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Protein

Thiamin pyrophosphokinase 1

Gene

Tpk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.2 Publications

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamin pyrophosphokinase 1 (Tpk1)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • thiamine binding Source: InterPro
  • thiamine diphosphokinase activity Source: MGI

GO - Biological processi

  • thiamine diphosphate biosynthetic process Source: GO_Central
  • thiamine metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-196819. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamin pyrophosphokinase 1 (EC:2.7.6.2)
Short name:
mTPK1
Alternative name(s):
Thiamine pyrophosphokinase 1
Gene namesi
Name:Tpk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1352500. Tpk1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726481 – 243Thiamin pyrophosphokinase 1Add BLAST243

Proteomic databases

EPDiQ9R0M5.
PaxDbiQ9R0M5.
PeptideAtlasiQ9R0M5.
PRIDEiQ9R0M5.

PTM databases

PhosphoSitePlusiQ9R0M5.

Expressioni

Tissue specificityi

Detected in kidney and liver, and at lower levels in heart, brain and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000029735.
CleanExiMM_TPK1.
GenevisibleiQ9R0M5. MM.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065631.

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi9 – 11Combined sources3
Beta strandi12 – 14Combined sources3
Beta strandi19 – 23Combined sources5
Helixi32 – 38Combined sources7
Beta strandi40 – 45Combined sources6
Helixi48 – 54Combined sources7
Helixi60 – 62Combined sources3
Beta strandi66 – 70Combined sources5
Beta strandi72 – 75Combined sources4
Helixi77 – 85Combined sources9
Beta strandi89 – 92Combined sources4
Helixi100 – 114Combined sources15
Beta strandi120 – 125Combined sources6
Beta strandi128 – 130Combined sources3
Helixi132 – 144Combined sources13
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Beta strandi160 – 165Combined sources6
Beta strandi167 – 173Combined sources7
Beta strandi179 – 186Combined sources8
Beta strandi192 – 202Combined sources11
Beta strandi205 – 210Combined sources6
Turni211 – 213Combined sources3
Beta strandi216 – 219Combined sources4
Beta strandi223 – 233Combined sources11
Beta strandi235 – 241Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG3X-ray1.90A/B1-243[»]
2F17X-ray2.50A/B1-243[»]
ProteinModelPortaliQ9R0M5.
SMRiQ9R0M5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9R0M5.

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153. Eukaryota.
COG1564. LUCA.
GeneTreeiENSGT00390000016016.
HOGENOMiHOG000180834.
HOVERGENiHBG003568.
InParanoidiQ9R0M5.
KOiK00949.
OMAiGMEGSWC.
OrthoDBiEOG091G0FS8.
PhylomeDBiQ9R0M5.
TreeFamiTF313224.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0M5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEHAFTPLEP LLPTGNLKYC LVVLNQPLDA RFRHLWKKAL LRACADGGAN
60 70 80 90 100
HLYDLTEGER ESFLPEFVSG DFDSIRPEVK EYYTKKGCDL ISTPDQDHTD
110 120 130 140 150
FTKCLQVLQR KIEEKELQVD VIVTLGGLGG RFDQIMASVN TLFQATHITP
160 170 180 190 200
VPIIIIQKDS LIYLLQPGKH RLHVDTGMEG SWCGLIPVGQ PCNQVTTTGL
210 220 230 240
KWNLTNDVLG FGTLVSTSNT YDGSGLVTVE TDHPLLWTMA IKS
Length:243
Mass (Da):27,068
Last modified:May 1, 2000 - v1
Checksum:i946896D2493F44EA
GO
Isoform 2 (identifier: Q9R0M5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     119-167: Missing.

