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Protein

E3 ubiquitin-protein ligase XIAP

Gene

Xiap

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi299ZincPROSITE-ProRule annotation1
Metal bindingi302ZincPROSITE-ProRule annotation1
Metal bindingi319ZincPROSITE-ProRule annotation1
Metal bindingi326ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri449 – 484RING-typePROSITE-ProRule annotationAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiI32.004.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase XIAP (EC:6.3.2.-)
Alternative name(s):
Baculoviral IAP repeat-containing protein 4
IAP homolog A
Inhibitor of apoptosis protein 3
Short name:
IAP-3
Short name:
rIAP-3
Short name:
rIAP3
X-linked inhibitor of apoptosis protein
Short name:
X-linked IAP
Gene namesi
Name:Xiap
Synonyms:Api3, Birc4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi620692. Xiap.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: TLE3 promotes its nuclear localization.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001223541 – 496E3 ubiquitin-protein ligase XIAPAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87Phosphoserine; by PKBBy similarity1
Cross-linki321Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki327Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei449S-nitrosocysteineBy similarity1

Post-translational modificationi

S-Nitrosylation down-regulates its E3 ubiquitin-protein ligase activity.By similarity
Autoubiquitinated and degraded by the proteasome in apoptotic cells.By similarity
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, S-nitrosylation, Ubl conjugation

Proteomic databases

PaxDbiQ9R0I6.
PRIDEiQ9R0I6.

PTM databases

iPTMnetiQ9R0I6.
PhosphoSitePlusiQ9R0I6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000006967.
ExpressionAtlasiQ9R0I6. baseline and differential.
GenevisibleiQ9R0I6. RN.

Interactioni

Subunit structurei

Monomer, and homodimer. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with TAB1/MAP3K7IP1 and AIFM1. Interaction with SMAC hinders binding of TAB1/MAP3K7IP1 and AIFM1. Interacts with TCF25 and COMMD1. Interacts with SEPT4 isoform 6, but not with other SEPT4 isoforms. Interacts with RIP1, RIP2, RIP3, RIP4, CCS and USP19. Interacts (via BIR 2 domain and BIR 3 domain) with HAX1 (via C-terminus) and this interaction blocks ubiquitination of XIAP/BIRC4. Interacts with the monomeric form of BIRC5/survivin (By similarity). Interacts with TLE3 and TCF7L2/TCF4 (By similarity).By similarity

GO - Molecular functioni

  • protease binding Source: RGD
  • scaffold protein binding Source: RGD

Protein-protein interaction databases

BioGridi248914. 4 interactors.
MINTiMINT-2839635.
STRINGi10116.ENSRNOP00000009336.

Structurei

3D structure databases

ProteinModelPortaliQ9R0I6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati26 – 93BIR 1Add BLAST68
Repeati163 – 230BIR 2Add BLAST68
Repeati264 – 329BIR 3Add BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 149Interaction with caspase-7By similarity9

Domaini

The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with DIABLO/SMAC and PRSS25 are mediated by the second and third BIR domains (By similarity).By similarity

Sequence similaritiesi

Belongs to the IAP family.Curated
Contains 3 BIR repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri449 – 484RING-typePROSITE-ProRule annotationAdd BLAST36

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1101. Eukaryota.
ENOG410YPNM. LUCA.
GeneTreeiENSGT00500000044782.
HOGENOMiHOG000232059.
HOVERGENiHBG004848.
InParanoidiQ9R0I6.
KOiK04725.
PhylomeDBiQ9R0I6.

