Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9R0G8 (NRK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nik-related protein kinase

EC=2.7.11.1
Alternative name(s):
Nck-interacting kinase-like embryo specific kinase
Short name=NESK
Short name=NIK-like embryo-specific kinase
Gene names
Name:Nrk
Synonyms:Nesk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1455 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway. Ref.2 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Developmental stage

Predominantly expressed in skeletal muscle during embryogenesis. Expression was detected in the myotome at 10.5 dpc and, thereafter, was observed in developing skeletal musculature from 11.5 to 13.5 dpc and increased from 15 to 17 dpc. However, expression in skeletal muscle was not observed in adults. Its expression may be down-regulated as development proceeds. Ref.1 Ref.2 Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 CNH domain.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAH68311.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14551455Nik-related protein kinase
PRO_0000250512

Regions

Domain25 – 313289Protein kinase
Domain1138 – 1425288CNH
Nucleotide binding31 – 399ATP By similarity
Coiled coil716 – 75035 Potential
Compositional bias398 – 531134Gln-rich
Compositional bias806 – 8138Poly-Gln
Compositional bias958 – 1059102Gly-rich

Sites

Active site1771Proton acceptor By similarity
Binding site541ATP By similarity

Amino acid modifications

Modified residue8471Phosphoserine By similarity
Modified residue8501Phosphoserine By similarity

Experimental info

Mutagenesis541K → E: Kinase inactivation. Ref.2 Ref.5
Sequence conflict2741A → V in BAA84943. Ref.1
Sequence conflict3971D → N in BAA84943. Ref.1
Sequence conflict4671K → R in BAA87066. Ref.2
Sequence conflict8941I → T in AAH68311. Ref.3
Sequence conflict10081F → S in AAH68311. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9R0G8 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 7588DBE31B15ED91

FASTA1,455163,647
        10         20         30         40         50         60 
MAGPGSWRDK EVTDLGQLPD PTGIFSLDKA IGLGTYGRIF LGIHEKTGSL VAVKVMSARK 

        70         80         90        100        110        120 
TPLPEIGRRV RVNKYQKSVG WRYSDEEEDL RTELNLLRKY SFHKNIVTFY GAFFKLNPPG 

       130        140        150        160        170        180 
HQHQLWMVME LCAAGSVTDV VRMTRNQSLK EDWIAYICRE ILQGLAHLHA HQVIHRDIKG 

       190        200        210        220        230        240 
QNVLLTHDAE VKIVDFGVSA QVSRTNGRRN SFIGTPYWMA PEVIHCDEDP RCSYDYRSDV 

       250        260        270        280        290        300 
WSVGITAIEM AEGAPPLCKL QPLEALCVIL REAAPKVKSS GWSRKFQNFM ENCMIKNFLF 

       310        320        330        340        350        360 
RPTSGNMLLH PFVHDIKNER RVVESLTKHL TGIIQKREKK GIPVAFEGEE AAKEQYITRR 

       370        380        390        400        410        420 
FRGPSCTPEL LRVPTSSRCR PLRVLHGEPP QPRWLPDQED PQDQELQQLQ KAAGVFMPLH 

       430        440        450        460        470        480 
SQDNTSKLFP KQVEVAPYLR GAAQVVMPVL VQVEAPPQVS KAAQMLKSLP TQDNKATSPE 

       490        500        510        520        530        540 
VQAPVAEGQQ AQHEALETEQ PKDLDQVPEE FQGQDRAPEQ PRQGQAAEQQ QIHNPVPEQP 

       550        560        570        580        590        600 
PEEDREPEQA EVQEEAVEPP QAEIEDKEPE VVQVHAQVLL PLLSQNRHVL LPLHLDRQLL 

       610        620        630        640        650        660 
IPVGEQNEEV PRAQAWDLEA SRAVGAVQAL IEGLSRDLLR APNAFVTKPL GPLQIFLENL 

       670        680        690        700        710        720 
STDGFYTEPE PTQKKKSKVA SLRKAIAKRL RPKRFRAKAL WRLEDFEFSD VETSRRRRHR 

       730        740        750        760        770        780 
RWEDIFNQHE EQLRRVENDR EDDSSDNDEV FHSIQAEVQI EPHAANPAGN EVHERSAPMP 

       790        800        810        820        830        840 
CNRNRTHRVK FSPSVGEEEP SLEEAQPQQQ QQQPMNIRPR NCLNPQNFQA QSDSSSEEDS 

       850        860        870        880        890        900 
PVTRRKSQSS PPYSTIDQKL LIDIHVPDGF KVGKISPPVY LTNEWVGYNA LSEIFWDDWI 

       910        920        930        940        950        960 
MPTRPARPPE EDGDYVELYD ADANANGDEE VANGAYEDPR DGANGHDDMN NQLDQANGYE 

       970        980        990       1000       1010       1020 
GHGAAGYNGG DVGGNHGAAF NGPRANYPRA GILKNGHNDG RALNRGAFGV FGDNAARAFH 

      1030       1040       1050       1060       1070       1080 
GAAGEAGAAF GNHGANRGNG RGNRNREANG RNEENGAFGR DQHVFPEFEH EESDRGTETS 

      1090       1100       1110       1120       1130       1140 
DSIALEITSF DGEQNSGRPV SSTTMGFPIG RSSPRGSDFG SDISYNSPIL HVYEKDFSSE 

      1150       1160       1170       1180       1190       1200 
VYCGSLWGVN LLLGTQSHLY LMDRSGKAEI VKLIKRRPFR QIQVVEQLNL LITISGKKNR 

      1210       1220       1230       1240       1250       1260 
LRVYHLSWLR NKILNNDPKS KKRQKAMRKK EEACKAIDKL IGCEHFSVLQ HEETTYIAVA 

      1270       1280       1290       1300       1310       1320 
VKSSIHLFAW APKSFDENTA IKVFPTRDLK PLTVDLAVGS EKTLKIFFSS ANGYHIIDAE 

      1330       1340       1350       1360       1370       1380 
SEVMSEVTLP NNNVVILPDC LGLGVMLSLN AEAASEEANE QLLKKILDVW KDIPSSVAFE 

      1390       1400       1410       1420       1430       1440 
CTKRITGWDQ KAIEVRSLQS TILENELKRR SIKKLRFLCA RGDKMFFAST LSNDHSRVYL 

      1450 
MSLGKLEELH RSYAV 

« Hide

References

« Hide 'large scale' references
[1]"Nrk: a murine X-linked NIK (Nck-interacting kinase)-related kinase gene expressed in skeletal muscle."
Kanai-Azuma M., Kanai Y., Okamoto M., Hayashi Y., Yonekawa H., Yazaki K.
Mech. Dev. 89:155-159(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: BALB/c.
Tissue: Embryo.
[2]"NESK, a member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and is expressed during the late stages of embryogenesis."
Nakano K., Yamauchi J., Nakagawa K., Itoh H., Kitamura N.
J. Biol. Chem. 275:20533-20539(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-54, FUNCTION, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain and Embryo.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-334.
Strain: C57BL/6J.
Tissue: Thymus.
[5]"Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family."
Nakano K., Kanai-Azuma M., Kanai Y., Moriyama K., Yazaki K., Hayashi Y., Kitamura N.
Exp. Cell Res. 287:219-227(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LYS-54, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB020741 mRNA. Translation: BAA84943.1.
AB035267 mRNA. Translation: BAA87066.1.
BC139029 mRNA. Translation: AAI39030.1.
BC139031 mRNA. Translation: AAI39032.1.
BC068311 mRNA. Translation: AAH68311.1. Sequence problems.
AK041377 mRNA. Translation: BAC30923.1.
CCDSCCDS41143.1.
RefSeqNP_038752.2. NM_013724.2.
UniGeneMm.482319.

3D structure databases

ProteinModelPortalQ9R0G8.
SMRQ9R0G8. Positions 14-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid205128. 1 interaction.
IntActQ9R0G8. 1 interaction.
MINTMINT-4130820.

PTM databases

PhosphoSiteQ9R0G8.

Proteomic databases

PaxDbQ9R0G8.
PRIDEQ9R0G8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000064937; ENSMUSP00000063397; ENSMUSG00000052854.
GeneID27206.
KEGGmmu:27206.
UCSCuc009ujx.2. mouse.

Organism-specific databases

CTD203447.
MGIMGI:1351326. Nrk.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00740000114927.
HOGENOMHOG000049277.
HOVERGENHBG080612.
KOK16313.
OMAEDIFNQH.
TreeFamTF105138.

Gene expression databases

ArrayExpressQ9R0G8.
BgeeQ9R0G8.
CleanExMM_NRK.
GenevestigatorQ9R0G8.

Family and domain databases

InterProIPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNRK. mouse.
NextBio305075.
PROQ9R0G8.
SOURCESearch...

Entry information

Entry nameNRK_MOUSE
AccessionPrimary (citable) accession number: Q9R0G8
Secondary accession number(s): B2RSW5 expand/collapse secondary AC list , Q6NV55, Q8C9S9, Q9R0S4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot