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Q9R0K7

- AT2B2_MOUSE

UniProt

Q9R0K7 - AT2B2_MOUSE

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Protein
Plasma membrane calcium-transporting ATPase 2
Gene
Atp2b2, Pmca2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei454 – 45414-aspartylphosphate intermediate By similarity
Metal bindingi775 – 7751Magnesium By similarity
Metal bindingi779 – 7791Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. PDZ domain binding Source: UniProtKB
  3. calcium ion binding Source: UniProtKB
  4. calcium-dependent ATPase activity Source: MGI
  5. calcium-transporting ATPase activity Source: UniProtKB
  6. calmodulin binding Source: UniProtKB
  7. protein C-terminus binding Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. auditory receptor cell stereocilium organization Source: MGI
  3. cGMP metabolic process Source: MGI
  4. calcium ion transport Source: DFLAT
  5. cell morphogenesis Source: MGI
  6. cellular calcium ion homeostasis Source: MGI
  7. cerebellar Purkinje cell differentiation Source: MGI
  8. cerebellar Purkinje cell layer morphogenesis Source: MGI
  9. cerebellar granule cell differentiation Source: MGI
  10. cerebellum development Source: MGI
  11. cochlea development Source: DFLAT
  12. cytosolic calcium ion homeostasis Source: DFLAT
  13. detection of mechanical stimulus involved in sensory perception of sound Source: MGI
  14. inner ear development Source: MGI
  15. inner ear morphogenesis Source: MGI
  16. inner ear receptor cell differentiation Source: MGI
  17. lactation Source: MGI
  18. locomotion Source: MGI
  19. locomotory behavior Source: MGI
  20. neuromuscular process controlling balance Source: MGI
  21. neuron differentiation Source: UniProtKB
  22. organelle organization Source: MGI
  23. otolith mineralization Source: MGI
  24. positive regulation of calcium ion transport Source: MGI
  25. regulation of cell size Source: MGI
  26. regulation of synaptic plasticity Source: MGI
  27. sensory perception of sound Source: UniProtKB
  28. serotonin metabolic process Source: MGI
  29. synapse organization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 2 (EC:3.6.3.8)
Short name:
PMCA2
Alternative name(s):
Plasma membrane calcium ATPase isoform 2
Plasma membrane calcium pump isoform 2
Gene namesi
Name:Atp2b2
Synonyms:Pmca2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:105368. Atp2b2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9494Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei95 – 11521Helical; Reviewed prediction
Add
BLAST
Topological domaini116 – 15237Extracellular Reviewed prediction
Add
BLAST
Transmembranei153 – 17321Helical; Reviewed prediction
Add
BLAST
Topological domaini174 – 345172Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei346 – 36520Helical; Reviewed prediction
Add
BLAST
Topological domaini366 – 39833Extracellular Reviewed prediction
Add
BLAST
Transmembranei399 – 41618Helical; Reviewed prediction
Add
BLAST
Topological domaini417 – 830414Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei831 – 85020Helical; Reviewed prediction
Add
BLAST
Topological domaini851 – 86010Extracellular Reviewed prediction
Transmembranei861 – 88121Helical; Reviewed prediction
Add
BLAST
Topological domaini882 – 90120Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei902 – 92423Helical; Reviewed prediction
Add
BLAST
Topological domaini925 – 94218Extracellular Reviewed prediction
Add
BLAST
Transmembranei943 – 96422Helical; Reviewed prediction
Add
BLAST
Topological domaini965 – 98319Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei984 – 100522Helical; Reviewed prediction
Add
BLAST
Topological domaini1006 – 101510Extracellular Reviewed prediction
Transmembranei1016 – 103722Helical; Reviewed prediction
Add
BLAST
Topological domaini1038 – 1198161Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. cilium Source: MGI
  3. cytoplasm Source: UniProtKB
  4. endoplasmic reticulum Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
  6. neuronal cell body Source: MGI
  7. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Atp2b2 null deficient mice are deaf.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11981198Plasma membrane calcium-transporting ATPase 2
PRO_0000046215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1094 – 10941Phosphothreonine; by PKC By similarity
Modified residuei1156 – 11561Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9R0K7.
PaxDbiQ9R0K7.
PRIDEiQ9R0K7.

