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Protein

Plasma membrane calcium-transporting ATPase 2

Gene

Atp2b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei454 – 45414-aspartylphosphate intermediateBy similarity
Metal bindingi775 – 7751MagnesiumBy similarity
Metal bindingi779 – 7791MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. calcium-dependent ATPase activity Source: MGI
  3. calcium ion binding Source: UniProtKB
  4. calcium-transporting ATPase activity Source: UniProtKB
  5. calmodulin binding Source: UniProtKB
  6. PDZ domain binding Source: UniProtKB
  7. protein C-terminus binding Source: UniProtKB

GO - Biological processi

  1. auditory receptor cell stereocilium organization Source: MGI
  2. calcium ion transmembrane transport Source: MGI
  3. calcium ion transport Source: DFLAT
  4. cell morphogenesis Source: MGI
  5. cellular calcium ion homeostasis Source: MGI
  6. cerebellar granule cell differentiation Source: MGI
  7. cerebellar Purkinje cell differentiation Source: MGI
  8. cerebellar Purkinje cell layer morphogenesis Source: MGI
  9. cerebellum development Source: MGI
  10. cGMP metabolic process Source: MGI
  11. cochlea development Source: DFLAT
  12. cytosolic calcium ion homeostasis Source: DFLAT
  13. detection of mechanical stimulus involved in sensory perception of sound Source: MGI
  14. inner ear development Source: MGI
  15. inner ear morphogenesis Source: MGI
  16. inner ear receptor cell differentiation Source: MGI
  17. lactation Source: MGI
  18. locomotion Source: MGI
  19. locomotory behavior Source: MGI
  20. neuromuscular process controlling balance Source: MGI
  21. neuron differentiation Source: UniProtKB
  22. organelle organization Source: MGI
  23. otolith mineralization Source: MGI
  24. positive regulation of calcium ion transport Source: MGI
  25. regulation of cell size Source: MGI
  26. regulation of synaptic plasticity Source: MGI
  27. sensory perception of sound Source: UniProtKB
  28. serotonin metabolic process Source: MGI
  29. synapse organization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.8. 3474.
ReactomeiREACT_327885. Reduction of cytosolic Ca++ levels.
REACT_338561. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 2 (EC:3.6.3.8)
Short name:
PMCA2
Alternative name(s):
Plasma membrane calcium ATPase isoform 2
Plasma membrane calcium pump isoform 2
Gene namesi
Name:Atp2b2
Synonyms:Pmca2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:105368. Atp2b2.

Subcellular locationi

  1. Cell junctionsynapse 1 Publication
  2. Cell membrane Sequence Analysis; Multi-pass membrane protein Sequence Analysis

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9493CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence AnalysisAdd
BLAST
Topological domaini116 – 15237ExtracellularSequence AnalysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Topological domaini174 – 345172CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei346 – 36520HelicalSequence AnalysisAdd
BLAST
Topological domaini366 – 39833ExtracellularSequence AnalysisAdd
BLAST
Transmembranei399 – 41618HelicalSequence AnalysisAdd
BLAST
Topological domaini417 – 830414CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei831 – 85020HelicalSequence AnalysisAdd
BLAST
Topological domaini851 – 86010ExtracellularSequence Analysis
Transmembranei861 – 88121HelicalSequence AnalysisAdd
BLAST
Topological domaini882 – 90120CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei902 – 92423HelicalSequence AnalysisAdd
BLAST
Topological domaini925 – 94218ExtracellularSequence AnalysisAdd
BLAST
Transmembranei943 – 96422HelicalSequence AnalysisAdd
BLAST
Topological domaini965 – 98319CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei984 – 100522HelicalSequence AnalysisAdd
BLAST
Topological domaini1006 – 101510ExtracellularSequence Analysis
Transmembranei1016 – 103722HelicalSequence AnalysisAdd
BLAST
Topological domaini1038 – 1198161CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. cilium Source: MGI
  4. cytoplasm Source: UniProtKB
  5. endoplasmic reticulum Source: MGI
  6. extracellular vesicular exosome Source: MGI
  7. integral component of membrane Source: UniProtKB-KW
  8. neuronal cell body Source: MGI
  9. plasma membrane Source: UniProtKB
  10. synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Atp2b2 null deficient mice are deaf.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 11981197Plasma membrane calcium-transporting ATPase 2PRO_0000046215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei319 – 3191PhosphoserineBy similarity
Modified residuei1094 – 10941Phosphothreonine; by PKCBy similarity
Modified residuei1133 – 11331PhosphoserineBy similarity
Modified residuei1143 – 11431PhosphothreonineBy similarity
Modified residuei1156 – 11561Phosphoserine; by PKABy similarity
Modified residuei1160 – 11601PhosphoserineBy similarity
Modified residuei1166 – 11661PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9R0K7.
PaxDbiQ9R0K7.
PRIDEiQ9R0K7.

PTM databases

PhosphoSiteiQ9R0K7.

Expressioni

Tissue specificityi

Expressed in the retina, with strongest levels in the inner plexiform layer, weaker levels in the outer plexiform layer, and very low levels in the proximal inner nuclear layer (PubMed:12209837). Specifically expressed in the following retinal cell types: rod bipolar cells, horizontal cells, amacrine cells and ganglion cells (PubMed:12209837). Also found in the cochlea (stereocilia and outer wall of hair cells) (at protein level) (PubMed:9697703). Strongly expressed in brain cortex. Found at low levels in heart, liver, lung and testis during late gestation (PubMed:9697703).2 Publications

Gene expression databases

BgeeiQ9R0K7.
CleanExiMM_ATP2B2.
ExpressionAtlasiQ9R0K7. baseline and differential.
GenevestigatoriQ9R0K7.

Interactioni

Subunit structurei

Interacts with PDZD11.By similarity

Protein-protein interaction databases

BioGridi198250. 2 interactions.
IntActiQ9R0K7. 5 interactions.
MINTiMINT-4088651.
STRINGi10090.ENSMUSP00000098605.

Structurei

3D structure databases

ProteinModelPortaliQ9R0K7.
SMRiQ9R0K7. Positions 1078-1105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1078 – 109518Calmodulin-binding subdomain ABy similarityAdd
BLAST
Regioni1096 – 110510Calmodulin-binding subdomain BBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi294 – 2974Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ9R0K7.
KOiK05850.
OrthoDBiEOG7SN8BN.
TreeFamiTF300330.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR030322. ATP2B2.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF247. PTHR24093:SF247. 1 hit.
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0K7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMTNSDFY SKNQRNESSH GGEFGCTMEE LRSLMELRGT EAVVKIKETY
60 70 80 90 100
GDTEAICRRL KTSPVEGLPG TAPDLEKRKQ IFGQNFIPPK KPKTFLQLVW
110 120 130 140 150
EALQDVTLII LEIAAIISLG LSFYHPPGES NEGCATAQGG AEDEGEAEAG
160 170 180 190 200
WIEGAAILLS VICVVLVTAF NDWSKEKQFR GLQSRIEQEQ KFTVVRAGQV
210 220 230 240 250
VQIPVAEIVV GDIAQIKYGD LLPADGLFIQ GNDLKIDESS LTGESDQVRK
260 270 280 290 300
SVDKDPMLLS GTHVMEGSGR MVVTAVGVNS QTGIIFTLLG AGGEEEEKKD
310 320 330 340 350
KKAKQQDGAA AMEMQPLKSA EGGDADDKKK ANMHKKEKSV LQGKLTKLAV
360 370 380 390 400
QIGKAGLVMS AITVIILVLY FTVDTFVVNK KPWLTECTPV YVQYFVKFFI
410 420 430 440 450
IGVTVLVVAV PEGLPLAVTI SLAYSVKKMM KDNNLVRHLD ACETMGNATA
460 470 480 490 500
ICSDKTGTLT TNRMTVVQAY VGDVHYKEIP DPSSINAKTL ELLVNAIAIN
510 520 530 540 550
SAYTTKILPP EKEGALPRQV GNKTECGLLG FVLDLRQDYE PVRSQMPEEK
560 570 580 590 600
LYKVYTFNSV RKSMSTVIKM PDESFRMYSK GASEIVLKKC CKILSGAGEA
610 620 630 640 650
RVFRPRDRDE MVKKVIEPMA CDGLRTICVA YRDFPSSPEP DWDNENDILN
660 670 680 690 700
ELTCICVVGI EDPVRPEVPE AIRKCQRAGI TVRMVTGDNI NTARAIAIKC
710 720 730 740 750
GIIHPGEDFL CLEGKEFNRR IRNEKGEIEQ ERIDKIWPKL RVLARSSPTD
760 770 780 790 800
KHTLVKGIID STHTEQRQVV AVTGDGTNDG PALKKADVGF AMGIAGTDVA
810 820 830 840 850
KEASDIILTD DNFSSIVKAV MWGRNVYDSI SKFLQFQLTV NVVAVIVAFT
860 870 880 890 900
GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTETLL LRKPYGRNKP
910 920 930 940 950
LISRTMMKNI LGHAVYQLTL IFTLLFVGEK MFQIDSGRNA PLHSPPSEHY
960 970 980 990 1000
TIIFNTFVMM QLFNEINARK IHGERNVFDG IFRNPIFCTI VLGTFAIQIV
1010 1020 1030 1040 1050
IVQFGGKPFS CSPLQLDQWM WCIFIGLGEL VWGQVIATIP TSRLKFLKEA
1060 1070 1080 1090 1100
GRLTQKEEIP EEELNEDVEE IDHAERELRR GQILWFRGLN RIQTQIRVVK
1110 1120 1130 1140 1150
AFRSSLYEGL EKPESRTSIH NFMAHPEFRI EDSQPHIPLI DDTDLEEDAA
1160 1170 1180 1190
LKQNSSPPSS LNKNNSAIDS GINLTTDTSK SATSSSPGSP IHSLETSL
Length:1,198
Mass (Da):132,588
Last modified:June 1, 2001 - v2
Checksum:iBAA8CC28620D994B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti243 – 2431G → S in dfw. 1 Publication
Natural varianti412 – 4121E → K.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053471 mRNA. Translation: AAC61255.1.
AB030737 mRNA. Translation: BAA83104.1.
AB030738 mRNA. Translation: BAA83105.1.
CCDSiCCDS20433.1.
RefSeqiNP_001031761.1. NM_001036684.2.
NP_033853.1. NM_009723.5.
UniGeneiMm.321755.
Mm.443564.

