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Protein

Pericentriolar material 1 protein

Gene

Pcm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins probably including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Probably involved in the biogenesis of cilia.1 Publication

GO - Biological processi

  • centrosome organization Source: MGI
  • cilium assembly Source: UniProtKB
  • cytoplasmic microtubule organization Source: MGI
  • interkinetic nuclear migration Source: BHF-UCL
  • intraciliary transport involved in cilium morphogenesis Source: UniProtKB
  • microtubule anchoring Source: BHF-UCL
  • microtubule anchoring at centrosome Source: MGI
  • negative regulation of neurogenesis Source: BHF-UCL
  • neuronal stem cell population maintenance Source: MGI
  • neuron migration Source: MGI
  • positive regulation of intracellular protein transport Source: UniProtKB
  • protein localization to centrosome Source: BHF-UCL
  • social behavior Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Pericentriolar material 1 protein
Short name:
PCM-1
Short name:
mPCM-1
Gene namesi
Name:Pcm1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1277958. Pcm1.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: MGI
  • centriolar satellite Source: BHF-UCL
  • centriole Source: MGI
  • centrosome Source: BHF-UCL
  • ciliary basal body Source: MGI
  • ciliary transition zone Source: MGI
  • cytoplasm Source: BHF-UCL
  • membrane Source: MGI
  • nuclear membrane Source: MGI
  • pericentriolar material Source: BHF-UCL
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002740382 – 2025Pericentriolar material 1 proteinAdd BLAST2024

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei65PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei93PhosphoserineBy similarity1
Modified residuei110PhosphoserineCombined sources1
Modified residuei116PhosphoserineCombined sources1
Modified residuei119PhosphoserineCombined sources1
Modified residuei159PhosphoserineBy similarity1
Modified residuei370PhosphoserineBy similarity1
Modified residuei372PhosphoserineBy similarity1
Modified residuei384PhosphoserineBy similarity1
Modified residuei399N6-acetyllysineBy similarity1
Modified residuei593PhosphoserineBy similarity1
Modified residuei644PhosphoserineCombined sources1
Modified residuei857PhosphothreonineBy similarity1
Modified residuei859PhosphoserineCombined sources1
Modified residuei864PhosphoserineCombined sources1
Modified residuei867PhosphoserineCombined sources1
Modified residuei870PhosphoserineCombined sources1
Modified residuei875PhosphothreonineCombined sources1
Modified residuei957PhosphoserineBy similarity1
Modified residuei974PhosphoserineBy similarity1
Modified residuei985PhosphoserineBy similarity1
Modified residuei988PhosphoserineBy similarity1
Modified residuei1182PhosphoserineBy similarity1
Modified residuei1185PhosphoserineCombined sources1
Modified residuei1228PhosphoserineBy similarity1
Modified residuei1254PhosphoserineCombined sources1
Modified residuei1257PhosphoserineCombined sources1
Modified residuei1259PhosphoserineCombined sources1
Modified residuei1260PhosphoserineCombined sources1
Modified residuei1315PhosphoserineCombined sources1
Modified residuei1317PhosphoserineCombined sources1
Modified residuei1466PhosphothreonineBy similarity1
Modified residuei1571PhosphoserineCombined sources1
Modified residuei1695PhosphoserineBy similarity1
Modified residuei1729PhosphoserineCombined sources1
Modified residuei1766PhosphoserineCombined sources1
Modified residuei1769PhosphoserineCombined sources1
Modified residuei1777PhosphoserineCombined sources1
Modified residuei1783PhosphoserineCombined sources1
Modified residuei1959PhosphoserineBy similarity1
Modified residuei1978PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9R0L6.
PeptideAtlasiQ9R0L6.
PRIDEiQ9R0L6.

PTM databases

iPTMnetiQ9R0L6.
PhosphoSitePlusiQ9R0L6.

Expressioni

Tissue specificityi

Expressed in the hippocampus and dentate gyrus, the columnar epithelial cells of bronchioles, the olfactory epithelium, the pericardium and the inner segment of the retina.1 Publication

Developmental stagei

Maternally derived during fertilization. Expressed in the pericardium of the developing embryo and in the epidermal layer surrounding the digits.1 Publication

Gene expression databases

BgeeiENSMUSG00000031592.
CleanExiMM_PCM1.
GenevisibleiQ9R0L6. MM.