PTM databases

PhosphoSiteiQ9R0K7.

Expressioni

Tissue specificityi

Mainly expressed in brain cortex. Found at low levels in heart, liver, lung and testis during late gestation. Also found in cochlea (stereocilia and outer wall of hair cells).

Gene expression databases

ArrayExpressiQ9R0K7.
BgeeiQ9R0K7.
CleanExiMM_ATP2B2.
GenevestigatoriQ9R0K7.

Interactioni

Subunit structurei

Interacts with PDZD11 By similarity.

Protein-protein interaction databases

BioGridi198250. 2 interactions.
IntActiQ9R0K7. 5 interactions.
MINTiMINT-4088651.
STRINGi10090.ENSMUSP00000098605.

Structurei

3D structure databases

ProteinModelPortaliQ9R0K7.
SMRiQ9R0K7. Positions 1078-1105.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1078 – 109518Calmodulin-binding subdomain A By similarity
Add
BLAST
Regioni1096 – 110510Calmodulin-binding subdomain B By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi294 – 2974Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
KOiK05850.
OrthoDBiEOG7SN8BN.
TreeFamiTF300330.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0K7-1 [UniParc]FASTAAdd to Basket

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MGDMTNSDFY SKNQRNESSH GGEFGCTMEE LRSLMELRGT EAVVKIKETY     50
GDTEAICRRL KTSPVEGLPG TAPDLEKRKQ IFGQNFIPPK KPKTFLQLVW 100
EALQDVTLII LEIAAIISLG LSFYHPPGES NEGCATAQGG AEDEGEAEAG 150
WIEGAAILLS VICVVLVTAF NDWSKEKQFR GLQSRIEQEQ KFTVVRAGQV 200
VQIPVAEIVV GDIAQIKYGD LLPADGLFIQ GNDLKIDESS LTGESDQVRK 250
SVDKDPMLLS GTHVMEGSGR MVVTAVGVNS QTGIIFTLLG AGGEEEEKKD 300
KKAKQQDGAA AMEMQPLKSA EGGDADDKKK ANMHKKEKSV LQGKLTKLAV 350
QIGKAGLVMS AITVIILVLY FTVDTFVVNK KPWLTECTPV YVQYFVKFFI 400
IGVTVLVVAV PEGLPLAVTI SLAYSVKKMM KDNNLVRHLD ACETMGNATA 450
ICSDKTGTLT TNRMTVVQAY VGDVHYKEIP DPSSINAKTL ELLVNAIAIN 500
SAYTTKILPP EKEGALPRQV GNKTECGLLG FVLDLRQDYE PVRSQMPEEK 550
LYKVYTFNSV RKSMSTVIKM PDESFRMYSK GASEIVLKKC CKILSGAGEA 600
RVFRPRDRDE MVKKVIEPMA CDGLRTICVA YRDFPSSPEP DWDNENDILN 650
ELTCICVVGI EDPVRPEVPE AIRKCQRAGI TVRMVTGDNI NTARAIAIKC 700
GIIHPGEDFL CLEGKEFNRR IRNEKGEIEQ ERIDKIWPKL RVLARSSPTD 750
KHTLVKGIID STHTEQRQVV AVTGDGTNDG PALKKADVGF AMGIAGTDVA 800
KEASDIILTD DNFSSIVKAV MWGRNVYDSI SKFLQFQLTV NVVAVIVAFT 850
GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTETLL LRKPYGRNKP 900
LISRTMMKNI LGHAVYQLTL IFTLLFVGEK MFQIDSGRNA PLHSPPSEHY 950
TIIFNTFVMM QLFNEINARK IHGERNVFDG IFRNPIFCTI VLGTFAIQIV 1000
IVQFGGKPFS CSPLQLDQWM WCIFIGLGEL VWGQVIATIP TSRLKFLKEA 1050
GRLTQKEEIP EEELNEDVEE IDHAERELRR GQILWFRGLN RIQTQIRVVK 1100
AFRSSLYEGL EKPESRTSIH NFMAHPEFRI EDSQPHIPLI DDTDLEEDAA 1150
LKQNSSPPSS LNKNNSAIDS GINLTTDTSK SATSSSPGSP IHSLETSL 1198
Length:1,198
Mass (Da):132,588
Last modified:June 1, 2001 - v2
Checksum:iBAA8CC28620D994B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti243 – 2431G → S in dfw. 1 Publication
Natural varianti412 – 4121E → K.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF053471 mRNA. Translation: AAC61255.1.
AB030737 mRNA. Translation: BAA83104.1.
AB030738 mRNA. Translation: BAA83105.1.
CCDSiCCDS20433.1.
RefSeqiNP_001031761.1. NM_001036684.2.
NP_033853.1. NM_009723.5.
UniGeneiMm.321755.
Mm.443564.

