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Q9R0G8

- NRK_MOUSE

UniProt

Q9R0G8 - NRK_MOUSE

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Protein

Nik-related protein kinase

Gene
Nrk, Nesk
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATP By similarity
Active sitei177 – 1771Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: MGI
  3. receptor signaling protein serine/threonine kinase activity Source: RefGenome
  4. small GTPase regulator activity Source: InterPro

GO - Biological processi

  1. activation of JNKK activity Source: MGI
  2. negative regulation of cell proliferation Source: MGI
  3. parturition Source: MGI
  4. regulation of spongiotrophoblast cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nik-related protein kinase (EC:2.7.11.1)
Alternative name(s):
Nck-interacting kinase-like embryo specific kinase
Short name:
NESK
Short name:
NIK-like embryo-specific kinase
Gene namesi
Name:Nrk
Synonyms:Nesk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1351326. Nrk.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi54 – 541K → E: Kinase inactivation. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14551455Nik-related protein kinasePRO_0000250512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei847 – 8471Phosphoserine By similarity
Modified residuei850 – 8501Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9R0G8.
PRIDEiQ9R0G8.

PTM databases

PhosphoSiteiQ9R0G8.

Expressioni

Developmental stagei

Predominantly expressed in skeletal muscle during embryogenesis. Expression was detected in the myotome at 10.5 dpc and, thereafter, was observed in developing skeletal musculature from 11.5 to 13.5 dpc and increased from 15 to 17 dpc. However, expression in skeletal muscle was not observed in adults. Its expression may be down-regulated as development proceeds.3 Publications

Gene expression databases

ArrayExpressiQ9R0G8.
BgeeiQ9R0G8.
CleanExiMM_NRK.
GenevestigatoriQ9R0G8.

Interactioni

Protein-protein interaction databases

BioGridi205128. 1 interaction.
IntActiQ9R0G8. 1 interaction.
MINTiMINT-4130820.

Structurei

3D structure databases

ProteinModelPortaliQ9R0G8.
SMRiQ9R0G8. Positions 14-339.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 313289Protein kinaseAdd
BLAST
Domaini1138 – 1425288CNHAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili716 – 75035 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi398 – 531134Gln-richAdd
BLAST
Compositional biasi806 – 8138Poly-Gln
Compositional biasi958 – 1059102Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 CNH domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000114927.
HOGENOMiHOG000049277.
HOVERGENiHBG080612.
KOiK16313.
OMAiEDIFNQH.
TreeFamiTF105138.

Family and domain databases

InterProiIPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9R0G8-1 [UniParc]FASTAAdd to Basket

« Hide

MAGPGSWRDK EVTDLGQLPD PTGIFSLDKA IGLGTYGRIF LGIHEKTGSL     50
VAVKVMSARK TPLPEIGRRV RVNKYQKSVG WRYSDEEEDL RTELNLLRKY 100
SFHKNIVTFY GAFFKLNPPG HQHQLWMVME LCAAGSVTDV VRMTRNQSLK 150
EDWIAYICRE ILQGLAHLHA HQVIHRDIKG QNVLLTHDAE VKIVDFGVSA 200
QVSRTNGRRN SFIGTPYWMA PEVIHCDEDP RCSYDYRSDV WSVGITAIEM 250
AEGAPPLCKL QPLEALCVIL REAAPKVKSS GWSRKFQNFM ENCMIKNFLF 300
RPTSGNMLLH PFVHDIKNER RVVESLTKHL TGIIQKREKK GIPVAFEGEE 350
AAKEQYITRR FRGPSCTPEL LRVPTSSRCR PLRVLHGEPP QPRWLPDQED 400
PQDQELQQLQ KAAGVFMPLH SQDNTSKLFP KQVEVAPYLR GAAQVVMPVL 450
VQVEAPPQVS KAAQMLKSLP TQDNKATSPE VQAPVAEGQQ AQHEALETEQ 500
PKDLDQVPEE FQGQDRAPEQ PRQGQAAEQQ QIHNPVPEQP PEEDREPEQA 550
EVQEEAVEPP QAEIEDKEPE VVQVHAQVLL PLLSQNRHVL LPLHLDRQLL 600
IPVGEQNEEV PRAQAWDLEA SRAVGAVQAL IEGLSRDLLR APNAFVTKPL 650
GPLQIFLENL STDGFYTEPE PTQKKKSKVA SLRKAIAKRL RPKRFRAKAL 700
WRLEDFEFSD VETSRRRRHR RWEDIFNQHE EQLRRVENDR EDDSSDNDEV 750
FHSIQAEVQI EPHAANPAGN EVHERSAPMP CNRNRTHRVK FSPSVGEEEP 800
SLEEAQPQQQ QQQPMNIRPR NCLNPQNFQA QSDSSSEEDS PVTRRKSQSS 850
PPYSTIDQKL LIDIHVPDGF KVGKISPPVY LTNEWVGYNA LSEIFWDDWI 900
MPTRPARPPE EDGDYVELYD ADANANGDEE VANGAYEDPR DGANGHDDMN 950
NQLDQANGYE GHGAAGYNGG DVGGNHGAAF NGPRANYPRA GILKNGHNDG 1000
RALNRGAFGV FGDNAARAFH GAAGEAGAAF GNHGANRGNG RGNRNREANG 1050
RNEENGAFGR DQHVFPEFEH EESDRGTETS DSIALEITSF DGEQNSGRPV 1100
SSTTMGFPIG RSSPRGSDFG SDISYNSPIL HVYEKDFSSE VYCGSLWGVN 1150
LLLGTQSHLY LMDRSGKAEI VKLIKRRPFR QIQVVEQLNL LITISGKKNR 1200
LRVYHLSWLR NKILNNDPKS KKRQKAMRKK EEACKAIDKL IGCEHFSVLQ 1250
HEETTYIAVA VKSSIHLFAW APKSFDENTA IKVFPTRDLK PLTVDLAVGS 1300
EKTLKIFFSS ANGYHIIDAE SEVMSEVTLP NNNVVILPDC LGLGVMLSLN 1350
AEAASEEANE QLLKKILDVW KDIPSSVAFE CTKRITGWDQ KAIEVRSLQS 1400
TILENELKRR SIKKLRFLCA RGDKMFFAST LSNDHSRVYL MSLGKLEELH 1450
RSYAV 1455
Length:1,455
Mass (Da):163,647
Last modified:July 27, 2011 - v2
Checksum:i7588DBE31B15ED91
GO