Genome annotation databases

EnsembliENSMUST00000089003; ENSMUSP00000086398; ENSMUSG00000030302.
ENSMUST00000101045; ENSMUSP00000098606; ENSMUSG00000030302.
GeneIDi11941.
KEGGimmu:11941.
UCSCiuc009dhn.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053471 mRNA. Translation: AAC61255.1.
AB030737 mRNA. Translation: BAA83104.1.
AB030738 mRNA. Translation: BAA83105.1.
CCDSiCCDS20433.1.
RefSeqiNP_001031761.1. NM_001036684.2.
NP_033853.1. NM_009723.5.
UniGeneiMm.321755.
Mm.443564.

3D structure databases

ProteinModelPortaliQ9R0K7.
SMRiQ9R0K7. Positions 1078-1105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198250. 2 interactions.
IntActiQ9R0K7. 5 interactions.
MINTiMINT-4088651.
STRINGi10090.ENSMUSP00000098605.

PTM databases

PhosphoSiteiQ9R0K7.

Proteomic databases

MaxQBiQ9R0K7.
PaxDbiQ9R0K7.
PRIDEiQ9R0K7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089003; ENSMUSP00000086398; ENSMUSG00000030302.
ENSMUST00000101045; ENSMUSP00000098606; ENSMUSG00000030302.
GeneIDi11941.
KEGGimmu:11941.
UCSCiuc009dhn.1. mouse.

Organism-specific databases

CTDi491.
MGIiMGI:105368. Atp2b2.

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00510000046331.
HOGENOMiHOG000265623.
HOVERGENiHBG061286.
InParanoidiQ9R0K7.
KOiK05850.
OrthoDBiEOG7SN8BN.
TreeFamiTF300330.

Enzyme and pathway databases

BRENDAi3.6.3.8. 3474.
ReactomeiREACT_327885. Reduction of cytosolic Ca++ levels.
REACT_338561. Ion transport by P-type ATPases.

Miscellaneous databases

ChiTaRSiAtp2b2. mouse.
NextBioi280043.
PROiQ9R0K7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9R0K7.
CleanExiMM_ATP2B2.
ExpressionAtlasiQ9R0K7. baseline and differential.
GenevestigatoriQ9R0K7.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
InterProiIPR030322. ATP2B2.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF247. PTHR24093:SF247. 1 hit.
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mutations in a plasma membrane Ca2+-ATPase gene cause deafness in deafwaddler mice."
    Street V.A., McKee-Johnson J.W., Fonseca R.C., Tempel B.L., Noben-Trauth K.
    Nat. Genet. 19:390-394(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT DFW SER-243.
    Strain: C3H/HeJ.
  2. "A mouse with a point mutation in plasma membrane Ca2+-ATPase isoform 2 gene showed the reduced Ca2+ influx in cerebellar neurons."
    Ueno T., Kameyama K., Hirata M., Ogawa M., Hatsuse H., Takagaki Y., Ohmura M., Osawa N., Kudo Y.
    Neurosci. Res. 42:287-297(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-412.
    Strain: BALB/C WMS.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Balance and hearing deficits in mice with a null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2."
    Kozel P.J., Friedman R.A., Erway L.C., Yamoah E.N., Liu L.H., Riddle T., Duffy J.J., Doetschman T., Miller M.L., Cardell E.L., Shull G.E.
    J. Biol. Chem. 273:18693-18696(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Cell-specific expression of plasma membrane calcium ATPase isoforms in retinal neurons."
    Krizaj D., Demarco S.J., Johnson J., Strehler E.E., Copenhagen D.R.
    J. Comp. Neurol. 451:1-21(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiAT2B2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0K7
Secondary accession number(s): O88863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 1, 2015
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.