Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9R0K7

- AT2B2_MOUSE

UniProt

Q9R0K7 - AT2B2_MOUSE

Protein

Plasma membrane calcium-transporting ATPase 2

Gene

Atp2b2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.1 Publication

    Catalytic activityi

    ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei454 – 45414-aspartylphosphate intermediateBy similarity
    Metal bindingi775 – 7751MagnesiumBy similarity
    Metal bindingi779 – 7791MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. calcium-dependent ATPase activity Source: MGI
    3. calcium ion binding Source: UniProtKB
    4. calcium-transporting ATPase activity Source: UniProtKB
    5. calmodulin binding Source: UniProtKB
    6. PDZ domain binding Source: UniProtKB
    7. protein C-terminus binding Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. auditory receptor cell stereocilium organization Source: MGI
    3. calcium ion transport Source: DFLAT
    4. cell morphogenesis Source: MGI
    5. cellular calcium ion homeostasis Source: MGI
    6. cerebellar granule cell differentiation Source: MGI
    7. cerebellar Purkinje cell differentiation Source: MGI
    8. cerebellar Purkinje cell layer morphogenesis Source: MGI
    9. cerebellum development Source: MGI
    10. cGMP metabolic process Source: MGI
    11. cochlea development Source: DFLAT
    12. cytosolic calcium ion homeostasis Source: DFLAT
    13. detection of mechanical stimulus involved in sensory perception of sound Source: MGI
    14. inner ear development Source: MGI
    15. inner ear morphogenesis Source: MGI
    16. inner ear receptor cell differentiation Source: MGI
    17. lactation Source: MGI
    18. locomotion Source: MGI
    19. locomotory behavior Source: MGI
    20. neuromuscular process controlling balance Source: MGI
    21. neuron differentiation Source: UniProtKB
    22. organelle organization Source: MGI
    23. otolith mineralization Source: MGI
    24. positive regulation of calcium ion transport Source: MGI
    25. regulation of cell size Source: MGI
    26. regulation of synaptic plasticity Source: MGI
    27. sensory perception of sound Source: UniProtKB
    28. serotonin metabolic process Source: MGI
    29. synapse organization Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasma membrane calcium-transporting ATPase 2 (EC:3.6.3.8)
    Short name:
    PMCA2
    Alternative name(s):
    Plasma membrane calcium ATPase isoform 2
    Plasma membrane calcium pump isoform 2
    Gene namesi
    Name:Atp2b2
    Synonyms:Pmca2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:105368. Atp2b2.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: MGI
    2. cilium Source: MGI
    3. cytoplasm Source: UniProtKB
    4. endoplasmic reticulum Source: MGI
    5. integral component of membrane Source: UniProtKB-KW
    6. neuronal cell body Source: MGI
    7. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Atp2b2 null deficient mice are deaf.

    Keywords - Diseasei

    Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11981198Plasma membrane calcium-transporting ATPase 2PRO_0000046215Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1094 – 10941Phosphothreonine; by PKCBy similarity
    Modified residuei1156 – 11561Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9R0K7.
    PaxDbiQ9R0K7.
    PRIDEiQ9R0K7.

    PTM databases

    PhosphoSiteiQ9R0K7.

    Expressioni

    Tissue specificityi

    Mainly expressed in brain cortex. Found at low levels in heart, liver, lung and testis during late gestation. Also found in cochlea (stereocilia and outer wall of hair cells).

    Gene expression databases

    ArrayExpressiQ9R0K7.
    BgeeiQ9R0K7.
    CleanExiMM_ATP2B2.
    GenevestigatoriQ9R0K7.

