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Protein

Galactokinase

Gene

Galk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major enzyme for galactose metabolism.

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei37 – 371Transition state stabilizerBy similarity
Active sitei186 – 1861Proton acceptorBy similarity
Binding sitei236 – 2361SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 14411ATPSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • galactitol metabolic process Source: MGI
  • galactose catabolic process via UDP-galactose Source: MGI
  • galactose metabolic process Source: MGI
  • glycolytic process from galactose Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-70370. Galactose catabolism.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactokinase (EC:2.7.1.6)
Alternative name(s):
Galactose kinase
Gene namesi
Name:Galk1
Synonyms:Galk, Glk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95730. Galk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392GalactokinasePRO_0000184646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9R0N0.
MaxQBiQ9R0N0.
PaxDbiQ9R0N0.
PRIDEiQ9R0N0.

PTM databases

iPTMnetiQ9R0N0.
PhosphoSiteiQ9R0N0.
SwissPalmiQ9R0N0.

Expressioni

Gene expression databases

CleanExiMM_GALK1.
GenevisibleiQ9R0N0. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi199945. 1 interaction.
IntActiQ9R0N0. 3 interactions.
MINTiMINT-1869525.
STRINGi10090.ENSMUSP00000021114.

Structurei

3D structure databases

ProteinModelPortaliQ9R0N0.
SMRiQ9R0N0. Positions 2-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 464Substrate bindingBy similarity
Regioni183 – 1864Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.Curated

Phylogenomic databases

eggNOGiKOG0631. Eukaryota.
COG0153. LUCA.
GeneTreeiENSGT00530000063433.
HOVERGENiHBG051695.
InParanoidiQ9R0N0.
KOiK00849.
OMAiANMPCGI.
OrthoDBiEOG7K3TMV.
PhylomeDBiQ9R0N0.
TreeFamiTF354326.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF6. PTHR10457:SF6. 1 hit.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0N0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAWRPPRVE ELLAEARRAF MEEFGAEPEL AVSAPGRVNL IGEHTDYNQG
60 70 80 90 100
LVLPMALELV TVMVGSPRTD GLVSLLTTSK DADEPQRLQF PLPSAQWSLE
110 120 130 140 150
PGIPQWANYV KGVIQHYPAS PLVGFSAVVV SSVPLGGGLS SSASLEVATY
160 170 180 190 200
TFIQQLCPDS GAIAARAQVC QRAEHSFAGV PCGIMDQLIA LLGQKGYALL
210 220 230 240 250
IDCRSLETSL VPLSDPKLAV LITNSNVRHS LGSSEYPVRR RQCEEVAQAL
260 270 280 290 300
GKESLREVRM EELEAGRELM SKEGFRRARH VVSEIRRTAQ AAAAMSRGDY
310 320 330 340 350
KAFGRLMVES HYSLRDDYEV SCPELDQLVE AALSVPGVYG SRMTGGGFGG
360 370 380 390
CTVTLLEASV APLVIDHIQE QYSGTATFYL SQAADGAQVL SL
Length:392
Mass (Da):42,295
Last modified:September 18, 2013 - v2
Checksum:i068432AD97CDDDFE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 202AF → V in BAA84705 (PubMed:10915771).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246459 Genomic DNA. Translation: AAF78226.1.
AB027012 mRNA. Translation: BAA84705.1.
AK045694 mRNA. Translation: BAC32460.1.
AK168716 mRNA. Translation: BAE40558.1.
AL607108 Genomic DNA. No translation available.
CH466558 Genomic DNA. Translation: EDL34544.1.
BC016602 mRNA. Translation: AAH16602.1.
CCDSiCCDS25654.1.
RefSeqiNP_058601.2. NM_016905.2.
UniGeneiMm.2820.

Genome annotation databases

EnsembliENSMUST00000021114; ENSMUSP00000021114; ENSMUSG00000020766.
GeneIDi14635.
KEGGimmu:14635.
UCSCiuc007mjn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246459 Genomic DNA. Translation: AAF78226.1.
AB027012 mRNA. Translation: BAA84705.1.
AK045694 mRNA. Translation: BAC32460.1.
AK168716 mRNA. Translation: BAE40558.1.
AL607108 Genomic DNA. No translation available.
CH466558 Genomic DNA. Translation: EDL34544.1.
BC016602 mRNA. Translation: AAH16602.1.
CCDSiCCDS25654.1.
RefSeqiNP_058601.2. NM_016905.2.
UniGeneiMm.2820.

3D structure databases

ProteinModelPortaliQ9R0N0.
SMRiQ9R0N0. Positions 2-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199945. 1 interaction.
IntActiQ9R0N0. 3 interactions.
MINTiMINT-1869525.
STRINGi10090.ENSMUSP00000021114.

PTM databases

iPTMnetiQ9R0N0.
PhosphoSiteiQ9R0N0.
SwissPalmiQ9R0N0.

Proteomic databases

EPDiQ9R0N0.
MaxQBiQ9R0N0.
PaxDbiQ9R0N0.
PRIDEiQ9R0N0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021114; ENSMUSP00000021114; ENSMUSG00000020766.
GeneIDi14635.
KEGGimmu:14635.
UCSCiuc007mjn.1. mouse.

Organism-specific databases

CTDi2584.
MGIiMGI:95730. Galk1.

Phylogenomic databases

eggNOGiKOG0631. Eukaryota.
COG0153. LUCA.
GeneTreeiENSGT00530000063433.
HOVERGENiHBG051695.
InParanoidiQ9R0N0.
KOiK00849.
OMAiANMPCGI.
OrthoDBiEOG7K3TMV.
PhylomeDBiQ9R0N0.
TreeFamiTF354326.

Enzyme and pathway databases

UniPathwayiUPA00214.
ReactomeiR-MMU-70370. Galactose catabolism.

Miscellaneous databases

ChiTaRSiGalk1. mouse.
PROiQ9R0N0.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GALK1.
GenevisibleiQ9R0N0. MM.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
InterProiIPR000705. Galactokinase.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF6. PTHR10457:SF6. 1 hit.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Mouse galactokinase (galK)."
    Seki N., Hattori A., Hayashi A., Kozuma S., Muramatsu M., Saito T.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina and Heart.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiGALK1_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N0
Secondary accession number(s): Q9JIA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 18, 2013
Last modified: June 8, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.