Interactioni

Subunit structurei

Self-associates. Interacts with BBS4, BBS8, CETN3, HAP1, NDE1, NDEL1, MAP1LC3B, GABARAPAL2, and GABARAP. Interacts with CEP131; the interaction increases in response to ultraviolet light (UV) radiation. Associates with microtubule; association to microtubule is reduced in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling (By similarity). Interacts with C2CD3. Interacts with CCDC113 (By similarity). Interacts with SSX2IP (PubMed:24356449). Interacts with CCDC13 (By similarity). Interacts with CEP290 (PubMed:17705300). Interacts with PARD6A (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cep290Q6A0784EBI-4284371,EBI-1811999

Protein-protein interaction databases

BioGridi202047. 18 interactors.
IntActiQ9R0L6. 19 interactors.
MINTiMINT-4130770.
STRINGi10090.ENSMUSP00000039709.

Structurei

3D structure databases

ProteinModelPortaliQ9R0L6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili218 – 301Sequence analysisAdd BLAST84
Coiled coili399 – 426Sequence analysisAdd BLAST28
Coiled coili492 – 518Sequence analysisAdd BLAST27
Coiled coili652 – 772Sequence analysisAdd BLAST121
Coiled coili822 – 856Sequence analysisAdd BLAST35
Coiled coili985 – 1017Sequence analysisAdd BLAST33
Coiled coili1061 – 1086Sequence analysisAdd BLAST26

Sequence similaritiesi

Belongs to the PCM1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEGR. Eukaryota.
ENOG410YNFX. LUCA.
GeneTreeiENSGT00390000006641.
HOVERGENiHBG053890.
InParanoidiQ9R0L6.
KOiK16537.
OMAiTWNEVNS.
OrthoDBiEOG091G00C3.
TreeFamiTF328740.