Show »
Length:194
Mass (Da):21,766
Checksum:i58E61181F8A33A9B
GO
Isoform 3 (identifier: Q9R0M5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-63: ALLRACADGGANHLYDLTEGERESF → VLGKKSQEVLAERRLIEPLGIQSSL
     64-243: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:63
Mass (Da):7,237
Checksum:i1AEFBAE8B507EC14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20C → W in BAC25948 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00959539 – 63ALLRA…ERESF → VLGKKSQEVLAERRLIEPLG IQSSL in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_00959664 – 243Missing in isoform 3. 1 PublicationAdd BLAST180
Alternative sequenceiVSP_009597119 – 167Missing in isoform 2. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027568 mRNA. Translation: BAA87040.1.
AK028431 mRNA. Translation: BAC25948.1.
AK035044 mRNA. Translation: BAC28923.1.
AK039131 mRNA. Translation: BAC30248.1.
AK136486 mRNA. Translation: BAE23001.1.
BC015246 mRNA. Translation: AAH15246.1.
BC023354 mRNA. Translation: AAH23354.1.
CCDSiCCDS39474.1. [Q9R0M5-1]
CCDS80529.1. [Q9R0M5-2]
RefSeqiNP_001298040.1. NM_001311111.1. [Q9R0M5-2]
NP_038889.1. NM_013861.4. [Q9R0M5-1]
UniGeneiMm.320979.

Genome annotation databases

EnsembliENSMUST00000067888; ENSMUSP00000065631; ENSMUSG00000029735. [Q9R0M5-1]
ENSMUST00000114644; ENSMUSP00000110291; ENSMUSG00000029735. [Q9R0M5-2]
GeneIDi29807.
KEGGimmu:29807.
UCSCiuc009bsq.1. mouse. [Q9R0M5-1]
uc009bsr.1. mouse. [Q9R0M5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027568 mRNA. Translation: BAA87040.1.
AK028431 mRNA. Translation: BAC25948.1.
AK035044 mRNA. Translation: BAC28923.1.
AK039131 mRNA. Translation: BAC30248.1.
AK136486 mRNA. Translation: BAE23001.1.
BC015246 mRNA. Translation: AAH15246.1.
BC023354 mRNA. Translation: AAH23354.1.
CCDSiCCDS39474.1. [Q9R0M5-1]
CCDS80529.1. [Q9R0M5-2]
RefSeqiNP_001298040.1. NM_001311111.1. [Q9R0M5-2]
NP_038889.1. NM_013861.4. [Q9R0M5-1]
UniGeneiMm.320979.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG3X-ray1.90A/B1-243[»]
2F17X-ray2.50A/B1-243[»]
ProteinModelPortaliQ9R0M5.
SMRiQ9R0M5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065631.

PTM databases

PhosphoSitePlusiQ9R0M5.

Proteomic databases

EPDiQ9R0M5.
PaxDbiQ9R0M5.
PeptideAtlasiQ9R0M5.
PRIDEiQ9R0M5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067888; ENSMUSP00000065631; ENSMUSG00000029735. [Q9R0M5-1]
ENSMUST00000114644; ENSMUSP00000110291; ENSMUSG00000029735. [Q9R0M5-2]
GeneIDi29807.
KEGGimmu:29807.
UCSCiuc009bsq.1. mouse. [Q9R0M5-1]
uc009bsr.1. mouse. [Q9R0M5-2]

Organism-specific databases

CTDi27010.
MGIiMGI:1352500. Tpk1.

Phylogenomic databases

eggNOGiKOG3153. Eukaryota.
COG1564. LUCA.
GeneTreeiENSGT00390000016016.
HOGENOMiHOG000180834.
HOVERGENiHBG003568.
InParanoidiQ9R0M5.
KOiK00949.
OMAiGMEGSWC.
OrthoDBiEOG091G0FS8.
PhylomeDBiQ9R0M5.
TreeFamiTF313224.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00597.
ReactomeiR-MMU-196819. Vitamin B1 (thiamin) metabolism.

Miscellaneous databases

EvolutionaryTraceiQ9R0M5.
PROiQ9R0M5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029735.
CleanExiMM_TPK1.
GenevisibleiQ9R0M5. MM.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTPK1_MOUSE
AccessioniPrimary (citable) accession number: Q9R0M5
Secondary accession number(s): Q3UWB5
, Q8CAB5, Q8CEE8, Q8R1Q6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.