    Interactioni

    Subunit structurei

    Interacts with PDZD11.By similarity

    Protein-protein interaction databases

    BioGridi198250. 2 interactions.
    IntActiQ9R0K7. 5 interactions.
    MINTiMINT-4088651.
    STRINGi10090.ENSMUSP00000098605.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0K7.
    SMRiQ9R0K7. Positions 1078-1105.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9494CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini116 – 15237ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini174 – 345172CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini366 – 39833ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini417 – 830414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini851 – 86010ExtracellularSequence Analysis
    Topological domaini882 – 90120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini925 – 94218ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini965 – 98319CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1006 – 101510ExtracellularSequence Analysis
    Topological domaini1038 – 1198161CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei95 – 11521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei153 – 17321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei346 – 36520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei399 – 41618HelicalSequence AnalysisAdd
    BLAST
    Transmembranei831 – 85020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei861 – 88121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei902 – 92423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei943 – 96422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei984 – 100522HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1016 – 103722HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1078 – 109518Calmodulin-binding subdomain ABy similarityAdd
    BLAST
    Regioni1096 – 110510Calmodulin-binding subdomain BBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi294 – 2974Poly-Glu

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00510000046331.
    HOGENOMiHOG000265623.
    HOVERGENiHBG061286.
    KOiK05850.
    OrthoDBiEOG7SN8BN.
    TreeFamiTF300330.

    Family and domain databases

    Gene3Di1.20.1110.10. 3 hits.
    InterProiIPR022141. ATP_Ca_trans_C.
    IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF12424. ATP_Ca_trans_C. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R0K7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDMTNSDFY SKNQRNESSH GGEFGCTMEE LRSLMELRGT EAVVKIKETY     50
    GDTEAICRRL KTSPVEGLPG TAPDLEKRKQ IFGQNFIPPK KPKTFLQLVW 100
    EALQDVTLII LEIAAIISLG LSFYHPPGES NEGCATAQGG AEDEGEAEAG 150
    WIEGAAILLS VICVVLVTAF NDWSKEKQFR GLQSRIEQEQ KFTVVRAGQV 200
    VQIPVAEIVV GDIAQIKYGD LLPADGLFIQ GNDLKIDESS LTGESDQVRK 250
    SVDKDPMLLS GTHVMEGSGR MVVTAVGVNS QTGIIFTLLG AGGEEEEKKD 300
    KKAKQQDGAA AMEMQPLKSA EGGDADDKKK ANMHKKEKSV LQGKLTKLAV 350
    QIGKAGLVMS AITVIILVLY FTVDTFVVNK KPWLTECTPV YVQYFVKFFI 400
    IGVTVLVVAV PEGLPLAVTI SLAYSVKKMM KDNNLVRHLD ACETMGNATA 450
    ICSDKTGTLT TNRMTVVQAY VGDVHYKEIP DPSSINAKTL ELLVNAIAIN 500
    SAYTTKILPP EKEGALPRQV GNKTECGLLG FVLDLRQDYE PVRSQMPEEK 550
    LYKVYTFNSV RKSMSTVIKM PDESFRMYSK GASEIVLKKC CKILSGAGEA 600
    RVFRPRDRDE MVKKVIEPMA CDGLRTICVA YRDFPSSPEP DWDNENDILN 650
    ELTCICVVGI EDPVRPEVPE AIRKCQRAGI TVRMVTGDNI NTARAIAIKC 700
    GIIHPGEDFL CLEGKEFNRR IRNEKGEIEQ ERIDKIWPKL RVLARSSPTD 750
    KHTLVKGIID STHTEQRQVV AVTGDGTNDG PALKKADVGF AMGIAGTDVA 800
    KEASDIILTD DNFSSIVKAV MWGRNVYDSI SKFLQFQLTV NVVAVIVAFT 850
    GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTETLL LRKPYGRNKP 900
    LISRTMMKNI LGHAVYQLTL IFTLLFVGEK MFQIDSGRNA PLHSPPSEHY 950
    TIIFNTFVMM QLFNEINARK IHGERNVFDG IFRNPIFCTI VLGTFAIQIV 1000
    IVQFGGKPFS CSPLQLDQWM WCIFIGLGEL VWGQVIATIP TSRLKFLKEA 1050
    GRLTQKEEIP EEELNEDVEE IDHAERELRR GQILWFRGLN RIQTQIRVVK 1100
    AFRSSLYEGL EKPESRTSIH NFMAHPEFRI EDSQPHIPLI DDTDLEEDAA 1150
    LKQNSSPPSS LNKNNSAIDS GINLTTDTSK SATSSSPGSP IHSLETSL 1198
    Length:1,198
    Mass (Da):132,588
    Last modified:June 1, 2001 - v2
    Checksum:iBAA8CC28620D994B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti243 – 2431G → S in dfw. 1 Publication
    Natural varianti412 – 4121E → K.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF053471 mRNA. Translation: AAC61255.1.
    AB030737 mRNA. Translation: BAA83104.1.
    AB030738 mRNA. Translation: BAA83105.1.
    CCDSiCCDS20433.1.
    RefSeqiNP_001031761.1. NM_001036684.2.
    NP_033853.1. NM_009723.5.
    UniGeneiMm.321755.
    Mm.443564.