Family and domain databases

InterProiIPR031446. PCM1_C.
IPR024138. Pericentriolar_Pcm1.
[Graphical view]
PANTHERiPTHR14164. PTHR14164. 1 hit.
PfamiPF15717. PCM1_C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0L6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATGGGPFEE VMHDQDLPNW SNDSVDDRLN NMEWGGQQKK ANRSSEKNKK
60 70 80 90 100
KFGVASDKRV TNAISPESSP GVGRRRTKIP HTFPHSRYMT QMSVPEQAEL
110 120 130 140 150
EKLKQRINFS DLDQRSIGSD SQGRATAANN KRQLSENRKP FNFLPMQINT
160 170 180 190 200
NKSKDATASL PKREMTTSAQ CKELFASALS NDLLQNCQVS EEDGRGEPAM
210 220 230 240 250
ESSQIVSRLV QIRDYITKAS SMREDLVEKN ERSANVERLT HLIEHLKEQE
260 270 280 290 300
KSYMKFLQKI LARDPQQEPM EETENLKKQH DLLKRMLQQQ EQLRALQGRQ
310 320 330 340 350
AALLALQHKA EQAIAVMDDS VVTETTGSLS GVSITSELNE ELNDLIQRFH
360 370 380 390 400
NQLRDSQPPA VPDNRRQAES LSLTREISQS RNPSVSEHLP DEKVQLFSKM
410 420 430 440 450
RVLQEKKQKM DKLLGELHNL RDQHLNNSSF VPSTSLQRSG DKRSSTVALS
460 470 480 490 500
APVGFASAVN GEANSLISSV PCPATSLVSQ NESENEGHLN PAEKLQKLNE
510 520 530 540 550
VQKRLNELRE LVHYYEQTSD MMTDAVNENT KDEETEESEY DSEHENSEPV
560 570 580 590 600
TNIRNPQVAS TWNEVNTNSN TQCGSNNRDG RPVNSNCEIN NRSAANIRAL
610 620 630 640 650
NMPPLDCRYN REGEQRLHVA HGEDEEEEVE EEGVSGASLS SRRSSLVDEA
660 670 680 690 700
PEDEEFEQKI SRLMAAKEKL KQLQDLVAMV QDDDATQVVV PAASNLDDFY
710 720 730 740 750
AAEEDIKQNS NNARENSNKI DTGVNEKTRE KFYEAKLQQQ QRELKQLQEE
760 770 780 790 800
RKKLIEIQEK IQAVQKACPD LQLSATSISS GPTKKYLPAI TSTPTVNEND
810 820 830 840 850
SSTSKCVIDP EDSSVVDNEL WSDMRRHEML REELRQRRKQ LEALMAEHQR
860 870 880 890 900
RQGLAETSSP VAISLRSDGS ENLCTPQQSR TEKTMATWGG STQCALDEEG
910 920 930 940 950
DEDGYLSEGI VRTDEEEEEE QDASSNDNFP IYPPSMNQNS YNVKETKTRW
960 970 980 990 1000
KSNRPVSADG NYRPLAKTRQ QNISMQRQEN LRWVSELSYI EEKEQWQEQI
1010 1020 1030 1040 1050
NQLKKQLDFS VNICQTLMQD QQTLSCLLQT LLTGPYSVLP SNVASPQVHL
1060 1070 1080 1090 1100
IMHQLNQCYT QLTWQQNNVQ RLKQMLTELM RQQNQHPEKP RSKERGSSAS
1110 1120 1130 1140 1150
HPSSPNLFCP FSFPTQPVNL FNLPGFTNFP SFAPGMNFSP LFPSNFGDFS
1160 1170 1180 1190 1200
QNVSTPTEQQ QPLAQNPSGK TEYMAFPKPF ESSSSLGAEK QRNQKQPEEE
1210 1220 1230 1240 1250
AENTKTPWLY DQEGGVEKPF FKTGFTESVE KATNSNRKNQ PDTSRRRRQF
1260 1270 1280 1290 1300
DEESLESFSS MPDPIDPTTV TKTFKTRKAS AQASLASKDK TPKSKSKKRN
1310 1320 1330 1340 1350
STQLKSRVKN IGYESASVSS TCEPCKNRNR HSAQTEEPVQ AKLFSRKNHE
1360 1370 1380 1390 1400
QLEKIIKYSR SAEISSETGS DFSMFEALRD TIYSEVATLI SQNESRPHFL
1410 1420 1430 1440 1450
IELFHELQLL NTDYLRQRAL YALQDIVSRH ISESDEREGE NVKPVNSGTW
1460 1470 1480 1490 1500
VASNSELTPS ESLVTTDDET FEKNFERETH KVSEQNDADN VSVMSVSSNF
1510 1520 1530 1540 1550
EPFATDDLGN TVIHLDQALA RMREYERMKT ETESHSNMRC TCRVIEDEDG
1560 1570 1580 1590 1600
AAAAATVSNS EETPIIENHN SPQPISDVSA VPCPRIDTQQ LDRQIKAIMK
1610 1620 1630 1640 1650
EVIPFLKEHM DEVCSSQLLT SVRRMVLTLT QQNDESKEFV KFFHKQLGSI
1660 1670 1680 1690 1700
LQDSLAKFAG RKLKDCGEDL LVEISEVLFN ELAFFKLMQD LDNNSIAVKQ
1710 1720 1730 1740 1750
RCKRKIEAAG VRQSYAKEAK RILEGDHGSP AGEIDDEDKD KDETETVKQT
1760 1770 1780 1790 1800
QTSEVYDAKG PKNVRSDVSD QEEDEESERC PVSINLSKAE SQALTNYGSG
1810 1820 1830 1840 1850
EDENEDEEME DFEESPVDIQ TSLQANTETT EENEHDSQIL QHDLEKTPES
1860 1870 1880 1890 1900
TNVPSDQEPT SKNDQDSSPV KPCYLNILEN EQQLNSATHK DSLTTTDSSK
1910 1920 1930 1940 1950
QPEPLPLPLA ASETLVPRVK EVKSAQETPE SSLAGSPDTE SPVLVNDYEA
1960 1970 1980 1990 2000
ESGNISQKSD EEDFVKVEDL PLKLTVYSEE ELRKKMIEEE QKNHLSGEIC
2010 2020
EMQTEELAGN SQILKEPETV GAQSI
Length:2,025
Mass (Da):228,847
Last modified:July 27, 2011 - v2
Checksum:i39D790997E6C5418
GO
Isoform 2 (identifier: Q9R0L6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-263: R → RENEEEDVRTVDSAVGSGSVAESTSLNADVQSEASDTTAR

Show »
Length:2,064
Mass (Da):232,843
Checksum:i38384D030FEC47BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti305A → T in AAH53728 (PubMed:15489334).Curated1
Sequence conflicti408Q → R in AAH53728 (PubMed:15489334).Curated1
Sequence conflicti1216V → I in AAK39513 (Ref. 5) Curated1
Sequence conflicti1329N → S in AAK39513 (Ref. 5) Curated1
Sequence conflicti1819I → V in BAA87861 (PubMed:10579718).Curated1
Sequence conflicti1819I → V in AAK21980 (Ref. 5) Curated1
Sequence conflicti2018 – 2025ETVGAQSI → GKSYQF in BAC30604 (PubMed:16141072).Curated8

