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Q9R0G8

- NRK_MOUSE

UniProt

Q9R0G8 - NRK_MOUSE

Protein

Nik-related protein kinase

Gene

Nrk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei177 – 1771Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: MGI
    3. protein serine/threonine kinase activity Source: UniProtKB-KW
    4. small GTPase regulator activity Source: InterPro

    GO - Biological processi

    1. activation of JNKK activity Source: MGI
    2. negative regulation of cell proliferation Source: MGI
    3. parturition Source: MGI
    4. regulation of spongiotrophoblast cell proliferation Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nik-related protein kinase (EC:2.7.11.1)
    Alternative name(s):
    Nck-interacting kinase-like embryo specific kinase
    Short name:
    NESK
    Short name:
    NIK-like embryo-specific kinase
    Gene namesi
    Name:Nrk
    Synonyms:Nesk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1351326. Nrk.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi54 – 541K → E: Kinase inactivation. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14551455Nik-related protein kinasePRO_0000250512Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei847 – 8471PhosphoserineBy similarity
    Modified residuei850 – 8501PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9R0G8.
    PRIDEiQ9R0G8.

    PTM databases

    PhosphoSiteiQ9R0G8.

    Expressioni

    Developmental stagei

    Predominantly expressed in skeletal muscle during embryogenesis. Expression was detected in the myotome at 10.5 dpc and, thereafter, was observed in developing skeletal musculature from 11.5 to 13.5 dpc and increased from 15 to 17 dpc. However, expression in skeletal muscle was not observed in adults. Its expression may be down-regulated as development proceeds.3 Publications

    Gene expression databases

    ArrayExpressiQ9R0G8.
    BgeeiQ9R0G8.
    CleanExiMM_NRK.
    GenevestigatoriQ9R0G8.

    Interactioni

    Protein-protein interaction databases

    BioGridi205128. 1 interaction.
    IntActiQ9R0G8. 1 interaction.
    MINTiMINT-4130820.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9R0G8.
    SMRiQ9R0G8. Positions 14-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 313289Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1138 – 1425288CNHPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili716 – 75035Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi398 – 531134Gln-richAdd
    BLAST
    Compositional biasi806 – 8138Poly-Gln
    Compositional biasi958 – 1059102Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CNH domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000114927.
    HOGENOMiHOG000049277.
    HOVERGENiHBG080612.
    KOiK16313.
    OMAiEDIFNQH.
    TreeFamiTF105138.

    Family and domain databases

    InterProiIPR001180. Citron.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00780. CNH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00036. CNH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS50219. CNH. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9R0G8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGPGSWRDK EVTDLGQLPD PTGIFSLDKA IGLGTYGRIF LGIHEKTGSL     50
    VAVKVMSARK TPLPEIGRRV RVNKYQKSVG WRYSDEEEDL RTELNLLRKY 100
    SFHKNIVTFY GAFFKLNPPG HQHQLWMVME LCAAGSVTDV VRMTRNQSLK 150
    EDWIAYICRE ILQGLAHLHA HQVIHRDIKG QNVLLTHDAE VKIVDFGVSA 200
    QVSRTNGRRN SFIGTPYWMA PEVIHCDEDP RCSYDYRSDV WSVGITAIEM 250
    AEGAPPLCKL QPLEALCVIL REAAPKVKSS GWSRKFQNFM ENCMIKNFLF 300
    RPTSGNMLLH PFVHDIKNER RVVESLTKHL TGIIQKREKK GIPVAFEGEE 350
    AAKEQYITRR FRGPSCTPEL LRVPTSSRCR PLRVLHGEPP QPRWLPDQED 400
    PQDQELQQLQ KAAGVFMPLH SQDNTSKLFP KQVEVAPYLR GAAQVVMPVL 450
    VQVEAPPQVS KAAQMLKSLP TQDNKATSPE VQAPVAEGQQ AQHEALETEQ 500
    PKDLDQVPEE FQGQDRAPEQ PRQGQAAEQQ QIHNPVPEQP PEEDREPEQA 550
    EVQEEAVEPP QAEIEDKEPE VVQVHAQVLL PLLSQNRHVL LPLHLDRQLL 600
    IPVGEQNEEV PRAQAWDLEA SRAVGAVQAL IEGLSRDLLR APNAFVTKPL 650
    GPLQIFLENL STDGFYTEPE PTQKKKSKVA SLRKAIAKRL RPKRFRAKAL 700
    WRLEDFEFSD VETSRRRRHR RWEDIFNQHE EQLRRVENDR EDDSSDNDEV 750
    FHSIQAEVQI EPHAANPAGN EVHERSAPMP CNRNRTHRVK FSPSVGEEEP 800
    SLEEAQPQQQ QQQPMNIRPR NCLNPQNFQA QSDSSSEEDS PVTRRKSQSS 850
    PPYSTIDQKL LIDIHVPDGF KVGKISPPVY LTNEWVGYNA LSEIFWDDWI 900
    MPTRPARPPE EDGDYVELYD ADANANGDEE VANGAYEDPR DGANGHDDMN 950
    NQLDQANGYE GHGAAGYNGG DVGGNHGAAF NGPRANYPRA GILKNGHNDG 1000
    RALNRGAFGV FGDNAARAFH GAAGEAGAAF GNHGANRGNG RGNRNREANG 1050
    RNEENGAFGR DQHVFPEFEH EESDRGTETS DSIALEITSF DGEQNSGRPV 1100
    SSTTMGFPIG RSSPRGSDFG SDISYNSPIL HVYEKDFSSE VYCGSLWGVN 1150
    LLLGTQSHLY LMDRSGKAEI VKLIKRRPFR QIQVVEQLNL LITISGKKNR 1200
    LRVYHLSWLR NKILNNDPKS KKRQKAMRKK EEACKAIDKL IGCEHFSVLQ 1250
    HEETTYIAVA VKSSIHLFAW APKSFDENTA IKVFPTRDLK PLTVDLAVGS 1300
    EKTLKIFFSS ANGYHIIDAE SEVMSEVTLP NNNVVILPDC LGLGVMLSLN 1350
    AEAASEEANE QLLKKILDVW KDIPSSVAFE CTKRITGWDQ KAIEVRSLQS 1400
    TILENELKRR SIKKLRFLCA RGDKMFFAST LSNDHSRVYL MSLGKLEELH 1450
    RSYAV 1455
    Length:1,455
    Mass (Da):163,647
    Last modified:July 27, 2011 - v2
    Checksum:i7588DBE31B15ED91
    GO

