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Protein

Plasma membrane calcium-transporting ATPase 2

Gene

Atp2b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4544-aspartylphosphate intermediateBy similarity1
Metal bindingi775MagnesiumBy similarity1
Metal bindingi779MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • auditory receptor cell stereocilium organization Source: MGI
  • calcium ion transport Source: DFLAT
  • cell morphogenesis Source: MGI
  • cellular calcium ion homeostasis Source: MGI
  • cerebellar granule cell differentiation Source: MGI
  • cerebellar Purkinje cell differentiation Source: MGI
  • cerebellar Purkinje cell layer morphogenesis Source: MGI
  • cerebellum development Source: MGI
  • cGMP metabolic process Source: MGI
  • cochlea development Source: DFLAT
  • detection of mechanical stimulus involved in sensory perception of sound Source: MGI
  • inner ear development Source: MGI
  • inner ear morphogenesis Source: MGI
  • inner ear receptor cell differentiation Source: MGI
  • lactation Source: MGI
  • locomotion Source: MGI
  • locomotory behavior Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • neuron differentiation Source: UniProtKB
  • organelle organization Source: MGI
  • otolith mineralization Source: MGI
  • positive regulation of calcium ion transport Source: MGI
  • regulation of cell size Source: MGI
  • regulation of cytosolic calcium ion concentration Source: DFLAT
  • regulation of synaptic plasticity Source: MGI
  • sensory perception of sound Source: UniProtKB
  • serotonin metabolic process Source: MGI
  • synapse organization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.8. 3474.
ReactomeiR-MMU-418359. Reduction of cytosolic Ca++ levels.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 2 (EC:3.6.3.8)
Short name:
PMCA2
Alternative name(s):
Plasma membrane calcium ATPase isoform 2
Plasma membrane calcium pump isoform 2
Gene namesi
Name:Atp2b2
Synonyms:Pmca2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:105368. Atp2b2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 94CytoplasmicSequence analysisAdd BLAST94
Transmembranei95 – 115HelicalSequence analysisAdd BLAST21
Topological domaini116 – 152ExtracellularSequence analysisAdd BLAST37
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 345CytoplasmicSequence analysisAdd BLAST172
Transmembranei346 – 365HelicalSequence analysisAdd BLAST20
Topological domaini366 – 398ExtracellularSequence analysisAdd BLAST33
Transmembranei399 – 416HelicalSequence analysisAdd BLAST18
Topological domaini417 – 830CytoplasmicSequence analysisAdd BLAST414
Transmembranei831 – 850HelicalSequence analysisAdd BLAST20
Topological domaini851 – 860ExtracellularSequence analysis10
Transmembranei861 – 881HelicalSequence analysisAdd BLAST21
Topological domaini882 – 901CytoplasmicSequence analysisAdd BLAST20
Transmembranei902 – 924HelicalSequence analysisAdd BLAST23
Topological domaini925 – 942ExtracellularSequence analysisAdd BLAST18
Transmembranei943 – 964HelicalSequence analysisAdd BLAST22
Topological domaini965 – 983CytoplasmicSequence analysisAdd BLAST19
Transmembranei984 – 1005HelicalSequence analysisAdd BLAST22
Topological domaini1006 – 1015ExtracellularSequence analysis10
Transmembranei1016 – 1037HelicalSequence analysisAdd BLAST22
Topological domaini1038 – 1198CytoplasmicSequence analysisAdd BLAST161

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • cell junction Source: UniProtKB-KW
  • cilium Source: MGI
  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum Source: MGI
  • extracellular exosome Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • neuronal cell body Source: MGI
  • plasma membrane Source: UniProtKB
  • synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Atp2b2 null deficient mice are deaf.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462151 – 1198Plasma membrane calcium-transporting ATPase 2Add BLAST1198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineCombined sources1
Modified residuei319PhosphoserineBy similarity1
Modified residuei1094Phosphothreonine; by PKCBy similarity1
Modified residuei1133PhosphoserineCombined sources1
Modified residuei1143PhosphothreonineCombined sources1
Modified residuei1156PhosphoserineCombined sources1
Modified residuei1166PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9R0K7.
MaxQBiQ9R0K7.
PaxDbiQ9R0K7.
PRIDEiQ9R0K7.

