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Protein

Cullin-associated NEDD8-dissociated protein 2

Gene

Cand2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes.By similarity

GO - Molecular functioni

  • TBP-class protein binding Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-associated NEDD8-dissociated protein 2
Alternative name(s):
Cullin-associated and neddylation-dissociated protein 2
TBP-interacting protein b
TBP-interacting protein of 120 kDa B
Short name:
TBP-interacting protein 120B
p120 CAND2
Gene namesi
Name:Cand2
Synonyms:Tip120b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620480. Cand2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 12731272Cullin-associated NEDD8-dissociated protein 2PRO_0000089299Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Post-translational modificationi

Ubiquitinated and targeted for proteasomal degradation.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PaxDbiQ9R0L4.
PRIDEiQ9R0L4.

Expressioni

Tissue specificityi

Detected in heart and skeletal muscle.

Interactioni

Subunit structurei

Binds TBP, CNOT3 and UBE3C.By similarity

GO - Molecular functioni

  • TBP-class protein binding Source: RGD

Protein-protein interaction databases

BioGridi251362. 1 interaction.
STRINGi10116.ENSRNOP00000014207.

Structurei

3D structure databases

ProteinModelPortaliQ9R0L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 3635HEAT 1Add
BLAST
Repeati37 – 7438HEAT 2Add
BLAST
Repeati82 – 11938HEAT 3Add
BLAST
Repeati121 – 15737HEAT 4Add
BLAST
Repeati167 – 20539HEAT 5Add
BLAST
Repeati209 – 24638HEAT 6Add
BLAST
Repeati248 – 28437HEAT 7Add
BLAST
Repeati292 – 32938HEAT 8Add
BLAST
Repeati364 – 40542HEAT 9Add
BLAST
Repeati409 – 44638HEAT 10Add
BLAST
Repeati469 – 50638HEAT 11Add
BLAST
Repeati554 – 59138HEAT 12Add
BLAST
Repeati602 – 64140HEAT 13Add
BLAST
Repeati685 – 72238HEAT 14Add
BLAST
Repeati727 – 76438HEAT 15Add
BLAST
Repeati768 – 80740HEAT 16Add
BLAST
Repeati809 – 85042HEAT 17Add
BLAST
Repeati894 – 93138HEAT 18Add
BLAST
Repeati933 – 96836HEAT 19Add
BLAST
Repeati970 – 100334HEAT 20Add
BLAST
Repeati1004 – 104037HEAT 21Add
BLAST
Repeati1044 – 108138HEAT 22Add
BLAST
Repeati1085 – 112137HEAT 23Add
BLAST
Repeati1142 – 117837HEAT 24Add
BLAST
Repeati1194 – 123138HEAT 25Add
BLAST
Repeati1241 – 127333HEAT 26Add
BLAST

Sequence similaritiesi

Belongs to the CAND family.Curated
Contains 26 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1824. Eukaryota.
ENOG410XPK4. LUCA.
HOGENOMiHOG000264713.
HOVERGENiHBG053467.
InParanoidiQ9R0L4.
PhylomeDBiQ9R0L4.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013932. TATA-bd_TIP120.
[Graphical view]
PfamiPF08623. TIP120. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9R0L4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTGAFYISS LLEKMTSSDK DFSPKSLGGS RVLDPLPWLQ ILAAITDWIS
60 70 80 90 100
GDRTQDLALP RFMATSDLMS ELQKDSIQLD EDSERKVVRT LLRLLEDRSG
110 120 130 140 150
EVQNLAVKCL GPLVGKVKEY QVENIVDTLC ANMRSDKEQL RDIAGIGLKT
160 170 180 190 200
VLSELPPAAT GSGLAISVCR KITGQLTSAI AQQEDVAVQL EALDILSDML
210 220 230 240 250
SRLGAPLGTF HASLLHCLLP QLSSPRLAVR KRTVVALGHL AAACSTDLFV
260 270 280 290 300
ELADHLVDRL PGPRAPASPA AIRTLIQCLG SVGRQAGHRL GAHLDRLMPL
310 320 330 340 350
VEEFCNLDDD ELRESCLQAF EAFLRKCPKE MDPHVPNVTS LCLQYMKHDP
360 370 380 390 400
NYNHDSDEEE QMETEDSEFS EQESEDEYSD DDDMSWKVRR AAAKCMAALI
410 420 430 440 450
SSRPDLLPDF HCTLAPALIR CFKEREENVK ADIFGAYIML LRHTRPPKGW
460 470 480 490 500
LEAVEEPTQT GRNLNMLRAQ VPLVMKALQR QLKDRNVRTR QGCFNLFTEL
510 520 530 540 550
AGVLPGCLAE HMTVLVSGIV FSLADYSSSS TIRMDALAFL QGLLGTEPAE
560 570 580 590 600
AFHPHLPTLL PPVMACVADP FYKVAAEALL VLQELVRTLW PLDRPRLLDP
610 620 630 640 650
EPYVGEMSTA TLARLRATDL DQEVKERAIS CVGHLVGHLG DRLGDDLEPT
660 670 680 690 700
LLLLLDRLRN EITRLPAVKA LTLVAVSPLR LDLQPILAEA LPILASFLRK
710 720 730 740 750
NQRALRLATL AALDALAQSQ GLGLPPPAVR SVLAELPALV SENDMHVAQL
760 770 780 790 800
AVDFLTTVTQ TQPASLVEVS GPVLEELLQL LHSPLLPAGV LAATEGFLQA
810 820 830 840 850
LVGTRPPCVE YSELISLLTA PVYNQVGDGG PGLHKQVFHS LARCVAALSA
860 870 880 890 900
ACPQEAAGTA SRLVCDARSP HSSTGVKVLA FLSLAEVGQV AGPGPQRELK
910 920 930 940 950
TVLLEALGSP SEDVRAAAAY ALGRVGAGNL PDFLPFLLAQ IEAQPRRQYL
960 970 980 990 1000
LLHALREALG AAQPDNLKPY VEDVWALLFQ RCESPEEGTR CVVAECIGKL
1010 1020 1030 1040 1050
VFVNPPFLLP RFRKQLAAGQ PYTRSTVITA VKFLISDQPH SIDPLLKSFI
1060 1070 1080 1090 1100
AEFMESLQDP DLNVRRATLT FFNSAVHNKP SLVRDLLDDI LPLLYQETKI
1110 1120 1130 1140 1150
RRDLIREVEM GPFKHTVDDG LDVRKAAFEC MYSLLESCLG QLDICEFLNH
1160 1170 1180 1190 1200
VEDGLKDHYD IRMLTFIMLA RLAALCPAPV LQRVDRLIEP LRATCTAKVK
1210 1220 1230 1240 1250
AGSVKQELEK QDELKRSAMR AVAALMTNPE VRKSPSVADF STQIRSNPEL
1260 1270
ATLFESIQKD TASGPSMDSM ELS
Length:1,273
Mass (Da):139,673
Last modified:May 1, 2000 - v1
Checksum:i71E81B9B3F242DB4
GO
Isoform 2 (identifier: Q9R0L4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     23-60: Missing.