    Sequence cautioni

    The sequence AAH68311.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti274 – 2741A → V in BAA84943. (PubMed:10559491)Curated
    Sequence conflicti397 – 3971D → N in BAA84943. (PubMed:10559491)Curated
    Sequence conflicti467 – 4671K → R in BAA87066. (PubMed:10801798)Curated
    Sequence conflicti894 – 8941I → T in AAH68311. (PubMed:15489334)Curated
    Sequence conflicti1008 – 10081F → S in AAH68311. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB020741 mRNA. Translation: BAA84943.1.
    AB035267 mRNA. Translation: BAA87066.1.
    BC139029 mRNA. Translation: AAI39030.1.
    BC139031 mRNA. Translation: AAI39032.1.
    BC068311 mRNA. Translation: AAH68311.1. Sequence problems.
    AK041377 mRNA. Translation: BAC30923.1.
    CCDSiCCDS41143.1.
    RefSeqiNP_038752.2. NM_013724.2.
    UniGeneiMm.482319.

    Genome annotation databases

    EnsembliENSMUST00000064937; ENSMUSP00000063397; ENSMUSG00000052854.
    GeneIDi27206.
    KEGGimmu:27206.
    UCSCiuc009ujx.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB020741 mRNA. Translation: BAA84943.1 .
    AB035267 mRNA. Translation: BAA87066.1 .
    BC139029 mRNA. Translation: AAI39030.1 .
    BC139031 mRNA. Translation: AAI39032.1 .
    BC068311 mRNA. Translation: AAH68311.1 . Sequence problems.
    AK041377 mRNA. Translation: BAC30923.1 .
    CCDSi CCDS41143.1.
    RefSeqi NP_038752.2. NM_013724.2.
    UniGenei Mm.482319.

    3D structure databases

    ProteinModelPortali Q9R0G8.
    SMRi Q9R0G8. Positions 14-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 205128. 1 interaction.
    IntActi Q9R0G8. 1 interaction.
    MINTi MINT-4130820.

    PTM databases

    PhosphoSitei Q9R0G8.

    Proteomic databases

    PaxDbi Q9R0G8.
    PRIDEi Q9R0G8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000064937 ; ENSMUSP00000063397 ; ENSMUSG00000052854 .
    GeneIDi 27206.
    KEGGi mmu:27206.
    UCSCi uc009ujx.2. mouse.

    Organism-specific databases

    CTDi 203447.
    MGIi MGI:1351326. Nrk.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000114927.
    HOGENOMi HOG000049277.
    HOVERGENi HBG080612.
    KOi K16313.
    OMAi EDIFNQH.
    TreeFami TF105138.

    Miscellaneous databases

    ChiTaRSi NRK. mouse.
    NextBioi 305075.
    PROi Q9R0G8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9R0G8.
    Bgeei Q9R0G8.
    CleanExi MM_NRK.
    Genevestigatori Q9R0G8.

    Family and domain databases

    InterProi IPR001180. Citron.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00780. CNH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00036. CNH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS50219. CNH. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nrk: a murine X-linked NIK (Nck-interacting kinase)-related kinase gene expressed in skeletal muscle."
      Kanai-Azuma M., Kanai Y., Okamoto M., Hayashi Y., Yonekawa H., Yazaki K.
      Mech. Dev. 89:155-159(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Strain: BALB/c.
      Tissue: Embryo.
    2. "NESK, a member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and is expressed during the late stages of embryogenesis."
      Nakano K., Yamauchi J., Nakagawa K., Itoh H., Kitamura N.
      J. Biol. Chem. 275:20533-20539(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-54, FUNCTION, DEVELOPMENTAL STAGE.
      Tissue: Embryo.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Brain and Embryo.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-334.
      Strain: C57BL/6J.
      Tissue: Thymus.
    5. "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family."
      Nakano K., Kanai-Azuma M., Kanai Y., Moriyama K., Yazaki K., Hayashi Y., Kitamura N.
      Exp. Cell Res. 287:219-227(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LYS-54, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiNRK_MOUSE
    AccessioniPrimary (citable) accession number: Q9R0G8
    Secondary accession number(s): B2RSW5
    , Q6NV55, Q8C9S9, Q9R0S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3