PTM databases

iPTMnetiQ9R0K7.
PhosphoSitePlusiQ9R0K7.
SwissPalmiQ9R0K7.

Expressioni

Tissue specificityi

Expressed in the retina, with strongest levels in the inner plexiform layer, weaker levels in the outer plexiform layer, and very low levels in the proximal inner nuclear layer (PubMed:12209837). Specifically expressed in the following retinal cell types: rod bipolar cells, horizontal cells, amacrine cells and ganglion cells (PubMed:12209837). Also found in the cochlea (stereocilia and outer wall of hair cells) (at protein level) (PubMed:9697703). Strongly expressed in brain cortex. Found at low levels in heart, liver, lung and testis during late gestation (PubMed:9697703).2 Publications

Gene expression databases

BgeeiENSMUSG00000030302.
CleanExiMM_ATP2B2.
ExpressionAtlasiQ9R0K7. baseline and differential.
GenevisibleiQ9R0K7. MM.

Interactioni

Subunit structurei

Interacts with PDZD11.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198250. 2 interactors.
IntActiQ9R0K7. 5 interactors.
MINTiMINT-4088651.
STRINGi10090.ENSMUSP00000086398.

Structurei

3D structure databases

ProteinModelPortaliQ9R0K7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1078 – 1095Calmodulin-binding subdomain ABy similarityAdd BLAST18
Regioni1096 – 1105Calmodulin-binding subdomain BBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi294 – 297Poly-Glu4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ9R0K7.
KOiK05850.
TreeFamiTF300330.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0K7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMTNSDFY SKNQRNESSH GGEFGCTMEE LRSLMELRGT EAVVKIKETY
60 70 80 90 100
GDTEAICRRL KTSPVEGLPG TAPDLEKRKQ IFGQNFIPPK KPKTFLQLVW
110 120 130 140 150
EALQDVTLII LEIAAIISLG LSFYHPPGES NEGCATAQGG AEDEGEAEAG
160 170 180 190 200
WIEGAAILLS VICVVLVTAF NDWSKEKQFR GLQSRIEQEQ KFTVVRAGQV
210 220 230 240 250
VQIPVAEIVV GDIAQIKYGD LLPADGLFIQ GNDLKIDESS LTGESDQVRK
260 270 280 290 300
SVDKDPMLLS GTHVMEGSGR MVVTAVGVNS QTGIIFTLLG AGGEEEEKKD
310 320 330 340 350
KKAKQQDGAA AMEMQPLKSA EGGDADDKKK ANMHKKEKSV LQGKLTKLAV
360 370 380 390 400
QIGKAGLVMS AITVIILVLY FTVDTFVVNK KPWLTECTPV YVQYFVKFFI
410 420 430 440 450
IGVTVLVVAV PEGLPLAVTI SLAYSVKKMM KDNNLVRHLD ACETMGNATA
460 470 480 490 500
ICSDKTGTLT TNRMTVVQAY VGDVHYKEIP DPSSINAKTL ELLVNAIAIN
510 520 530 540 550
SAYTTKILPP EKEGALPRQV GNKTECGLLG FVLDLRQDYE PVRSQMPEEK
560 570 580 590 600
LYKVYTFNSV RKSMSTVIKM PDESFRMYSK GASEIVLKKC CKILSGAGEA
610 620 630 640 650
RVFRPRDRDE MVKKVIEPMA CDGLRTICVA YRDFPSSPEP DWDNENDILN
660 670 680 690 700
ELTCICVVGI EDPVRPEVPE AIRKCQRAGI TVRMVTGDNI NTARAIAIKC
710 720 730 740 750
GIIHPGEDFL CLEGKEFNRR IRNEKGEIEQ ERIDKIWPKL RVLARSSPTD
760 770 780 790 800
KHTLVKGIID STHTEQRQVV AVTGDGTNDG PALKKADVGF AMGIAGTDVA
810 820 830 840 850
KEASDIILTD DNFSSIVKAV MWGRNVYDSI SKFLQFQLTV NVVAVIVAFT
860 870 880 890 900
GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTETLL LRKPYGRNKP
910 920 930 940 950
LISRTMMKNI LGHAVYQLTL IFTLLFVGEK MFQIDSGRNA PLHSPPSEHY
960 970 980 990 1000
TIIFNTFVMM QLFNEINARK IHGERNVFDG IFRNPIFCTI VLGTFAIQIV
1010 1020 1030 1040 1050
IVQFGGKPFS CSPLQLDQWM WCIFIGLGEL VWGQVIATIP TSRLKFLKEA
1060 1070 1080 1090 1100
GRLTQKEEIP EEELNEDVEE IDHAERELRR GQILWFRGLN RIQTQIRVVK
1110 1120 1130 1140 1150
AFRSSLYEGL EKPESRTSIH NFMAHPEFRI EDSQPHIPLI DDTDLEEDAA
1160 1170 1180 1190
LKQNSSPPSS LNKNNSAIDS GINLTTDTSK SATSSSPGSP IHSLETSL
Length:1,198
Mass (Da):132,588
Last modified:June 1, 2001 - v2
Checksum:iBAA8CC28620D994B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti243G → S in dfw. 1 Publication1
Natural varianti412E → K.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053471 mRNA. Translation: AAC61255.1.
AB030737 mRNA. Translation: BAA83104.1.
AB030738 mRNA. Translation: BAA83105.1.
CCDSiCCDS20433.1.
RefSeqiNP_001031761.1. NM_001036684.3.
NP_033853.1. NM_009723.6.
XP_017176842.1. XM_017321353.1.
UniGeneiMm.321755.
Mm.443564.

Genome annotation databases

EnsembliENSMUST00000089003; ENSMUSP00000086398; ENSMUSG00000030302.
ENSMUST00000101045; ENSMUSP00000098606; ENSMUSG00000030302.
GeneIDi11941.
KEGGimmu:11941.
UCSCiuc009dhn.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053471 mRNA. Translation: AAC61255.1.
AB030737 mRNA. Translation: BAA83104.1.
AB030738 mRNA. Translation: BAA83105.1.
CCDSiCCDS20433.1.
RefSeqiNP_001031761.1. NM_001036684.3.
NP_033853.1. NM_009723.6.
XP_017176842.1. XM_017321353.1.
UniGeneiMm.321755.
Mm.443564.

3D structure databases

ProteinModelPortaliQ9R0K7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198250. 2 interactors.
IntActiQ9R0K7. 5 interactors.
MINTiMINT-4088651.
STRINGi10090.ENSMUSP00000086398.

PTM databases

iPTMnetiQ9R0K7.
PhosphoSitePlusiQ9R0K7.
SwissPalmiQ9R0K7.

Proteomic databases

EPDiQ9R0K7.
MaxQBiQ9R0K7.
PaxDbiQ9R0K7.
PRIDEiQ9R0K7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089003; ENSMUSP00000086398; ENSMUSG00000030302.
ENSMUST00000101045; ENSMUSP00000098606; ENSMUSG00000030302.
GeneIDi11941.
KEGGimmu:11941.
UCSCiuc009dhn.1. mouse.

Organism-specific databases

CTDi491.
MGIiMGI:105368. Atp2b2.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ9R0K7.
KOiK05850.
TreeFamiTF300330.

Enzyme and pathway databases

BRENDAi3.6.3.8. 3474.
ReactomeiR-MMU-418359. Reduction of cytosolic Ca++ levels.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Miscellaneous databases

ChiTaRSiAtp2b2. mouse.
PROiQ9R0K7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030302.
CleanExiMM_ATP2B2.
ExpressionAtlasiQ9R0K7. baseline and differential.
GenevisibleiQ9R0K7. MM.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT2B2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0K7
Secondary accession number(s): O88863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.