Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Synaptotagmin-5

Gene

Syt5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca2+-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi139 – 1391Calcium 1By similarity
Metal bindingi139 – 1391Calcium 2By similarity
Metal bindingi145 – 1451Calcium 1By similarity
Metal bindingi197 – 1971Calcium 1By similarity
Metal bindingi197 – 1971Calcium 2By similarity
Metal bindingi198 – 1981Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi199 – 1991Calcium 1By similarity
Metal bindingi199 – 1991Calcium 2By similarity
Metal bindingi199 – 1991Calcium 3By similarity
Metal bindingi202 – 2021Calcium 3By similarity
Metal bindingi205 – 2051Calcium 2By similarity
Metal bindingi205 – 2051Calcium 3By similarity

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: Ensembl
  2. metal ion binding Source: UniProtKB-KW
  3. protein heterodimerization activity Source: UniProtKB
  4. transporter activity Source: InterPro

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_263148. Regulation of insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-5
Alternative name(s):
Synaptotagmin IX
Synaptotagmin V
Short name:
SytV
Gene namesi
Name:Syt5
Synonyms:Syt9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1926368. Syt5.

Subcellular locationi

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass membrane protein 1 Publication. Recycling endosome membrane 1 Publication; Single-pass membrane protein 1 Publication
Note: In mast cells, localizes to the endocytic recycling compartment.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424VesicularSequence AnalysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence AnalysisAdd
BLAST
Topological domaini46 – 386341CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. dense core granule Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. neuronal cell body Source: MGI
  5. neuron projection Source: MGI
  6. perinuclear region of cytoplasm Source: MGI
  7. plasma membrane Source: Reactome
  8. recycling endosome membrane Source: UniProtKB-SubCell
  9. synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endosome, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Synaptotagmin-5PRO_0000183952Add
BLAST

Proteomic databases

MaxQBiQ9R0N5.
PaxDbiQ9R0N5.
PRIDEiQ9R0N5.

PTM databases

PhosphoSiteiQ9R0N5.

Expressioni

Gene expression databases

BgeeiQ9R0N5.
CleanExiMM_SYT5.
MM_SYT9.
GenevestigatoriQ9R0N5.

Interactioni

Subunit structurei

Homodimer. Can also form heterodimers with SYT6. Interacts with both alpha- and beta-tubulin.

Protein-protein interaction databases

BioGridi207314. 1 interaction.
IntActiQ9R0N5. 1 interaction.
MINTiMINT-4998411.
STRINGi10090.ENSMUSP00000070322.

Structurei

3D structure databases

ProteinModelPortaliQ9R0N5.
SMRiQ9R0N5. Positions 108-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 21188C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini253 – 34492C2 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292488.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiQ9R0N5.
OMAiMFSEPPT.
OrthoDBiEOG78PV8W.
PhylomeDBiQ9R0N5.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028689. SYT5.
[Graphical view]
PANTHERiPTHR10024:SF37. PTHR10024:SF37. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0N5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFPEPPTLGS PAPKTPPDSS RIRQGAVPAW VLATIVLGSG LLVFSSCFCL
60 70 80 90 100
YRKRCRRRMG KKSQAQAQVH LQEVKELGRS YIDKVQPEIE ELDRSPSMPG
110 120 130 140 150
QQVSDKHQLG RLQYSLDYDF QTGQLLVGIL QAQGLAALDL GGSSDPYVSV
160 170 180 190 200
YLLPDKRRRH ETKVHRQTLN PHFGETFAFK VPYVELGGRV LVMAVYDFDR
210 220 230 240 250
FSRNDAIGEV RVPMSSVNLG RPVQAWRELQ VAPKEEQEKL GDICFSLRYV
260 270 280 290 300
PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN
310 320 330 340 350
TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGAAVG
360 370 380
GAGLRHWADM LANPRRPIAQ WHSLRPPDRA RPIPAP
Length:386
Mass (Da):43,130
Last modified:May 1, 2000 - v1
Checksum:iF884902E032082F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026806 mRNA. Translation: BAA85778.1.
BC047148 mRNA. Translation: AAH47148.1.
CCDSiCCDS20738.1.
RefSeqiNP_058604.1. NM_016908.2.
XP_006540252.1. XM_006540189.1.
XP_006540253.1. XM_006540190.1.
UniGeneiMm.358663.

Genome annotation databases

EnsembliENSMUST00000065957; ENSMUSP00000070322; ENSMUSG00000004961.
GeneIDi53420.
KEGGimmu:53420.
UCSCiuc009exy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026806 mRNA. Translation: BAA85778.1.
BC047148 mRNA. Translation: AAH47148.1.
CCDSiCCDS20738.1.
RefSeqiNP_058604.1. NM_016908.2.
XP_006540252.1. XM_006540189.1.
XP_006540253.1. XM_006540190.1.
UniGeneiMm.358663.

3D structure databases

ProteinModelPortaliQ9R0N5.
SMRiQ9R0N5. Positions 108-375.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207314. 1 interaction.
IntActiQ9R0N5. 1 interaction.
MINTiMINT-4998411.
STRINGi10090.ENSMUSP00000070322.

PTM databases

PhosphoSiteiQ9R0N5.

Proteomic databases

MaxQBiQ9R0N5.
PaxDbiQ9R0N5.
PRIDEiQ9R0N5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065957; ENSMUSP00000070322; ENSMUSG00000004961.
GeneIDi53420.
KEGGimmu:53420.
UCSCiuc009exy.1. mouse.

Organism-specific databases

CTDi6861.
MGIiMGI:1926368. Syt5.

Phylogenomic databases

eggNOGiNOG292488.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiQ9R0N5.
OMAiMFSEPPT.
OrthoDBiEOG78PV8W.
PhylomeDBiQ9R0N5.
TreeFamiTF315600.

Enzyme and pathway databases

ReactomeiREACT_263148. Regulation of insulin secretion.

Miscellaneous databases

NextBioi310253.
PROiQ9R0N5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0N5.
CleanExiMM_SYT5.
MM_SYT9.
GenevestigatoriQ9R0N5.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028689. SYT5.
[Graphical view]
PANTHERiPTHR10024:SF37. PTHR10024:SF37. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
    Fukuda M., Kanno E., Mikoshiba K.
    J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 57-61; 240-248; 256-264 AND 269-280, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  4. "A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
    Fukuda M., Mikoshiba K.
    J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: HETERODIMERIZATION.
    Strain: ICR.
    Tissue: Cerebellum.
  5. "Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells."
    Fukuda M., Kowalchyk J.A., Zhang X., Martin T.F.J., Mikoshiba K.
    J. Biol. Chem. 277:4601-4604(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSYT5_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.