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Q9R0N5

- SYT5_MOUSE

UniProt

Q9R0N5 - SYT5_MOUSE

Protein

Synaptotagmin-5

Gene

Syt5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca2+-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface.1 Publication

    Cofactori

    Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi138 – 1381Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi139 – 1391Calcium 1By similarity
    Metal bindingi139 – 1391Calcium 2By similarity
    Metal bindingi145 – 1451Calcium 1By similarity
    Metal bindingi197 – 1971Calcium 1By similarity
    Metal bindingi197 – 1971Calcium 2By similarity
    Metal bindingi198 – 1981Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi199 – 1991Calcium 1By similarity
    Metal bindingi199 – 1991Calcium 2By similarity
    Metal bindingi199 – 1991Calcium 3By similarity
    Metal bindingi202 – 2021Calcium 3By similarity
    Metal bindingi205 – 2051Calcium 2By similarity
    Metal bindingi205 – 2051Calcium 3By similarity

    GO - Molecular functioni

    1. calcium-dependent phospholipid binding Source: Ensembl
    2. metal ion binding Source: UniProtKB-KW
    3. protein heterodimerization activity Source: UniProtKB
    4. transporter activity Source: InterPro

    GO - Biological processi

    1. calcium ion-dependent exocytosis Source: InterPro

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptotagmin-5
    Alternative name(s):
    Synaptotagmin IX
    Synaptotagmin V
    Short name:
    SytV
    Gene namesi
    Name:Syt5
    Synonyms:Syt9
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1926368. Syt5.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 1 Publication; Single-pass membrane protein 1 Publication. Recycling endosome membrane 1 Publication; Single-pass membrane protein 1 Publication
    Note: In mast cells, localizes to the endocytic recycling compartment.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. dense core granule Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. neuronal cell body Source: MGI
    5. neuron projection Source: MGI
    6. perinuclear region of cytoplasm Source: MGI
    7. plasma membrane Source: Reactome
    8. recycling endosome membrane Source: UniProtKB-SubCell
    9. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Endosome, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 386386Synaptotagmin-5PRO_0000183952Add
    BLAST

    Proteomic databases

    PaxDbiQ9R0N5.
    PRIDEiQ9R0N5.

    PTM databases

    PhosphoSiteiQ9R0N5.

    Expressioni

    Gene expression databases

    BgeeiQ9R0N5.
    CleanExiMM_SYT5.
    MM_SYT9.
    GenevestigatoriQ9R0N5.

    Interactioni

    Subunit structurei

    Homodimer. Can also form heterodimers with SYT6. Interacts with both alpha- and beta-tubulin.

    Protein-protein interaction databases

    BioGridi207314. 1 interaction.
    IntActiQ9R0N5. 1 interaction.
    MINTiMINT-4998411.
    STRINGi10090.ENSMUSP00000070322.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0N5.
    SMRiQ9R0N5. Positions 108-375.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424VesicularSequence AnalysisAdd
    BLAST
    Topological domaini46 – 386341CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini124 – 21188C2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini253 – 34492C2 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the synaptotagmin family.Curated
    Contains 2 C2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG292488.
    GeneTreeiENSGT00620000087641.
    HOGENOMiHOG000232127.
    HOVERGENiHBG005010.
    InParanoidiQ9R0N5.
    OMAiNFAFKVP.
    OrthoDBiEOG78PV8W.
    PhylomeDBiQ9R0N5.
    TreeFamiTF315600.

    Family and domain databases

    Gene3Di2.60.40.150. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR028689. SYT5.
    [Graphical view]
    PANTHERiPTHR10024:SF37. PTHR10024:SF37. 1 hit.
    PfamiPF00168. C2. 2 hits.
    [Graphical view]
    PRINTSiPR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTiSM00239. C2. 2 hits.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 2 hits.
    PROSITEiPS50004. C2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R0N5-1 [UniParc]FASTAAdd to Basket

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    MFPEPPTLGS PAPKTPPDSS RIRQGAVPAW VLATIVLGSG LLVFSSCFCL    50
    YRKRCRRRMG KKSQAQAQVH LQEVKELGRS YIDKVQPEIE ELDRSPSMPG 100
    QQVSDKHQLG RLQYSLDYDF QTGQLLVGIL QAQGLAALDL GGSSDPYVSV 150
    YLLPDKRRRH ETKVHRQTLN PHFGETFAFK VPYVELGGRV LVMAVYDFDR 200
    FSRNDAIGEV RVPMSSVNLG RPVQAWRELQ VAPKEEQEKL GDICFSLRYV 250
    PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN 300
    TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGAAVG 350
    GAGLRHWADM LANPRRPIAQ WHSLRPPDRA RPIPAP 386
    Length:386
    Mass (Da):43,130
    Last modified:May 1, 2000 - v1
    Checksum:iF884902E032082F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026806 mRNA. Translation: BAA85778.1.
    BC047148 mRNA. Translation: AAH47148.1.
    CCDSiCCDS20738.1.
    RefSeqiNP_058604.1. NM_016908.2.
    XP_006540252.1. XM_006540189.1.
    XP_006540253.1. XM_006540190.1.
    UniGeneiMm.358663.

    Genome annotation databases

    EnsembliENSMUST00000065957; ENSMUSP00000070322; ENSMUSG00000004961.
    GeneIDi53420.
    KEGGimmu:53420.
    UCSCiuc009exy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026806 mRNA. Translation: BAA85778.1 .
    BC047148 mRNA. Translation: AAH47148.1 .
    CCDSi CCDS20738.1.
    RefSeqi NP_058604.1. NM_016908.2.
    XP_006540252.1. XM_006540189.1.
    XP_006540253.1. XM_006540190.1.
    UniGenei Mm.358663.

    3D structure databases

    ProteinModelPortali Q9R0N5.
    SMRi Q9R0N5. Positions 108-375.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207314. 1 interaction.
    IntActi Q9R0N5. 1 interaction.
    MINTi MINT-4998411.
    STRINGi 10090.ENSMUSP00000070322.

    PTM databases

    PhosphoSitei Q9R0N5.

    Proteomic databases

    PaxDbi Q9R0N5.
    PRIDEi Q9R0N5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000065957 ; ENSMUSP00000070322 ; ENSMUSG00000004961 .
    GeneIDi 53420.
    KEGGi mmu:53420.
    UCSCi uc009exy.1. mouse.

    Organism-specific databases

    CTDi 6861.
    MGIi MGI:1926368. Syt5.

    Phylogenomic databases

    eggNOGi NOG292488.
    GeneTreei ENSGT00620000087641.
    HOGENOMi HOG000232127.
    HOVERGENi HBG005010.
    InParanoidi Q9R0N5.
    OMAi NFAFKVP.
    OrthoDBi EOG78PV8W.
    PhylomeDBi Q9R0N5.
    TreeFami TF315600.

    Miscellaneous databases

    NextBioi 310253.
    PROi Q9R0N5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9R0N5.
    CleanExi MM_SYT5.
    MM_SYT9.
    Genevestigatori Q9R0N5.

    Family and domain databases

    Gene3Di 2.60.40.150. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR028689. SYT5.
    [Graphical view ]
    PANTHERi PTHR10024:SF37. PTHR10024:SF37. 1 hit.
    Pfami PF00168. C2. 2 hits.
    [Graphical view ]
    PRINTSi PR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTi SM00239. C2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 2 hits.
    PROSITEi PS50004. C2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
      Fukuda M., Kanno E., Mikoshiba K.
      J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
      Tissue: Cerebellum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 57-61; 240-248; 256-264 AND 269-280, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    4. "A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
      Fukuda M., Mikoshiba K.
      J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: HETERODIMERIZATION.
      Strain: ICR.
      Tissue: Cerebellum.
    5. "Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells."
      Fukuda M., Kowalchyk J.A., Zhang X., Martin T.F.J., Mikoshiba K.
      J. Biol. Chem. 277:4601-4604(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSYT5_MOUSE
    AccessioniPrimary (citable) accession number: Q9R0N5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3