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Q9R0N5

- SYT5_MOUSE

UniProt

Q9R0N5 - SYT5_MOUSE

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Protein

Synaptotagmin-5

Gene

Syt5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca2+-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface.1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi139 – 1391Calcium 1By similarity
Metal bindingi139 – 1391Calcium 2By similarity
Metal bindingi145 – 1451Calcium 1By similarity
Metal bindingi197 – 1971Calcium 1By similarity
Metal bindingi197 – 1971Calcium 2By similarity
Metal bindingi198 – 1981Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi199 – 1991Calcium 1By similarity
Metal bindingi199 – 1991Calcium 2By similarity
Metal bindingi199 – 1991Calcium 3By similarity
Metal bindingi202 – 2021Calcium 3By similarity
Metal bindingi205 – 2051Calcium 2By similarity
Metal bindingi205 – 2051Calcium 3By similarity

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: Ensembl
  2. metal ion binding Source: UniProtKB-KW
  3. protein heterodimerization activity Source: UniProtKB
  4. transporter activity Source: InterPro

GO - Biological processi

  1. calcium ion-dependent exocytosis Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_263148. Regulation of insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-5
Alternative name(s):
Synaptotagmin IX
Synaptotagmin V
Short name:
SytV
Gene namesi
Name:Syt5
Synonyms:Syt9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1926368. Syt5.

Subcellular locationi

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass membrane protein 1 Publication. Recycling endosome membrane 1 Publication; Single-pass membrane protein 1 Publication
Note: In mast cells, localizes to the endocytic recycling compartment.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424VesicularSequence AnalysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence AnalysisAdd
BLAST
Topological domaini46 – 386341CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. dense core granule Source: MGI
  3. endosome Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. neuronal cell body Source: MGI
  6. neuron projection Source: MGI
  7. perinuclear region of cytoplasm Source: MGI
  8. plasma membrane Source: Reactome
  9. synaptic vesicle Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Endosome, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Synaptotagmin-5PRO_0000183952Add
BLAST

Proteomic databases

MaxQBiQ9R0N5.
PaxDbiQ9R0N5.
PRIDEiQ9R0N5.

PTM databases

PhosphoSiteiQ9R0N5.

Expressioni

Gene expression databases

BgeeiQ9R0N5.
CleanExiMM_SYT5.
MM_SYT9.
GenevestigatoriQ9R0N5.

Interactioni

Subunit structurei

Homodimer. Can also form heterodimers with SYT6. Interacts with both alpha- and beta-tubulin.

Protein-protein interaction databases

BioGridi207314. 1 interaction.
IntActiQ9R0N5. 1 interaction.
MINTiMINT-4998411.
STRINGi10090.ENSMUSP00000070322.

Structurei

3D structure databases

ProteinModelPortaliQ9R0N5.
SMRiQ9R0N5. Positions 108-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 21188C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini253 – 34492C2 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG292488.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiQ9R0N5.
OMAiNFAFKVP.
OrthoDBiEOG78PV8W.
PhylomeDBiQ9R0N5.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028689. SYT5.
[Graphical view]
PANTHERiPTHR10024:SF37. PTHR10024:SF37. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0N5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFPEPPTLGS PAPKTPPDSS RIRQGAVPAW VLATIVLGSG LLVFSSCFCL
60 70 80 90 100
YRKRCRRRMG KKSQAQAQVH LQEVKELGRS YIDKVQPEIE ELDRSPSMPG
110 120 130 140 150
QQVSDKHQLG RLQYSLDYDF QTGQLLVGIL QAQGLAALDL GGSSDPYVSV
160 170 180 190 200
YLLPDKRRRH ETKVHRQTLN PHFGETFAFK VPYVELGGRV LVMAVYDFDR
210 220 230 240 250
FSRNDAIGEV RVPMSSVNLG RPVQAWRELQ VAPKEEQEKL GDICFSLRYV
260 270 280 290 300
PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN
310 320 330 340 350
TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGAAVG
360 370 380
GAGLRHWADM LANPRRPIAQ WHSLRPPDRA RPIPAP
Length:386
Mass (Da):43,130
Last modified:May 1, 2000 - v1
Checksum:iF884902E032082F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026806 mRNA. Translation: BAA85778.1.
BC047148 mRNA. Translation: AAH47148.1.
CCDSiCCDS20738.1.
RefSeqiNP_058604.1. NM_016908.2.
XP_006540252.1. XM_006540189.1.
XP_006540253.1. XM_006540190.1.
UniGeneiMm.358663.

Genome annotation databases

EnsembliENSMUST00000065957; ENSMUSP00000070322; ENSMUSG00000004961.
GeneIDi53420.
KEGGimmu:53420.
UCSCiuc009exy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026806 mRNA. Translation: BAA85778.1 .
BC047148 mRNA. Translation: AAH47148.1 .
CCDSi CCDS20738.1.
RefSeqi NP_058604.1. NM_016908.2.
XP_006540252.1. XM_006540189.1.
XP_006540253.1. XM_006540190.1.
UniGenei Mm.358663.

3D structure databases

ProteinModelPortali Q9R0N5.
SMRi Q9R0N5. Positions 108-375.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207314. 1 interaction.
IntActi Q9R0N5. 1 interaction.
MINTi MINT-4998411.
STRINGi 10090.ENSMUSP00000070322.

PTM databases

PhosphoSitei Q9R0N5.

Proteomic databases

MaxQBi Q9R0N5.
PaxDbi Q9R0N5.
PRIDEi Q9R0N5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000065957 ; ENSMUSP00000070322 ; ENSMUSG00000004961 .
GeneIDi 53420.
KEGGi mmu:53420.
UCSCi uc009exy.1. mouse.

Organism-specific databases

CTDi 6861.
MGIi MGI:1926368. Syt5.

Phylogenomic databases

eggNOGi NOG292488.
GeneTreei ENSGT00760000118973.
HOGENOMi HOG000232127.
HOVERGENi HBG005010.
InParanoidi Q9R0N5.
OMAi NFAFKVP.
OrthoDBi EOG78PV8W.
PhylomeDBi Q9R0N5.
TreeFami TF315600.

Enzyme and pathway databases

Reactomei REACT_263148. Regulation of insulin secretion.

Miscellaneous databases

NextBioi 310253.
PROi Q9R0N5.
SOURCEi Search...

Gene expression databases

Bgeei Q9R0N5.
CleanExi MM_SYT5.
MM_SYT9.
Genevestigatori Q9R0N5.

Family and domain databases

Gene3Di 2.60.40.150. 2 hits.
InterProi IPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028689. SYT5.
[Graphical view ]
PANTHERi PTHR10024:SF37. PTHR10024:SF37. 1 hit.
Pfami PF00168. C2. 2 hits.
[Graphical view ]
PRINTSi PR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTi SM00239. C2. 2 hits.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 2 hits.
PROSITEi PS50004. C2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
    Fukuda M., Kanno E., Mikoshiba K.
    J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 57-61; 240-248; 256-264 AND 269-280, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.
  4. "A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
    Fukuda M., Mikoshiba K.
    J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: HETERODIMERIZATION.
    Strain: ICR.
    Tissue: Cerebellum.
  5. "Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells."
    Fukuda M., Kowalchyk J.A., Zhang X., Martin T.F.J., Mikoshiba K.
    J. Biol. Chem. 277:4601-4604(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSYT5_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3