Show »
Length:1,235
Mass (Da):135,590
Checksum:iF21384BFA1630549
GO
Isoform 3 (identifier: Q9R0L4-3) [UniParc]FASTAAdd to basket

Also known as: Short form

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Show »
Length:1,211
Mass (Da):132,846
Checksum:i2C19ED45D126B14F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6262Missing in isoform 3. 1 PublicationVSP_013951Add
BLAST
Alternative sequencei23 – 6038Missing in isoform 2. 1 PublicationVSP_013952Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029324 mRNA. Translation: BAA83619.1.
AB029341 mRNA. Translation: BAA83620.1.
AB029342 mRNA. Translation: BAA83621.1.
RefSeqiNP_852027.1. NM_181362.1. [Q9R0L4-2]
UniGeneiRn.118129.

Genome annotation databases

GeneIDi192226.
KEGGirno:192226.
UCSCiRGD:620480. rat. [Q9R0L4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029324 mRNA. Translation: BAA83619.1.
AB029341 mRNA. Translation: BAA83620.1.
AB029342 mRNA. Translation: BAA83621.1.
RefSeqiNP_852027.1. NM_181362.1. [Q9R0L4-2]
UniGeneiRn.118129.

3D structure databases

ProteinModelPortaliQ9R0L4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251362. 1 interaction.
STRINGi10116.ENSRNOP00000014207.

Proteomic databases

PaxDbiQ9R0L4.
PRIDEiQ9R0L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192226.
KEGGirno:192226.
UCSCiRGD:620480. rat. [Q9R0L4-1]

Organism-specific databases

CTDi23066.
RGDi620480. Cand2.

Phylogenomic databases

eggNOGiKOG1824. Eukaryota.
ENOG410XPK4. LUCA.
HOGENOMiHOG000264713.
HOVERGENiHBG053467.
InParanoidiQ9R0L4.
PhylomeDBiQ9R0L4.

Miscellaneous databases

NextBioi622832.
PROiQ9R0L4.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013932. TATA-bd_TIP120.
[Graphical view]
PfamiPF08623. TIP120. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "TIP120B: a novel TIP120-family protein that is expressed specifically in muscle tissues."
    Aoki T., Okada N., Ishida M., Yogosawa S., Makino Y., Tamura T.-A.
    Biochem. Biophys. Res. Commun. 261:911-916(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Skeletal muscle.
  2. "Proteolytic targeting of transcriptional regulator TIP120B by a HECT domain E3 ligase."
    You J., Wang M., Aoki T., Tamura T.-A., Pickart C.M.
    J. Biol. Chem. 278:23369-23375(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBE3C, UBIQUITINATION AND PROTEOLYTIC DEGRADATION.

Entry informationi

Entry nameiCAND2_RAT
AccessioniPrimary (citable) accession number: Q9R0L4
Secondary accession number(s): Q9R0L3, Q9R0L5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.