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Protein

Heat shock factor protein 4

Gene

Hsf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE). The HSF4A isoform represses transcription while the HSF4B isoform activates transcription.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi17 – 122106By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • camera-type eye development Source: MGI
  • cell development Source: MGI
  • eye development Source: MGI
  • histone H3-K9 demethylation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein homotrimerization Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 4
Short name:
HSF 4
Short name:
mHSF4
Alternative name(s):
Heat shock transcription factor 4
Short name:
HSTF 4
Gene namesi
Name:Hsf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1347058. Hsf4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Heat shock factor protein 4PRO_0000124572Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki293 – 293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei298 – 2981PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293 (By similarity).By similarity
Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9R0L1.
MaxQBiQ9R0L1.
PaxDbiQ9R0L1.
PRIDEiQ9R0L1.

PTM databases

iPTMnetiQ9R0L1.
PhosphoSiteiQ9R0L1.

Expressioni

Tissue specificityi

Preferentially expressed in brain and lung. Also found in the eye. Slightly detected in liver and skeletal muscle. Isoform B is the major species in various tissues.1 Publication

Gene expression databases

BgeeiQ9R0L1.
CleanExiMM_HSF4.
ExpressionAtlasiQ9R0L1. baseline and differential.
GenevisibleiQ9R0L1. MM.

Interactioni

Subunit structurei

Homotrimer. Exhibits constitutive DNA binding and forms trimers even in the absence of stress. Interacts with ALKBH4, DUSP26, MAPK1, MAPK2 and MAP kinase p38 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048904.

Structurei

3D structure databases

ProteinModelPortaliQ9R0L1.
SMRiQ9R0L1. Positions 13-124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 20375Hydrophobic repeat HR-A/BAdd
BLAST
Regioni245 – 32278Interactions with DUSP26, MAPK1 and MAPK2By similarityAdd
BLAST
Regioni364 – 38926Hydrophobic repeat HR-CAdd
BLAST

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ9R0L1.
KOiK09417.
OMAiVEFQHPS.
PhylomeDBiQ9R0L1.
TreeFamiTF330401.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027723. HSF4.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF43. PTHR10015:SF43. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform HSF4B (identifier: Q9R0L1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQEAPAALPT EPGPSPVPAF LGKLWALVGD PGTDHLIRWS PSGTSFLVSD
60 70 80 90 100
QSRFAKEVLP QYFKHSNMAS FVRQLNMYGF RKVVSIEQGG LLRPERDHVE
110 120 130 140 150
FQHPSFVRGR EQLLERVRRK VPALRGDDSR WRPEDLSRLL GEVQALRGVQ
160 170 180 190 200
ESTEARLQEL RQQNEILWRE VVTLRQSHSQ QHRVIGKLIQ CLFGPLQTGP
210 220 230 240 250
SSTGAKRKLS LMLDEGSACS ASAKFNACPV SGALLQDPYF IQSPLPETTL
260 270 280 290 300
GLSPHRARGP IISDIPEDSP SPEGHRLSPS GGCRRVKGLA LLKEEPASPG
310 320 330 340 350
GDGEAGLALA PNECDFCVTA PPPLPVAVVQ AILEGKGSYS PEGPRSVQQP
360 370 380 390 400
EPRGPREVPD RGTLGLDRGN RSPESLLPPM LLRPAPETLE PVAPVDVLGP
410 420 430 440 450
SLHGREWTLM DLDMELSLMQ PLAPETDEAE LTVKELNSSG VGKDHTLGTP
460 470 480 490
LMLDVQADLE GAALSVPGAL TLYNVTESNA SYLDPGASPS SP
Length:492
Mass (Da):53,255
Last modified:February 21, 2001 - v2
Checksum:i952AB78255AD3CA0
GO
Isoform HSF4A (identifier: Q9R0L1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     245-319: LPETTLGLSP...APNECDFCVT → SPCSPSQRPR...TGFLPPVVAG

Show »
Length:462
Mass (Da):50,239
Checksum:iD2237C6323F1376D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei245 – 31975LPETT…DFCVT → SPCSPSQRPRWASALTGPEG PSSLTSQKILHLLKDTGFLP PVVAG in isoform HSF4A. 1 PublicationVSP_002419Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029349 mRNA. Translation: BAA84583.1.
AB029350 mRNA. Translation: BAA84584.1.
CCDSiCCDS57638.1. [Q9R0L1-2]
CCDS57639.1. [Q9R0L1-1]
RefSeqiNP_001242971.1. NM_001256042.1. [Q9R0L1-1]
NP_036069.1. NM_011939.3. [Q9R0L1-2]
UniGeneiMm.89204.

Genome annotation databases

EnsembliENSMUST00000036127; ENSMUSP00000048904; ENSMUSG00000033249. [Q9R0L1-1]
ENSMUST00000173859; ENSMUSP00000134213; ENSMUSG00000033249. [Q9R0L1-2]
GeneIDi26386.
KEGGimmu:26386.
UCSCiuc009nca.2. mouse. [Q9R0L1-2]
uc009ncb.2. mouse. [Q9R0L1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029349 mRNA. Translation: BAA84583.1.
AB029350 mRNA. Translation: BAA84584.1.
CCDSiCCDS57638.1. [Q9R0L1-2]
CCDS57639.1. [Q9R0L1-1]
RefSeqiNP_001242971.1. NM_001256042.1. [Q9R0L1-1]
NP_036069.1. NM_011939.3. [Q9R0L1-2]
UniGeneiMm.89204.

3D structure databases

ProteinModelPortaliQ9R0L1.
SMRiQ9R0L1. Positions 13-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048904.

PTM databases

iPTMnetiQ9R0L1.
PhosphoSiteiQ9R0L1.

Proteomic databases

EPDiQ9R0L1.
MaxQBiQ9R0L1.
PaxDbiQ9R0L1.
PRIDEiQ9R0L1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036127; ENSMUSP00000048904; ENSMUSG00000033249. [Q9R0L1-1]
ENSMUST00000173859; ENSMUSP00000134213; ENSMUSG00000033249. [Q9R0L1-2]
GeneIDi26386.
KEGGimmu:26386.
UCSCiuc009nca.2. mouse. [Q9R0L1-2]
uc009ncb.2. mouse. [Q9R0L1-1]

Organism-specific databases

CTDi3299.
MGIiMGI:1347058. Hsf4.

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ9R0L1.
KOiK09417.
OMAiVEFQHPS.
PhylomeDBiQ9R0L1.
TreeFamiTF330401.

Miscellaneous databases

PROiQ9R0L1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0L1.
CleanExiMM_HSF4.
ExpressionAtlasiQ9R0L1. baseline and differential.
GenevisibleiQ9R0L1. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027723. HSF4.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF43. PTHR10015:SF43. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The mammalian HSF4 gene generates both an activator and a repressor of heat shock genes by alternative splicing."
    Tanabe M., Sasai N., Nagata K., Liu X.-D., Liu P.C.C., Thiele D.J., Nakai A.
    J. Biol. Chem. 274:27845-27856(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS HSF4A AND HSF4B), CHARACTERIZATION.
  2. "Association and regulation of heat shock transcription factor 4b with both extracellular signal-regulated kinase mitogen-activated protein kinase and dual-specificity tyrosine phosphatase DUSP26."
    Hu Y., Mivechi N.F.
    Mol. Cell. Biol. 26:3282-3294(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiHSF4_MOUSE
AccessioniPrimary (citable) accession number: Q9R0L1
Secondary accession number(s): Q9R0L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: June 8, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.