Family and domain databases

CDDicd00022. BIR. 3 hits.
Gene3Di1.10.1170.10. 4 hits.
InterProiIPR001370. BIR_rpt.
IPR001841. Znf_RING.
[Graphical view]
PfamiPF00653. BIR. 3 hits.
[Graphical view]
SMARTiSM00238. BIR. 3 hits.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS01282. BIR_REPEAT_1. 3 hits.
PS50143. BIR_REPEAT_2. 3 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0I6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFNSFEGSR TVVPADTNKD EEFVEEFNRL KTFANFPSSS PVSASTLARA
60 70 80 90 100
GFLYTGEGDT VQCFSCHAAV DRWQYGDSAV GRHRRISPNC RFINGFYFEN
110 120 130 140 150
GATQSTSPGI QNGQYKSENC VGNRNHFALD RPSETHADYL LRTGQVVDIS
160 170 180 190 200
DTIYPRNPAM CSEEARLKTF QNWPDYAHLS PRELASAGLY YTGIDDQVQC
210 220 230 240 250
FCCGGKLKNW EPCDRAWSEH RRHFPNCFFV LGRNVNVRSE SGVSSDRNFP
260 270 280 290 300
NSTNSPRNPA MAEYDARIVT FGTWLYSVNK EQLARAGFYA LGEGDKVKCF
310 320 330 340 350
HCGGGLTDWK PSEDPWEQHA KWYPGCKYLL DEKGQEYINN IHLTHSLGES
360 370 380 390 400
VVRTAEKTPS VTKKIDDTIF QNPMVQEAIR MGFNFKDIKK TMEEKLQTSG
410 420 430 440 450
SNYLSLEVLI ADLVSAQKDN SQDESSQTSL QKDISTEEQL RRLQEEKLCK
460 470 480 490
ICMDRNIAIV FVPCGHLVTC KQCAEAVDKC PMCCTVITFK QKIFMS
Length:496
Mass (Da):56,073
Last modified:May 1, 2000 - v1
Checksum:iE250E3C77461A469
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033366 mRNA. Translation: BAA85304.1.
RefSeqiNP_071567.1. NM_022231.2.
XP_006257570.1. XM_006257508.3.
XP_006257571.1. XM_006257509.3.
XP_017457637.1. XM_017602148.1.
XP_017457638.1. XM_017602149.1.
XP_017457639.1. XM_017602150.1.
XP_017457640.1. XM_017602151.1.
UniGeneiRn.91239.

Genome annotation databases

EnsembliENSRNOT00000009336; ENSRNOP00000009336; ENSRNOG00000006967.
GeneIDi63879.
KEGGirno:63879.
UCSCiRGD:620692. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033366 mRNA. Translation: BAA85304.1.
RefSeqiNP_071567.1. NM_022231.2.
XP_006257570.1. XM_006257508.3.
XP_006257571.1. XM_006257509.3.
XP_017457637.1. XM_017602148.1.
XP_017457638.1. XM_017602149.1.
XP_017457639.1. XM_017602150.1.
XP_017457640.1. XM_017602151.1.
UniGeneiRn.91239.

3D structure databases

ProteinModelPortaliQ9R0I6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248914. 4 interactors.
MINTiMINT-2839635.
STRINGi10116.ENSRNOP00000009336.

Protein family/group databases

MEROPSiI32.004.

PTM databases

iPTMnetiQ9R0I6.
PhosphoSitePlusiQ9R0I6.

Proteomic databases

PaxDbiQ9R0I6.
PRIDEiQ9R0I6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009336; ENSRNOP00000009336; ENSRNOG00000006967.
GeneIDi63879.
KEGGirno:63879.
UCSCiRGD:620692. rat.

Organism-specific databases

CTDi331.
RGDi620692. Xiap.

Phylogenomic databases

eggNOGiKOG1101. Eukaryota.
ENOG410YPNM. LUCA.
GeneTreeiENSGT00500000044782.
HOGENOMiHOG000232059.
HOVERGENiHBG004848.
InParanoidiQ9R0I6.
KOiK04725.
PhylomeDBiQ9R0I6.

Miscellaneous databases

PROiQ9R0I6.

Gene expression databases

BgeeiENSRNOG00000006967.
ExpressionAtlasiQ9R0I6. baseline and differential.
GenevisibleiQ9R0I6. RN.

Family and domain databases

CDDicd00022. BIR. 3 hits.
Gene3Di1.10.1170.10. 4 hits.
InterProiIPR001370. BIR_rpt.
IPR001841. Znf_RING.
[Graphical view]
PfamiPF00653. BIR. 3 hits.
[Graphical view]
SMARTiSM00238. BIR. 3 hits.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS01282. BIR_REPEAT_1. 3 hits.
PS50143. BIR_REPEAT_2. 3 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXIAP_RAT
AccessioniPrimary (citable) accession number: Q9R0I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.