    Genome annotation databases

    EnsembliENSMUST00000089003; ENSMUSP00000086398; ENSMUSG00000030302.
    ENSMUST00000101045; ENSMUSP00000098606; ENSMUSG00000030302.
    GeneIDi11941.
    KEGGimmu:11941.
    UCSCiuc009dhn.1. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF053471 mRNA. Translation: AAC61255.1 .
    AB030737 mRNA. Translation: BAA83104.1 .
    AB030738 mRNA. Translation: BAA83105.1 .
    CCDSi CCDS20433.1.
    RefSeqi NP_001031761.1. NM_001036684.2.
    NP_033853.1. NM_009723.5.
    UniGenei Mm.321755.
    Mm.443564.

    3D structure databases

    ProteinModelPortali Q9R0K7.
    SMRi Q9R0K7. Positions 1078-1105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198250. 2 interactions.
    IntActi Q9R0K7. 5 interactions.
    MINTi MINT-4088651.
    STRINGi 10090.ENSMUSP00000098605.

    PTM databases

    PhosphoSitei Q9R0K7.

    Proteomic databases

    MaxQBi Q9R0K7.
    PaxDbi Q9R0K7.
    PRIDEi Q9R0K7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000089003 ; ENSMUSP00000086398 ; ENSMUSG00000030302 .
    ENSMUST00000101045 ; ENSMUSP00000098606 ; ENSMUSG00000030302 .
    GeneIDi 11941.
    KEGGi mmu:11941.
    UCSCi uc009dhn.1. mouse.

    Organism-specific databases

    CTDi 491.
    MGIi MGI:105368. Atp2b2.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00510000046331.
    HOGENOMi HOG000265623.
    HOVERGENi HBG061286.
    KOi K05850.
    OrthoDBi EOG7SN8BN.
    TreeFami TF300330.

    Miscellaneous databases

    ChiTaRSi Atp2b2. mouse.
    NextBioi 280043.
    PROi Q9R0K7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R0K7.
    Bgeei Q9R0K7.
    CleanExi MM_ATP2B2.
    Genevestigatori Q9R0K7.

    Family and domain databases

    Gene3Di 1.20.1110.10. 3 hits.
    InterProi IPR022141. ATP_Ca_trans_C.
    IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF12424. ATP_Ca_trans_C. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutations in a plasma membrane Ca2+-ATPase gene cause deafness in deafwaddler mice."
      Street V.A., McKee-Johnson J.W., Fonseca R.C., Tempel B.L., Noben-Trauth K.
      Nat. Genet. 19:390-394(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT DFW SER-243.
      Strain: C3H/HeJ.
    2. "A mouse with a point mutation in plasma membrane Ca2+-ATPase isoform 2 gene showed the reduced Ca2+ influx in cerebellar neurons."
      Ueno T., Kameyama K., Hirata M., Ogawa M., Hatsuse H., Takagaki Y., Ohmura M., Osawa N., Kudo Y.
      Neurosci. Res. 42:287-297(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-412.
      Strain: BALB/C WMS.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Balance and hearing deficits in mice with a null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2."
      Kozel P.J., Friedman R.A., Erway L.C., Yamoah E.N., Liu L.H., Riddle T., Duffy J.J., Doetschman T., Miller M.L., Cardell E.L., Shull G.E.
      J. Biol. Chem. 273:18693-18696(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiAT2B2_MOUSE
    AccessioniPrimary (citable) accession number: Q9R0K7
    Secondary accession number(s): O88863
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3