Genome annotation databases

EnsembliENSMUST00000089003; ENSMUSP00000086398; ENSMUSG00000030302.
ENSMUST00000101045; ENSMUSP00000098606; ENSMUSG00000030302.
GeneIDi11941.
KEGGimmu:11941.
UCSCiuc009dhn.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF053471 mRNA. Translation: AAC61255.1 .
AB030737 mRNA. Translation: BAA83104.1 .
AB030738 mRNA. Translation: BAA83105.1 .
CCDSi CCDS20433.1.
RefSeqi NP_001031761.1. NM_001036684.2.
NP_033853.1. NM_009723.5.
UniGenei Mm.321755.
Mm.443564.

3D structure databases

ProteinModelPortali Q9R0K7.
SMRi Q9R0K7. Positions 1078-1105.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198250. 2 interactions.
IntActi Q9R0K7. 5 interactions.
MINTi MINT-4088651.
STRINGi 10090.ENSMUSP00000098605.

PTM databases

PhosphoSitei Q9R0K7.

Proteomic databases

MaxQBi Q9R0K7.
PaxDbi Q9R0K7.
PRIDEi Q9R0K7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000089003 ; ENSMUSP00000086398 ; ENSMUSG00000030302 .
ENSMUST00000101045 ; ENSMUSP00000098606 ; ENSMUSG00000030302 .
GeneIDi 11941.
KEGGi mmu:11941.
UCSCi uc009dhn.1. mouse.

Organism-specific databases

CTDi 491.
MGIi MGI:105368. Atp2b2.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00510000046331.
HOGENOMi HOG000265623.
HOVERGENi HBG061286.
KOi K05850.
OrthoDBi EOG7SN8BN.
TreeFami TF300330.

Miscellaneous databases

ChiTaRSi Atp2b2. mouse.
NextBioi 280043.
PROi Q9R0K7.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9R0K7.
Bgeei Q9R0K7.
CleanExi MM_ATP2B2.
Genevestigatori Q9R0K7.

Family and domain databases

Gene3Di 1.20.1110.10. 3 hits.
InterProi IPR022141. ATP_Ca_trans_C.
IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mutations in a plasma membrane Ca2+-ATPase gene cause deafness in deafwaddler mice."
    Street V.A., McKee-Johnson J.W., Fonseca R.C., Tempel B.L., Noben-Trauth K.
    Nat. Genet. 19:390-394(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT DFW SER-243.
    Strain: C3H/HeJ.
  2. "A mouse with a point mutation in plasma membrane Ca2+-ATPase isoform 2 gene showed the reduced Ca2+ influx in cerebellar neurons."
    Ueno T., Kameyama K., Hirata M., Ogawa M., Hatsuse H., Takagaki Y., Ohmura M., Osawa N., Kudo Y.
    Neurosci. Res. 42:287-297(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-412.
    Strain: BALB/C WMS.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Balance and hearing deficits in mice with a null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2."
    Kozel P.J., Friedman R.A., Erway L.C., Yamoah E.N., Liu L.H., Riddle T., Duffy J.J., Doetschman T., Miller M.L., Cardell E.L., Shull G.E.
    J. Biol. Chem. 273:18693-18696(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiAT2B2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0K7
Secondary accession number(s): O88863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi