endocytic recycling compartment. Ref.5

Sequence similarities

Belongs to the synaptotagmin family.

Contains 2 C2 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 386386Synaptotagmin-5
PRO_0000183952

Regions

Topological domain1 – 2424Vesicular Potential
Transmembrane25 – 4521Helical; Potential
Topological domain46 – 386341Cytoplasmic Potential
Domain124 – 21188C2 1
Domain253 – 34492C2 2

Sites

Metal binding1381Calcium 2; via carbonyl oxygen By similarity
Metal binding1391Calcium 1 By similarity
Metal binding1391Calcium 2 By similarity
Metal binding1451Calcium 1 By similarity
Metal binding1971Calcium 1 By similarity
Metal binding1971Calcium 2 By similarity
Metal binding1981Calcium 1; via carbonyl oxygen By similarity
Metal binding1991Calcium 1 By similarity
Metal binding1991Calcium 2 By similarity
Metal binding1991Calcium 3 By similarity
Metal binding2021Calcium 3 By similarity
Metal binding2051Calcium 2 By similarity
Metal binding2051Calcium 3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9R0N5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: F884902E032082F6

FASTA38643,130
        10         20         30         40         50         60 
MFPEPPTLGS PAPKTPPDSS RIRQGAVPAW VLATIVLGSG LLVFSSCFCL YRKRCRRRMG 

        70         80         90        100        110        120 
KKSQAQAQVH LQEVKELGRS YIDKVQPEIE ELDRSPSMPG QQVSDKHQLG RLQYSLDYDF 

       130        140        150        160        170        180 
QTGQLLVGIL QAQGLAALDL GGSSDPYVSV YLLPDKRRRH ETKVHRQTLN PHFGETFAFK 

       190        200        210        220        230        240 
VPYVELGGRV LVMAVYDFDR FSRNDAIGEV RVPMSSVNLG RPVQAWRELQ VAPKEEQEKL 

       250        260        270        280        290        300 
GDICFSLRYV PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN 

       310        320        330        340        350        360 
TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGAAVG GAGLRHWADM 

       370        380 
LANPRRPIAQ WHSLRPPDRA RPIPAP 

« Hide

References

« Hide 'large scale' references
[1]"Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
Fukuda M., Kanno E., Mikoshiba K.
J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
Tissue: Cerebellum.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[3]Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 57-61; 240-248; 256-264 AND 269-280, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
[4]"A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
Fukuda M., Mikoshiba K.
J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: HETERODIMERIZATION.
Strain: ICR.
Tissue: Cerebellum.
[5]"Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells."
Fukuda M., Kowalchyk J.A., Zhang X., Martin T.F.J., Mikoshiba K.
J. Biol. Chem. 277:4601-4604(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026806 mRNA. Translation: BAA85778.1.
BC047148 mRNA. Translation: AAH47148.1.
CCDSCCDS20738.1.
RefSeqNP_058604.1. NM_016908.2.
XP_006540252.1. XM_006540189.1.
XP_006540253.1. XM_006540190.1.
UniGeneMm.358663.

3D structure databases

ProteinModelPortalQ9R0N5.
SMRQ9R0N5. Positions 108-375.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207314. 1 interaction.
IntActQ9R0N5. 1 interaction.
MINTMINT-4998411.
STRING10090.ENSMUSP00000070322.

PTM databases

PhosphoSiteQ9R0N5.

Proteomic databases

PaxDbQ9R0N5.
PRIDEQ9R0N5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000065957; ENSMUSP00000070322; ENSMUSG00000004961.
GeneID53420.
KEGGmmu:53420.
UCSCuc009exy.1. mouse.

Organism-specific databases

CTD6861.
MGIMGI:1926368. Syt5.

Phylogenomic databases

eggNOGNOG292488.
GeneTreeENSGT00620000087641.
HOGENOMHOG000232127.
HOVERGENHBG005010.
InParanoidQ9R0N5.
OMANFAFKVP.
OrthoDBEOG78PV8W.
PhylomeDBQ9R0N5.
TreeFamTF315600.

Enzyme and pathway databases

ReactomeREACT_188937. Metabolism.

Gene expression databases

BgeeQ9R0N5.
CleanExMM_SYT5.
MM_SYT9.
GenevestigatorQ9R0N5.

Family and domain databases

Gene3D2.60.40.150. 2 hits.
InterProIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028689. SYT5.
[Graphical view]
PANTHERPTHR10024:SF37. PTHR10024:SF37. 1 hit.
PfamPF00168. C2. 2 hits.
[Graphical view]
PRINTSPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTSM00239. C2. 2 hits.
[Graphical view]
SUPFAMSSF49562. SSF49562. 2 hits.
PROSITEPS50004. C2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio310253.
PROQ9R0N5.
SOURCESearch...

Entry information

Entry nameSYT5_MOUSE
AccessionPrimary (citable) accession number: Q9R0N5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot