Q9R0L7 (AKP8L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A-kinase anchor protein 8-like
Short name=AKAP8-like protein

Alternative name(s):
Neighbor of A-kinase-anchoring protein 95
Short name=Neighbor of AKAP95

Gene names

Name:	Akap8l
Synonyms:	Nakap, Nakap95

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta By similarity.
Subunit structure	Binds to the C-terminal of RNA helicase A and to TMPO-beta. Interacts with RNF43 By similarity. Interacts with PRPF40A. Ref.3
Subcellular location	Nucleus matrix. Cytoplasm By similarity. Note: Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix By similarity. Colocalizes with PRPF40A in the nuclear matrix. Ref.3
Post-translational modification	Phosphorylated on serine or threonine residues possibly by PKA By similarity.
Sequence similarities	Belongs to the AKAP95 family. Contains 2 C2H2 AKAP95-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear matrix Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	A-kinase anchor protein 8-like		PRO_0000075385
Regions
	Zinc finger	390 – 414	25	C2H2 AKAP95-type 1
	Zinc finger	483 – 508	26	C2H2 AKAP95-type 2
	Motif	275 – 280	6	Nuclear localization signal Potential
	Motif	363 – 365	3	Nuclear localization signal Potential
	Compositional bias	41 – 52	12	Gly/Tyr-rich
	Compositional bias	587 – 595	9	Pro-rich
	Compositional bias	600 – 606	7	Pro-rich
Amino acid modifications
	Modified residue	258	1	N6-acetyllysine By similarity
	Modified residue	284	1	Phosphoserine By similarity
	Modified residue	293	1	Phosphothreonine By similarity
	Modified residue	298	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9R0L7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 4CE8DFE66810A0FC	FASTA	642	71,454
	10 20 30 40 50 60 MSYTGFVQGS ETTLQSTYCD TSAQPTCDYG YGTWNSGTNR GYENYGYGYG YGQDNTTNYG 70 80 90 100 110 120 YGMATSHSWE MASSDTNANP SASGSASADS VLSRINQRLD MMPHLETDMI QGGVYGSGGG 130 140 150 160 170 180 ERYDSYEACD SRAILSERDL YRSSYDYGEL DPEMEMAYEG QYDAYRDQFR MRGGDTFGPR 190 200 210 220 230 240 AQGWARDARS GRPMASGYGR MWEDPMGARG QCMPGASRLP SLFSQNIIPE YGMFQGMRGG 250 260 270 280 290 300 GAFSGGSRFG FGFGNGMKQM RRTWKTWTTA DFRTKKKKRK QGGSPDEPDS KATRTDCSDN 310 320 330 340 350 360 SDSDNDEGTE GEAAEGTESA EAMEKGSRVD GEDEEGKEDG REEGKEDPEK GALTAQDESS 370 380 390 400 410 420 QAKRKLQASK KSQDKQKKRQ RDRMVERIQF VCSLCKYRTF YEDEMGSHLD SKFHKEHFKY 430 440 450 460 470 480 VGTKLPKQTA DFLQEYVTNK TKKTEELRKT VEDLDGLIQQ IYRDQDLTQE IAMEHFVKKV 490 500 510 520 530 540 EAAHCAACDL FIPMQFGIIQ KHLKTMDHNR NRRLMMEQSK KSSLMVARSI LNNKLISKKL 550 560 570 580 590 600 ERYLKGENPF TDNPEEEKEQ DEVEAGALDE GAPSEATELT EGVPAQPPVP LEPAPGTTTP 610 620 630 640 PPPPPPEEEE SPVPLLGGAL QCQIRGIPGL DMEDDEEGGG GP

References

[1]	"Mouse NAKAP95." Hattori A., Seki N., Hayashi A., Kozuma S., Muramatsu M.-A., Miyajima N., Saito T. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 511-520, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[3]	"Interaction of the nuclear matrix protein NAKAP with HypA and huntingtin: implications for nuclear toxicity in Huntington's disease pathogenesis." Sayer J.A., Manczak M., Akileswaran L., Reddy P.H., Coghlan V.M. NeuroMolecular Med. 7:297-310(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PRPF40A, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028921 mRNA. Translation: BAA84711.1.
IPI	IPI00127766.
UniGene	Mm.281005. Mm.488380.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9R0L7. 1 interaction.
STRING	10090.ENSMUSP00000051389.
PTM databases
PhosphoSite	Q9R0L7.
Proteomic databases
PaxDb	Q9R0L7.
PRIDE	Q9R0L7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:1860606. Akap8l.
Phylogenomic databases
eggNOG	NOG47695.
HOGENOM	HOG000169111.
HOVERGEN	HBG104297.
InParanoid	Q9R0L7.
OrthoDB	EOG434W6D.
Gene expression databases
CleanEx	MM_AKAP8L.
Genevestigator	Q9R0L7.
GermOnline	ENSMUSG00000002625. Mus musculus.
Family and domain databases
InterPro	IPR007071. AKAP95. IPR015880. Znf_C2H2-like. [Graphical view]
PANTHER	PTHR12190. PTHR12190. 1 hit.
Pfam	PF04988. AKAP95. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

AKP8L_MOUSE

Accession

Primary (citable) accession number: Q9R0L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program