Sequence cautioni

The sequence AAH68311.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741A → V in BAA84943. 1 Publication
Sequence conflicti397 – 3971D → N in BAA84943. 1 Publication
Sequence conflicti467 – 4671K → R in BAA87066. 1 Publication
Sequence conflicti894 – 8941I → T in AAH68311. 1 Publication
Sequence conflicti1008 – 10081F → S in AAH68311. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB020741 mRNA. Translation: BAA84943.1.
AB035267 mRNA. Translation: BAA87066.1.
BC139029 mRNA. Translation: AAI39030.1.
BC139031 mRNA. Translation: AAI39032.1.
BC068311 mRNA. Translation: AAH68311.1. Sequence problems.
AK041377 mRNA. Translation: BAC30923.1.
CCDSiCCDS41143.1.
RefSeqiNP_038752.2. NM_013724.2.
UniGeneiMm.482319.

Genome annotation databases

EnsembliENSMUST00000064937; ENSMUSP00000063397; ENSMUSG00000052854.
GeneIDi27206.
KEGGimmu:27206.
UCSCiuc009ujx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB020741 mRNA. Translation: BAA84943.1 .
AB035267 mRNA. Translation: BAA87066.1 .
BC139029 mRNA. Translation: AAI39030.1 .
BC139031 mRNA. Translation: AAI39032.1 .
BC068311 mRNA. Translation: AAH68311.1 . Sequence problems.
AK041377 mRNA. Translation: BAC30923.1 .
CCDSi CCDS41143.1.
RefSeqi NP_038752.2. NM_013724.2.
UniGenei Mm.482319.

3D structure databases

ProteinModelPortali Q9R0G8.
SMRi Q9R0G8. Positions 14-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 205128. 1 interaction.
IntActi Q9R0G8. 1 interaction.
MINTi MINT-4130820.

PTM databases

PhosphoSitei Q9R0G8.

Proteomic databases

PaxDbi Q9R0G8.
PRIDEi Q9R0G8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000064937 ; ENSMUSP00000063397 ; ENSMUSG00000052854 .
GeneIDi 27206.
KEGGi mmu:27206.
UCSCi uc009ujx.2. mouse.

Organism-specific databases

CTDi 203447.
MGIi MGI:1351326. Nrk.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000114927.
HOGENOMi HOG000049277.
HOVERGENi HBG080612.
KOi K16313.
OMAi EDIFNQH.
TreeFami TF105138.

Miscellaneous databases

ChiTaRSi NRK. mouse.
NextBioi 305075.
PROi Q9R0G8.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9R0G8.
Bgeei Q9R0G8.
CleanExi MM_NRK.
Genevestigatori Q9R0G8.

Family and domain databases

InterProi IPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nrk: a murine X-linked NIK (Nck-interacting kinase)-related kinase gene expressed in skeletal muscle."
    Kanai-Azuma M., Kanai Y., Okamoto M., Hayashi Y., Yonekawa H., Yazaki K.
    Mech. Dev. 89:155-159(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Embryo.
  2. "NESK, a member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and is expressed during the late stages of embryogenesis."
    Nakano K., Yamauchi J., Nakagawa K., Itoh H., Kitamura N.
    J. Biol. Chem. 275:20533-20539(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-54, FUNCTION, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Embryo.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-334.
    Strain: C57BL/6J.
    Tissue: Thymus.
  5. "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family."
    Nakano K., Kanai-Azuma M., Kanai Y., Moriyama K., Yazaki K., Hayashi Y., Kitamura N.
    Exp. Cell Res. 287:219-227(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-54, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiNRK_MOUSE
AccessioniPrimary (citable) accession number: Q9R0G8
Secondary accession number(s): B2RSW5
, Q6NV55, Q8C9S9, Q9R0S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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