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022612263R → RENEEEDVRTVDSAVGSGSV AESTSLNADVQSEASDTTAR in isoform 2. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029291 mRNA. Translation: BAA87861.1.
AC144926 Genomic DNA. No translation available.
AC156554 Genomic DNA. No translation available.
BC053728 mRNA. Translation: AAH53728.1.
AK011147 mRNA. Translation: BAB27430.3.
AK040482 mRNA. Translation: BAC30604.1.
AK141516 mRNA. Translation: BAE24712.1.
AY028080 Genomic DNA. Translation: AAK21980.1.
AF352180 mRNA. Translation: AAK39513.1.
AF369838 mRNA. Translation: AAK39564.1.
AF369839 mRNA. Translation: AAK39565.1.
AF039021 Genomic DNA. Translation: AAC96068.1.
AK020493 mRNA. Translation: BAB32121.1.
CCDSiCCDS22261.1. [Q9R0L6-1]
RefSeqiNP_076151.2. NM_023662.3. [Q9R0L6-1]
XP_006509361.1. XM_006509298.2. [Q9R0L6-2]
UniGeneiMm.117896.

Genome annotation databases

EnsembliENSMUST00000045218; ENSMUSP00000039709; ENSMUSG00000031592. [Q9R0L6-1]
ENSMUST00000211247; ENSMUSP00000147887; ENSMUSG00000031592. [Q9R0L6-2]
GeneIDi18536.
KEGGimmu:18536.
UCSCiuc009lnr.2. mouse. [Q9R0L6-1]
uc009lns.1. mouse. [Q9R0L6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029291 mRNA. Translation: BAA87861.1.
AC144926 Genomic DNA. No translation available.
AC156554 Genomic DNA. No translation available.
BC053728 mRNA. Translation: AAH53728.1.
AK011147 mRNA. Translation: BAB27430.3.
AK040482 mRNA. Translation: BAC30604.1.
AK141516 mRNA. Translation: BAE24712.1.
AY028080 Genomic DNA. Translation: AAK21980.1.
AF352180 mRNA. Translation: AAK39513.1.
AF369838 mRNA. Translation: AAK39564.1.
AF369839 mRNA. Translation: AAK39565.1.
AF039021 Genomic DNA. Translation: AAC96068.1.
AK020493 mRNA. Translation: BAB32121.1.
CCDSiCCDS22261.1. [Q9R0L6-1]
RefSeqiNP_076151.2. NM_023662.3. [Q9R0L6-1]
XP_006509361.1. XM_006509298.2. [Q9R0L6-2]
UniGeneiMm.117896.

3D structure databases

ProteinModelPortaliQ9R0L6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202047. 18 interactors.
IntActiQ9R0L6. 19 interactors.
MINTiMINT-4130770.
STRINGi10090.ENSMUSP00000039709.

PTM databases

iPTMnetiQ9R0L6.
PhosphoSitePlusiQ9R0L6.

Proteomic databases

PaxDbiQ9R0L6.
PeptideAtlasiQ9R0L6.
PRIDEiQ9R0L6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045218; ENSMUSP00000039709; ENSMUSG00000031592. [Q9R0L6-1]
ENSMUST00000211247; ENSMUSP00000147887; ENSMUSG00000031592. [Q9R0L6-2]
GeneIDi18536.
KEGGimmu:18536.
UCSCiuc009lnr.2. mouse. [Q9R0L6-1]
uc009lns.1. mouse. [Q9R0L6-2]

Organism-specific databases

CTDi5108.
MGIiMGI:1277958. Pcm1.

Phylogenomic databases

eggNOGiENOG410IEGR. Eukaryota.
ENOG410YNFX. LUCA.
GeneTreeiENSGT00390000006641.
HOVERGENiHBG053890.
InParanoidiQ9R0L6.
KOiK16537.
OMAiTWNEVNS.
OrthoDBiEOG091G00C3.
TreeFamiTF328740.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

ChiTaRSiPcm1. mouse.
PROiQ9R0L6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031592.
CleanExiMM_PCM1.
GenevisibleiQ9R0L6. MM.

Family and domain databases

InterProiIPR031446. PCM1_C.
IPR024138. Pericentriolar_Pcm1.
[Graphical view]
PANTHERiPTHR14164. PTHR14164. 1 hit.
PfamiPF15717. PCM1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCM1_MOUSE
AccessioniPrimary (citable) accession number: Q9R0L6
Secondary accession number(s): E9QLK2
, O70287, Q3URH6, Q7TMS7, Q8C9V2, Q91Y27, Q91Y28, Q91Y51, Q923L0, Q9CRK8, Q9CT57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.